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Citations for Merck :
101 - 139 of 139 citations for Hydroxy PEG4 t butly ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 times with PBS-T for 5 min and incubated with mouse-HRP (A4416; Merck) and rabbit-HRP (GENA9640V ...
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bioRxiv - Immunology 2024Quote: ... plates were washed in PBS/T and incubated for 1 h with goat anti-mouse IgG-AP (Merck). Plates were then developed as per the mAb ELISA ...
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bioRxiv - Cell Biology 2024Quote: ... cover slips were incubated with the primary antibody (SV40 T antigen mouse-anti-human, Merck-Millipore, 1:50) overnight at 4 °C in a humidifier chamber ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked in 1XPBS + 0,1% Tween (1XPBS-T) with non-fat dry milk (5%, Merck Millipore) for 30 minutes at RT and then incubated for 3 hours at RT with a primary antibody resuspended in 1XPBS-T with 5% milk ...
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bioRxiv - Cell Biology 2023Quote: hTERT RPE-1 and HEK293(T) cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM/F12; Merck) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... and then repeatedly washed with PBS-T before incubation in secondary antibodies for GRIN2D (Merck, MAB5578, 1:500) and B-actin (Merck ...
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bioRxiv - Bioengineering 2024Quote: ... the membranes were washed three times for 10 min in PBS-T (PBS with 0.1% Tween-20, Merck). Then ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... the membranes were incubated o/n at 4°C in 10 ml washing buffer (1% [w/v] milk powder in TBS-T) containing a 1:10,000 dilution of anti-pan-ADP-ribose binding reagent MABE1016 (Merck) at 4°C48 ...
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bioRxiv - Cell Biology 2024Quote: ... Blots were washed three times in TBS-T followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck). Following washing in TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were cultured in TexMACS Medium (Miltenyi Biotech) supplemented with 5% heat-inactivated human AB serum (Merck), 100 units/mL penicillin (Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... the HCR protocol involved rehydrating the gastruloids with a descending series of methanol concentrations and finally PBS-T (0.1% Tween, 655204, Merck). Once hydrated ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed thrice with TBS-T and visualized on a LAS4000 Fuji imaging system after incubating with Immobilon Western Chemiluminescent HRP substrate (Merck). The following antibodies were used ...
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bioRxiv - Systems Biology 2020Quote: The constructs containing the TCA cycle enzymes fused to the biotin ligase and a FLAG tag were stably integrated in the Flp-In T-REx HeLa cells using the X-tremeGENE 9 DNA Transfection Reagent (Merck) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with the respective HRP-conjugated secondary antibody in 5% dry milk powder in TBS-T for 1h at room temperature washed three times in TBS-T and imaged on an ECL ChemoCam 3.2 Imager (Intas) using Immobilon Forte Western HRP substrate (Merck Millipore). For pulldown of gH-V5 or gH-V5/ gL-Flag complexes ...
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bioRxiv - Microbiology 2022Quote: ... Hydrolysis of 44 soluble non-chromogenic monoester substrates (Table S2) and T-2 mycotoxin (Merck Life Science S.L.U., Madrid, Spain) was assayed at 37ºC using a pH indicator assay with Phenol Red and monitored at 550 nm (46) ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 3x with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using a Gel-Doc XR+ System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were rinsed with PBS-T and once with deionized water before mounting with FluorSave mounting medium (Merck-Milipore, Germany) and coverslipping ...
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bioRxiv - Neuroscience 2023Quote: ... washed in TBS-T and incubated with horseradish peroxidase–conjugated anti-rabbit (1:2000, #7074P2, Cell Signalling or 1:80000, #A0545, Merck) and anti-mouse (1:2000 ...
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bioRxiv - Genetics 2024Quote: ... Blots were washed thrice with TBS-T and visualized on a LAS4000 Fuji imaging system after incubating with Immobilon Western Chemiluminescent HRP substrate (Merck). The following antibodies were used ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were blocked for 1 hours at 21°C or overnight at 4°C with 5% (w/v) skim milk in PBS-T (PBS tablets; Merck 524650, 0.1% Tween-20; Merck P1379). The membrane was incubated with 1:5000 anti-flagellin antibody (Taguchi et al ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were immunoblotted with an anti-puromycin antibody (MABE343, Merck, 1:2000 dilution in PBS-T with 5% (w/v) BSA ...
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bioRxiv - Microbiology 2024Quote: ... washed three times with PBS-T and stained overnight at 4°C in PBS-T/5% milk containing primary antibodies (anti-phosphorylated STAT1, Cell Signalling Technology and anti-alpha Tubulin, Merck) [1:1000] ...
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bioRxiv - Biochemistry 2021Quote: ... The buffer of the anti-PTP1B antibody was exchanged to PBS-T using a Microcon-30kDa centrifugal filter unit (MRCF0R030, Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Th1 cell differentiation was induced by culturing CD4 T cells with antigen presenting cells treated with 50μg/ml mitomycin C (Merck, Darmstadt, Germany) in the presence of 1μg/ml OVA323-339 (Peprotech ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed 3× five minutes in BSA-T and incubated with an antibody against the FLAG-tag (#F1804 Sigma-Aldrich, now Merck, or #14793 Cell signaling ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were then washed with TBS-T three times for five minutes and exposed to Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) according to product instructions for 30 seconds before being developed ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 0.1% Tween-20 (PBS-T) and blocked using PBS supplemented with 4% w/v skimmed milk powder (PBSM-4%; Merck Millipore) for 1h at room temperature (RT) ...
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bioRxiv - Microbiology 2020Quote: Quantification of Th1/Th2 cytokines in supernatant of splenocytes was performed using mouse high sensitivity T cell magnetic bead panel assay (MHSTCMAG-70K, Merck, Darmstadt, Germany). 5×105 isolated splenocytes were co-cultured with different stimuli in 200 μL RPMI + 10% FBS ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Bioengineering 2023Quote: ... two thirds of the medium was removed from all wells and T cells were treated with 5 ng/mL polybrene (Merck, Darmstadt, Germany). Lentiviral particles (MOI=3 ...
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bioRxiv - Immunology 2024Quote: ... were co-incubated with TCR-T cells (2,000-10,000 cTCR-positive cells/well) in IFN-γ antibody-coated Multiscreen HTS plates (Merck-Millipore, Darmstadt, Germany) overnight (16-20h) ...
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bioRxiv - Cell Biology 2022Quote: ... Testes were washed three times (10 min each) with PBS-T and incubated with anti-H4ac (Merck Millipore #06-598, 1/500 dilution), anti-Histones (Millipore #MABE71 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% BSA TBS-T or 5% skim milk TBS-T and incubated overnight at 4°C with the following primary antibodies diluted in 5 % BSA or 5% skim milk dissolved in TBS-T: pSer293-PDH (1:1,000) (Merck Millipore, Cat. no. ABS204), PDH (1:1,000 ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet containing the lentivirus was resuspended in PBS and Jurkat T cells were transduced in complete medium supplemented with 8 μg/mL polybrene (Merck-Millipore, TR-1003-G) using a spinoculation protocol ...
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bioRxiv - Immunology 2023Quote: Concentrations of cytokines in supernatants of T cell cultures were assessed with the MILLIPLEX MAP Human Th17 magnetic bead panel kit (Merck Millipore, Burlington, MA, USA) and the Luminex® 200™ system according to the guidelines of the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... or 5% (for loading controls) (w/v) non-fat milk powder (NFM; Campina, Amersfoort, Netherlands) in Tris buffer saline with Tween 20 (Merck, Kenilworth, NJ, USA; TBS-T) for 1h at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were blocked for 1 hours at 21°C or overnight at 4°C with 5% (w/v) skim milk in PBS-T (PBS tablets; Merck 524650, 0.1% Tween-20; Merck P1379). The membrane was incubated with 1:5000 anti-flagellin antibody (Taguchi et al ...