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Citations for Merck :
101 - 150 of 196 citations for TOLLIP Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The concentration of peptides was measured by a Direct Detect Spectrophotometer (Merck, Darmstadt, Germany), and solutions were made up to 100 μg in 100 μl of injection solution prior to desalting.
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bioRxiv - Cell Biology 2023Quote: ... peptide concentration was estimated via Direct Detect® Infrared Spectrometer (Merck, Bayswater, VIC, Australia), and samples were transferred to HPLC vials for proteomics analysis.
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were diluted in blocking solution together with DAPI (0.01 mg/mL) (Merck Life Science) and incubated for 20 minutes at room temperature in a dark ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for 30 min and blocked for 1 h with blocking solution (Merck-Millipore, Burlington, MS, USA) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by a 3 h blocking step with 5 % NGS (G9023, Merck, Darmstadt Germany) in the respective washing buffer ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were blocked with 2% BSA in PBS with 0.1% Tween-20 (Merck; blocking buffer) and incubated with primary antibodies diluted in blocking buffer overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Primary antibodies were diluted in the corresponding blocking solution anti-HA for ZnT9 (H3663, Sigma-Merck), and anti-GAPDH (ab8245 ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were then vacuum concentrated (Labconco Centrivap) and peptides purified by C18 ZipTips® (Merck) prior to analysis by LC-MS/MS or LC-SWATH-MS ...
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bioRxiv - Systems Biology 2022Quote: ... The procedure was performed by manufacturer’s instructions including peptide purification with C18 ZipTips® (Merck). The eluted peptides were snap frozen and lyophilized before reconstitution in 0.1% formic acid for HPLC-MS/MS.
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bioRxiv - Cell Biology 2023Quote: ... The peptides were desalted and fractionated using 200 µl Pierce C18 column tips (Merck Millipore).
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were then desalted using styrene divinylbenzene-reverse phase sulfonate Stage-tips (Merck, Darmstadt, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were blocked with 5% non-fat milk in PBS with 0.05% Tween-20 (Merck; blocking buffer) and incubated with primary antibodies (Table S1 ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were blocked with 5% non-fat milk in PBS with 0.05% Tween-20 (Merck; blocking buffer) and incubated with primary antibodies (Supplemental Table 2 ...
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bioRxiv - Neuroscience 2020Quote: ... Parasagittal sections were treated with blocking solution containing 1% normal donkey serum (Merck Millipore, Burlington, MA, USA) and 0.2% TX-100 (Nacalai Tesque ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were then incubated with 10 ml blocking buffer (5% skim milk powder (Merck Millipore, 115363) in TBST ...
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bioRxiv - Microbiology 2023Quote: ... After blocking and washing the slides with PBS with 0.05% TWEEN® 20 (Merck-Millipore, Burlington, USA), plasma from bloodclots or serum was diluted 1:32 (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
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bioRxiv - Neuroscience 2020Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... The digested peptides were further filtered with Amicon ultra centrifugal filters (10kD MWKO, Merck Millipore, MA), dried under vacuum extensively to remove residual primary amine from reagents ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide fractions were eluted using MeOH buffer containing trifluoroacetic acid (TFA) (Merck Sigma, Cat. No. 302031), FA or ABC ...
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bioRxiv - Immunology 2022Quote: ... The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore, Darmstadt, Germany). The peptide lysates were re-suspended in 15 µl 0.1% formic acid and analysed by nanoliquid chromatography mass spectrometry (UltiMate 3000 RSLCnano ...
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bioRxiv - Biophysics 2022Quote: ... Both recombinant and synthetic lyophilized peptides were dissolved in 6M guanidine thiocyanate (Merck KGaA, Darmstadt, Germany) and incubated overnight at 1250 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were eluted with 50 μl of 100 μg/mL of 3X FLAG tag peptide (Merck) in solubilization buffer followed by acetone precipitation (overnight ...
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bioRxiv - Cell Biology 2023Quote: ... pH 2.0) for endogenous MDH2 IP and incubation with 250 μg/ml 3XFLAG peptide (Merck F3290) in the case of FLAG IP ...
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bioRxiv - Bioengineering 2020Quote: ... Following PBS washes (5 min x3) and overnight incubation in blocking buffer (5% bovine serum albumin (Merck Millipore) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Fibers were washed 3×10 min in PBS and incubated in blocking buffer (1% bovine serum albumin (Merck), 5% goat serum (16210-064 ...
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bioRxiv - Developmental Biology 2022Quote: ... cryo-sections were permeabilized and blocked with blocking solution (5% BSA containing 0.3% Triton X-100 (Merck, 93420)) for 1-2 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then washed through three 70μl drops of PBST before being transferred to 50μl drops of blocking 3% BSA (Merck) in PBST for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... anti-DNP IgE sensitized BMMCs were pre-incubated with inhibitors or blocking antibody: 50 µM Y-27632 (Merck), 50 µM blebbistatin (Merck) ...
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bioRxiv - Molecular Biology 2024Quote: ... The sections were treated with blocking buffer (TEM-BB) composed of 1% fish skin gelatin (Sigma-Aldrich / Merck) dissolved in 0.1M HEPES for 1 hour ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Labelled peptides were separated on Chromolith Caprod RP-18e HR capillary column (150 × 0.1 mm; Merck Millipore) using linear gradient of buffer B (98% ACN and 0.05% TFA in water ...
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bioRxiv - Cell Biology 2020Quote: ... and proteins were eluted using 100 µl of 150 ng/µl 3× FLAG Peptide (F4799, Merck KGaA) or HA peptide (HY-P0239 ...
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bioRxiv - Microbiology 2022Quote: ... 100 µg of the dried peptides were dissolved in 90 µl of 0.2 M EPPS buffer (MERCK)/10% acetonitrile ...
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bioRxiv - Pathology 2019Quote: ... The extracted peptides were purified using C18 Zip-TIP pipette tips according to the manufacturer’s instructions (Merck Chemicals ...
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bioRxiv - Biochemistry 2019Quote: Crude peptides were purified by reverse-phase high-performance liquid chromatography using a Chromolith Prep column (Merck) on a Prominence LC-20AP system (Shimadzu ...
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bioRxiv - Biochemistry 2019Quote: Peptides were spotted at 40 µg per origin and dried on thin-layer cellulose plates from Merck. Electrophoresis was run in 1% ammonium carbonate pH 8.9 at constant 1 kV for 25 min 40,41 ...
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bioRxiv - Immunology 2020Quote: ... MHC–peptide complexes were eluted by repeated addition of 0.2% trifluoroacetic acid (TFA, Merck, Whitehouse Station, NJ). Peptides were purified by ultrafiltration using centrifugal filter units (Amicon ...
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bioRxiv - Biochemistry 2023Quote: ... the peptides were desalted and intact glycopeptides were enriched by ZIC-HILIC beads (Merck, Product no. 1.50458.0001) according to our previous study[25].
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bioRxiv - Cell Biology 2021Quote: ... The floated sections were rinsed briefly with 100 mM maleate buffer (pH 7.5) and blocked for 30 minutes against non-specific antibody binding with 2% blocking reagent (Merck) in maleate buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were then permeabilized in blocking solution containing 5% normal goat serum (NGS, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in 0.5% Triton for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated for 24 hours in blocking buffer containing 10% (vol/vol) normal donkey serum (NDS) (S30-100ML, Merck), 0.5% (vol/vol ...
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bioRxiv - Cell Biology 2022Quote: ... The buffers used during the whole procedure had fish gelatine 1.6% (v/v) as a blocking agent (G7765, Merck). The primary antibodies used were anti-NR1D1 (ab174309 ...
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bioRxiv - Neuroscience 2023Quote: ... in samples where biotin-streptavidin amplification was used before blocking with 10% chemiblocker (Merck Millipore, Billerica, MA, USA, #2170) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... All primary and secondary antibodies were diluted in a blocking solution consisting of PBS and 0.2% (v/v) bovine serum albumin (BSA, Merck). Cells were first washed with ice-cold PBS and incubated for 5 min in a blocking solution on ice ...
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bioRxiv - Neuroscience 2024Quote: Whole iPSC-brain organoids were permeabilized with 0.5% Triton X-100 in blocking solution with 2% Bovine Serum Albumin (BSA; Merck) in PBS for 3 hours at RT ...
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bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
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bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...