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Citations for Merck :
101 - 150 of 1107 citations for Recombinant Yellow Fluorescent Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Immunology 2022Quote: ... Animals were randomly assigned to be treated with either 170,000 IU/kg human recombinant interferon alpha-2a (Merck), or BSA/saline (0.9% NaCl ...
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bioRxiv - Bioengineering 2021Quote: INS-1 cells were stained with red fluorescent membrane-inserting dye PKH-26 (Lot# SLBW0232, Merck, Darmstadt, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: ... Total proteins from the supernatant were quantified using a Bradford Protein Assay Kit (Merck) and subjected to electrophoresis on 15% SDS–PAGE gels ...
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
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bioRxiv - Microbiology 2023Quote: ... protein A sepharose beads (Merck) were incubated with the cleared supernatant for an hour to remove non-specific binding ...
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bioRxiv - Neuroscience 2019Quote: ... the rats were treated with either 170,000 IU/kg IntronA (human recombinant interferon-alpha 2b, Merck Sharp & Dohme Limited), or saline (0.9% NaCl ...
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bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
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bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were stirred using Teflon-coated magnetic stirrer bars and monitored by thin layer silica gel chromatography (TLC) using 0.25 mm silica gel 60F plates with fluorescent indicator from Merck. Plates were visualized under UV or treated by KMnO4 stain with gentle heating ...
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bioRxiv - Microbiology 2021Quote: ... Fluorescent profiles were recorded using a Guava easyCyte flow cytometer and analyzed with InCyte software (Merck Millipore, Guyancourt, France).
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bioRxiv - Microbiology 2021Quote: ... Parasitemia was determined daily following tail bleeds and assayed by automated fluorescent cell counting with a Muse cytometer (Merck-Millipore ...
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bioRxiv - Microbiology 2023Quote: ... Parasitemia was determined daily following tail bleeds and assayed by automated fluorescent cell counting with a Muse cytometer (Merck-Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Protein transfer was done using the Bio-Rad wet-blotting system at 500 mA for 1 h on 100% methanol-activated low-fluorescent Immobilon-FL membranes (0.45 μm pore size, Merck). The blot transfer buffer consisted of 25 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Calibration curves were acquired using defined amounts of the fluorescent product 4-methylumbelliferone sodium salt (#M1508, Sigma-Aldrich/Merck). Fluorescence of 4-methylumbelliferone was normalized to crystal violet intensity.
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... protein lysates were cleared by centrifugation and protein concentration was measured using Coomassie reagent (Merck). Denatured ...
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (both from Merck). After 24 h of CHIR99021 stimulation ...
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bioRxiv - Biophysics 2021Quote: ... This mix included 98% unlabeled recombinant human E254N tubulin diluted in assay buffer containing oxygen scavengers (180 mg/ml catalase (C40, Merck) and 752 mg/ml glucose oxidase (22778.01 ...
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bioRxiv - Cell Biology 2023Quote: ... 2020) containing 300 ng GST-tagged MP-GAP fragments and 200 ng recombinant human Aurora A kinase (Merck, 14-511), 1mCi of [gamma-P32]ATP (Hartmann Analytic ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... The dose of COS (75-400 IU daily of recombinant follicle stimulating hormone (Gonal-F Merck or hMG Menopur, Ferring) was individualized to patient’s age ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... Ovarian follicle growth tracking was performed by scheduled transvaginal ultrasound examinations that lead to the planning timepoint for oocyte maturation triggered by administration of recombinant hCG (250 microgr SC Ovitrelle; Merck). Oocyte retrieval was carried out by transvaginal ultrasographically-guided follicular puncture 37 hours later.
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bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards were obtained from Merck.
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed in recombinant tau-lysis buffer (1 mM benzamidine, 1 mM PMSF, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail mix (Merck), 14 mM b-mercaptoethanol ...
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bioRxiv - Developmental Biology 2023Quote: ... healthy female cynomolgus monkeys were subjected to follicular stimulation by intramuscular injection of 20 IU of recombinant human follitropin alpha (rhFSH, Gonal F, Merck Serono) for 8 days ...
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hrs the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore). The HeLa cells stably expressing mouse Cx26 were originally obtained from Dr K ...
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bioRxiv - Neuroscience 2023Quote: ... we applied with unlabeled (S)-PhPrM and (S)-PhPr solutions to the origin point on a reversed-phase plate coated with fluorescent indicator (RP-18 F254S, Merck Millipore). The developed spots were detected with ultraviolet light (254 nm).
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bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
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bioRxiv - Genomics 2020Quote: ... The protein solutions were subsequently desalted and protein amount was estimated using Direct Detect cards (Merck Millipore, Darmstadt). All samples were freeze dried before further processing ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... urinary human menopausal gonadotropin (hMG) (HMG-lepori 75 UI, Angelini Farmacéutica, S.A, Spain) and recombinant hCG (Ovitrelle 250 micrograms, Merck Serono Europe Limited, Spain) or luprorelin acetate (Procrin 1 mg/0,2 ml ...
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bioRxiv - Neuroscience 2021Quote: ... the SH-SY5Y cells were collected and the ROS levels were detected using the fluorescent dye dihydroethidium (DHE) (Sigma-Aldrich; Merck KGaA). Cells were incubated with 5 µM DHE dye for 20 min at 37°C in the dark and then the fluorescence intensity was measured by flow cytometry in the PE channel ...
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bioRxiv - Plant Biology 2023Quote: ... Sections were rinsed 6 times in MTSB and incubated with 1 µg/mL Calcofluor white (Fluorescent Brightener 28 disodium salt solution, Merck, in MTSB). After rinsing ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... and eSpCas9-GFP protein (#ECAS9GFPPR, Merck KGaA) were introduced into MCF7 and T47D cells using Viromer® CRISPR (Lipocalyx GmbH ...
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bioRxiv - Evolutionary Biology 2020Quote: ... protein G-magnetic beads (Merck Millipore, LSKMAGG10) were used instead ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were loaded to a filter (Merck-Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...
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bioRxiv - Neuroscience 2019Quote: ... in combination with cas9 nuclease protein (Merck) was used for gene editing ...
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bioRxiv - Cell Biology 2021Quote: ... in combination with cas9 nuclease protein (Merck) was used for gene editing ...
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bioRxiv - Neuroscience 2021Quote: ... using myelin basic protein primary antibody (MERCK-Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... MWCO 10 kDA protein concentrator (Merck Millipore) to the final concentration of 9-10 mg/ml.
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred onto PVDF membranes (Merck). The membranes were blocked with 5% defatted milk before blotted with primary antibodies and secondary antibodies [anti-mouse IgG-HRP (at 1:2,000 dilution) ...