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Citations for Merck :
1501 - 1550 of 3358 citations for 2 Amino 6 chloro 9 3 5 di O p toluoyl beta D 2 deoxyribofurnanosyl purine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Each lobe was segmented so cryosections would fit on the 6,200 x 6,400 µm areas of the Codelink-activated microscope slides and frozen in −30°C 2-Methylbutane (Merck, cat.no.: M32631-1L). The frozen liver samples were embedded in cryomolds (10×10 mm ...
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bioRxiv - Microbiology 2022Quote: ... Five duplicate plates were spread individually by six gradient diluted soil suspensions from 10−2 to 10−7 dilution on 1/10 TSA medium (Tryptic Soy Agar, Merck, Darmstadt, Germany) and incubated under both aerobic and anaerobic conditions at 28 °C for two weeks ...
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bioRxiv - Plant Biology 2022Quote: ... disposable 200 μl pipette tips were fitted with 2 plugs of C18 octadecyl 47 mm Disks 2215 (Empore™) material and 1mg:10 μg of LiChroprep® RP-18 (Merck) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were subjected to sonication on ice (10 cycles consisting of 10-s impulses at an output 10% followed by 20-s intervals) followed by incubation for 20 min at 37 °C in the presence of micrococcal nuclease (2 units/sample; Merck, cat. # N3755) and CaCl2 (at 10 mM) ...
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bioRxiv - Cancer Biology 2022Quote: ... (MW 360 kDa) and (Hydroxypropyl)-methylcellulose (HPMC) (viscosity 40-60 cP, 2 % in H2O (20 C) were purchased from Merck (Darmstadt, Germany). Methanol ...
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bioRxiv - Molecular Biology 2023Quote: ... Each lobe was segmented so cryosections would fit on the 6,200 x 6,400 µm areas of the Codelink-activated microscope or Visium slides and frozen in -30°C 2-Methylbutane (Merck, cat.no.: M32631-1L). For spatial experiments ...
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bioRxiv - Neuroscience 2024Quote: ... Semithin sections (1–2 μm thick) were obtained from the surface of the block and stained with 1% toluidine blue (Merck, 115930, Germany) in 1% sodium borate (Panreac ...
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bioRxiv - Neuroscience 2023Quote: GNAO1+/G203R#2 cells at the stage of neuronal rosettes (30 day of differentiation) were fixed in 4% paraformaldehyde solution (Merck Life Sciences) for 15 min at room temperature and washed thrice with 1X PBS (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Semithin sections (1–2 μm thick) were obtained from the surface of the block and stained with 1% toluidine blue (Merck, 115930, Germany) in 1% sodium borate (Panreac ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated with 50 µM inhibitor (dissolved at 50 mM concentration in 100% DMSO) or 2 mM ATPψS (Merck Life Science UK) at room temperature for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... The culture was grown for 2 hr at 37°C and stocks were made from the original plate by adding glycerol (Merck & Co., USA) to the final concentration of 15% and stored at -70°C ...
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bioRxiv - Neuroscience 2023Quote: Blood was collected in 2 ml EDTA tubes that were prepared with 100µl protease inhibitor (Pefabloc® SC Plus, Merck KGaA, Germany). These tubes were centrifuged immediately after venepuncture for 15 min at 4⁰C and 3200g ...
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bioRxiv - Molecular Biology 2023Quote: ... Media was concentrated further to a final volume of 1-2 ml using 10 KDa Amicon spin filters (Merck Millipore, MA, USA) by centrifugation at 3,500 g ...
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bioRxiv - Biochemistry 2023Quote: ... samples were concentrated to a volume of approximately 2 mL using Amicon® Ultra centrifugal filters with a NMWC of 10 kDa (cat# UFC801024, Merck Millipore).
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bioRxiv - Genetics 2023Quote: ... after adding 2 g activated (i.e., baked at 300°C for 2h) molecular sieves (5Å, 45-60 mesh size, Merck, KGaA, Darmstadt, Germany). The molecular sieves were filtered out by loading the extract into a glass funnel (50 mm inner diameter ...
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bioRxiv - Neuroscience 2022Quote: ... freezed 15 min at −80°C and thawed for 15 min at room temperature following overnight incubation in 2% TritonX-100 (Merck Millipore, 1086031000) PBS for permeabilization ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Semithin sections (1–2 μm thick) were obtained from the surface of the block and stained with 1% toluidine blue (Merck, 115930, Germany) in 1% sodium borate (Panreac ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
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bioRxiv - Microbiology 2024Quote: ... The conidial samples were then suspended by 2 mL of sterile water and filtered through Miracloth cloths (pore size 22-25 μm, Merck KgaA, Germany) to remove mycelial fragments ...
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bioRxiv - Immunology 2024Quote: ... and nucleocapsid (NC)] were monitored in the plasma samples using Millipore’s MILLIPLEX® MAP SARS-CoV-2 Antigen EMD panel IgG and IgA (Merck, Darmstadt, Germany), according with the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 200.000 D1 cells were plated in a 96-well plate 2 hours prior to stimulation with Yoda1 (40 µM Merck Life Science).
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bioRxiv - Biophysics 2024Quote: ... Purified protein samples were concentrated to 5-6 mg ml−1 in an aqueous buffer solution (20 mM Tris, 250 mM NaCl, 20% glycerol, and 10 mM 2- mercaptoethanol) and 0.6 M Trehalose (Merck KGaA, Darmstadt, Germany) when samples were lyophilized ...
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bioRxiv - Developmental Biology 2024Quote: ... ten E6 morulae were transferred to each recipient cows (n = 2) following synchronization with initial intramuscular injection of gonadotropin-releasing hormone (Fertagyl; Merck, Rahway, NJ), standard 7-day vaginal controlled internal drug release (EAZI-BREED CIDR ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated O/N at 4 °C with the following primary antibodies: mouse anti-glial fibrillary acidic protein (GFAP; Merck, Madrid, Spain; #MAB360; 1:1000) and rabbit anti-ionized calcium-binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Plant Biology 2021Quote: Arabidopsis 14-3-3 isoforms Epsilon and Lambda were expressed using the pGEX-4T1 vector (Merck), as a translational fusion with glutathione-S-transferase (GST ...
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bioRxiv - Bioengineering 2021Quote: ... and the amount of ALP measured using SigmaFast™ p-Nitrophenyl phosphate (pNPP) tablets (Merck KGaA). A standard curve drawn with serial dilution of pNPP and a known quantity of ALP from bovine intestinal mucosa (Merck KGaA) ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Proteins of interest were detected using the following primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a PVDF membrane (0.45µm Immobilon®-P, Merck Millipore Ltd., VIC, Australia) at 100 V in chilled transfer buffer containing 25 mM Tris ...
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bioRxiv - Plant Biology 2022Quote: ... The separated proteins were transferred to polyvinylidene difluoride membranes (Immobilon-P; Merck Millipore, Burlington, MA, USA) and analyzed with the following antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto methanol pre-activated polyvinylidene fluoride (PVDF) membranes (IPFL00010, Immobilon-P; Merck-Millipore). Membranes transferred from TGX Stain-Free™ gels were imaged and quantified for posterior normalization with a ChemiDoc XRS+ (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: Western blot analysis was carried out by semidry electroblotting on immobilon-P transfer membrane from Merck KGaA (Darmstadt ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred onto methanol-pretreated Immobilon®-P PVDF Membrane (0.45 μm pore size) (Merck; IPVH00010) using BOLT gel transfer cassettes and BOLT transfer buffer (Invitrogen™ ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were blotted onto a 0.45 µm PDVF Immobilon-P membrane (Merck Millipore, ref. IPVH00010) after electrophoresis ...
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bioRxiv - Plant Biology 2024Quote: ... Gel was transferred to polyvinylidene difluoride membranes (IPVH00010 Immobilon-P Membrane; Merck, Rahway, New Jersey, USA). Membrane was blocked with 1 or 2% BSA in 1× PBS solution with 0.2% Tween 20 (PBST ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred on a PolyVinyliDene Fluoride (PVDF) membrane (Immobilon-P, Merck Millipore, Burlington, USA) using liquid transfer with 20 % methanol at 100V ...
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bioRxiv - Plant Biology 2024Quote: ... and the resolved crosslinkers were transferred onto a polyvinylidene fluoride (PVDF) membrane (Immobilon-P, IPVH00010, Merck) using a Mini Blot Module (B1000 ...
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bioRxiv - Biochemistry 2020Quote: ... coverslips were coated with poly-D-lysine (Merck Millipore) before seeding the cells ...
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bioRxiv - Neuroscience 2020Quote: 100 nM Cytochalasin D (Merck Calbiochem, Cat. No. 250255), 100 μM CK-666 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... were treated with adding 2μM cytochalasin D (Merck Millipore) and 0.2μM Nocodazole (Merck Millipore) ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... on poly-D-lysine 50 µg/ml (Merck Millipore) and laminin 10 µg/ml (SigmaAldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... Following inhibitors were used: Cytochalasin D (1 µM, Merck) and Y-27632 (30 µM ...
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bioRxiv - Plant Biology 2024Quote: ... and 25µl of 2,4-D (5mg/ml, Sigma-Merck) were mixed and placed in the hole created ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... neurons were seeded on poly-D-lysine (Merck, P1149) and LAM/Laminin (Merck ...
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bioRxiv - Cell Biology 2020Quote: ... and 1x phosphatase inhibitor 3 (Merck). Cell debris was removed by pelleting at 5000g for 10mins ...
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bioRxiv - Immunology 2021Quote: ... 3-Methyladenine (3MA, 5mM; Merck Millipore) or Anakinra (500ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-mGluR2/3 (Merck Millipore), rabbit anti-CRTAC1 (Merck Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... primarily fixed in 3% glutaraldehyde (Merck) in 0.1 M sodium phosphate buffer with pH 7.2 at 4°C for 1 h ...
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bioRxiv - Physiology 2024Quote: ... - 3% horse serum (Merck, ref H1270) PBS solution for 1 h ...