Labshake search
Citations for Merck :
1301 - 1350 of 1351 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... one stationary liquid culture was poured into a 50 mL syringe and plunged through a sterile 5 μm membrane filter (Millex-SV, Merck, Darmstadt, Germany), with slow and consistent pressure ...
-
bioRxiv - Microbiology 2024Quote: ... for 10 minutes in the dark and then filtered through Nuclepore black polycarbonate membrane filters (0.22 µm pore size, Merck Millipore, Darmstadt, Germany) with negative pressure (≤ 20 kPa) ...
-
bioRxiv - Biochemistry 2023Quote: PrPC was filtered through a 100 kD membrane filter in 10 mM Bis Tris pH 6.5 (Amicon ULTRA 0.5 mL 100K 96PK, UFC510096, Merck Life Science UK Ltd) to remove aggregates and then diluted into reaction buffer (50 mM Na-phosphate ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by incubation on ice for 10 min and loaded on SDS-PAGE gel and wet transfer onto PVDF membrane (Merck, Cat. # IPFL00010). Thereafter ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNAs were chemically cross-linked to the membrane for 90 minutes at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Merck, Sigma Aldrich). The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
-
bioRxiv - Cell Biology 2022Quote: ... Separated proteins were then transferred by wet tank-blotting (100 V for 60 min) onto a polyvinylidene difluoride (PVDF) membrane (Merck, Darmstadt, Germany). The membrane was blocked with Roti®-Block (2 h at room temperature ...
-
bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...
-
bioRxiv - Microbiology 2022Quote: ... Dust samples were collected by impaction on preconditioned and pre-weighted 25 mm hydrophilic polycarbonate membrane filters with a pore size of 0.8 µm (Merck KGaA, Darmstadt, Germany) mounted in antistatic polypropylene total dust filter cassettes (Pall Laboratories ...
-
bioRxiv - Biophysics 2024Quote: ... Channel subunits in streptavidin precipitates and total lysates were analysed by Western blotting on 8% sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels and transferred onto a polyvinylidene fluoride membrane (Merck, Milan, Italy).
-
bioRxiv - Neuroscience 2024Quote: ... Hippocampi were dissected and sliced into 350 um thick transverse slices with a use of McIlwain tissue chopper and placed on PTFE membranes (Millicell; Merck, Cat# PICMORG50). Cultures ware kept in the air-medium interphase ...
-
bioRxiv - Cancer Biology 2024Quote: ... 09499-14) were subjected to SDS-PAGE on a 10% polyacrylamide gel and subsequently transferred to Immobilon-P PVDF-membrane (Merck Millipore, IPVH85R). The membrane was blocked with 5% (w/v ...
-
bioRxiv - Microbiology 2024Quote: ... Purification and concentration of □ ERS-1 was carried out with Amicon® Ultra-15 centrifugal filter with Ultracel100 kDa membrane (Merck Millipore) (Sun et al. ...
-
bioRxiv - Immunology 2024Quote: ... And then the proteins were separated on 12.5% SDS-PAGE gels and then transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking with 5% nonfat milk diluted in Tris Buffered Saline with Tween 20 (TBST ...
-
bioRxiv - Immunology 2024Quote: ... and the number of neutrophils that migrated through the membrane was counted on a flow cytometer using Muse Count & Viability Kit (#LUMIMCH100102, Merck, NJ, USA) according to the producer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... The membrane was rinsed with TBS buffer and incubated with the primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) (1:2,500 ...
-
bioRxiv - Biophysics 2024Quote: ... 500 ng of BiP were separated via SDS-PAGE on 10 % polyacrylamide gels (homemade) and transferred on Immobilon®-P PVDF membranes (Merck-Millipore) for 1.5 h at 230 mA using a Biometra Fastblot B44 (Analytik Jena ...
-
bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
-
bioRxiv - Cell Biology 2022Quote: ... the SDS samples were applied onto an Amicon filter unit (MWCO = 10 kDa; Ultracel-10 cellulose membrane, Cat.No; UFC201024, Merck Millipore, Billerica, MA, USA) to trap the proteins ...
-
bioRxiv - Immunology 2022Quote: ... A 15 ul of lysate was resolved on 12% SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Burlington, MA). The membrane was blocked in 5% powder milk overnight in 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... and HA-tagged proteins of samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were transferred to a PVDF membrane (Merck Millipore, Tokyo, Japan). The membrane was incubated with rabbit anti-N antiserum or mouse anti-GFP antibody ...
-
bioRxiv - Plant Biology 2021Quote: ... The dried extracts were dissolved in 350 μl purified water and filtered through MultiScreen PCR-96 Filter Plate membranes (Merck Millipore, Darmstadt, Germany) to remove high-molecular-mass compounds ...
-
bioRxiv - Cell Biology 2020Quote: ... The pooled fractions were transferred to ice-cold PBS pre-rinsed Amicon Ultra-4 centrifugal filter units with Ultracel-30 membrane (Merck Millipore, Massachusetts, USA), then centrifuged with 3,200 x g at 4 °C for 10 min ...
-
bioRxiv - Biochemistry 2020Quote: ... The resulting cell free supernatant (CFS) was concentrated through a 10 kDa polyethersulfone membrane disc using an Amicon® Stirred Cell (Merck Millipore, Germany) to a final volume of 50 mL ...
-
bioRxiv - Cell Biology 2020Quote: ... were separated with a 10% SDS-PAGE resolving gel using the Laemmeli discontinuous buffer system and then transferred to a PVDF transfer membrane (Immobilon-FL, Merck KGaA, Darmstadt, Germany) using a Biorad wet transfer apparatus ...
-
bioRxiv - Microbiology 2020Quote: ... One hundred mL of sewage samples were filtered with a 0.22-μm-pore-size mixed cellulose ester membrane (Merck Millipore, Billerica, MA, USA). DNA was extracted from the filtered membranes and from 150–200mg of the nonhuman fecal samples using the ZR Fecal DNA MiniPrep™ Kit (Zymo Research ...
-
bioRxiv - Neuroscience 2023Quote: ... Four small insertions were made and the retina was flat mounted on a filter paper annulus (1 mm inner radius; 0.8 µm hydrophilic MCE MF-MilliporeTM membrane filter, Merck Millipore Ltd., Tullagreen, Ireland). The retina was then mounted photoreceptor cell side up on a perforated 60-electrode MEA chip (60pMEA200/30iR-Ti ...
-
bioRxiv - Biophysics 2024Quote: ... The phages were then purified with two different protocols: Protocol 1: Phage lysates were filtered through 0.22 µm polysulfone membrane filters (Merck Millipore, Billerica, MA, USA) and were used for subsequent purification and concentration by cross-flow filtration using Sartorius Hollow fiber cartridges ...
-
bioRxiv - Microbiology 2023Quote: ... Cell-free extracts were then resolved by SDS-PAGE using 12.5%-15% polyacrylamide gels and subsequently electro-blotted onto a 0.45 μm polyvinylidene difluoride (PVDF) Immobilon®-P membrane (Merck Millipore, Sigma Aldrich) at 100 V for 90 minutes ...
-
bioRxiv - Plant Biology 2024Quote: ... and then clarified via passing through Millex® -GP Fast Flow & Low Binding Millipore Express® PES Membrane 0.22 µm syringe filter unit (SLGP033RS, Merck Millipore, USA).
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were visualized using Fusion Fx (Vilber Lourmat, Marne-la-Vallée, France) with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Billerica, MA, USA). Protein levels were quantified using ImageJ and normalized to the β-actin levels.
-
bioRxiv - Plant Biology 2024Quote: ... The seedlings were cut perpendicularly in the upper hypocotyl with an injection needle tip (NN-2613S; TERUMO, Tokyo, Japan) on a membrane filter (HAWP04700; Merck Millipore, Darmstadt, Germany). The obtained scion was connected with the partner rootstock through a 0.4-mm silicon microtube (1-8194-04 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... A thin film of excised bronchial tissue from rats was evenly distributed in the space between a pair of semi-penetrating membrane filters (Merck Millipore, 2.0 μm) placed between the donor and acceptor chambers ...
-
bioRxiv - Cell Biology 2024Quote: ... Equal amounts of proteins per sample were subjected to SDS-PAGE and transferred to Immobilon-PSQ PVDF transfer membrane (Merck Millipore, Darmstadt, Germany) with Trans-Blot Turbo Transfer System (Bio-Rad ...
-
bioRxiv - Biochemistry 2022Quote: ... then the acid supernatant was transferred to a 30 kDa centrifuge spin filter (Microcon-30 kDa Centrifugal Filter Unit with Ultracel-30 membrane, Merck, Australia; Product ID: MRCF0R030) prior to further centrifugation at 3,700 rpm for approximately 30 minutes until liquid had passed through the filter membrane ...
-
bioRxiv - Microbiology 2022Quote: ... and proteins in the lysate were separated in a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
-
bioRxiv - Cell Biology 2022Quote: ... Equal amount of lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride membrane (Merck Millipore, Burlington, MA, USA) in 25 mmol/L Tris ...
-
bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... were separated through electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel and then transferred onto an Immobilon-P membrane (Merck Millipore, Burlington, MA, USA). After blocking with 5% bovine serum albumin ...
-
bioRxiv - Microbiology 2023Quote: ... The membrane was washed 3 times in TBS before being probed with protein A-peroxidase (Merck, 1:50000 in 10% Skimmed Milk in TBS). The membrane was incubated with 1x LumiGLO® / 1x Peroxidase (CellSignal ...
-
bioRxiv - Pathology 2022Quote: ... 50 μg of protein was separated via SDS-polyacrylamide gel electrophoresis (SDS–PAGE; Bio–Rad Laboratories, Inc., Hercules, CA, USA) and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Burlington, MA, USA). Then ...
-
bioRxiv - Microbiology 2023Quote: ... approximately 300 µL supernatant was passed through a 0.2 µm nylon membrane filter (AcroPrep™ Filter plate; Pall Corporation, New York, United States; or syringe filters; Merck & Cie KmG, Schaffhausen, Switzerland). Analysis with a sample volume of 40 µL was carried out using a Chromaster HPLC-System (Hitachi ...
-
bioRxiv - Cell Biology 2023Quote: ... Gels were run in 1x running buffer and then the proteins were transferred from the gel in ice cold 1x Buffer onto Immobilon®-P Transfer membranes (Merck Millipore, Cat.No. IPVH00005). Transfer membranes were air dried to dehydrate and stored until use ...
-
bioRxiv - Cell Biology 2024Quote: ... An aliquot of 30 μL from each sample was subjected to electrophoresis on 8% or 15% polyacrylamide gel for ARAP1 and ADAP1 each and transferred into an Immobilon®-P transfer membrane (Merck Millipore Ltd., Germany). Membranes were blocked with 5% skim milk (Nacalai Tesque ...
-
bioRxiv - Neuroscience 2024Quote: ... each sample was separated on 5-20% gradient e-PAGEL mini gels (ATTO Corporation, Tokyo, Japan) and transferred to polyvinylidene fluoride membranes (Merck Millipore, Billerica, MA, USA). The membranes were incubated for 30 min with blocking buffer (Nacalai Tesque) ...
-
bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
-
bioRxiv - Microbiology 2023Quote: Proteins in the lysate were separated on a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins in 65 μl of conditioned media and cell lysates per lane were separated on 6% polyacrylamide gels using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred on a poly-vinylidene difluoride (PVDF) membrane (Immobilon-FL, Merck Millipore Ltd., Burlington, MA) using the MiniProteas Blotting Module (Bio-Rad ...
-
bioRxiv - Biochemistry 2024Quote: ... Pooled fractions were concentrated to 20 mg mL-1 using a 10-kDa cut off filtration membrane (Cat. UFC501096, Merck Millipore, Burlington, MA, USA). Sitting drop crystallization trials were set up by depositing 150 nL of the precipitant solution on top of 150 nL of protein with 70 μL of the reservoir using a Crystal Gryphon LCP robot (Art Robbins Instruments) ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein concentration was measured for each sample on Direct Detect® Assay-free membrane cards using a Direct Detect® Spectrometer (both Merck Millipore, Burlington, MA, USA). RIPA lysate containing 50µg total protein was processed for SDS gel electrophoresis ...