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Citations for Merck :
1251 - 1300 of 1307 citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... From the remaining nuclei RNA-IP was then undertaken with the MagnaRIP Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit protocol (17-700, Merck) from step I.3 ...
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bioRxiv - Immunology 2021Quote: ... was used for m6A immunoprecipitation (m6A-IP) using an m6A antibody (Synaptic, 202003) following the standard protocol of the Magna MeRIP m6A Kit (Merck Millipore). RNA was enriched through RNA Clean & Concentration-5 (Zymo Research ...
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bioRxiv - Plant Biology 2022Quote: A hole punch was used to obtain approximately 100 mg of leaf tissue from each sample (15 biological replicates per condition) and the total RNAs were extracted using the Spectrum Plant RNA kit (Merck, Spain). RNAs were quantified using the NanoDrop ND-1000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and carbonic anhydrase (29 kD) from a combination of two kits (GE Healthcare, catalogue No. 28-4038-41, Chicago, Illinois, IL, USA and MERCK, Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) histochemistry was performed using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore), according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2019Quote: ... Cells were fixed with 4% (w/v) paraformaldehyde and the proximity ligation assay (PLA) was conducted using the Duolink® In Situ Kit (Merck) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... To access the activation of both PI3K and MAPK signaling pathways the Muse™ PI3K/MAPK Dual Pathway Activation Kit (Merck) was used ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatants were then used to quantify the level of adiponectin using Mouse Adiponectin ELISA kit (Merck Millipore #EZMADP-6 K) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... were used to assess the difference in beta-secretase activity in each anatomical region using the Beta-Secretase Activity Fluorometric Assay Kit (Merck, MAK237).
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bioRxiv - Bioengineering 2022Quote: ... These oligonucleotides were used to create a phage library representing all oligopeptides using the T7Select 415-1 Cloning Kit (Merck Millipore). Immunoprecipitation of the library was performed in accordance with a previously published protocol (Harhala et al. ...
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bioRxiv - Plant Biology 2022Quote: The m6A enrichment experiment was performed as described previously with minor modifications (Dominissini et al., 2013) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck) was used following the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed using an Apop Tag Plus Peroxidase In Situ Apoptosis Kit (Merck Millipore) following the manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 2% paraformaldehyde for 10 min at room temperature and stained with the Hemacolor Rapid Staining Kit (Merck Millipore). Images were collected on BX61 upright microscope (Olympus ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... TNF-alpha and IL-10 in serum were determined using bead-based Multi-Plex kits (MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel 96-Well Plate Assay, Merck KGaA ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysate was prepared in 1X CHAPS Lysis Buffer and the assay was performed using TRAPeze™ RT Telomerase Detection Kit (Merck) per manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... The PCR product was run on an SDS gel and purified using the Agarose Gel DNA extraction kit from Merck (11696505001).
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bioRxiv - Microbiology 2023Quote: ... samples were taken at various time points and invertase activity was measured using the Invertase Activity Assay Kit (Merck, Darmstadt, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: The presence of T-DNA in transgenic plants was confirmed via extraction of genomic DNA and genotyping by PCR with the REDExtract-N-Amp™ Plant PCR-Kit (Merck, Darmstadt ...
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Multiple steps mediate ventricular layer attrition to form the adult mouse spinal cord central canalbioRxiv - Developmental Biology 2019Quote: ... TUNEL assay was performed on 20 μm thick cryosections using ApopTag® Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore, Cat. # S7100) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... Fixed cells were washed with cold PBS and centrifuged at 2000 x g for 5 minutes and stained using a Muse™ Cell Cycle Kit (Merck Millipore) for 30 minutes in dark conditions ...
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bioRxiv - Neuroscience 2020Quote: ... and the ultraView Universal Alkaline Phosphatase Red Detection Kit (Ventana Medical Systems) or by manual application of biotinylated secondary antibodies (Merck, RPN1001, RPN1004), peroxidase-conjugated avidin ...
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... After each PCR step the amplified regions were separated on agarose gel and purified using Illustra GFX™ PCR DNA and Gel Band Purification Kit (Merck KGaA).
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bioRxiv - Biochemistry 2021Quote: RNA immunoprecipitation (RIP) assays were conducted using the EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck Chemicals (Shanghai Co., Ltd). The anti-YTHDC1 antibody for RIP was purchased from Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Immunology 2021Quote: ... After each PCR step the amplified regions were separated on agarose gel and purified using Illustra GFX™ PCR DNA and Gel Band Purification Kit (Merck KGaA). The last oePCR step was performed to amplify the complete SARS-CoV-2S D19 sequence using primers carrying 15bp long 5’ overhangs homologous to the vector backbone ...
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bioRxiv - Physiology 2021Quote: ... and chemokine (C-X-C motif) ligand 1 (Cxcl1) were assessed using the MILLIPLEX-MAP kit Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Physiology 2020Quote: ... TUNEL staining was performed using a protocol modified from the ApopTag peroxidase in situ apoptosis detection kit (Merck-Millipore, Cat no. S7100). Paraffin sections of adipose tissues were prepared as above ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Annexin V/PI staining was performed with Muse® AnnexinV&Dead Cell Kit (Luminex, MCH100105) according to the manufacturer’s instructions and analyzed by Muse Cell Analyzer (Merck, Darmstadt, Germany). Western Blots for apoptotic markers ...
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bioRxiv - Microbiology 2022Quote: ... the medium was replaced with complete medium without phenol red and 10 μL of 5 mg/mL MTT solution (In vitro toxicology assay kit, MTT-based, TOX-1KT, Merck, Darmstadt, Germany) were added to each well ...
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bioRxiv - Plant Biology 2024Quote: ... according to manufactureres instruction using leaf RNA that had been extracted using the Spectrum Plant Total RNA Kit (Merck KGaA, Darmstadt, Germany). Constructs were amplified with the Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... apoptotic cell death in 1-day old zebrafish whole-mounts was detected according to a modification of the ApopTag® Fluorescein In Situ Apoptosis Detection Kit (Merck Millipore) protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted (from TNBC cell lines or cryopreserved tumor tissue) using the GenElute mammalian total RNA miniprep kit (Sigma/Merck, RTN350-1KT) according to manufacturer’s instructions with an additional step of DNA digestion using the On-Column DNAse I digestion set according to manufacturer’s instructions (Sigma/Merck ...
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bioRxiv - Microbiology 2023Quote: ... assays were used to detect single- and double-stranded DNA nicks and fragmentation via the ApopTag® Fluorescein Direct In Situ Apoptosis Detection Kit (Merck Group). Testes dissected from 5-day old males were fixed in 4% formaldehyde ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the cell lysates was measured using the BCA assay and insulin levels were quantified by radioimmunoassay (rat insulin RIA kit, Merck, Darmstadt, Germany).
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bioRxiv - Plant Biology 2023Quote: ... was used to synthesize DIG-labeled sense and antisense RNA probes using DIG RNA labeling kit (SP6/T7) (Merck KGaA, Darmstadt, Germany). The DIG-labeled RNA probes were purified using ProbeQuant G-50 Micro Colums (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... supernatant from the cells was collected and used to quantify LDH release using the LDH cytotoxicity detection kit (Roche, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and the number of neutrophils that migrated through the membrane was counted on a flow cytometer using Muse Count & Viability Kit (#LUMIMCH100102, Merck, NJ, USA) according to the producer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... These pX330-mC plasmid DNAs and donor DNA plasmid were isolated using FastGene Plasmid Mini kit (Nippon Genetics, Tokyo, Japan) and filtered using MILLEX-GV® 0.22 μm filter unit (Merck Millipore, Darmstadt, Germany) for microinjection ...
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bioRxiv - Microbiology 2021Quote: ... Serum cytokine profiling targeting 22 cytokines/chemokines was performed by Public Health England using a 22 -plex customized Luminex kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed for 1 min in 4% formaldehyde and stained for AP activity using the Alkaline Phosphatase Detection Kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Biochemistry 2019Quote: The detection of apoptotic cells was based on the nick-end (TUNEL) labelling method using the ApopTag® in situ detection kit (S7100, Merck Millipore, USA), according to the manufacturing protocol.
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bioRxiv - Physiology 2021Quote: ... terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed using an ApopTag® In Situ Peroxidase Detection Kit (S7100, Merck Millipore, USA) according to the manufacturer’s recommendations and as previously described (36) ...
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bioRxiv - Cancer Biology 2022Quote: ... stained with Muse Cell Cycle Reagent (The Muse® Cell Cycle Kit, Luminex, MCH100106) and analyzed with Muse Cell Analyzer (Merck, Darmstadt, Germany). Staining and cell cycle analysis procedure is detailed in Supplementary Information.
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... The animals used in our experiments were genotyped for expression of the transgene by quantitative PCR (qPCR) using the KAPA Mouse genotyping kit (Merck, Cat# MGKITKB-KK7301), with primer sequence (5’-3’ ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Final concentration was measured by Qubit™ RNA BR Assay Kit (Thermo Fisher Scientific. RNA integrity was assessed by gel electrophoresis with 1:1 formamide (Merck Millipore) and 1 µl of loading dye on 1.5% agarose gel ...