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Citations for Merck :
1351 - 1400 of 1415 citations for Recombinant Human RELT Protein hIgG His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: For phosphorylated proteins RIPA lysis buffer was further supplemented with PIC and 1X phosphatase inhibitor cocktail (Merck Millipore, cat no. 524629-1ML). A cocktail of phosphatase inhibitors (B-Glycerolphosphat (25mM) ...
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bioRxiv - Biochemistry 2021Quote: RNA immunoprecipitation (RIP) assays were conducted using the EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck Chemicals (Shanghai Co., Ltd). The anti-YTHDC1 antibody for RIP was purchased from Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were fractionated on 8% (w/v) sodium dodecyl sulfate polyacrylamide gels and transferred to Immobilon®-P PVDF Membrane (Merck, IPVH00010). Membranes were incubated in 5% (w/v ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The tissue was processed as previously reported [26] and proteins were loaded onto 10% polyacrylamide gels and transferred to PVDF sheets (Immobilion-P, MERCK, Burlington, USA). Membranes were blocked with BSA 5% and immunoblotted using primary antibodies for β-Tubulin ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein was concentrated overnight to a volume of 2 mL in a Vivaspin 20 Ultrafiltration Unit (5 kDa MWCO)(Merck, Darmstadt, Germany) and then loaded onto a HiLoadTM 26/60 Superdex TM 75 prep grade (GE Healthcare ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Biophysics 2020Quote: ... The fractions containing protein were combined and a buffer exchange and the protein were concentrated with a 30 K Amicon® Ultra Centrifugal Filter spin column (Merck KGaA) to the storage buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Physiology 2021Quote: ... For colocalization 1:100 anti-NBCe1 was incubated with 1:1,000 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07-623) or 1:200 anti-alpha tubulin (Santa Cruz cat#sc-5286 ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were then transferred by wet tank-blotting (100 V for 60 min) onto a polyvinylidene difluoride (PVDF) membrane (Merck, Darmstadt, Germany). The membrane was blocked with Roti®-Block (2 h at room temperature ...
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bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... at pH 2.0 with final protein concentration of 1.75 mg/mL (Sphβ preparation) or in 40% ethanol (v/v from ethanol absolute, Merck KGaA, Darmstadt, Germany), 0.25 M NaCl at pH 1.8 with final protein concentration of 5 mg/mL (Sphα preparation) ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein from tissue extracts or plasma were prepared in Laemmli or RIPA buffers (both from Merck Life Science UK Ltd, UK), separated by polyacrylamide gel electrophoresis and then transferred to either nitrocellulose or a polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2024Quote: ... For colocalization 1:100 anti-CHRM3 was incubated with 1:500 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07– 623), 1:200 anti-alpha tubulin (Santa Cruz ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein transfer was then visualized using 0.1 % Ponceau S solution (w/v) in 5% acetic acid (Merck Life Sciences, Cat #: P7170-1L) and imaged ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of protein were separated by 10% SDS-PAGE gels and were blotted onto a high-resolution nitrocellulose (NC) membranes (MERCK, Darmstadt, Germany). Membranes were blocked with 5% Albumin Bovine Fraction V (SERVA ...
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bioRxiv - Cancer Biology 2023Quote: ... The separated proteins were transferred from the gel to the polyvinyl difluoride (PVDF) membrane (Immobilon-P, 0.45 µm, Merck Millipore Ltd, Ireland) at 200 mA for 1.5 hours ...
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bioRxiv - Immunology 2023Quote: ... Equal amounts of protein were loaded and separated by electrophoresis on 12% SDS-PAGE gels and subsequently transferred to polyvinylidene fluoride (PVDF; Merck-Millipore, Germany). After blocking with 10% skim milk for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... diluting 3 μl of protein sample (20 nM) in 15 μl PBS buffer loaded on a gasket (Grace Bio-Labs reusable CultureWell gaskets (GBL103250, Merck)) mounted on a glass microscope coverslip (0107222 ...
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bioRxiv - Biochemistry 2023Quote: ... Fusion proteins were expressed in Escherichia coli and subsequently purified using glutathione high-capacity magnetic agarose beads (Merck Millipore GmbH, Darmstadt, Germany) following the manufacturer’s guidelines.
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bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
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bioRxiv - Biochemistry 2024Quote: Cells were harvested by centrifugation at 2000 x g for 15 minutes at RT and resuspended in Lysis Buffer (1X BugBuster® Protein Extraction Reagent (Merck-Millipore), 20 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... The Clone TS2/16 antibody was left incubating overnight and after that the immuno-complex was captured by adding 50μl of protein A/G agarose (Merck Sigma, Burlington, MA) beads for 2 hours at 4°C (with rolling agitation) ...
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bioRxiv - Cell Biology 2022Quote: ... Aliquots were retained for direct Western blot analysis (Input) and otherwise subjected to immunoprecipitation (IP) with protein A-coupled Sepharose beads (Merck, 17-5280-02) pre-incubated with 2 μg anti-RbBP5 antibody or IgG at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Measurements were run in triplicates diluting the protein sample at 50 nM concentration in NMR buffer loaded on a gasket (Grace Bio-Labs reusable CultureWellTM gaskets, Merck (#GBL103250)) mounted on a glass microscope coverslip (Marienfeld (#0107222)) ...
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bioRxiv - Cell Biology 2021Quote: ... The fractions containing the target proteins were pooled and concentrated to less than 0.5 ml using an Amicon Ultra concentrator (Merck Millipore; 10 kDa MWCO). The concentrate was applied to a Superdex 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Neuroscience 2022Quote: Equal amounts of total protein (50 μg) from the Hu14 depleted plasma were filtered using Amicon ultra 3kDa centrifugal filter units (MERCK, New Jersey, USA), dried in speed vac (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of total protein (50 µg) from the Hu14 depleted plasma were filtered using Amicon ultra 3kDa centrifugal filter units (MERCK, New Jersey, USA), dried in speed vac (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... Protein signal was developed using enhanced chemiluminescence according to the manufacturer’s instruction (Immobilon® Forte Western HRP substrate, Merck Millipore Ltd., VIC, Australia) and visualised using the ChemiDocTM XRS system (Bio-Rad ...
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bioRxiv - Biophysics 2019Quote: ... reduced and alkylated protein extracts were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, # MRCPRT010 Ultracel YM-10). Digested samples were desalted using ZipTips according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... cortical sections were incubated overnight at 4 °C with anti-glial fibrillary acidic protein (GFAP, 1:1000, chicken IgG, Merck Millipore, Burlington, EUA), anti-Iba1 (1:200 ...
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bioRxiv - Biochemistry 2019Quote: ... Eluted protein was concentrated using Amicon Ultra-15 centrifugal filter unit with nominal molecular weight limit of 10 kDa (Merck Millipore, Burlington, MA), loaded on a HiLoad 26/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Cancer Biology 2021Quote: ... PDGCLs were lentivirally transduced with the MISSION® shRNA vector pLKO.1-puro-CMV-Turbo green fluorescent protein (TurboGFP)_shnon-target (#SHC016, Sigma, part of Merck, Darmstadt, Germany) for cytosolic TurboGFP expression ...
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bioRxiv - Biophysics 2022Quote: ... equal amounts of GFP and SNAP-tagged recombinant WT-FUS protein (yielding a total protein concentration of 6 μM) were gently mixed into an aqueous solution of 20 % PEG-35 (Merck KGaA, Darmstadt, Germany) and 500 mM KCl (Merck KGaA ...
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Immunology 2023Quote: ... The eluted proteins were buffer exchanged into PBS and then concentrated using Amicon® 30 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9030).
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were concentrated using an AmiconTm Ultra (molecular weight cut-off 30 kDa and 50 kDa) centrifugal filter device (Merck Millipore, Darmstadt, Germany). Fractions from all purification steps were collected and analyzed by SDS- PAGE.
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bioRxiv - Microbiology 2024Quote: ... The purity was controlled by electrophoresing 3 µg of protein with SDS-sample buffer on a polyacrylamide gel (mPAGE™ 4-20% Bis-Tris Precast Gel, Merck, Darmstadt, Germany), followed by Bio-Safe Coomassie staining (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2019Quote: ... Constructs of Kmpv1 and KmpvSP1 were transiently expressed as fusion proteins with GFP on the C-terminus using the liposomal transfection reagent GeneJuice® (MERCK KGaA, Darmstadt, Germany). Measurements were performed at room temperature in a bath solution containing ...
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bioRxiv - Bioengineering 2019Quote: ... 40 µg of total protein were run on a 12% SDS-PAGE and transferred onto a 0.45 µm Immobilon®-P Transfer Membrane (Merck Millipore, Cat. NO#IPVH00010). The membrane was hybridized with a custom antibody at a 1 µg/mL dilution (GenScript ...
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bioRxiv - Microbiology 2022Quote: ... and proteins in the lysate were separated in a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...