Labshake search
Citations for Merck :
1151 - 1200 of 1742 citations for Trans 4 Hydroxy L Proline Rabbit Polyclonal Conjugated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... Mowiol® 4-88 used for mounting medium was purchased from Merck (Kenilworth, MJ, USA). Cell culture reagents – Ham’s-F12 ...
-
bioRxiv - Biochemistry 2021Quote: ... The purified complex I was concentrated using an Amicon Ultra-4 100K centrifugal filter (Merck) to 0.5 mg mL-1 (w/v) ...
-
bioRxiv - Biochemistry 2020Quote: ... 0.625 mM TBTA (Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine) (Merck Millipore), and 6.25 mM CuSO4 (Merck Millipore) ...
-
bioRxiv - Biochemistry 2022Quote: ... After the standard procedure of Ni-NTA purification (Ni-NTA beads, Merck, cat. 70666-4) and Sumo protease cleavage (in-house production ...
-
bioRxiv - Biochemistry 2022Quote: ... 4 % glycerol) freshly supplemented with 5µg/ml Poly(dI-dC) (Merck Life Science cat. P4929) and 0.5 mM DTT ...
-
bioRxiv - Biochemistry 2021Quote: ... The active fraction was concentrated using a 50 kDa cutoff filter Amicon Ultra-4 (Merck), and of 20 μL was mixed with 2 μL of 5.9 mM coelenterazine and 278 μL of 20 mM Bis-Tris-HCl (pH 7.0) ...
-
Nerve pathology is prevented by linker proteins in mouse models for LAMA2-related muscular dystrophybioRxiv - Neuroscience 2022Quote: ... General histology was assessed after tissue fixation with 4% paraformaldehyde by H&E staining (Merck) or Picro Sirius Red stain (Direct Red 80 (Sigma ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... using a LiChroCART 250 × 4 mm LiChrospher 100 RP 18 column (Merck KGaA, Darmstadt, Germany). The mobile phase was a mixture of water and acetonitrile (70:30 ...
-
bioRxiv - Microbiology 2021Quote: ... Antibodies used for ChIP were anti-H3K4me3 (Merck Millipore, 04–745, 4 μl per ChIP), anti-H3K27me3 (Merck Millipore ...
-
bioRxiv - Pathology 2020Quote: ... the harvested tissues were immediately fixed in 4% (w/v) paraformaldehyde (PFA; Merck, Darmstadt, Germany) for 24 hours ...
-
bioRxiv - Cell Biology 2021Quote: ... After treatment for the indicated time the cells were fixed in 4% paraformaldehyde (Merck, 100496,) and permeabilized with cold methanol at −20°C for 2 min or 0.1% TX-100 in PBS for 5 min at room temperature followed by blocking with 0.12% glycine (Fisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... 21,000 x g at 4°C and sterile-filtered using a 0.45µm PVDF-membrane (Merck).
-
bioRxiv - Molecular Biology 2022Quote: ... Rad54 was concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore).
-
bioRxiv - Cell Biology 2023Quote: ... sections were rehydrated and stained with haematoxylin (Fluka) for 4 min and with eosin (Merck) for 2 min ...
-
bioRxiv - Cell Biology 2023Quote: ... and 0.1% X-Gal (from a 4% X-Gal solution in dimethylformamide, Merck Life science). Buffer was passed through a 0.2 micron syringe filter before X-Gal was added ...
-
bioRxiv - Developmental Biology 2023Quote: ... and left overnight at 4°C in blocking solution containing 3% Donkey Serum (D9663, Merck) and 0.03% Sodium Azide (40-2000-01 ...
-
bioRxiv - Cancer Biology 2023Quote: ... membranes were stained with Ponceau S and subsequently blocked in 4% BSA (A8022, Sigma/Merck) in 1x PBS-Tween (137 mM NACl ...
-
bioRxiv - Immunology 2023Quote: ... cells were fixed for 30 min at 4°C with 0.4 % paraformaldehyde (PFA, Merck KGaA) and permeabilized by washing twice with permeabilization buffer (PBS containing 2 % FBS and 0.1 % saponin (Sigma)) ...
-
bioRxiv - Genetics 2023Quote: ... individual fibers were transferred onto a 4-well chamber slide (Nunc Lab-Tek, Merck, GmbH) containing HBSS with 2.5μM FM1-43 dye (Molecular Probes ...
-
bioRxiv - Neuroscience 2023Quote: For conditioning the odors 4-MCH (1:250, Merck, Darmstadt, Germany,CAS #589-91-3) and 3-OCT (1:167 ...
-
bioRxiv - Biochemistry 2023Quote: ... After the standard Ni-NTA purification process using Ni-NTA beads (Merck, cat. 70666-4) and subsequent Sumo protease digestion (concentration of sumo protease at 1:200) ...
-
bioRxiv - Neuroscience 2023Quote: ... and transcardially perfused with 0.9% NaCl followed by 4% paraformaldehyde (PFA, Sigma, Merck, Germany, P6148). The brains were harvested and post-fixed overnight in 4% PFA at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... permeabilized with 0.1% saponin in PBS and incubated with 4 μg/ml Hoechst 33342 (Merck) for 10 min at room temp ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were then fixed and permeabilized using a combination of 4% paraformaldehyde (Sigma-Aldrich/Merck) and 0.1% Triton X-100 (Sigma-Aldrich/Merck) ...
-
bioRxiv - Biochemistry 2023Quote: ... samples were concentrated at 3,500 rpm and 4°C using Amicon Ultra centrifugal units (Merck).
-
bioRxiv - Synthetic Biology 2023Quote: ... 500 µl 0.2 µg/ml 4’,6’-diamidino-2-phenylindole (DAPI, Merck, catalog no. D9542) in PBS was added for 15 min at RT ...
-
bioRxiv - Plant Biology 2024Quote: ... 4 mM phosphoenolpyruvate (PEP)) as well as 1.2 U pyruvate kinase (P1506; Sigma-Aldrich/Merck) were added to convert ADP and PEP to ATP and pyruvate ...
-
bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were applied overnight at 4°C (monoclonal anti–IdU 1:4000; SAB3701448, Merck). Sections were incubated in biotinylated secondary antibodies (Jackson ImmunoResearch Labs ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... determined by means of quantitative NMR using TraceCERT® ethyl 4-(dimethylamino)benzoate from Merck as an internal calibrant [48 ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatant was then concentrated through an Amicon Ultra-4 Filter Unit (Merck, cat#UFC8100214). The validation of the cytoplasm fraction was performed by western blot using antibodies against markers for nucleus (H2A) ...
-
bioRxiv - Biochemistry 2024Quote: ... Fractions containing ELAC2 were concentrated using Amicon Ultra-4 30K Centrifugal Filter Devices (Merck Millipore), aliquoted ...
-
bioRxiv - Immunology 2024Quote: ... cells were treated for 4 h with 5 mg/ml Brefeldin A (Merck, Darmstadt, Germany) after 140 h incubation time ...
-
bioRxiv - Microbiology 2019Quote: ... Under these conditions nitrite was not detectable with a colorimetric test with a lower detection limit of 2 mg/L (MQuant test stripes, Merck, Darmstadt, Germany). Growth conditions and operation of the bioreactor containing ANME-2d archaea enriched from an Italian paddy field soil are described by Vaksmaa et al. ...
-
bioRxiv - Microbiology 2020Quote: ... The residue was then resuspended in 100 µl methanol and one µl of each sample analysed by thin layer chromatography (TLC) using silica gel 60 F254 (Merck Millipore, Germany) with chloroform-acetic acid-ethanol (90:5:10 [vol/vol] ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tissues were directly immersed in CUBIC-L solution (10 wt % N-Butyldiethanolamine [Tokyo Chemical Industry, B0725], 10 wt % Triton X-100 [Merck, 1.08603.1000] and 80 wt % MilliQ water) ...
-
bioRxiv - Plant Biology 2022Quote: ... We mixed 0.1 μl template cDNA and 500 nM of each primer (Supplementary Table S1) with KAPA SYBR Fast qPCR Master Mix (Merck, Darmstadt, Germany). Each reaction comprised an initial denaturation step for 3 min at 95 °C ...
-
bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich, Merck Life Science), 1 g/L monosodium glutamate (Sigma Aldrich ...
-
bioRxiv - Biochemistry 2023Quote: ... dehydrated with 100 μL acetonitrile and rehydrated with 25 μL trypsin solution, containing 5 ng/μL trypsin (cat # 37283, SERVA Electrophoresis GmbH, Heidelberg) in 3 mmol/L ammoniumbicarbonate (cat # 09830, Merck KGaA, Darmstadt). After 4 h incubation at 37 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... All plastic and glassware used to culture MPAs was coated with poly-L-lysine (PLL; 4µg/mL; catalogue # P1274, Merck Life Sciences (UK)) ...
-
bioRxiv - Microbiology 2024Quote: ... and 100 µL of undiluted sample and each dilution were plated on Eosin Methylene Blue Agar (Biomaxima, Lublin, Poland) and MacConkey Agar (Biomaxima, Lublin, Poland) supplemented with imipenem (16 mg/L) (Merck, Darmstadt, Germany) to search for carbapenem resistance phenotype ...
-
bioRxiv - Microbiology 2023Quote: ... The chlorine concentrate solution was prepared by addition of a NaOCl tablet (B-Care Chemicals, Belgium) to 1 L ultrapure water (Milli-Q, Merck Millipore, Germany). The amount of free chlorine was quantified with the Pocket Colorimeter II (Hach,Belgium).
-
bioRxiv - Developmental Biology 2021Quote: ... and incubated with FITC (Fluorescein isothiocyanate) conjugated secondary antibody (0.5 μg/ml of Goat Anti-Mouse IgG-FITC in 5%BSA) (GeNei, Merck, USA) (Cat.no ...
-
bioRxiv - Biochemistry 2021Quote: ... Anti-phosphotyrosine antibody conjugated to agarose beads (clone 4G10; 16-101) and anti-phosphotyrosine antibody (Clone 4G10; 05-321) were obtained from Merck Millipore (Darmstadt ...
-
bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected with a horseradish peroxidase (HRP)-conjugated secondary antibody (1:3000, #170-6515, Biorad or #12-349, Merck Millipore ...
-
bioRxiv - Biophysics 2019Quote: ... the DNA-conjugated GNPs were separated from an excess amount of oligonucleotide by 100 kDa MWCO filters (Amicon Ultra, MERCK) with TE buffer added to the filter ...
-
bioRxiv - Cancer Biology 2020Quote: ... The glutathione-S-transferases (GST) fusion proteins were expressed in Escherichia coli and purified with glutathione-conjugated agarose beads (Merck) by standard procedures ...
-
bioRxiv - Neuroscience 2020Quote: ... slices were incubated with mouse monoclonal Alexa Fluor-488 conjugated antibody against NeuN (1:200, MAB377X, Merck Millipore, MA, USA) or the rabbit polyclonal primary antibody against DISC1 (1:250 ...
-
bioRxiv - Neuroscience 2023Quote: ... Plates were washed three times with 0.1% PBST and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary antibody (Cat No. NA934, Merck Millipore) diluted 1:1,000 in 0.05% PBST ...
-
bioRxiv - Microbiology 2023Quote: ... binding of equine antibodies was detected by incubation for 1 hour with protein A conjugated to peroxidase (GE) and visualized with chemiluminescence reagent (ECL, Merck). Images were obtained with an Odyssey LI-COR instrument ...
-
bioRxiv - Neuroscience 2023Quote: ... fluorescent visualization of the nonisotopic signals used anti-digoxigenin antibodies conjugated to horseradish peroxidase (anti-digoxigenin-POD; Fab fragment; 1/200; Merck). This was followed by the deposition of biotin tyramide (1:1000 ...