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Citations for Merck :
1351 - 1400 of 4853 citations for 6 Amino 3 morpholin 4 ylmethyl indazole 1 carboxylic acid tert butyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Viral particles were harvested from the culture medium 6 days after transfection and were concentrated by precipitation with 8% polyethylene glycol 8000 (Merck) and 500 mM sodium chloride ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then plated at a density of 500 cells/cm2 into 6-well plates coated with 1.2% poly(2-hydroxyethylmethacrylate) (Merck, Germany) in 95% ethanol (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatant was then passed through a 3 kDa cutoff centrifugal filter (cellulose Amicon®, Merck) (1 h at 14000 g at 4 ºC ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 mM MgCl2) using Amicon Ultra-0.5 mL Centrifugal Filters (100 K MWCO, Merck Millipore). For each MST assay ...
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bioRxiv - Synthetic Biology 2020Quote: ... The samples were subjected to ultrafiltration using 3-kDa filters (Amicon Ultra 3K device, Merck). After lyophilization ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted fraction was concentrated using a 3 kDa MWCO centrifugal filter (Merck, Darmstadt, Germany). Samples of the column flow-through ...
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bioRxiv - Biophysics 2021Quote: ... to remove DTT and concentrated with a 3 kDa Amicon spin column (UFC500396, Merck Milipore). Antibodies (>1.5 mg/mL ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... Amicon Ultra 0.5 mL centrifugal filter units (molecular weight cut-off 3 kDa; Merck Millipore) were used at 14,000 x g and 4 °C to exchange purified protein samples to 150 mM ammonium acetate (99.99 % ...
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bioRxiv - Neuroscience 2021Quote: ... These flat-mounted strips were fixed in 3% paraformaldehyde (Sigma-Aldrich, Merck, St Louis, USA) with 1.5% glutaraldehyde (Ted Pella ...
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bioRxiv - Neuroscience 2021Quote: ... we embedded the brains in 3-5 % oxidized agarose (Type-I agarose, Merck KGaA, Germany) and covalently cross-linked the brain to the agarose by incubating overnight at 4 °C in 0.5 – 1 % sodium borohydride (NaBH4 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The gelatinous egg sack was removed using 3% L-Cysteine (Merck Millipore, Burlington, MA, USA) and followed by microinjection of viral mimics or shRNAs.
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bioRxiv - Molecular Biology 2021Quote: Cationic liposomes were prepared with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP; Merck Millipore, Darmstadt, Germany), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE ...
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bioRxiv - Microbiology 2021Quote: ... and N-(3-oxododecanoyl)-L-homoserine lactone were purchased from Merck (Sigma-Aldrich, Darmstadt, Germany). Aqueous stock solutions were prepared ...
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bioRxiv - Microbiology 2019Quote: ... India). Sodium alginate (CAS No. 9005-38-3) of medium viscosity was purchased from Merck Pvt ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were transfected with 1.2 µg DNA (total) per 3 µL GeneJuice (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were washed 3 x for 10 min with TBS-T (200 mM Tris (Merck), 1.36 mM NaCl (Merck) ...
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bioRxiv - Plant Biology 2021Quote: ... Pure standards of (Z)-3-hexenyl acetate (Z3HAC, 98 %, CAS number 3681-71-8, Merck) was used in different concentrations ...
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bioRxiv - Biophysics 2021Quote: ... to remove DTT and concentrated with a 3 kDa Amicon spin column (UFC500396, Merck Milipore).
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bioRxiv - Immunology 2020Quote: ... After dialysis external solutions were mixed and were concentrated by Centricon YM-3 (from Merck-Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... 2 M NaCl using a 3 kDa MWCO Amicon Ultra Centrifugal Filter Unit (Merck Millipore) and mixed with tailless Xl histone octamer in the same buffer at a molar ratio 2:1 APLFAD-Δ:histone octamer on ice ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were concentrated using 3 kDa MWCO Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore). Protein concentrations were determined using the Proteins and Labels function on a Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometer and subsequently confirmed on SDS-PAGE gel upon Coomassie staining and colorimetric imaging on an Amersham ImageQuant 800 CCD Imager.
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bioRxiv - Neuroscience 2022Quote: ... we embedded the brains in 3-5 % oxidized agarose (Type-I agarose, Merck KGaA, Germany) and covalently cross-linked the brain to the agarose by incubating overnight at 4 °C in 0.5 – 1 % sodium borohydride (NaBH4 ...
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bioRxiv - Neuroscience 2022Quote: ... The additive was prepared by dissolving D-Glucose-13C6 (389374, CAS 110187-42-3, Merck) as internal standard (IS ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were rinsed with PBS and washed 3 × 15min with 2mM MgCl2 (Merck, Darmstadt, Germany), 0.02% NP-40 (Calbiochem ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, 20 μl, 5 mg/ml, Merck) was added to each well ...
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bioRxiv - Bioengineering 2024Quote: ... the glass surface of the Ibidi chip was methacrylated using 3-(Trimethoxysilyl)propyl methacrylate (Merck Life Science NV ...
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bioRxiv - Molecular Biology 2023Quote: ... the sample buffer was exchanged to water in 3 kDa cutoff Amicon centrifugal filters (Merck). The samples were then concentrated to a final volume of 30 μl and subjected to mass analysis ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the supernatant was transferred to Amicon® Ultra-0.5 3 kDa filter columns (Merck Millipore) and centrifuged at 14,000 × rpm for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated for 3 hours at 37°C with PMA (50ng/ml; Merck Darmstadt) and Ionomycin (1 mM ...
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bioRxiv - Cell Biology 2023Quote: ... and precipitated by supplementing 0.1x volume 3 M sodium acetate (Sigma-Aldrich, Merck, pH 5.3) and a 2x volume −20°C ethanol (VWR Chemicals) ...
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bioRxiv - Physiology 2023Quote: ... Blots were washed 3 x for 7 min with TBS-T (200 mM Tris (Merck), 1.36 mM NaCl (Merck) ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed with 3% PFA (paraformaldehyde, cat#158127, Merck Millipore, Burlington, MA, USA) for 10 min and subjected to immunofluorescence ...
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bioRxiv - Developmental Biology 2023Quote: ... and blocked for 2 hours in PBS-T with 3% bovine serum albumin (BSA, Merck) and 5% normal sheep serum (Merck) ...
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bioRxiv - Neuroscience 2023Quote: ... Drugs were administered intraperitoneally at their corresponding dosages: Yohimbine-Hydrochloride (3 mg/kg, Merck, Germany), Propranolol-Hydrochloride (10 mg/kg ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated for 3 min with Hoechst 33342 dye (Merck Millipore, Billerica, MA, USA) (2µg/ml ...
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bioRxiv - Biophysics 2024Quote: ... The fractions containing GRB2 SH2 domain were concentrated by Amicon Ultra-15 3 kDa (Merck) and then incubated with uTEV3 protease to remove the GST tag in buffer D for approximately 16 h at 25 °C ...
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bioRxiv - Biophysics 2022Quote: ... three washes in PBS+10%HS each lasting 5min and staining of nucleic acids with Hoechst 33342 (5μg/mL-Merck Bio-sciences) for 5min ...
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bioRxiv - Cell Biology 2022Quote: ... the zona pellucida was removed from metaphase I oocytes or metaphase II-arrested eggs by washing cells through Tyrode’s acid solution (Merck, T1788-100ML) droplets covered with mineral oil (52) ...
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bioRxiv - Molecular Biology 2019Quote: To evaluate microscopic features of the captured cells such as morphological types and possible alterations we then used another collection membrane directly stained with hematoxylin and eosin after fixation for 10 min in a fixation solution (100 mL of 70% ethanol, 5 mL of glacial acetic acid and 5 mL of 37% formaldehyde solution – all solutions are from Merck Millipore). Samples were hydrated with distilled water for 5 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... tumefaciens were resuspended in 50 mM MES (2-Morpholinoethanesulfonic acid hydrate) (Duchefa, Haarlem, The Netherlands) - KOH buffer (pH 5.6) containing 2mM NaH2PO4 (Merck, Darmstadt, Germany), 100 µM acetosyringone (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then resuspended in 10 μl of 0.1% trifluoroacetic acid and tryptic peptides were desalted using a Zip-Tip® column (Merck Millipore) pipette tip according to the manufacturer’s protocol and dried in a vacuum concentrator before analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Cancer Biology 2024Quote: ... was followed by 5 minutes of rinsing with water and 20-30 seconds of Eosin solution (Merck, 115935, 100ml supplemented with one drop of acetic acid, Merck, 1.00063). For PAS staining ...
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bioRxiv - Systems Biology 2024Quote: ... The solvent system comprised solvent A consisting of 0.1% formic acid in Milli-Q water (both HPLC grade, Merck, Darmstadt, Germany) and solvent B being methanol (UHPLC grade ...
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bioRxiv - Cell Biology 2023Quote: ... produced by the mix of 50 ml of sodium hypochlorite 10% (Roth, ref. 9062.3) and 2 ml of 37% hydrochloric acid (Merck, ref. 1.00317.1000). Seeds were then aerated for 10 min in a sterile bench to remove the left-over chlorine gas ...
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bioRxiv - Microbiology 2023Quote: ... Samples were passed through a 0.2 µm nylon membrane prior to analysis with liquid chromatography as described previously 50. Separation for organic acid quantification (i.e. SCFAs and intermediate metabolites) was conducted on a LaChrom HPLC-System (Merck-Hitachi, Japan) using a SecurityGuard Cartridges Carbo-H (4 × 3.0 mm ...
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bioRxiv - Cell Biology 2024Quote: Treatment of flies with antioxidant Vitamin C was performed by feeding male flies with food containing 100mM Vitamin C (L-ascorbic acid; Merck – A4403) for the two days before dissection.
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2022Quote: ... Heads including both eyes were fixed in 4% w/v paraformaldehyde (PFA, Merck) in 0.1M phosphate buffer overnight at 4°C and the remaining tissue were kept for genotyping by conventional PCR ...
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bioRxiv - Microbiology 2021Quote: ... concentrated using an Amicon Ultra-4 10 kDa cut-off spin column (Merck), and stored at -80°C in a storage buffer [100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Grids were then stained with 2% (w/v) uranyl acetate pH 4 (Merck) for 1 min ...