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Citations for Merck :
1301 - 1350 of 5258 citations for 4 Bromo 3' 1 3 dioxolan 2 yl 2 fluorobenzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... UK) previously coated with calf-skin collagen (15 μg/cm2 and fibronectin 3 μg/cm2; both Merck Life Science UK Ltd.). The permeability of hCMEC/D3 cell monolayers to 70 kDa FITC-dextran (2 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... 5-DIV cultured human spinal motor neurons and C5-C7 ventral horns of adult animals 3 days after surgery were estimated using a Rac1/Cdc42 Activation Assay Kit (#17-441, Merck Millipore). Briefly ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
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bioRxiv - Immunology 2020Quote: ... Peptides were separated from the HLA molecule remnants by ultrafiltration employing 3 kDa and 10 kDa Amicon filter units (Merck Millipore) for HLA-I and HLA-II ...
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bioRxiv - Microbiology 2020Quote: ... primers 1022700 5F and R were used to amplify the 572 bp 5’ homology flank and primer pair 1022700 3F and R was used to amplify the 673 bp 3’ homology flank (KOD Hot Start DNA Polymerase, Merck Millipore) which were cloned on either side of the sfGFP expression cassette in pkiwi003 (Ashdown et al. ...
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bioRxiv - Microbiology 2020Quote: ... The media was further concentrated to ~3 ml with a 100 kDa cut-off centrifugal concentrator filter (Amicon Ultra, Merck Millipore) and layered onto a 20-60% w/v sucrose density gradient in PBS buffer ...
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bioRxiv - Biochemistry 2021Quote: ... bacteria were grown to mid-logarithmic phase at 37 °C in 3% (w/v) tryptic soy broth (Merck KGaA, Darmstadt, Germany). The microbial culture was washed twice by centrifugation and re-suspended in fresh 10 mM Tris buffer (pH 7.4 at 37 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Eluted protein samples were combined in one tube and desalinated using an ultrafiltration tube (Amicon Ultra 3 kDa molecular weight cut-off, Merck/Millipore) with Buffer C (50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... In the first tube, 6 ml of amniotic fluid was dissolved in 3 ml of glycerol (≥99 %, G2025, Sigma-Aldrich (Merck), Overijse ...
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bioRxiv - Microbiology 2023Quote: ... Clear lysates were subsequently filtered through 10 kDa followed by 3 kDa Amicon Ultra-0.5 centrifugal filters (Merck KGaA, Darmstadt, Germany) to generate sub-cellular fractions with molecular size of larger than 10 kDa ...
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bioRxiv - Cell Biology 2022Quote: ... they were mechanically disrupted by a 21G needle inside a 3 ml DLD dissociation solution (PluriSTEM® Dispase-II Solution, Liberase, #5401119001 DNase I, #10104159001, all from Merck), and incubated for 20-30 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were subsequently washed 3 times for 10 minutes with PB containing 0.2 % Triton X-100 (Merck, Darmstadt, Germany, 108603; PBT). Next ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were subsequently washed 3 times for 10 minutes with PB containing 0.2 % Triton X-100 (Merck, Darmstadt, Germany, 108603; PBT). Next ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.6) for 30 min at RT before incubation with blocking solution consisting of 3% bovine serum albumin (Sigma, Merck, Germany, #A9418) and 0.3% Triton X-100 in PBS 1X ...
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bioRxiv - Plant Biology 2023Quote: ... the dried pellets were redissolved in 80 % methanol (1µL/mg) and 3 µL were analysed by HPTLC on a Silica Gel 60 plate (Merck, Darmstadt, Germany). The mobile phase used was ethyl acetate ...
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bioRxiv - Molecular Biology 2023Quote: ... and dialyzed against cold sterile PBS using an Amicon Ultra-15 Centrifugal Filter Unit with a 3 kDa MWCO (Merck Millipore). The resulting product was then passed through a 0.22 µM filter.
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bioRxiv - Molecular Biology 2023Quote: ... Eluted fractions were concentrated and dialyzed against PBS using Amicon Ultra-15 Centrifugal Filter Units with a 3 kDa MWCO (Merck Millipore). Endotoxins were removed using High Capacity Endotoxin Removal Spin Columns (Pierce) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... were homogenized (60 Hz, 90 s) and extracted by ethanol three times (95%, 3 × 300 mL, vortex 10 min each, Merck, Germany). The three batches of extracts were pooled together and centrifuged for 20 min at 10000g and 4°C (Bruker ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Evolutionary Biology 2023Quote: Total RNA was extracted from ∼150 injected animals (3 days old planulae) with the aid of Tri- Reagent® (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed 3× five minutes in BSA-T and incubated with an antibody against the FLAG-tag (#F1804 Sigma-Aldrich, now Merck, or #14793 Cell signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral transductions were performed in a 6-well plate format (3 × 105 cells/well) using 10 µg/mL polybrene (Merck Millipore). Stably transduced cells were flow-sorted.
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bioRxiv - Biochemistry 2022Quote: ... 300 mM NaCl) using a 3 kDa (or 10 kDa) molecular weight cut-off (MWCO) Amicon Ultra Centrifugal Filter Unit (Merck Millipore), and used directly or aliquoted ...
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bioRxiv - Synthetic Biology 2022Quote: ... Eluted protein samples were combined in one tube and desalinated using an ultrafiltration tube (Amicon Ultra 3 kDa molecular weight cut-off, Merck/Millipore) with Buffer C (25 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting sample of holo-SmLPMO10A was concentrated to ~ 150 μM using an Amicon ®Ultra centrifugation filter (3 kDa cut-off, Merck) and the sample was split into three equal volumes of ~ 500 μL ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was removed (around 25 ml) and either concentrated 10-fold with Amicon 3 kDa spin columns (Merck, cat. #UFC900308) and brought to a final volume of 2 ml for subsequent Western blot analysis (Fig.1B ...
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bioRxiv - Cell Biology 2024Quote: ... AT2 Organoids were fixed with PFA for 3 minutes and then released from matrigel by incubating the culture with Corning Cell Recovery Solution (Merck, CLS354253) for 2 hours at 4 °C.
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bioRxiv - Bioengineering 2024Quote: ... or 3-5×105 pelleted cells were lysed in TRI Reagent (“LS” for fluid EV samples, conventional for cells, Merck Millipore). Liquid chloroform was added to the lysates at a 1:5 v/v ratio and vigorously mixed for 30 s ...
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bioRxiv - Cell Biology 2024Quote: ... The next morning cells were transfected with 10 µg of the plasmid of interest using GeneJuice transfection reagent (Merck, #70967-3) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... Fractions containing FF A39G were concentrated and exchanged in the NMR buffer (100 mM sodium acetate, 200 mM sodium chloride, pH = 5.7) with Amicon Ultra 15 MWCO = 3 K (Merck, Darmstadt, Germany). The cleaved protein product was 71-residues long with no cloning artifacts at either end (GSQPAKKTYT WNTKEEAKQA FKELLKEKRV PSNASWEQGM KMIINDPRYS ALAKLSEKKQ AFNAYKVQTE K).
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bioRxiv - Cell Biology 2024Quote: ... The cell culture medium was concentrated using Amicon® Ultra-15 3 K centrifugal filters (Merck Millipore Ltd., Burlington, MA, USA) into a final volume of 80 ml ...
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bioRxiv - Cell Biology 2024Quote: ... This was achieved by buffer exchange with PBS buffer using Amicon® Ultra-0.5 3 K centrifugal filters (Merck Millipore Ltd.).
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bioRxiv - Molecular Biology 2021Quote: WT MtbRho and the M495L mutant were overexpressed in Rosetta 2(DE3) cells (Merck-Millipore) harboring the appropriate pET28b derivative and purified following published protocols35 with minor modifications ...
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bioRxiv - Biochemistry 2020Quote: ... and a SeQuant ZIC-HILIC precolumn (5 μm particles, 20 × 2 mm) (Merck, Darmstadt, Germany) using a linear gradient of mobile phase A (5 mM NH4OAc in acetonitrile/H2O (5/95 ...
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bioRxiv - Biochemistry 2021Quote: Ethanol (absolute for analysis) and 2-propanol (for analysis) were obtained from Merck (Darmstadt, Germany). Sodium chloride (≥99.8%) ...
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bioRxiv - Cell Biology 2021Quote: 6 μL of 70 kDa FITC-dextran in EGM-2 (5 mg/mL, Merck, #46945) was added to each vessel ...
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bioRxiv - Cell Biology 2021Quote: ... Intracellularly applied drug: the membrane impermeable G-protein blocker GDP-β-S (2 mM, Merck) (Farkas et al. ...
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bioRxiv - Cell Biology 2020Quote: ... at a final concentration of 10 ng/ml and phosphatase inhibitor cocktail-2 (P5726, Merck) at 1% (v/v) ...
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bioRxiv - Immunology 2022Quote: ... EB retained in the brain tissue was extracted by immersion in 2 ml formamide (Merck) at 37°C in the dark for 48 h ...
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bioRxiv - Biochemistry 2022Quote: ... and the cells grown in 2 L of Overnight Express Instant LB medium (Merck, Germany) containing 100 μg/ml ampicillin at 28 °C for approximately 32 h ...
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bioRxiv - Biophysics 2022Quote: ... bis-acrylamide (2% w/w) and ammonium persulfate (0.05% w/v) (all from Merck, Germany) in 10 mM Tris-buffer (pH 7.48) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Acetylthiocholine iodide (ATCh) and 5,5’-dithio-bis-2-nitrobenzoic acid (DTNB) were obtained from Merck (https://www.sigmaaldrich.com/united-kingdom.html ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μL of extract containing total lipids were separated on TLC (Silica Gel 60, Merck) by using petroleum ether:diethyl ether (90:10 ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos from in vitro culture were fixed in a solution containing 2% paraformaldehyde (PFA, Merck) for 20 min at 37° C [41] and ex vivo flushed embryos were fixed in 4% PFA over night at 4° C ...
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bioRxiv - Microbiology 2023Quote: All compounds (zaprinast, heparin, artemisinin, chloroquine, brefeldin A and Torin 2) were sourced from Merck KGaA ...
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bioRxiv - Biophysics 2023Quote: ... RNA from yeast diluted in PBS (0.7 and 2 mg/mL; Sigma-Aldrich, Merck #10109223001).
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bioRxiv - Microbiology 2023Quote: ... 300 µL of aqueous phase was added to 500 µL of 2-propanol (Merck, Germany) and kept overnight at −20°C ...
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bioRxiv - Bioengineering 2023Quote: ... followed by incubation in 10 mL of 2 U/mL dispase solution (D4693; Merck KGaA) at 4 °C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Maize was cultured in ¼ Hoagland’s medium (Hoagland’s No. 2 Basal Salt Mixture, Sigma-Aldrich/Merck), pH adjusted to 5.6-5.8 with KOH ...
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bioRxiv - Immunology 2022Quote: ... Peritoneal cavity cells were obtained by lavage with 5 mL PBS + 2 mM EDTA (Merck). Following gentle massage ...