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Citations for Merck :
1351 - 1400 of 4341 citations for Tumor Protein Translationally Controlled 1 TPT1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... L1s were then broken up to extract proteins using a Beadbug homogenizer (Merck Life Science) at the highest setting for 90 seconds three times at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were concentrated using 3 kDa MWCO Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore). Protein concentrations were determined using the Proteins and Labels function on a Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometer and subsequently confirmed on SDS-PAGE gel upon Coomassie staining and colorimetric imaging on an Amersham ImageQuant 800 CCD Imager.
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bioRxiv - Molecular Biology 2022Quote: The concentration of protein extracts was determined by BCA assay kit with BSA standards (Merck).
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bioRxiv - Microbiology 2023Quote: ... The proteins were separated via 15% SDS-PAGE and transferred to nitrocellulose membranes (Merck-Millipore). Western blot analyses were then conducted using PilA- ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein bands were visualised using a chemiluminescence substrate (Immobilon Forte, Merck Life Science UK Ltd.) on the ChemiDoc Imaging system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... eluted proteins were then concentrated using amicon ultra-15 centrifugal filters (Cat# UFC901024, Merck Millipore).
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bioRxiv - Plant Biology 2022Quote: ... 100 μg of protein were loaded into FASP filters (Amicon Ultra 30K, 0.5 ml, Merck) and tryptically digested over night following the FASP (Filter-Aided Sample Preparation ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 mins at 60°C with 10 mM dithiothreitol (Sigma, Merck) in 50 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously123 ...
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bioRxiv - Cell Biology 2023Quote: Protein extracts were transferred into an Amicon Ultra Centrifugal filter device (10 kDa MWCO, Merck) for buffer exchange [50 mM Tris-HCL pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 75 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Developmental Biology 2023Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to PolyVinyliDene Fluoride 0.45 µm membranes (Immobilon-Fl, Merck Millipore, Darmstadt, Germany). Membranes were saturated 1 hour with a Tris-Buffered Saline blocking solution (TBS blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to FL-PVDF membranes (cat. No. IPFL00010; Merck Millipore; Massachusetts, USA). The membranes were incubated with ms anti-Y1-R (1:500 ...
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bioRxiv - Physiology 2023Quote: ... The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously107 ...
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bioRxiv - Physiology 2023Quote: The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously23 ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were resolved via SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane (Merck, GE10600023) with a wet electroblotting system (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated using SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-FL, Merck). The membranes were blocked for 30 min with Blocking-One or Blocking-One P (Nacalai Tesque ...
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bioRxiv - Biochemistry 2024Quote: ... Glycerol-free eluted protein was concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (Merck) at 10,000 x g ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was filtered through an Ultrafree-MC centrifugal device with hydrophilic PTFE membrane (Merck) at 10,000 x g ...
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bioRxiv - Molecular Biology 2024Quote: ... and protein complex was eluted in lysis buffer 2 containing 2.5 mM D-desthiobiotin (Merck). Protein tags were removed by overnight cleavage with HRV 3C protease ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was transferred to an Immobilon®-FL PVDF membrane (Merck Millipore, Billerica, MA, USA) and blocked in Licor blocking buffer for 120 minutes (Licor ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein eluates were then desalted and concentrated by ultrafiltration with Amicon Ultra 0.5 10K (Merck Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... In brief supernatant protein concentration was standardised prior to being concentrated using Microcon® (Merck) 10Kda filter columns and centrifugation (14,000g 20 minutes) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were concentrated using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore). Protein and ligand samples were degassed prior to each ITC experiment ...
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bioRxiv - Cell Biology 2024Quote: ... The chromatin (100 μg) was precleared with protein A agarose/Salmon sperm DNA beads (Merck) and 2 % taken as input ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein was extracted using RIPA buffer mixed with protease inhibitor cocktail (Millipore Merck, 539134) and quantified using Qubit® Protein Assay Kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% glutamine and 1% penicillin/streptomycin (Merck). Fascin KD cells were selected and maintained using puromycin ...
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bioRxiv - Neuroscience 2020Quote: ... GluA1 (1:250-1:500; Merck Millipore) and RTP801 (1:100 ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromatin corresponding to 13 μg of DNA was diluted 10-fold in RIPA buffer without SDS complemented with protease inhibitors and incubated overnight at 4°C with 4 μg of H3K9me3 or H3K27me3 antibodies (Frapporti et al., 2019) or with 4 μL of H3K4me3 monoclonal antibody (Merck Millipore #04-745). As input control ...
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bioRxiv - Neuroscience 2024Quote: ... 1-methyl-D-tryptophan (1-DMT; 1-LMT enantiomer, both Merck Life Sciences) for exploration of a possible tryptophan mechanism of immunosuppression ...
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bioRxiv - Molecular Biology 2021Quote: ... CTCF antibody (07-729, Merck Millipore; 5 μL per ChIP) was coupled with Protein G beads (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... or 5 μg rabbit IgG isotype control antibody (Merck-Millipore) for 16 h at 4°C with rotation ...
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bioRxiv - Neuroscience 2021Quote: The acetylated Tubulin antibody (T7451, MERCK/Sigma-Aldrich, Darmstadt, Germany) is a widely used antibody that detects acetylated α-tubulins in several distantly related species (Table 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... secondary antibodies and enhanced chemiluminescent (ECL) reagents (WBLUF0100, Merck Millipore).
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bioRxiv - Molecular Biology 2019Quote: ... or 4 μg of H3K27me3 antibody (17-622, Merck Millipore).
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies used were: rabbit anti-Tyrosine Hydroxylase (Merck-Millipore), 0.1 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-S-100 β subunit antibody (S2532, Merck), rat monoclonal anti-CD68 antibody (ab53444 ...
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bioRxiv - Molecular Biology 2020Quote: ... Other antibodies used are: anti-MYC (Merck Millipore, OP-10), anti-GFP (Santa Cruz ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies (ROR2: Cell Signalling Technologies D3B6F, GAPDH: Merck 6C5) were added and incubated on a roller overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... and immunostained with a mouse anti-NeuN antibody (Merck Millipore). Fluorescence images were acquired with a confocal laser-scanning microscope (TCS SP8 ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation was conducted with anti-H3K27me3 antibody (Merck, 07-449). For REF6-HA binding ChIP ...
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bioRxiv - Biophysics 2021Quote: goat anti-rabbit IgG Atto647N-labeled monoclonal antibody (40839, Merck), dilution ...
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bioRxiv - Biophysics 2021Quote: goat anti-mouse IgG Atto594-labeled monoclonal antibody (76085, Merck), dilution ...
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bioRxiv - Neuroscience 2022Quote: Anti-NFL antibody clone DA2 (Merck Millipore cat. no. MAB1615) was used for immunostaining in all experiments except for that yielding the data shown in Supplementary Fig ...
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bioRxiv - Genetics 2019Quote: ... 8) anti-CREB3L1 mouse monoclonal antibody 44c7 (Merck Millipore, MABE1017) (dilution 1:1000) ...
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bioRxiv - Molecular Biology 2021Quote: ... and immunoblotted with specific antibodies: Histone H4 (Merck, 05-858R), 1:5000 ...
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bioRxiv - Biochemistry 2021Quote: ... and 15 μg anti-puromycin antibody (clone 12D10, Merck Millipore) per IP experiment were mixed in PBS-0.02% Tween 20 (PBS-T ...
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bioRxiv - Genomics 2021Quote: ... anti- FLAG antibody (F7425, Sigma Aldrich, Merck, Kenilworth, NJ, USA) was added to each sample and rotated overnight at 4 °C ...