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Citations for Merck :
1301 - 1330 of 1330 citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The PDMS particles were generated by vortex-mixing a solution of 1 g PDMS (10:1 w/w, base/curing agent) dispersed in 10ml of 2%w/v poly(ethylene glycol) monooleate (Merck Chemicals GmbH, Germany) aqueous solution ...
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bioRxiv - Microbiology 2022Quote: ... were sequentially filtered by vacuum through hydrophilic polycarbonate filters with a pore size of 20 µm (47 mm; Sterlitech, Kent, WA, US) and then 2 µm (Isopore, 47 mm; Merck Millipore, Cork, Ireland). Filters were immediately plunged into liquid nitrogen and stored at −80°C until further processing ...
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bioRxiv - Genetics 2021Quote: ... Cells were differentiated by LIF withdrawal in DMEM supplemented with 10% FBS and 0.1 mM ß-Mercaptoethanol at a density of 2×104 cells/cm2 on Fibronectin-coated dishes (Merck, 10 µg/ml).
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bioRxiv - Developmental Biology 2021Quote: We mated Exosc10+/+ or Exosc10+/− females with stud Exosc10+/− male mice and recovered two-cell stage (gestational day 2; GD2) embryos by flushing approximately 0.5 ml of EmbryoMax Advanced KSOM medium (Merck-Sigma, MR-101-D) into the oviduct ...
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bioRxiv - Cell Biology 2021Quote: ... BHK-2P maintained either in Glasgow’s Minimal Essential Medium (GMEM) with 10% FBS (“line #2”) or adapted to the ACFM Cellvento™ BHK200 (Merck KGaA, Darmstadt, Germany) in two different processes (lines “#4” and “#5”) ...
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bioRxiv - Microbiology 2022Quote: ... medium was added to the apical side 30min prior to measurement with a chopstick electrode connected to a Volt-Ohm-meter (Millicell® ERS-2, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were pooled and concentrated to a final concentration of ∼2 mg/ml using an Amicon Ultra-4 cellulose centrifugal filter unit (Merck Millipore, Cat# UFC810024).
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bioRxiv - Microbiology 2023Quote: ... 2× overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS/DMEM media and 0.6% (v/v) methylcellulose (Merck Life science, Cat: M0512) to achieve a semi-solid overlay ...
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bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: Mixing of lysozyme crystals (7×2 mm; grown by batch crystallisation) with 25 mM sulfanilic acid azochromotrop (SAA, Merck, lmax 505–510 nm), a highly absorbing red dye ...
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bioRxiv - Neuroscience 2022Quote: ... until reaching the desired staining contrast, and cleared in two xylene baths (2 min, 20 min) before being coverslipped with an entellan-xylene solution (Merck KGaA, Darmstadr, Germany).
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were pooled and concentrated to 500 μl using Amicon Ultra-15 10K MWCO centrifugal filters (Merck Millipore UFC901008; final LDAO = 2%) and further purified by size-exclusion chromatography as described for human VDAC constructs but with 20 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged twice a week using 2 mM ethylenediaminetetraacetic acid in phosphate buffer saline (EDTA/PBS; Thermo Fisher Scientific, Waltham, USA; Merck, Darmstadt, Germany) adding 10 µM Y27632 (Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... injections 3x a week (consecutive days) during 2 weeks of 25 mg/kg 3PO or 75 mg/kg 3PO or dimethyl sulfoxide (DMSO) (Sigma-Aldrich, Merck, Overijse, Belgium) as control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
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bioRxiv - Neuroscience 2023Quote: ... We replenished the medium the next day and started selection against replicating non-neuronal cells on day two using neuronal medium containing 2 μM Cytosine β-D- arabinofuranoside (AraC) (Merck Life Sciences). We gradually diluted AraC out by replacing half of the medium with fresh neuronal medium every other day ...
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bioRxiv - Bioengineering 2023Quote: ... Fractions 7 to 10 which were subsequently identified as the particle fractions (PF) were pooled to a volume of 2 ml and concentrated using an Amicon Ultra 100 kDa centrifugal filter (Merck Millipore, MA, USA), made from regenerated cellulose ...
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bioRxiv - Bioengineering 2024Quote: ... was assessed every day for 7 to 10 days using a Millicell ERS-2 voltammeter EVOM2 and an STX02 chopstick electrode (Merck Millipore, Bayswater Australia). The TEER value was calculated by subtracting the resistance of a blank insert from an insert containing a monolayer and then multiplying it by the surface area of the insert.
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bioRxiv - Cancer Biology 2024Quote: ... The gelatin mixture was prepared by dissolving aliquots of non-fluorescent gelatin (Sigma-Aldrich, Merck, G1890, dissolved in DPBS to 2 mg/mL) and FITC-conjugated gelatin (AnaSpec ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Microbiology 2020Quote: The crude ethylacetate extract of ALR-2 was purified via column chromatography using a LiChroprep RP-18 (15-25 μm) silica gel column (Merck, EMD Millipore Corporation, USA) and chloroform:methanol (ratio 4:1 ...
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bioRxiv - Biochemistry 2021Quote: ... modifying on previous reported conditions for the assay of MjRibK.[2] The reaction mixture was incubated at 37°C overnight and visualized using fluoresce-based thin layer chromatography (TLC) (Merck Silica gel 60 F254) using the long wavelength (365 nm ...
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bioRxiv - Microbiology 2022Quote: ... we used the same approaches as described above and spotted the 5 μl of bacteria on LB agar plates containing 2% of Skim Milk Powder for microbiology (Sigma-Aldrich/Merck KGaA, Darmstadt, Germany). Plates were incubated at 25°C and monitored for hemolytic and protease activities after 1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions containing the desired protein were pooled and concentrated to 2 ml in Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... 48 and 72 hours) of exposure to simulated solar radiation was performed by using 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) (Sigma Aldrich-Merck KGaA, Darmstadtm Germany) solubilized in ethanol ...
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bioRxiv - Developmental Biology 2024Quote: ... and cultured as described in 39 using the following procedure: Cord blood samples were diluted 1:2 in PBS and layered on Ficoll® Paque Plus (Sigma-Aldrich by Merck, Darmstadt, Germany) to isolate mononuclear cells ...
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bioRxiv - Genomics 2024Quote: ... The samples were digested in a closed-vessel microwave system (MarsExpress; CEM Corp; Matthews, NC, USA) in 2 ml of 30% (v/v) premium-grade H2O2 (Merck, EMSURE®, Darmstadt, Germany) and 5 ml of 65% (v/v ...
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bioRxiv - Plant Biology 2024Quote: ... samples were acidified to pH 2 with formic acid and desalted with pipette tips packed with six layers of C18 Empore™ SPE Disks (Merck, cat. no.66883-U) and dried in a Speed Vac at 45 °C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: Cell viability and cytotoxicity of antibiotics and peptides were determined against human corneal epithelial cells (HCE-2, CRL-11135, ATCC, Manassas, Virginia, USA) using cell-counting-kit-8 (CCK-8) assay (Sigma Aldrich, Merck Life Science UK Limited, Dorset, UK) and lactate dehydrogenase (LDH ...
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bioRxiv - Cancer Biology 2024Quote: ... twice with PBS and then pre-coupled tumbling at 4°C for 2 h with antibodies anti-p53 PAb1620 (Merck-Millipore, 0.2 µg/15 µl beads/IP), anti-p53 PAb240 (Merck-Calbiochem ...