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Citations for Merck :
1301 - 1350 of 2128 citations for Tetratricopeptide repeat protein 14 TTC14 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Solubilized total proteins were separated by SDS-PAGE and electro-blotted onto a PVDF membrane (Merck Millipore; Billerica, MA). The membrane was first blocked with 5% skim milk in PBST (Phosphate Buffered Saline with Tween 20) ...
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bioRxiv - Cancer Biology 2020Quote: ... Thirty micrograms of cellular protein were resolved by 10 % SDS PAGE and were transferred to Immobilin P 0.45 μm PVDF membrane (Merck). Bound IgG was visualised with horseradish peroxidase-conjugated secondary antibodies and the Clarity™ Western ECL Substrate (Cat no ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing the capsid protein were pooled and concentrated using an Amicon Ultra-15 centrifugal filter (Merck Millipore), and the buffer was exchanged to a final storage buffer composed of 50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Microbiology 2022Quote: ... HRP-conjugated anti-goat or anti-mouse IgG (dilution 1:1,000) were added and protein complexes were visualized using o-phenylenediamine (Merck). The absorbance was then measured at 490 nm employing PowerWave HT (Bio-Tek Instruments ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was recovered and the protein concentration was estimated using the Direct Detect® Infrared Spectrophotometer (Merck-Millipore). 400 µg protein were incubated with 40 µL of streptavidin coupled DynabeadsTM M-280 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Molecular Biology 2020Quote: ... Total protein content in each eluate was measured using infra-red spectroscopy (Direct Detect, Merck Millipore, Burlington, MA, USA). The remainder was aliquoted and stored at −80 °C.
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bioRxiv - Biochemistry 2020Quote: ... Samples were subjected to SDS-PAGE and proteins were transferred to Polyvinylidene difluoride (PVDF) membranes (Immobilon®-P, Merck). The ATP2 proteins fused to the BAD tag were detected with the Avidin HRP-conjugate probe (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... the protein extracts (15 μg/sample) were blotted on a primed 0.45 μm polyvinylidene fluoride (PVDF) membrane (Merck-Millipore). Protein spots were stained using Direct Blue (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... and 4.2 μg PARG (in-house) per 10 mg protein and 10 μl of Benzonase (Merck Millipore, Darmstadt, Germany) per 30 ml were then added to each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing the proteins of interest were pooled and concentrated using Amicon Ultra-15 30K spin concentrators (Merck Millipore) before further purification using a HiLoad 16/600 Superdex 200 prep grade size exclusion column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was used to determine the total protein concentration by the Bradford method (Bradford reagent, Merck, Darmstadt, Germany), in a microplate reader at 595 nm ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Genomics 2022Quote: ... Total chromatin was pre-cleared for 1h at 4°C with rotation using protein A sepharose beads (P9424, Merck). 3 µg of anti-histone H3 (Abcam ...
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bioRxiv - Immunology 2020Quote: ... Collected proteins were buffer exchanged into PBS via multiple rounds of centrifugation in 10 kDA ultrafiltration tubes (Merck, USA). The purified protein content was measured by Bradford assay (B6916 ...
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bioRxiv - Immunology 2020Quote: ... 20 μg of brain lysate was examined by SDS-PAGE and proteins were blotted on PVDF membrane (Immobilon, Merck). Membrane was blocked with 2% skim milk and subsequently incubated with anti-DJ-1 antibody overnight ...
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bioRxiv - Immunology 2020Quote: ... and the fractions with the target protein were mixed and concentrated using Amicon Ultra-15 Centrifugal filters (Merck Millipore) with a 3 kDa membrane cutoff (Figure 10).
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bioRxiv - Immunology 2021Quote: ... the protein content of the supernatants was concentrated 10x using centrifugal filters with 10 kDa cutoff (#UFC501096, Amicon, Merck). The concentrated samples were boiled in a reducing sample buffer and separated in 4-12% polyacrylamide gel (#NW04122BOX ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were collected at 20,000 x g for 40 min at 4°C and soluble proteins in the supernatant were sterile filtered 5µm PVDF Membrane (Merck).
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bioRxiv - Microbiology 2021Quote: ... proteins were separated using a 10% SDS-PAGE gel and transferred onto a PVDF Immobilon-P membrane (Merck Millipore). The membrane was blocked with 5% w/v milk in TBST (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... Peak fractions containing SETDB1 Triple Tudor protein were pooled and concentrated to 2 mL in Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... with the resulting protein pellet resuspended in Tris buffer and sterilized using 0.45 μm and 0.2 μm syringe filters (Merck, US).
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bioRxiv - Cancer Biology 2023Quote: Proteins were extracted by RIPA lysis buffer (EcoTech Biotechnology, RIPA-100) containing cOmplete™ protease inhibitor cocktail (Merck, 11697498001), PMSF (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... Expression of mScarletI-PXVam7 from the pCUP1 promoter or of down-regulated proteins from the weak pCUP1(dim) promoter was induced by supplementation of 10μM (unless noted otherwise) copper sulfate (Merck) to the growth medium ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Citrullination of proteins was induced via activation of PADI4 by the addition of calcium ionophore Af23187 (cat. C7522, Merck) to a final concentration of 4 µM ...
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bioRxiv - Cell Biology 2023Quote: ... Separated proteins on the gels were blotted on Amersham™ Hybond® low-fluorescence 0.2 µm PVDF membranes (Merck). The membranes were blocked using fluorescence blocking buffer (Azure Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein concentration of lysates was determined using a bicinchoninic acid kit (Merck Life Science UK Ltd, Gillingham, UK) and samples diluted in Laemmli buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein pellets were resuspended in 1x S-Trap lysis buffer (5% SDS, 50 mM TEAB from Merck, pH 8.5). 50 µg of the sample protein was digested using the S-trap micro spin column digestion protocol from ProtiFi LLC ...
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bioRxiv - Immunology 2024Quote: ... 50 μL/well of plex beads were distributed in a MultiScreenHTS BV Filter Plate (1.2 μm Hydrophilic Low Protein Binding Durapore Membrane, Merck) and washed three times with 120 μL of ABE buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced utilizing a 5 mM solution of tris-2-carboxyethyl-phosphine (TCEP) (Merck KGaA, Darmstadt, Germany) at a temperature of 60 °C for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated using a centrifugal filter unit (Amicon Ultra, 10 or 30 kDa cut-off, Merck), and the solvent was exchanged with 50 mM potassium phosphate buffer (pH 7.0 ...
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bioRxiv - Biochemistry 2023Quote: ... was performed on 4 and 2.5 mg protein lysates for timsTOF optimization and dual-probe analyses respectively in 2 M urea (Merck) in 1× 50 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... The bound protein complexes were washed thrice with NETN buffer and then eluted with 2 mg/mL biotin (Merck) for 90 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was collected as control and the beads were thoroughly washed 3 x with TBS/Tween 0.2% after which bound proteins were eluted by adding hot reducing SDS sample buffer containing 2-mercaptoethanol (Merck) and boiling for 5 min at 99 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... protein concentration was determined by IR spectrometry measuring amide bond absorbance using a Direct Detect IR Spectrometer (Merck KGaA). If protein concentration (of the undiluted lysate ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred from the gel to a PVDF membrane (Immonilon-P transfer membrane, Merck, Darmstadt, Germany) using the wet transfer method ...
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bioRxiv - Microbiology 2022Quote: ... and protein expression was induced O/N with 0.5 mM isopropyl 1-thio ß-D-galactopyranoside (IPTG) (Merck Chemicals). The next day ...
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bioRxiv - Microbiology 2022Quote: ... Different amount of protein samples were electrophoresed on 10% SDS-PAGE and then transferred to PVDF membranes (Millipore, Merck) by electroblotting ...
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bioRxiv - Biophysics 2023Quote: ... Protein extracts (50 μg/lane) were resolved in 15% SDS-PAGE followed by immunoblotting using anti-GFP (AB3080P, Merck), anti-PrPC monoclonal antibody (anti-CD320 clone 6D11 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein complex-containing fractions were collected and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated protein was split into two samples ...
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bioRxiv - Physiology 2023Quote: ... For citrate synthase activity, 15-30 µg protein was added to the reaction buffer (20 mM Tris-HCl, pH 8.0 (Merck), 0.42 mM acetyl-coenzyme A (Sigma) ...
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bioRxiv - Plant Biology 2023Quote: ... Protein content of supernatant samples were normalized and analyzed using SDS-PAGE (mPAGE Bis-Tris 8%, Merck, Darmstadt, Germany) revealing specific protein bands in the supernatant while comparing wildtype and the T2SS mutant (xpsxcs ...
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bioRxiv - Plant Biology 2023Quote: ... Purified proteins were concentrated to 10–30 mg/ml by using a 10-kDa Amicon centrifugal filter device (Merck), flash-frozen in liquid nitrogen ...
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bioRxiv - Cancer Biology 2024Quote: Protein solutions were first diluted with 50 mM ammonium bicarbonate (ABC) and reduced with 5 mM dithiothreitol (DTT, Merck) at 60 °C for 45 min ...
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bioRxiv - Microbiology 2024Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Microbiology 2024Quote: ... The pellet containing the FoIV1-rORF4 inclusion bodies was obtained by centrifugation at 12,000 × g for 20 min at 4°C and then suspended in 0.1× BugBuster Protein Extraction Reagent (Merck KGaA) using a homogenizer ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from cells or tissues by RIPA buffer supplemented with a protease inhibitor cocktail (Merck, #11836170001) and phenylmethanesulfonyl fluoride (Beyotime ...
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bioRxiv - Plant Biology 2024Quote: ... ADPG2 and PGLR proteins were concentrated using an Amicon Ultra Centrifugal filter with a 10 kDa cut-off (Merck Millipore ...