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Citations for Merck :
1151 - 1200 of 1623 citations for Recombinant Rabbit CCL5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... to 20 μg of mitochondrial protein and run with Tris-MOPS running buffer (#PCG3003; Sigma-Aldrich/Merck) on a 4-20% precast Trupage gel (#PCG2012-10EA ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were concentrated where necessary using Amicon filter devices with appropriate molecular weight cut-off (Millipore; Merck).
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd., Ireland). 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter ...
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bioRxiv - Plant Biology 2024Quote: ... and proteins were concentrated using a 30 kDa Amicon Ultra-4 Centrifugal Filter Unit (Merck Milipore; www.merckmillipore.com). Protein concentration was determined employing the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... The fractions with target proteins were pooled and concentrated using an Amicon Ultra 3K NMWL (Merck Millipore). The sample was ultracentrifuged (45,000 rpm x 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Target protein-containing fractions were combined and concentrated using an Amicon Ultra-4 50 kDa filter (Merck) operated at 7,500 x g and 4 °C ...
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bioRxiv - Physiology 2023Quote: ... 20 μg of total protein were transferred onto centrifugal filter units (Microcon-30 kDa; Merck, Darmstadt, Germany) and washed with a buffer containing 8 M urea and 100 mM Tris (pH 8.5) ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated by ultrafiltration using an Amicon Ultra-0.5 mL (10 kDa cutoff, Merck Millipore) at 14,000 × g and 4°C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were then sterilised by passing the eluate through a 0.2 μm filter (Merck Millipore), then concentrated using a 3kDa Vivaspin 20 centrifugal concentrator column (Sartorius ...
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bioRxiv - Immunology 2023Quote: ... Medium was collected after this period and filtered through 0.45μm ultra-low protein binding filter (Merck Millipore) to remove cell debris ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing purified protein were united and concentrated on Amicon-Ultra-15 10 K filtration units (Merck-Millipore ...
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bioRxiv - Biophysics 2023Quote: ... The TEV-cleaved protein was then buffer exchanged using a 30 kDa MWCO centrifugal filter (Amicon, Merck) into buffer containing no imidazole and 50 mM KCl (25 mM K·HEPES ...
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bioRxiv - Systems Biology 2023Quote: ... The separated proteins were transferred in a semi-dry system onto PVDF membranes (Immobilon P, Merck Millipore) using transfer buffer (192 mM glycine ...
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bioRxiv - Neuroscience 2023Quote: ... Protein-containing fractions were pooled and concentrated on an Amicon 3 kDa MWCO spin concentrator (Merck Millipore). After another IMAC purification step using Ni-NTA resin ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated using Amicon Ultra-15 centrifugal filters (100 kDa cut-off, Merck-Millipore). After treatment with 1 mM diamide for 15 min to completely oxidize the protein ...
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bioRxiv - Molecular Biology 2023Quote: ... PsC23 protein-containing fraction was collected and imidazole was removed by 10K centrifugal filter (Merck Millipore, Germany) using buffer (20 mM Tris-Cl ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were separated by reducing Tris-glycine SDS-PAGE and transferred to polyvinylidene difluoride (PVDF, Merck Millipore) membranes followed by enhanced chemiluminescence analysis (Advansta ...
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bioRxiv - Biochemistry 2023Quote: ... Buffer exchange to 1xHBS and protein concentration was conducted by ultrafiltration (Amicon Ultra 10 kDa MWCO, Merck).
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated overnight with 30 μL ChIP-grade Protein G agarose beads (Merck Millipore, Germany) and 10 µg DNMT1 ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE gels were used for protein separation followed by transfer onto PVDF membrane (Merck, Cat. # IPFL00010). Blots were blocked with 5% BSA at room temperature for 1 h and incubated with the following primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal quantities of protein were separated on SDS-PAGE gels and transferred to the PVDF membranes (Merck), which were then blocked with 1 % nonfat skim milk in TBS-T for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to a Immobilion®-P Transfer Membrane (0.45 µm pore size, Cat#: IPVH00010, Merck) and blocked for 1h at RT in 1xTris Buffered Saline Tween (TBST ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S200 (16/600 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated by SDS-PAGE (7.5%–10% PAG) and transferred onto Immobilon-P membranes (Merck Millipore). Membranes were immunoblotted with the respective antibodies as follows ...
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-PAGE and then transferred to 0.45 μm PVDF membrane (IPVH00010, Merck Millipore). To keep the amounts of protein equally ...
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bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions containing target protein were desalted and concentrated on Amicon Ultra 10K centrifugal filter units (Merck Millipore), and the final protein preparation was stored in PBS containing 0.05% Triton X-100 at 4°C.
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bioRxiv - Molecular Biology 2024Quote: ... diluted and precleared with 30 μL protein A/G agarose beads (Merck-Millipore, 16-157/16-201) for 1h at 4°C ...
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bioRxiv - Systems Biology 2024Quote: ... 20 ug of proteins were separated by 10% SDS-PAGE and transferred onto a PVDF membrane (Merck). Membranes were blocked in 5% milk or bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... The eluted protein solution was in turn transferred to an ultrafiltration column (Amicon Ultra-15, Merck Millipore) and concentrated by centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... The protein samples were separated on 10% SDS-polyacrylamide gels and transferred to PVDF membrane (IPFL00005, Merck) for semi-dry transfer ...
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bioRxiv - Biochemistry 2024Quote: ... We loaded 10 µL of protein mixed in a 1:1 ratio with Laemmli 2x Concentrate (Merck). The gel was ran for 35min at 200V with Tris/Glycine/SDS buffer (BIORAD ...
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bioRxiv - Microbiology 2020Quote: ... The following secondary antibodies were used: sheep α-rabbit horseradish peroxidase (HRP) (catalogue number AP510P, 1/10,000; Merck) and goat α-mouse HRP (STAR117P ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed using 4% paraformaldehyde and stained with antibodies against ZO-1 (rabbit polyclonal, #617300, Merck-Millipore) and Nestin (mouse monoclonal ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP (AP182P, Merck Millipore) or donkey anti-mouse IgG HRP (AP192P ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were incubated with a 50,000-fold-diluted HRP-labelled anti-mouse or rabbit IgG antibody (Merck) at room temperature for 2 hours or more ...
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bioRxiv - Neuroscience 2024Quote: ... The antibodies used were: rabbit anti-tyrosine hydroxylase antibody (1:1000, AB152, Merck Life Science SLU, Darmstadt, Germany), chicken anti-GFP (1:500 ...
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The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretionbioRxiv - Neuroscience 2024Quote: ... They were then incubated with rabbit anti-cholecystokinin (26–33) (CCK-8) antibody (diluted 1:1000, Merck, C2581) for 16 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated with primary antibody rabbit anti-c-fos (1:1000, Merck Millipore, Burlington, United States) in TBS (5% BSA ...
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bioRxiv - Molecular Biology 2022Quote: ... Immunodetection and FISH were conducted as described previously 78 using the following antibodies: rabbit H3K27me3 (#07-449 - Merck) diluted 1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with a biotinylated donkey antibody to rabbit IgG (10 μg/ml; AP182B, Merck Millipore) and then with Alexa488-conjugated streptavidin (2.5 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: PLAs were carried out using a Duolink® in situ red starter kit mouse/rabbit (Merck, Dorset, UK). HUVECs and spread platelets were fixed ...
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bioRxiv - Cell Biology 2023Quote: ... LatB-actin was prepared by incubating rabbit skeletal muscle actin with a tenfold molar excess of LatB (Merck) overnight at +4°C ...
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bioRxiv - Immunology 2021Quote: ... Conjugated HEL-OVA solution was further purified from unconjugated proteins using a 50 kDa Amicon filter tube (Merck).
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bioRxiv - Immunology 2021Quote: ... Proteins were electrophoretically separated and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Merck Millipore, Burlington, MA, USA). Blocking treatment was performed using the PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing the target protein were concentrated to 5 ml with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was loaded onto a 320 ml HiLoad Superdex 75 (GE Healthcare Life Science ...
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bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... antibody complexes were purified by incubation of lysate with Protein A Sepharose® beads (Merck, GE17-5138-01) for 3h rotating at 4°C ...