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Citations for Merck :
1351 - 1400 of 2136 citations for 6 Chloro N ethyl 2 methylthio 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... embryos were initially incubated with 100 µM EdU (5-ethynyl-2’-deoxyuridine) (Merck, T511285) for 1 h before washing and fixation ...
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bioRxiv - Cell Biology 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Cell Biology 2022Quote: ... Mission siRNA for human Pacsin 2 siRNA (SASI_Hs01_0021-5538, SASI_Hs01_0021-5539 and SASI_Hs01_0021-5540, Merck) or MISSION siRNA Universal Negative Control #1 (SIC-001 ...
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bioRxiv - Cancer Biology 2022Quote: ... special AT-rich sequence binding protein 2 (SATB2; clone EP281, Merck, USA; 1:200), and programmed cell death ligand 1 (PD-L1 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were further concentrated to 2-10 mg/ml using Amicon concentration devices (Merck, 3,000 Da ...
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bioRxiv - Microbiology 2022Quote: ... 0.5% NaCl (Mercury Cat#1064041) and 0.5% yeast extract (Merck Cas#8013-01-2)) supplemented with 250 mg/ml glucose (Mercury Cas#50-99-7) ...
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bioRxiv - Neuroscience 2022Quote: Drugs were used at the following concentrations: 2 µM PMA (Merck Millipore Cat#524400), 1 µM Bradykinin (Sigma-Aldrich Cat#05-23-0500) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg purified recombinant LARK protein or 1 μg BG4 (MABE917, Merck, Darmstadt, Germany) was added ...
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bioRxiv - Neuroscience 2022Quote: ... 1% streptomycin and 2 mM of Glutamax and 0.1 μg·ml−1 GDNF (#SRP3200, Merck) (Vyas et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 2 layers of SCX strong cation exchange Empore™ SPE Disks (Merck). Samples were then lyophilized.
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bioRxiv - Neuroscience 2023Quote: ... The SDC was precipitated from the combined samples using 2% formic acid (FA, Merck).
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bioRxiv - Biochemistry 2023Quote: ... 100mM NaCl using 3k cut-off Amicon® Ultra 2 mL Centrifugal Filters (Merck). Equal amounts of protein from both dialyzed and undialyzed samples were treated with caspase-3 as described above ...
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bioRxiv - Plant Biology 2023Quote: ... then 2% w/v Aspegillus niger pectinase (Merck Life Science UK Ltd., Gillingham, UK) 45°C for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay was used to analyze the cell viability of SH-SY5Y cells ...
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bioRxiv - Biophysics 2023Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosetta 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 µg/ml kanamycin and 34 µg/ml chloramphenicol ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted samples were concentrated using an Amicon Ultra-2 centrifuge filter unit (Merck) and stored on ice.
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bioRxiv - Molecular Biology 2023Quote: ... CaCo-2 cell lines were cultivated in Minimum Essential Medium Eagle (MEM) (Merck, M4655) complemented with 20% FBS Tetracycline free ...
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bioRxiv - Developmental Biology 2023Quote: ... except for a different set of SYBR green primers (Sigma Merck, Supplementary Table 2). The obtained Ct values were analyzed using the ddCt method for relative quantification of mRNA expression derived from three independent experiments ...
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bioRxiv - Neuroscience 2023Quote: ... concentrated using 100 kDa-MWCO Centricon plus-20 and Centricon plus-2 (Merck-Millipore), aliquoted and stored at -80°C ...
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bioRxiv - Cell Biology 2023Quote: ... and concentrated to 65 µL using Amicon Ultra-2 10K filters (Merck Life Science).
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bioRxiv - Biophysics 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Neuroscience 2023Quote: ... and NG2 (neuron-glial antigen 2, AB5320, Merck Millipore, Burlington, MA, USA, 1:400) were used.
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bioRxiv - Bioengineering 2023Quote: ... 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Merck (Germany). The U-87 cell line was a kind gift from Esendagli Group at Hacettepe University (Turkey) ...
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bioRxiv - Microbiology 2024Quote: ... 2–3 mL of stock solution was applied to preparative TLC plates (glass, Merck, 20x20 cm ...
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bioRxiv - Cancer Biology 2024Quote: The 3-(4,5-dimethylthiazo1-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Merck Sigma, Burlington, MA) reduction assay was used to quantify cell proliferation ...
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bioRxiv - Biochemistry 2024Quote: Total ROS levels quantification was carried out using 2′,7′-dichlorofluorescein diacetate (H2DCFDA; Merck) assay ...
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bioRxiv - Developmental Biology 2021Quote: ... after which they were washed three times in PBST and blocked overnight at 4°C in 10% serum (Merck) in PBS/0.3% TritonX100 ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated for 16h at 4°C with 2µL α-H3K27me3 antibody (#07-449; Merck Millipore, Burlington, MA, USA) or α-H3K4-me3 antibody (Rabbit monoclonal antibody (#04–745 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4 °C with a monoclonal anti-RBPMS antibody (1:500, Rabbit, ABN1362, Merck Millipore) for the retina ...
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bioRxiv - Microbiology 2020Quote: ... RNA pellet thus obtained by centrifugation at 16,000 × g for 20 min (4°C) was washed using 70% ethanol (Merck) and resuspended in nuclease-free water after air drying ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4 °C with 100 µL of lysate diluted at 1:40 in carbonate buffer (Merck). After a blocking step with PBS with 4 % milk for 1 h ...
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bioRxiv - Physiology 2020Quote: ... Proteins in the conditioned medium were concentrated up to 1.2ug/ul of total protein using cellulose membrane Ultra-4 Centrifugal Filter Unit of 3KDa cut-off (Merck) as per the manufacturer instructions.
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with a total of 300 ng DNA (per well of a 4-well 3.5 cm dish) using 0.75 µl Genejuice (Merck Millipore) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with Amicon Ultra-4 centrifugal filters (10 kDa cut-off, Merck). The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2019Quote: ... Approximately 4×105 of the cultured cells were then transferred to each well of 96-well filter (Merck Milipore) pre-coated with 2 µg/ml TT in 1x PBS or only 1xPBS ...
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bioRxiv - Biophysics 2020Quote: ... fluorescently-labeled and biotinylated H57-scFV were again concentrated with 10kDa Amicon®Ultra-4 centrifugal filters (UFC801024, MERCK) and stored in PBS supplemented with 50% glycerol at −20°C ...
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bioRxiv - Biophysics 2021Quote: ... cells were washed 2x 4 min in PBS and subsequently overlaid with 0.1 mg/ml collagen IV (C7521, Merck) and placed at 4°C overnight (ON) ...
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bioRxiv - Biochemistry 2021Quote: ... and soluble protein was clarified by centrifugation (50,000×g, 1 h, 4°C) and subsequently passed through a 0.22-μm filter (Merck Millipore).
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bioRxiv - Cancer Biology 2021Quote: Cells grown on coverslips were washed with PBS and incubated 15 min in 4% formaldehyde (formalin solution buffered at pH 6.8, Merck). Cells were washed once with 50 mM ammonium chloride (Carl Roth ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Cell Biology 2021Quote: ... and then equal volumes were concentrated 50-fold using Amicon Ultra-4 centrifugal filter units (Merck Millipore, Watford, U.K.). For analysis of cell-associated proteins ...
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bioRxiv - Microbiology 2021Quote: Cells grown on glass coverslips were fixed in 4% PFA for 30 min at RT and permeabilized with 0.2% Triton X-100 (Sigma/Merck) in PBS for 10 min at RT ...
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bioRxiv - Plant Biology 2021Quote: ... previously equilibrated with 50 mM HEPES buffer (pH 7.0) and concentrated with an Amicon Ultra-4 10K centrifugal filter (Merck).
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bioRxiv - Microbiology 2019Quote: ... The mixture was then centrifuged at 15,000 g at 4 °C and the supernatant was filtered with a 0.22 μm filter (Merck Millipore). A Ni-NTA column was used to purify the recombinant GB1-VpmR-S1 on an AKTA system (GE) ...
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bioRxiv - Bioengineering 2022Quote: ... samples were fixed in a solution of 4 % (v/v) paraformaldehyde supplemented with 1 % (v/v) glutaraldehyde (Merck, USA) overnight at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 108 - 109 plaque-forming units (PFU) mL-1 in tryptone soya broth (TSB, Oxoid) or in quarter-strength (1/4) Ringer’s buffer (Merck) unless stated otherwise ...
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bioRxiv - Physiology 2022Quote: ... containing 150 μL 4°C DMEM (Dulbecco’s modified Eagle’s medium with high glucose and 25mM HEPES from Sigma Aldrich (Merck), UK ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were centrifuged (16000 g, 20 min, 4°C) and filtered using Ultrafree Centrifugal Filter Units (Merck Millipore, USA).
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Synthetic Biology 2021Quote: ... VLP fractions were pooled and concentrated using 100 kDa MW cut-off centrifugal filters (Amicon Ultra 4 ml, Merck). Protein concentrations were measured using the linearised Bradford method54 with Pierce Coomassie Protein Assay Kit (Thermo Scientific).