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Citations for Merck :
101 - 150 of 1958 citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg of lysate was incubated overnight with 20µl anti-Flag M2 magnetic Dynabeads (Sigma-Aldrich/Merck) under shaking ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were incubated overnight under rotation at 4°C with ANTI-FLAG® M2 Affinity Gel beads (Merck) to pull down FLAG-containing SNAP-GLP-1R ...
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bioRxiv - Biochemistry 2021Quote: ... were incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl/ml of pre-washed Pierce magnetic protein A/G beads (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysates were normalised across samples and were added to 100 μl anti-FLAG M2 affinity gel (Merck) Micro Bio-Spin columns (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... 200 μl of IP buffer and 10 μl of anti-FLAG M2 Affinity Agarose Gel (A2220, Merck Millipore) were added to the 200 μl of lysate ...
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bioRxiv - Microbiology 2023Quote: ... on magnetic rack and then resuspended in 300µL of ChIP buffer and 3µg of anti-Flag antibody (F1804-200UG, Merck) before being placed on wheel for 1h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined and both fractions were incubated with Anti-FLAG® M2 Magnetic Beads (Merck, M8823). Beads were washed once then each sample was split for MS ...
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bioRxiv - Neuroscience 2024Quote: ... Total 2 mg lysate (1 ml) was incubated with 10 µl of anti-FLAG M2 Magnetic Beads (Merck), which was pre-washed twice with IP buffer ...
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bioRxiv - Plant Biology 2021Quote: ... α-FLAG-HRP (A8592, Merck; 1:5,000) and α-rabbit-HRP (A-0545 ...
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bioRxiv - Biochemistry 2023Quote: ... Blots were probed with FLAG (Merck, F3165), ubiquitin (Novus ...
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bioRxiv - Biochemistry 2023Quote: ... mouse monoclonal FLAG-HRP (Sigma-Aldrich (Merck), A8592) ...
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bioRxiv - Plant Biology 2023Quote: ... α-Flag M2 antibody (Merck, F3165, 1:1000), α-pan-ADPr binding reagent (Merck ...
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bioRxiv - Cell Biology 2021Quote: ... Virotrap particles containing protein complexes were purified in a single step using biotinylated anti-FLAG BioM2 antibody (#F9291, Merck) and Dynabeads MyOne Streptavidin T1 Beads (#65601 ...
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bioRxiv - Microbiology 2021Quote: ... The pellets were resuspended in the buffer and incubated with additional 80 µL anti-FLAG M2 affinity gel (Merck) on a rotator overnight at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... 50 μL/well of detection antibody (anti-FLAG-D2) diluted in Erenna Assay buffer (catalog #02-0474-00; Merck) at the final concentration of 1 μL/mL were added to the assay plate and incubated for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated for 1 hour with HRP-conjugated secondary antibodies (1:5000, anti-mouse, Sigma-Aldrich Catalogue No A9044; Flag, or 1:10000, anti-rabbit, Merck, Catalogue No GENA934; LpoA) in TBS-TM ...
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Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic developmentbioRxiv - Microbiology 2020Quote: ... and the remaining supernatant was used for chromatin immunoprecipitation and mixed with magnetic beads (60 μl) coated with anti-FLAG M2 antibody (Merck). Immunoprecipitation was performed overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mg of total protein at a 1 mg/ml concentration was incubated with 40 µl anti-FLAG-M2 affinity resin (Merck) (which had been blocked overnight with 1% BSA and 5 µl/ml ssDNA ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mL of filtered protein extract was mixed with 40μL of anti-FLAG M2 magnetic beads (Merck, formerly Sigma-Aldrich) (equilibrated in GTEN + 0.1 % Tween-20 prior to use ...
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bioRxiv - Cell Biology 2023Quote: ... The pooled fractions were diluted with 2 vols of KPEM buffer to achieve a final concentration of 125 mM KCl and then incubated for 1 hr with 4 mL anti-FLAG monoclonal antibody conjugated resin (M2 agarose, Merck) using rolling ...
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bioRxiv - Molecular Biology 2023Quote: ... the localization was made visible by immunofluorescence staining using an anti-flag antibody (F7425, Sigma Aldrich, Merck, Kenilworth, NJ, USA) in combination with Alexa 546 secondary antibody (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... A sample of the supernatant was collected as “input” and the remaining solutions were either mixed or separately incubated with previously washed Anti-Flag M2 beads (Merck) by tumbling for 150 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The α-FLAG antibody was purchased from Merck and α-GAPDH from Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL of Flag-M2 agarose beads (Merck) were incubated with Flag-tagged FANCJ wild type or AALA mutant (2 μg of each purified recombinant protein ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μL of Flag-M2 agarose beads (Merck) were added to cell extract aliquots containing 1-2 mg of total protein ...
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bioRxiv - Cell Biology 2023Quote: ... which were eluted FLAG® peptide (Merck-SIGMA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... 500 μg/mL FLAG® peptide (Merck; F3290)) were added to the beads ...
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bioRxiv - Biochemistry 2021Quote: FLAG-CAHS1 was constructed using standard genetic engineering techniques with an N-terminal FLAG using a pT7-FLAG-1 vector (Sigma-Aldrich, Merck). The bacterial cells of E ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were clarified by centrifugation and a minimum of 200μg of lysate was incubated with pre-washed EZview™ Red ANTI-FLAG® M2 Affinity Gel (F2426, Merck) at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilised mitochondria were then clarified by centrifugation at 16,000 g for 10 min and incubated with pre-washed anti-Flag® M2 Affinity Gel (Merck; A2220) at 4 °C for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ml of extract was incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl Pierce magnetic protein A/G beads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Cell Biology 2019Quote: ... cleared extracts were incubated ON at +4 °C in an orbital shaker with anti-FLAG sepharose beads (M2 clone, Merck, A2220). Bound complexes were pelleted ...
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bioRxiv - Cell Biology 2019Quote: ... cleared extracts were incubated 2h-6h at +4°C in an orbital shaker with anti-FLAG sepharose beads (M2 clone, Merck, A2220). Bound complexes were pelleted and 5x washed (Lysis buffer with 1M NaCl) ...
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bioRxiv - Genomics 2021Quote: Localization of resulting plasmids was assessed with immunofluorescence staining using an anti-flag antibody (F7425, Sigma Aldrich, Merck, Kenilworth, NJ, USA) in combination with Alexa 546 secondary antibody (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatants were transferred to new tubes and incubated overnight at 4 °C with 15 µl of Anti-FLAG® M2 Magnetic Beads (Merck) with gentle mixing ...
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bioRxiv - Plant Biology 2024Quote: ... and 160 U/mL SUPERase•In™ RNase Inhibitor) and incubated with anti-FLAG® antibody magnetic beads (Merck, Cat# M8823) overnight at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... The magnetic FLAG M2 beads (M8823 Merck Millipore, USA) were equilibrated in the lysis buffer and blocked with 5% BSA ...
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bioRxiv - Microbiology 2023Quote: ... and α-FLAG antibody coupled to agarose beads (Merck) for ChIP-seq of RpoC-FLAG and FLAG-GafZ.
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bioRxiv - Microbiology 2023Quote: ... 1:2,500 dilution of an α-FLAG antibody (Merck), or a 1:5,000 dilution of an α-ParB polyclonal antibody (custom antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Molecular Biology 2020Quote: ... CsCl fractions containing needle complexes and the substrate were pooled and incubated with Anti-FLAG M2 magnetic beads (M8823, Sigma Aldrich/Merck KGaA) prewashed in FR3 buffer (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The cell lysates were centrifuged at 16,000 x g for 10 minutes at 4°C and the supernatants were then incubated overnight at 4°C with anti-Flag M2 affinity gel beads (Merck, Cat.N. A2220). M2 beads were then pelleted and washed three times with lysis buffer containing protease inhibitors ...
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bioRxiv - Bioengineering 2023Quote: ... and mouse monoclonal Flag antibody (clone M2, 1:1000, Merck). The following secondary antibodies were used ...
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bioRxiv - Microbiology 2023Quote: ... α-M2 FLAG antibodies coupled to sepharose beads (Merck, UK) were employed for ChIP-seq of Cada2-FLAG and RpoC-FLAG ...
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bioRxiv - Plant Biology 2021Quote: ... the sections were incubated in 1× PBS containing 1% (1:100 dilution) mouse monoclonal anti-Flag M2 primary antibody (Merck, cat. no. F1804) and 0.1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Cell Biology 2021Quote: ... and were consecutively eluted by competition using FLAG peptide (#F3290, Merck). For immunoblot analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... Cover slips were then incubated with α-FLAG antibody (Merck, F7425) for 1h followed by incubation with goat α-rabbit Alexa Flour 488 (Thermo Fisher ...