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Citations for Merck :
101 - 150 of 172 citations for Rubella Virus Nucleoprotein strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: For each experiment the strains were subcultured on Sabouraud dextrose agar medium (SDA; Merck, Darmstadt, Germany) for 48 h at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmid was used to transform Escherichia coli BL21 Rosetta2 (DE3) expression strain (Merck, Darmstadt, Germany), grown to exponential phase in 1 L lysogeny broth (LB ...
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bioRxiv - Immunology 2023Quote: ... Diphtheriae strain was incubated at 37°C in a shaking incubator in BHI broth (Merck, 53286) and was grown to an OD600 of 0.4 to 0.6 ...
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bioRxiv - Microbiology 2024Quote: ... Single colonies of the propagating strains were inoculated into LB medium (Lysogeny Broth, Merck, Darmstadt, Germany) and incubated at 37°C shaking at 170 rpm until reaching the exponential phase (approximately five hours) ...
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bioRxiv - Microbiology 2021Quote: ... One mL of virus stock and 9 mL of MEM-E supplemented with 1% pyruvate (Merck KGaA, Darmstadt, Germany) were added to a conical tube with 16 × 106 SK-6 cells and shaken for 30 minutes at 104 rpm and 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 293T or 293GP virus producing cells were transfected using the GeneJuice reagent according to the manufacturer’s recommendations (Merck Millipore). Two days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... One milliliter of the virus supernatants was directly overlaid on the cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates with confluent cell layers were fixed and virus was inactivated by immersion in 100% methanol (MERCK) for 20 min ...
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bioRxiv - Genomics 2023Quote: ... The transduction was performed by adding the virus particle directly onto the cells supplemented with 6μg/mL polybrene (Merck). The cells were refreshed 24h after transduction and the antibiotics (puromycin/blasticidin ...
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bioRxiv - Molecular Biology 2021Quote: ... Two microliters of each sample was loaded on cellulose F TLC plates (20×20 cm; Merck) and run in a solvent system of isobutyric acid:0.5 M NH4OH (5:3 ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µl were spotted onto a thin layer chromatography (TLC) plate (PEI Cellulose F; Merck Millipore) pre-spotted with 2 µl of nucleotide mix containing AMP ...
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bioRxiv - Genetics 2020Quote: ... which was performed by intramuscular injection with rhFSH (recombinant human follitropin alpha, GONAL-F, Merck Serono) for 8 days ...
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bioRxiv - Biochemistry 2021Quote: ... All reactions were monitored by thin-layer chromatography (TLC, Merck silica gel 60 F-254 plates). The plates were stained either with p-anisaldehyde (2.5% p-anisaldehyde ...
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bioRxiv - Bioengineering 2022Quote: ... The live/dead cells were stained with the Calcein-AM/PI Kit (Merck, 04511-1KT-F) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with filipin complex (Merck, cat #F-9765; 0.15 mg/ML in D-PBS) for 2 h and after several washes in D-PBS were visualized by fluorescence microscopy ...
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bioRxiv - Biochemistry 2023Quote: ... Analytical TLC were performed using prepared plates of silica gel (Merck 60 F 254 on aluminum) and then revealed with UV lights ...
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bioRxiv - Systems Biology 2020Quote: ... albicans strain SC5314 (ATCC MYA-2876) were cultivated on Tryptic Soy Agar (TSA) ReadyPlatesTM (Merck, Darmstadt, Germany) at 37°C overnight ...
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bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
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bioRxiv - Microbiology 2023Quote: ... The strains were first cultivated in separate pure cultures overnight on Tryptic soy agar (TSA; Merck, Germany) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... coli RIL strains carrying the appropriate plasmid were grown in 1 l Luria-broth (LB) (Merck Millipore) supplemented with kanamycin (100 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... the pUL21 gene was amplified from virus stock by PCR with the oligonucleotide primers 5′-ATGGAGCTTAGCTACGCCAC-3′ and 5′-TTTATTGGGGTCTTTTACACAGACTGTC-3′ using KOD polymerase (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... For detection of particle incorporation virus supernatant was further concentrated by centrifugation at 4°C for 2h at 21000xg on 5% Optiprep (Merck), the supernatant was removed and pellet was resuspended and subjected to Western Blot analysis ...
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bioRxiv - Microbiology 2022Quote: ... Ten μl of the supernatant fractions were subsequently spotted on PEI-cellulose F TLC plates (Merck Millipore) and nucleotides separated using a 1.5 M KH2PO4 ...
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bioRxiv - Microbiology 2022Quote: ... ModQ-oE-F and ModQ-oE-R (Supplementary Table S3) with KOD Hot- start DNA polymerase (Merck) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... F-actin was then mixed with a fivefold molar excess of NHS-XX-Biotin (Merck KGaA, Germany) and incubated in the dark for 15 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat. No 71403). Protein expression was always performed using freshly transformed chemically competent E ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: Extraction of RNA from cell lines/strains was carried out using TRI Reagent (Merck Life Sciences, Dorset UK) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... One strain tube thawed rapidly at 37 °C and cultured in 10 ml Brain heart infusion (BHI - Merck) at 37 °C over 24 hours ...
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bioRxiv - Genomics 2021Quote: ... rosea strains was inoculated at edge of a 9-cm-diameter potato dextrose agar (PDA; Merck, Kenilworth, NJ) Petri plate covered with a durapore membrane filter (Merck ...
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bioRxiv - Microbiology 2022Quote: ... was applied onto the dried treatment (10mL of virus was concentrated using centrifugation through Amicon® Ultra-15 Centrifugal Filter Units (Merck). After 5 minutes at room temperature 150μl of DMEM (high glucose ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... One microliter of each sample was then loaded on a thin-layer chromatography plate (PEI cellulose F, Merck) and resolved with KH2PO4 0,75M buffer ...
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bioRxiv - Neuroscience 2020Quote: ... individual COs were transferred to an Eppendorf tube containing CMM medium with 1:100 of virus containing medium and 5µg/ml Polybrene (Merck; TR-1003-6) and incubated over-night ...
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bioRxiv - Molecular Biology 2021Quote: hEnSC were seeded at a confluency of 105 cells/well in 6 well plates and 1.5 ml of concentrated virus added to each well in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... hTERT cells were transduced with 1.5 ml of concentrated pMY-SUZ12-FLAG-IRES-bls virus in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium containing the virus was collected 48 hours post-transfection and filtered through a 0.45 µm polyethersulfone (PES) filter (Merck Millipore, Billerica, MA).
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bioRxiv - Developmental Biology 2023Quote: ... F1 generation of 8-week female hybrid mice (generated via C57BL6 female and CBA/W strain crosses) were intraperitoneally injected with 7.5IU of PMSG (pregnant mare serum gonadotrophin; Merck) and reinjected after 48 hours 7.5IU hCG (human chorionic gonadotrophic hormone ...
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bioRxiv - Plant Biology 2024Quote: A pTYB2-based vector for the expression of PSM polypeptides and a pCDF-based vector that contained heme oxygenase 1 and phytochromobilin synthase genes for the synthesis of PΦB (Muramoto et al., 1999; Kohchi et al., 2001) were transformed into the Escherichia coli Rosetta 2 strain (Merck). E ...
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bioRxiv - Microbiology 2024Quote: ... cerevisiae strains was investigated in SC -Ura medium supplemented with 1.5 ppm prothioconazole dissolved in 50 % DMSO (Merck, NJ) and measuring the OD600 in SpectraMax Gemini™ XPS/EM microplate reader (Molecular Devices ...
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bioRxiv - Neuroscience 2024Quote: Thin-layer-chromatography (TLC) was performed on 0.25 mm Silica-Gel 60 F plates with a 254nm fluorescence indicator from MERCK. The substance detection took place by light with 254nm and 360nm wavelengths ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus of pooled sgRNA phosphatase library titer was estimated by mixing the cells with the serially diluted virus and measuring GFP% on day three post-infection using a Guava® easyCyte™ Flow Cytometer (Merck Millipore). The cells were then infected with the volume of virus estimated to result in a 30–40% infection rate to increase the probability of a single sgRNA introduction event per cell ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells (MDA, A549 and HCT) were then infected with the produced virus using 2μg/ml polybrene (Merck, Darmstadt, Germany, #TR-1003-G). Stably transduced cells were either selected by flow cytometry using BD FACSAria III cell sorter or 1μg/ml puromycin (InvivoGen ...
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bioRxiv - Microbiology 2022Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 µg/mL 2,6- Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Microbiology 2022Quote: ... ljungdahlii strain and its derivatives was carried out in 125 mL serum bottles (Sigma-Aldrich, Merck Life Science, Gillingham, UK) with a working volume of 30 mL ...
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bioRxiv - Microbiology 2023Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 μg/mL 2,6-Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Biochemistry 2023Quote: ... equivalent masses of total lipid fraction from each strain were spotted onto a high-performance TLC (HPTLC) silica gel 60 plate (Merck) with a Camag ATS4 apparatus ...
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bioRxiv - Microbiology 2019Quote: ... nucleotides in a 0.5 μl reaction mixture were separated via thin layer chromatography using TLC PEI Cellulose F plates (Merck Millipore) and 1 M LiCl as solvent ...
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bioRxiv - Bioengineering 2021Quote: ... The LCLs were maintained under aseptic conditions in a 1:1 mixture of Dulbecco’s modified Eagle medium and nutrient mixture F-12 (DMEM/F12; DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were stopped at each time point using 4 N formic acid and analyzed by PEI-Cellulose-F TLC (Merck) developed in 0.4 M potassium phosphate buffer (pH 3.4) ...
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bioRxiv - Microbiology 2020Quote: ... Purified Vag8 was concentrated and the buffer was exchanged to buffer F (50 mM Tris-HCl pH 8.0, 150 mM NaCl) by ultrafiltration (Amicon Ultra, Merck-Millipore).