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Citations for Merck :
1251 - 1300 of 1348 citations for Recombinant Human CD276 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the protein of interest were pooled and concentrated using an Amicon® Ultra-4 3K centrifugal filter unit (Merck) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE and were blotted onto an Amersham™ Protran® nitrocellulose membrane (Merck KGaA; Darmstadt, Germany) for 1.5 h using const ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were washed three times with TBST for 10 min and proteins were detected via chemiluminescence using Immobilon® Forte Western HRP substrate (Merck). For the multiplex detection of native LpxC and the LPS level 12% stain-free FastCast Acrylamide gels (BioRad ...
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bioRxiv - Physiology 2023Quote: ... For protein extraction the tissue was homogenised in RIPA buffer with a cocktail of phosphatase inhibitors (Sigma-Aldrich, Merck, Gillingham, Dorset), using a sonicator three times for 30s each time ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Immunology 2023Quote: ... were detected using rabbit primary antibodies specific to the MOPV Z protein (Agrobio) and secondary alkaline phosphatase-conjugated antibodies (Sigma-Aldrich Merck). Plates were revealed using an NBT/BCiP solution (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Microbiology 2023Quote: ... Cells were permeabilized with 100% ice-cold methanol for 20 min at -20°C and rehydrated by washing with PBS and then stained for viral hexon protein expression with an anti-hexon primary antibody (mouse; Merck; #MAB8052) and an anti-mouse secondary antibody coupled to AF488 or AF647 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell lysates were separated via SDS-PAGE and transferred to Immobilon-P PVDF membranes (IPVH00010, Merck & Co., Inc., USA). These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2023Quote: ... The protein sample obtained after Ni-NTA was concentrated up to 5 ml using a centrifugal filter unit (Merck & Co., USA) with a cutoff size of 10 kDa ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 50 μg of proteins were separated by 10% or 15% SDS- polyacrylamide gel (SDS-PAGE) and transferred to a nitrocellulose membrane (Merck, HATF00010). To detect the CXCL16 and CXCL8 secreted in the culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were wet-blotted (110 V; 90 min; 4°C) on fluorescence-optimized polyvinylidene difluoride (PVDF) membranes (Immobilon-FL; Merck; IPFL00010) in transfer buffer (25 mM Tris (pH 7.6) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein G Dynabeads (catalogue # 10003D) were washed with 0.02% (v/v) PBS-Tween 20 (Tween-20: catalogue # P2285, Merck; 0.01M PBS) and incubated with 200µL of primary antibody mix (for antibody details see Supplementary Table 5 ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg of protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto Immobilon®-P PVDF membrane (Merck), followed by blocking in milk and further overnight probing at 4 °C with a primary antibody specific for Cre (1:500 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The rest of the supernatant for each sample is used to measure the total protein level of the sample with a Bradford assay (Merck # B6916), following reagent protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... the solution was filtered using a sterile syringe filter coupled to a filter unit with a low protein binding Durapore PVDF membrane (Merck Millipore) and the solution was poured into an amber tube to protect from light exposure.
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bioRxiv - Cell Biology 2024Quote: ... Standard immunostaining protocol was followed to stain for sarcomeric protein alpha-actinin and the coverslips were mounted on glass slides using fluoroshield (#F6182, Merck, Germany). Images were captured using the 60X oil immersion objective of the Olympus FV1000 confocal microscope (Olympus LifeScience ...
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bioRxiv - Biophysics 2023Quote: ... and the protein of interest was concentrated to 1-2mg/ml in a 50kDa molecular weight cut-off concentrator (Amicon Ultracel, Merck-Millipore). The resulting protein solution was subjected to size exclusion chromatography on a Superose 6 column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: Approximately 100 µg protein of each sample was separated by 12% SDS-PAGE and transferred to an Immobilon-PVDF membrane (Merck Millipore). The blot was blocked with 5% non-fat milk in 1× TBST (20 mM Tris base ...
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bioRxiv - Neuroscience 2024Quote: Proteins (30 μg) were separated on an 8% SDS-PAGE gel and blotted onto a PVDF membrane (GE10600029 Merck, Darmstadt, Germany). Membranes were blocked for 5 minutes with EveryBlot Blocking Buffer (12010020 Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... The purified protein sample was concentrated to 40 mg mL-1 using Amicon Ultra-15 3K MWCO Centrifugal Filter Unit (Merck Millipore) and subsequently diluted 1:4 with MilliQ water (yielding 10 mg mL-1 of protein in 1x crystal buffer (10mM Tris ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... were electrophoresed on SDS polyacrylamide gels ( NuPAGE™ 4-12% Bis-Tris Protein Gels; InvitrogenTM) and transferred to PVDF membranes (Millipore; Merck). Membranes were blocked in 2.5% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... of cell lysates by incubating lysates with 0.5-2.5 µg of antibodies and 20 µl of protein G–Sepharose (Merck, GE17-0618-01) at 4°C for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 30 μg of protein from each sample were subjected to SDS-PAGE electrophoresis and transferred to PVDF membrane (Merck Millipore, USA). Then the membrane was blocked in 5% w/v bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2019Quote: ... The final protein preparations were concentrated to 1 mM using Amicon® Ultra 10 or 3 kDa cut off centrifugal filters (Merck KGaA). Aliquots were frozen in liquid nitrogen and stored in −80 °C.
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bioRxiv - Cell Biology 2019Quote: ... Gels were then process for mass spectrometry or the proteins were transferred to the polyvinylidene difluoride membrane (Merck Millipore, Cat. No. IPVH00010) by Western Blotting (WB) ...
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bioRxiv - Biophysics 2021Quote: ... The peak fractions containing the MgtE TM domain protein were collected and concentrated to 10 mg/ml using an Amicon Ultra 50 K filter (Merck Millipore, USA) for crystallization.
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bioRxiv - Biophysics 2021Quote: ... and the flow-through fractions containing the MgtE TM domain protein were concentrated using an Amicon Ultra 50 K filter (Merck Millipore, USA). After concentration ...
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bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then equal amounts of total protein in each condition were resolved by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, ISEQ00010). The PVDF membrane was blocked with 1% (for anti-FLAG antibody ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation of FLAG-tagged Hfq was performed by incubating each sample with 75 µl pre-washed (3 x resuspended in 1 ml lysis buffer and centrifuged at 10,000 rpm for 1 min) protein A sepharose beads (Merck/Sigma-Aldrich #P6649) for 45 min at 4 °C (rocking) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.01% bromophenol blue) and loaded onto 10-12% SDS-polyacrylamide gels and proteins were transferred to nitrocellulose membranes (Merck-Millipore, Berlin, Germany). Afterwards ...
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bioRxiv - Cancer Biology 2022Quote: ... and the supernatant containing the sheared chromatin was pre-cleared with Protein G plus-Agarose beads (Cat No. IP04-1.5ML, Merck Millipore, Burlington, USA), which were pre-coated with bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2022Quote: ... The pellets were lysed for 1 h using cell protein extraction reagent (CW BioTech, CW0889, China) with protease inhibitor Cocktail set III (1 %, v/v, Merck, 535140, Germany), followed by centrifugation at 500×g for 10 min and the supernatants were collected ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: For phosphorylated proteins RIPA lysis buffer was further supplemented with PIC and 1X phosphatase inhibitor cocktail (Merck Millipore, cat no. 524629-1ML). A cocktail of phosphatase inhibitors (B-Glycerolphosphat (25mM) ...
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bioRxiv - Biochemistry 2021Quote: RNA immunoprecipitation (RIP) assays were conducted using the EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck Chemicals (Shanghai Co., Ltd). The anti-YTHDC1 antibody for RIP was purchased from Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were fractionated on 8% (w/v) sodium dodecyl sulfate polyacrylamide gels and transferred to Immobilon®-P PVDF Membrane (Merck, IPVH00010). Membranes were incubated in 5% (w/v ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The tissue was processed as previously reported [26] and proteins were loaded onto 10% polyacrylamide gels and transferred to PVDF sheets (Immobilion-P, MERCK, Burlington, USA). Membranes were blocked with BSA 5% and immunoblotted using primary antibodies for β-Tubulin ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein was concentrated overnight to a volume of 2 mL in a Vivaspin 20 Ultrafiltration Unit (5 kDa MWCO)(Merck, Darmstadt, Germany) and then loaded onto a HiLoadTM 26/60 Superdex TM 75 prep grade (GE Healthcare ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Biophysics 2020Quote: ... The fractions containing protein were combined and a buffer exchange and the protein were concentrated with a 30 K Amicon® Ultra Centrifugal Filter spin column (Merck KGaA) to the storage buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Physiology 2021Quote: ... For colocalization 1:100 anti-NBCe1 was incubated with 1:1,000 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07-623) or 1:200 anti-alpha tubulin (Santa Cruz cat#sc-5286 ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were then transferred by wet tank-blotting (100 V for 60 min) onto a polyvinylidene difluoride (PVDF) membrane (Merck, Darmstadt, Germany). The membrane was blocked with Roti®-Block (2 h at room temperature ...
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bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... at pH 2.0 with final protein concentration of 1.75 mg/mL (Sphβ preparation) or in 40% ethanol (v/v from ethanol absolute, Merck KGaA, Darmstadt, Germany), 0.25 M NaCl at pH 1.8 with final protein concentration of 5 mg/mL (Sphα preparation) ...