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Citations for Merck :
101 - 150 of 1545 citations for Recombinant Mouse Angiotensinogen serpin peptidase inhibitor clade A member 8 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... pSmad1/5/8 (Merck, AB3848-I, 1/200), Ki67 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... 8 µg/mL D-pantothenic acid (Merck, P5155), 1 µM rosiglitazone (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Mad1 clone BB3-8 (1:1000, Merck), anti-ZW10 (Rabbit ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with 8 mg/mL Polybrene Reagent (Merck) for 1h ...
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bioRxiv - Cell Biology 2023Quote: ... 1× protease inhibitor cocktail (cOmplete, EDTA-free protease inhibitor cocktail; Merck)] ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Biophysics 2023Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosetta 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 µg/ml kanamycin and 34 µg/ml chloramphenicol ...
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bioRxiv - Biophysics 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Molecular Biology 2023Quote: ... or HIS-Select Nickel Affinity Gel in a gravity-flow column (Merck, Darmstadt, Germany), followed by washing with binding buffer with and without 1.3% Triton X-100 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... the GFP-Trap bead bound fraction was incubated with recombinant TEV (Merck) overnight at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... 100 μl of 10 μg/ml recombinant protein G (Merck, Darmstadt, Germany) was added and incubated for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... then 1,000 IU recombinant human chorionic gonadotropin alpha (rhCG, OVIDREL, Merck Serono) was injected on day 9 ...
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bioRxiv - Plant Biology 2019Quote: ... Protease inhibitor mix (Merck), and 50 μM MG-132 (Cayman Chemical) ...
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bioRxiv - Cell Biology 2022Quote: ... phosSTOP phosphatase inhibitors (Merck)) ...
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bioRxiv - Cell Biology 2022Quote: ... cOmplete protease inhibitors (Merck) and incubated for 20 minutes on ice ...
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bioRxiv - Cell Biology 2022Quote: ... cOmplete protease inhibitors (Merck), phosSTOP phosphatase inhibitors (Merck)) ...
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bioRxiv - Biochemistry 2021Quote: ... protease inhibitor cocktail (Merck)] ...
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bioRxiv - Cell Biology 2021Quote: ... and phosphatase inhibitors (Merck), 50 μl of samples were collected (total cell lysates) ...
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bioRxiv - Cell Biology 2022Quote: ... protease inhibitor cocktail (Merck), 1mM DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... and phosphatase inhibitor (Merck). After 10 cycles of sonication using Bioruptor Pico (Diagenode) ...
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bioRxiv - Cell Biology 2024Quote: ... protease inhibitor cocktail (Merck), 1 mM DTT ...
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bioRxiv - Immunology 2024Quote: ... protease inhibitor cocktail (Merck) was used according to manufacturer instructions or pericytes were directly exposed to recombinant m-MCP6 (R&D systems ...
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bioRxiv - Immunology 2020Quote: ... two STAT-3 inhibitors (STAT-3 inhibitor III (WP-1066) and STAT-3 inhibitor XIII (C-188-9) were tested (Merck, Sigma) and WP-1066 was used for infection studies at a final concentration of 12 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’ cyclic mono-phosphate (8-Br-cAMP, Merck B5386) and 1 μM medroxyprogesterone acetate (MPA ...
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bioRxiv - Systems Biology 2022Quote: ... or 8 M Urea (Merck Sigma, Cat. No. U5378) and phosphatase/protease inhibitors ...
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bioRxiv - Bioengineering 2022Quote: ... in 150 mM NaHCO3 buffer (Merck, #144-55-8) with a pH of 11 ...
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bioRxiv - Developmental Biology 2023Quote: ... 2.25ml of BSA solution 35% (Merck 9048-46-8).
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were denatured with 8 M urea (Merck, U0631) in 0.1 M Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5 mM TCEP) with protease inhibitors (Merck Protease inhibitor cocktail III, 1:500). Cells were disrupted by sonication for 15 mins 10sec on 5sec off ...
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bioRxiv - Biochemistry 2022Quote: ... treated with protease inhibitors (cOmplete™ EDTA-free Protease Inhibitor Cocktail (Merck, 04693132001) or Pefabloc® SC (CarlRoth ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with protease inhibitor and phosphatase inhibitor cocktail (Merck Millipore, Burlington, MA, USA) on ice ...
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bioRxiv - Biochemistry 2022Quote: ... mouse anti-mouse FAP (Merck, MABC1145), mouse anti-CAR (Millipore ...
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bioRxiv - Genetics 2021Quote: Before the ovaries were stimulated with recombinant FSH (Gonal-F, Merck Serono, Switzerland), downregulation was performed using a GnRH agonist (Decapeptyl ...
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bioRxiv - Zoology 2020Quote: ... Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck) in TE buffer (10 mM Tris-HCI ...
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bioRxiv - Bioengineering 2021Quote: ... recombinant human platelet derived growth factor (hPDGF-AB, 20 ng/ml, Merck, GF106), recombinant human oncostatin M (hOSM ...
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bioRxiv - Cell Biology 2022Quote: All experiments were performed using the BDNF mature recombinant (GF029; Merck Millipore, USA) or the C-ter truncated form of BDNF (BDNFΔC-ter) ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant FGF-2 (10 ng/ml; Merck Millipore, Burlington, MA, USA) as mitogens ...
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bioRxiv - Immunology 2023Quote: ... Mo were primed with 200 U/mL human recombinant IFNα2β (Intron A; Merck) for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D ...
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... The expression of S-tagged NrdA was confirmed by purification using S-protein agarose (Merck Millipore, Darmstadt, Germany) as previously described (11 ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of His6-tagged proteins from the soluble fraction was achieved using Ni-NTA His.Bind® Resin (Merck), a peristaltic pump and a series of column washes with buffers of increasing imidazole content ...
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bioRxiv - Evolutionary Biology 2020Quote: ... F2 FLAG-tagged TBP::NveRLR::mCherry and wild-type zygotes were treated with 3% L-Cysteine (Merck Millipore), washed and snap frozen in liquid nitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... The filtered supernatant was loaded onto a column packed with cOmpleteTM His-tag purification resin (Merck). His6-proteins were eluted with 4x 2 bead volumes of elution buffer (20 mM Na HEPES ...
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bioRxiv - Immunology 2023Quote: ... PDGFR inhibitor CP-868596/Crenolanib (Selleckchem) and the metalloproteinase inhibitor GM6001/ Galardin (Merck Millipore) were used at the concentration of 1 μM and 100 µM respectively and refreshed as described above ...