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Citations for Merck :
101 - 150 of 3054 citations for Neutrophil cytosol factor 1 NCF1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: The rat INS-1 832/3 cell line (insulinoma cell line stably transfected with human insulin; hereinafter INS-1) was obtained from Merck. A HUVEC (human umbilical vein endothelial cell ...
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bioRxiv - Cell Biology 2019Quote: ... They were coated for 1 hour with 50 μg/mL human plasma fibronectin (Merck Millipore) diluted in PBS and washed three times with PBS.
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bioRxiv - Molecular Biology 2021Quote: ... and goat anti-Human IgG (Fc specific)-Cy3 (1:1000 dilution in binding buffer; Merck). Finally ...
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bioRxiv - Microbiology 2021Quote: ... Human HDL (Merck Millipore) was incubated with NS1 for 1 hour at 37°C prior to the DSC experiments ...
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bioRxiv - Immunology 2021Quote: Native human insulin (Merck) was pre-diluted in water to 1 mg/mL ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Cell Biology 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Biophysics 2023Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosetta 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 µg/ml kanamycin and 34 µg/ml chloramphenicol ...
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bioRxiv - Biophysics 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Molecular Biology 2023Quote: ... or HIS-Select Nickel Affinity Gel in a gravity-flow column (Merck, Darmstadt, Germany), followed by washing with binding buffer with and without 1.3% Triton X-100 ...
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bioRxiv - Genomics 2022Quote: ... the media was supplemented with 1000 units/ml leukemia inhibitory factor (LIF) (Merck Millipore), 1µM PD0325901 ...
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bioRxiv - Microbiology 2023Quote: ... and mouse anti-human TMPRSS2 (monoclonal, clone P5H9-A3, cat num: MABF2158, 1:500, MERCK, USA) for a minimum of 2 h at RT ...
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bioRxiv - Cell Biology 2019Quote: Human fibronectin was from Merck Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... 5.06% fibrinogen (human plasma; Merck), 3U/mL thrombin (Stago BNL ...
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bioRxiv - Microbiology 2022Quote: ... Plasma-purified human thrombin (Merck) was then added to reactions as required from a 1 IU / μL stock ...
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bioRxiv - Microbiology 2022Quote: ... The dilution factor was ~15 for each round and the centrifugal concentrator (Merck Millipore Ltd.) had a molecular weight cut-off at 3 kDa ...
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bioRxiv - Developmental Biology 2023Quote: ... medium was changed to N2B27 supplemented with growth factors and small molecule inhibitors: CHIR99201 (Merck), murine BMP4 (PeproTech 315-27) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng/ml cholera toxin and 10 ng/ml epidermal growth factor (Merck Sigma-Aldrich). 4-Hydroxytamoxifen (4OHT ...
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bioRxiv - Plant Biology 2022Quote: ... The filtered supernatant was loaded onto a column packed with cOmpleteTM His-tag purification resin (Merck). His6-proteins were eluted with 4x 2 bead volumes of elution buffer (20 mM Na HEPES ...
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bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
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bioRxiv - Cancer Biology 2021Quote: ... however without growth factors but supplemented with glucose (#G7021-1KG, Sigma, part of Merck, Darmstadt, German) and cells seeded into a precoated uClear® 96-well plate ...
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bioRxiv - Developmental Biology 2022Quote: ... EBs were not embedded and patterning factors including 100 nm SAG (Merck-Millipore, cat. no. US1566660) and 2.5 µM IWP2 (Sigma-Aldrich ...
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: ... For mESCs the medium was supplemented with 1000 units/ml leukemia inhibitory factor (LIF) (Merck Millipore), 1 µM PD0325901 ...
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bioRxiv - Immunology 2022Quote: ... Human heat-aggregated IgG immune complexes (ICs) were prepared by heating human IgG1 isotype control (Merck) for 20 min at 63°C.
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bioRxiv - Cell Biology 2021Quote: Human hTERT RPE-1 cells (ATCC, CRL-4000) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM/F12, Merck) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Cell Biology 2024Quote: ... cover slips were incubated with the primary antibody (SV40 T antigen mouse-anti-human, Merck-Millipore, 1:50) overnight at 4 °C in a humidifier chamber ...
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bioRxiv - Bioengineering 2019Quote: ... human fibronectin (Merck-Millipore, Watford, UK) was premixed with Laponite gels to a final concentration of 50 µg/mL ...
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bioRxiv - Microbiology 2019Quote: ... Binding of human FH (Merck Millipore) was detected with Alexa-Fluor-488 labeled human FH ...
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bioRxiv - Immunology 2022Quote: ... Purified C1q from human serum (Merck) was used as a control.
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bioRxiv - Systems Biology 2020Quote: ... anti-human MLKL (Merck Millipore, MABC604), phospho anti-human RPB1 S2 (Millipore ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... Human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 μg/μl for a 1h incubation at 37°C ...
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bioRxiv - Immunology 2023Quote: Human IFNβ (IF014, Merck, KGaA, Darmstadt), IFNα2a (11100-1 ...
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bioRxiv - Immunology 2024Quote: ... Human MC lines HMC-1.1 (Merck) and HMC-1.2 (Merck ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 μg/ml human insulin (Merck), and 50 μM hydrocortisone (Cayman ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then incubated with 2 mL pre-washed HIS-Select Cobalt Affinity Gel (Merck, UK) with rotation for an hour ...
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bioRxiv - Cell Biology 2021Quote: ... 1000 units/ml of recombinant Leukemia Inhibitory Factor (LIF, Interchim, #8V6280) and 0.1% β-mercaptoethanol (Merck, #M6250) on gelatin-coated plates ...
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bioRxiv - Neuroscience 2023Quote: ... NBP1-92693AF647) and the oligodendrocyte transcription factor OLIG2 (Anti-OLIG2 clone 211F1.1 mouse mAb, Merck Millipore, MABN50A4). Samples were kept on ice throughout the isolation and staining procedure ...
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bioRxiv - Physiology 2019Quote: ... The hPCLS were treated with 10ng/ml recombinant human TGFß 1 protein (R&DSystems#240-B-010) and 25µM (in 750µl of DMEM) of GM6001 (Merck#CC1010). Lived dead staining was carried out using calcein-am/ ethidium homodimer kit (ThermoFisher#L3224 ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: A549 (human epithelial lung cell line) and EA.hy926 (human endothelial somatic cell line) cells were bought from Merck company and ATCC ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... 10 μg/ml human holo-Transferrin (Merck), 12.5 mg/ml AlbuMAX I (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... and human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 µg/µl for a 1h incubation at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... human recombinant insulin (100 µg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1 ...