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Citations for Merck :
1251 - 1300 of 2518 citations for Pregnanediol 3 Glucuronide PDG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 5 and 10 μl of the lipid extract 2 cm from the bottom on aluminum-backed silica gel 60 TLC plates (cat#: 1.05553.0001; Merck) together with DOPG ...
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bioRxiv - Microbiology 2023Quote: ... a plastic TLC plate with a fixed layer of silica gel 60 (#1.05750.0001; Merck Millipore, Billerica, MA, USA) was employed ...
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bioRxiv - Neuroscience 2023Quote: ... scraped off the plates and lysed on ice for 30 min with 1X RIPA buffer (#20-188, Merck) supplemented with phosphatase (#P5726 ...
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bioRxiv - Biochemistry 2023Quote: The reaction mixtures (0.5, 1 or 2 µL) were spotted onto TLC Silica Gel 60 F254 plates (Merck). As for analysis of cyclization activity ...
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bioRxiv - Cell Biology 2023Quote: Cells were seeded in 96 well plates (6055302, Revvity) and incubated with 100 nM Bafilomycin A1 (Merck, B1793) or vehicle (DMSO ...
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bioRxiv - Plant Biology 2024Quote: ... using pre- treated silica gel plates (60 F254, 20 x 10 cm, 0.2 mm thickness; E. Merck, Germany). These TLC plates were modified by dipping in 0.2 M potassium dihydrogen orthophosphate solution (aqueous) ...
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bioRxiv - Neuroscience 2024Quote: ... The reactions were monitored by thin layer chromatography on silica gel plates (60 F254, Merck, Prague, Czech Republic), and the spots were visualized by ultraviolet light (254 nm) ...
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bioRxiv - Biochemistry 2024Quote: ... Each sample solution or marker (1 μl) was spotted onto a TLC plate (silica gel 60 F254, Merck). The TLC plates were developed appropriate times with 75% (v/v ...
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bioRxiv - Immunology 2024Quote: Retina and anterior cell suspensions were transferred into a 96-well 60-μm cell filter plate (Merck Millipore) and washed with 150µL of PBS ...
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bioRxiv - Immunology 2024Quote: ... cells after 12 days of IVS with OMPs pools were collected and plated in ELISpot plates (Merck Millipore) pre-coated with a monoclonal anti-IFN-γ mAb (clone 1-D1K purified MabTech ...
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bioRxiv - Biochemistry 2019Quote: ... concentrated and buffer exchanged (kinase NMR buffer, see below) by ultrafiltration (Amicon Ultra-0.5 ml, 3 kDa MWCO, Merck) for NMR analysis.
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bioRxiv - Microbiology 2021Quote: ... MilliQ water was produced in-house using a Milli-Q Integral 3 (LC-Pak polisher, Millipak express 40 filters, 0.22 µm, Merck) for a final organic content < 3ppb ...
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bioRxiv - Neuroscience 2020Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Molecular Biology 2019Quote: ... from an 18500g centrifugation (6 min at +4°C) were run through 3-kDa cut-off centrifugal filters (Amicon Ultra-0.5 ml, Merck) into pre-weighted collection tubes to remove remaining macromolecules ...
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bioRxiv - Cancer Biology 2020Quote: ... CM (and the same volume of SM as control) was then concentrated by 3 kDa Amicon filters (Merck-Millipore) to a final volume of 100 μl/mouse and stored at −20°C until usage ...
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bioRxiv - Biochemistry 2021Quote: ... 2/3 of the resulting supernatant (RIPA-insoluble fraction) was filtered through a nitrocellulose membrane (0.2 μM pore size, Merck) using a filter trap slot blot (Hoefer Scientific Instruments) ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were washed 3 times with deionized water (Milli-Q® IQ 7003 Ultrapure Lab Water System, Merck), and dehydrated by washing them in a graded series of ethanol ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μL of each sample was injected into a Chromolith FastGradient RP 18-e column (50 x 3 x 2 mm; monolithic silica with bimodal pore structure, macropores with 1.6mm diameter; Merck) protected by a precolumn (Gemini NX RP18 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Physiology 2022Quote: ... Metabolites were chromatographically separated in a SeQuant ZIC-cHILIC column (PEEK 100 x 2.1 mm, 3 μm particle size; Merck) at 30°C using a method consisting of a gradient running at 0.25 mL/min from 100% mobile phase B (9:1 acetonitrile:water containing 5 mM ammonium acetate pH 6.8 ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... This was followed by concentration with a centrifugal concentrator (Amicon Ultra-15 3 k device, Merck Millipore, Burlington, MA), and after supplementing with 10 mM DTT ...
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bioRxiv - Cancer Biology 2021Quote: ... and then concentrated using 15 ml Amicon spin filter cartridges (cutoff: 3 kDa, 10705884-Merck Millipore, Burlington, MA, USA) by serial addition of 10 ml of CM and centrifugation at 4000 x g until 1.5 ml were left ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated in primary antibodies solution (antibody diluted in PBS containing 3% goat serum, 0.01% Triton x-100 (Merck)) at 4°C over night ...
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bioRxiv - Biochemistry 2019Quote: ... using binding buffer and concentrated using a 3 kDa high molecular weight cut-off Amicon Ultra Centrifugal Filter (Merck). SDS-PAGE was used to assess the purity of untagged SbtB7001 ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μL of the bacteriocin sample was loaded onto the trap column at 3% (v/v) ACN (Merck, USA) containing 0.1% (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... the supernatant was applied to a centrifugation filter (Amicon Ultra 0.5 mL 3 K device; Merck KGaA, Darmstadt, Germany), and the fraction containing free amino acids was obtained by centrifugation (15,000 × g ...
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bioRxiv - Biochemistry 2021Quote: ... Appropriate fractions were concentrated to 3-0.7 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Biophysics 2021Quote: ... the dry lipid film was rehydrated with 3 mL of 0.2 M 8.5 pH Bicine (Merck Life Science, Norway) buffer (pH adjusted with NaOH (Merck Life Science ...
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bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
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bioRxiv - Developmental Biology 2022Quote: ... with 3 mg/mL of bovine serum albumin (BSA) and 100 µM of IBMX (Sigma-Aldrich, Merck, Darmstadt, Germany), and collected ...
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bioRxiv - Microbiology 2022Quote: ... and 10% ultra-pure water from Direct-Q 3 UV Water Purification System with LC-Pak Polisher (Merck KGaA) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Molecular Biology 2022Quote: All samples were centrifuged at 16,000 × g for 30 min and 400 µL supernatant was loaded on a 0.5 mL centrifugal ultrafiltration unit with 3 kDa cut-off membrane (Amicon, Merck) and concentrated down to 20 µL ...
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bioRxiv - Immunology 2022Quote: ... For intracellular cytokine labeling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (10μg/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2022Quote: ... The PDMS microstructures were then rinsed with 2:3 HEPES1/ethanol and with ultrapure water (Milli-Q, Merck-MilliPore). PVP (1.3 MDa ...
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bioRxiv - Neuroscience 2024Quote: ... for 180 min prior to washing and development in Streptavidin-tertiary reagent (1:500 in 2% BSA and 0.1% Triton [TBS]) conjugated to either Cyanine 3 (Cy3) (Merck) or Alexa Fluor 488 (Life technologies ...
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bioRxiv - Cell Biology 2023Quote: ... rinsed in running tap water for 3 min and stained with 0.02% Fast Green for 30 seconds (F7252, Merck), followed by 1% acetic acid for 30 seconds and Safranin’O solution for 45 min (S2255 ...
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bioRxiv - Biochemistry 2022Quote: ... The LE11-27 DNA was dialyzed against the same buffer and concentrated to ~ 2.5 mM (Vivaspin 500, 3 kDa MWCO, Merck). The complex was formed by mixing the protein and the DNA in a 2:1 protein-to-DNA ratio (final concentration of 800 µM and 400 µM ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in the presence or absence of aphidicolin 2μM for 3 hours together with cytochalasin B (3μg/ml, Merck Millipore) to block cytokinesis prior to fixation for immunofluorescence.
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bioRxiv - Biochemistry 2023Quote: ... HEK-293T cells were transfected at ∼40 % confluency using a 3:1 polyethylenimine (branched, average Mw ∼25,000 Da; Merck) to DNA ratio (30:10 μg ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... the mice were intranasally sensitized using 1 μg of 2′3′-cyclic GMP-AMP (cGAMP, 531889; Merck Darmstadt, Germany) and 1 μg of (HDM ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, Catalogue no. UFC500396) and centrifuged at 10,000 g for 20 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 24×50 mm high-precision coverslips (no. 1.5H; Marienfeld) were cleaned in Piranha solution (3:1, 98% H2SO4 (Merck):30% H2O2 (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... Liposomes were labeled before experiments with 1% of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (PE-Rhodamine) (Merck) at a concentration of 10µg/mL.
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bioRxiv - Cancer Biology 2023Quote: ... Japan) with a Discovery HS F5 column (2.1 mm i.d. × 150 mm, 3 μm particle size, Merck, Darmstadt, Germany) coupled with a Q Exactive instrument (PFPP-LC/MS ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked with Roti-Block (Carl Roth, Karlsruhe, Germany) or 3% bovine serum albumin (Merck KGaA, Darmstadt, Germany) in TRIS-TWEEN-buffered saline and incubated overnight with the primary antibodies as indicated ...
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bioRxiv - Bioengineering 2024Quote: ... The methanol-fixed samples were transferred to 3% glutaraldehyde solution (Agar scientific) in 0.1 M sodium phosphate buffer (Merck). Dehydration was performed with ascending ethanol series (30% ...
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bioRxiv - Neuroscience 2024Quote: ... 3 × 106 CGNs were mixed with 2 μg of a Mission shRNA vector for Snph (TRCN0000201959, Merck, Darmstadt, Germany) or pLKO.1-TRC-control (Addgene_#10879 ...
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bioRxiv - Microbiology 2024Quote: Infected RBCs were lysed with saponin (0.15%) in PBS and washed 3 times with protease inhibitor cocktail solution (PI, cOmplete Mini; Merck). Proteins were extracted using 1% Triton-X 100 (w/v ...