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Citations for Merck :
1451 - 1500 of 1932 citations for L Valine 13C5 95 97%; 15N 96 99%; 2 3 D2 97%+ since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The supernatant was then concentrated to 100-200 μL using centrifugal filter units with a 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) by centrifugation at 5000 g at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... with PBS pH 7.4 for 10 min at 4400 x g and 4°C using an Amicon Ultra-4 concentrator with 3 kDa cutoff (Merck Millipore). The degree of labelling (DOL = 1.96 ...
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bioRxiv - Cell Biology 2022Quote: ... they were mechanically disrupted by a 21G needle inside a 3 ml DLD dissociation solution (PluriSTEM® Dispase-II Solution, Liberase, #5401119001 DNase I, #10104159001, all from Merck), and incubated for 20-30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Clear lysates were subsequently filtered through 10 kDa followed by 3 kDa Amicon Ultra-0.5 centrifugal filters (Merck KGaA, Darmstadt, Germany) to generate sub-cellular fractions with molecular size of larger than 10 kDa ...
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bioRxiv - Cell Biology 2023Quote: J774A.1 macrophages with or without infection (3 hour) were lysed in a specific lysis buffer containing DCP-Bio1 (Merck # NS1226) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting sample of holo-SmLPMO10A was concentrated to ~ 150 μM using an Amicon ®Ultra centrifugation filter (3 kDa cut-off, Merck) and the sample was split into three equal volumes of ~ 500 μL ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was removed (around 25 ml) and either concentrated 10-fold with Amicon 3 kDa spin columns (Merck, cat. #UFC900308) and brought to a final volume of 2 ml for subsequent Western blot analysis (Fig.1B ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) using centrifugation at 5000 g ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed in PBS for 3 times and Alexa Fluor 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) was added for immunostaining in the dark for 2 hours at RT ...
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bioRxiv - Plant Biology 2023Quote: ... the dried pellets were redissolved in 80 % methanol (1µL/mg) and 3 µL were analysed by HPTLC on a Silica Gel 60 plate (Merck, Darmstadt, Germany). The mobile phase used was ethyl acetate ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.6) for 30 min at RT before incubation with blocking solution consisting of 3% bovine serum albumin (Sigma, Merck, Germany, #A9418) and 0.3% Triton X-100 in PBS 1X ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted fractions were concentrated and dialyzed against PBS using Amicon Ultra-15 Centrifugal Filter Units with a 3 kDa MWCO (Merck Millipore). Endotoxins were removed using High Capacity Endotoxin Removal Spin Columns (Pierce) ...
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bioRxiv - Evolutionary Biology 2023Quote: Total RNA was extracted from ∼150 injected animals (3 days old planulae) with the aid of Tri- Reagent® (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and dialyzed against cold sterile PBS using an Amicon Ultra-15 Centrifugal Filter Unit with a 3 kDa MWCO (Merck Millipore). The resulting product was then passed through a 0.22 µM filter.
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were subsequently washed 3 times for 10 minutes with PB containing 0.2 % Triton X-100 (Merck, Darmstadt, Germany, 108603; PBT). Next ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were subsequently washed 3 times for 10 minutes with PB containing 0.2 % Triton X-100 (Merck, Darmstadt, Germany, 108603; PBT). Next ...
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bioRxiv - Bioengineering 2024Quote: ... Culture supernatants were harvested at 3 days post-transfection and filtered through a 0.45 μm filter (Merck Millipore, Burlington, MA, USA). All proteins were purified with a Talon metal affinity resin (Takara Bio USA ...
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bioRxiv - Biophysics 2019Quote: ... for 2 h and buffer exchange with centrifugal filter devices (10,000 MWCO, Amicon, Merck Millipore) into storage buffer ...
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bioRxiv - Molecular Biology 2021Quote: WT MtbRho and the M495L mutant were overexpressed in Rosetta 2(DE3) cells (Merck-Millipore) harboring the appropriate pET28b derivative and purified following published protocols35 with minor modifications ...
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bioRxiv - Developmental Biology 2019Quote: ... The next day FGF ligands (to 5 nM) and heparin (to 2 μg/ml; Merck) were added ...
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bioRxiv - Biochemistry 2020Quote: ... and a SeQuant ZIC-HILIC precolumn (5 μm particles, 20 × 2 mm) (Merck, Darmstadt, Germany) using a linear gradient of mobile phase A (5 mM NH4OAc in acetonitrile/H2O (5/95 ...
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bioRxiv - Biochemistry 2021Quote: Ethanol (absolute for analysis) and 2-propanol (for analysis) were obtained from Merck (Darmstadt, Germany). Sodium chloride (≥99.8%) ...
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bioRxiv - Cell Biology 2021Quote: 6 μL of 70 kDa FITC-dextran in EGM-2 (5 mg/mL, Merck, #46945) was added to each vessel ...
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bioRxiv - Cell Biology 2021Quote: ... Intracellularly applied drug: the membrane impermeable G-protein blocker GDP-β-S (2 mM, Merck) (Farkas et al. ...
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bioRxiv - Cell Biology 2020Quote: ... at a final concentration of 10 ng/ml and phosphatase inhibitor cocktail-2 (P5726, Merck) at 1% (v/v) ...
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bioRxiv - Immunology 2022Quote: ... EB retained in the brain tissue was extracted by immersion in 2 ml formamide (Merck) at 37°C in the dark for 48 h ...
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bioRxiv - Biophysics 2022Quote: ... bis-acrylamide (2% w/w) and ammonium persulfate (0.05% w/v) (all from Merck, Germany) in 10 mM Tris-buffer (pH 7.48) ...
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bioRxiv - Cancer Biology 2022Quote: ... cell-free samples were fixed for 1 hour at room temperature with 2% glutaraldehyde (Merck) in 0.1M cacodylate buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Acetylthiocholine iodide (ATCh) and 5,5’-dithio-bis-2-nitrobenzoic acid (DTNB) were obtained from Merck (https://www.sigmaaldrich.com/united-kingdom.html ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μL of extract containing total lipids were separated on TLC (Silica Gel 60, Merck) by using petroleum ether:diethyl ether (90:10 ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos from in vitro culture were fixed in a solution containing 2% paraformaldehyde (PFA, Merck) for 20 min at 37° C [41] and ex vivo flushed embryos were fixed in 4% PFA over night at 4° C ...
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bioRxiv - Immunology 2022Quote: Nose and throat swabs were collected in 2 ml transport medium containing 15% sucrose (Merck), 2.5 µg/ml Amphotericin B ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μl of terminated reaction mix were spotted onto polyethyl-enimine-cellulose plates (Merck, Germany). ATP and released phosphate were then separated chromatographically in a buffer of 0.5 M LiCl ...
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bioRxiv - Microbiology 2023Quote: ... 300 μL of aqueous phase was added to 500 μL of 2-propanol (Merck, Germany) and kept overnight at −20°C for RNA precipitation ...
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bioRxiv - Microbiology 2022Quote: ... After testing for SARS-CoV-2 by PCR test (COBAS 6800, Merck México, Mexico city), they were classified as positive (AP ...
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bioRxiv - Microbiology 2022Quote: ... Maize was cultured in ¼ Hoagland’s medium (Hoagland’s No. 2 Basal Salt Mixture, Sigma-Aldrich/Merck), pH adjusted to 5.6-5.8 with KOH ...
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bioRxiv - Immunology 2022Quote: ... Peritoneal cavity cells were obtained by lavage with 5 mL PBS + 2 mM EDTA (Merck). Following gentle massage ...
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bioRxiv - Neuroscience 2022Quote: ... Two hundred microliters of medium containing 2 μM human insulin (Merck, Sigma-Aldrich Cat# I9278) or medium only was added to the thoraxes for 10 min (the final concentration of insulin was 1 μM) ...
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bioRxiv - Microbiology 2023Quote: All compounds (zaprinast, heparin, artemisinin, chloroquine, brefeldin A and Torin 2) were sourced from Merck KGaA ...
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bioRxiv - Developmental Biology 2023Quote: ... rat anti tyrosinated-α-tubulin 1:250 (MAB1864-I, clone YL1/2, EMD Millipore/Merck), mouse anti-Armadillo/β-Catenin 1:50 (N27A1 ...
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bioRxiv - Microbiology 2023Quote: ... 300 µL of aqueous phase was added to 500 µL of 2-propanol (Merck, Germany) and kept overnight at −20°C ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 200µM of 1-phenyl-2-thiourea (PTU, ref. n°P7629, Merck/Sigma-Aldrich) to avoid pigmentation ...
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bioRxiv - Cancer Biology 2023Quote: ... femurs and tibias in FACS buffer (PBS supplemented with 2% fetal calf serum (FCS; Merck) and 2 mM EDTA (Merck) ...
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bioRxiv - Genetics 2023Quote: ... was added to each along with a single sterile 2 mm glass bead (Merck, Germany) to each tube ...
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bioRxiv - Biophysics 2023Quote: ... RNA from yeast diluted in PBS (0.7 and 2 mg/mL; Sigma-Aldrich, Merck #10109223001).
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bioRxiv - Bioengineering 2023Quote: ... followed by incubation in 10 mL of 2 U/mL dispase solution (D4693; Merck KGaA) at 4 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded onto a 2 ml column containing Ni-NTA His·Bind resin (Merck). The column was first washed with a solution containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.12 U/μL RNase Inhibitor) inside a 2 mL Sorenson Dolphin microcentrifuge tube (Merck). The mixture containing the nuclei (800 μL ...
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bioRxiv - Synthetic Biology 2023Quote: ... 500 µl 0.2 µg/ml 4’,6’-diamidino-2-phenylindole (DAPI, Merck, catalog no. D9542) in PBS was added for 15 min at RT ...