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Citations for Merck :
1151 - 1200 of 2650 citations for Rat Insulin Like Growth Factor Binding Protein 5 IGFBP5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and the FLAG-tagged proteins were immunoprecipitated with anti-FLAG agarose (A2220, MERCK). Input samples were collected as described above.
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bioRxiv - Biochemistry 2024Quote: ... Immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Fluor-Chem M (Proteinsimple) ...
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bioRxiv - Molecular Biology 2024Quote: ... FLAG-tagged proteins were detected using M2 anti-FLAG antibody (mouse, F1084, Merck) followed and fluorescent Anti-Mouse Alexa Fluor™ Plus 800 ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in protein resuspension buffer (150 mM NaCl (Merck, USA), 5 mM EDTA (Merck ...
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bioRxiv - Molecular Biology 2024Quote: ... protein pellets were resuspended in 50 µl of 1.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
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bioRxiv - Developmental Biology 2023Quote: ... and separated proteins were then transferred to PVDF membranes (polyvinylidene difluoride, Merck Millipore). After treatments with a blocking reagent (Blocking One solution ...
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bioRxiv - Physiology 2023Quote: ... a specific inhibitor targeting the alpha subunit of the Gi/o protein (Merck); U73122 ...
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bioRxiv - Biochemistry 2023Quote: ... The 139H2 was purified with Protein G Sepharose 4 Fast Flow beads (Merck), washed with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... protein lysates were prepared using 1X RIPA buffer containing protease (#539131, Merck Millipore) and phosphatase (#524629 ...
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bioRxiv - Molecular Biology 2023Quote: Proteins separated by SDS-PAGE were transferred to Immobilon-P membranes (Merck Millipore) utilizing a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins separated by SDS-PAGE were transferred to Immobilon-P membranes (Merck Millipore). Three major bands on the membrane were cut and subjected to N-terminal Edman sequencing by Hokkaido System Science Co. ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Amersham, MERCK). Membranes were blocked with Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in 50 µl of 2.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
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bioRxiv - Biochemistry 2023Quote: ... Then the protein was concentrated with centrifugal concentrators (UFC901096, Merck Millipore, Cork, Ireland), and loaded onto a SuperoseTM 6 Increase 10/300 GL gel-filtration column (29- 0915-96 ...
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bioRxiv - Cell Biology 2023Quote: Wet blot transfer was used to transfer proteins to a PVDF membrane (MERCK). Intercept blocking buffer (927-60001 ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated with first and secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved in SDS-PAGE gels and electrotransferred onto nitrocellulose membranes (Merck). After incubation with the appropriated primary antibody ...
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bioRxiv - Microbiology 2023Quote: ... Then the protein sample was concentrated using an Amicon Ultra centrifugal tube (Merck) with a buffer change to phosphorylation buffer 2X (100 mM Tris pH 8 ...
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bioRxiv - Plant Biology 2023Quote: ... after concentrating the protein (Amicon® Ultra MWCO 30 kDa, Merck, Darmstadt, Germany), a second GST-tag purification was performed and the peak fractions were pooled and concentrated (Amicon® Ultra MWCO 10 kDa ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were blotted on the Immobilon-P PVDF 0.45 µm (MERCK, Darmstadt, Germany). PVDF membranes were blocked using the PVDF Blocking Reagent for Can Get Signal (TOYOBO) ...
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bioRxiv - Microbiology 2023Quote: ... and the protein concentration was determined by bicinchoninic acid (BCA) assay (Merck Millipore) and by UV-visible spectroscopy using an ε of 24 mM⁻¹·cm⁻¹ at 280 nm ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were concentrated by centrifugal filters (Amicon Ultra 10K, Merck Millipore Ltd.). Protein concentrations were determined by a Pierce™ BCA Protein Assay Kit (Thermo Fischer Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 µM Rho-associated protein kinase (ROCK) inhibitor (Merck Millipore, SCM-075). On day 3 ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were filtered using an Ultrafree 0.22 μm centrifugal filter (Merck Millipore) to remove any aggregation ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to 0.45 µm PVDF membranes (Merck Millipore, cat.no. IPVH00010). After blocking unspecific binding sites of the membrane at RT for 1 h with 5 % skim milk powder in TBS-T ...
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bioRxiv - Cell Biology 2023Quote: Caco-2 cells were co-transfected with eSpCas9-GFP protein (Merck, cat #ECAS9GFPPR) and sygRNA (Merck ...
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bioRxiv - Physiology 2023Quote: ... and protein concentration in the supernatant was determined by BCA assay (Merck B9643). Finally ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Plant Biology 2024Quote: ... Protein pellets were resuspended in 50 μl of 1.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred onto a 0.45 µm PVDF Immobilon-P membrane (Millipore, Merck) using the Pierce Power Blotter (Thermo Fisher) ...
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bioRxiv - Biochemistry 2019Quote: ... and csm5 were cloned into pACYCDuet-1 (5’-NcoI, 3’-XhoI; Novagen, Merck Millipore), csm4 was cloned into pEHisTEV (5’-NcoI ...
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bioRxiv - Cell Biology 2020Quote: ... at a final concentration of 5 μg/ml or Latrunculin B (428020-1MG, Merck) at a final concentration of 5 μM in M2 medium supplemented with dbcAMP ...
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bioRxiv - Biochemistry 2020Quote: ... using a ZIC-pHILIC column (150 mm × 4.6 mm, 5 μm column, Merck Sequant). The column was maintained at 30 °C and samples were eluted with a linear gradient of solvent A (20 mM ammonium carbonate in water ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.1% β-mercaptoethanol (Cat N° 31350010)) supplemented with 5 μM Y-27632 (Merck Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked using 5% milk powder in TBS / 0.1% Tween20 (Merck, 655205-250ML). All antibodies are listed in Supplementary Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... After blocking for one hour in PBST with 5% normal goat serum (Merck G9023) and 0.1% BSA ...
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bioRxiv - Microbiology 2019Quote: ... 5 ml of cultures were transferred onto Durapore PVDF filter membranes (Merck, Darmstadt, Germany) with a pore size of 0.45 µm by suction ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were extracted in 70% ethanol plus 5% formic acid (Merck-Millipore) twice for 20 min with permanent shaking ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... UPLC setup consisted ZIC-pHILIC column (SeQuant; 150 mm × 2.1 mm, 5 μm; Merck). 5 μl of cells extracts were injected and the compounds were separated using a mobile phase gradient of 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... with a Chromolith® RP-18 endcapped 5-3 guard cartridges (Merck, Darmstadt, Germany), operated under a flow rate of 0.3 mL/min ...
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bioRxiv - Biochemistry 2022Quote: ... and both His6 and GST tags were cleaved with 5-10 unit thrombin (Merck) per milligram of protein for 24 h at 4°C and subjected to the SEC using the superdex 200 10/30 GL column ...
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bioRxiv - Biochemistry 2022Quote: ... 10 mM DTT) and loaded onto a MonoQ 5/50 column (Sigma-Aldrich, Merck). Bound proteins were eluted using a 30 CV linear gradient (68 mM to 1 M (NH4)2SO4) ...
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bioRxiv - Biochemistry 2022Quote: ... using a ZIC-pHILIC (150 mm × 4.6 mm, 5-μm particle) column (Merck Sequant). A 15-min elution gradient was used (80% solvent A to 20% solvent B) ...
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bioRxiv - Bioengineering 2020Quote: ... using a ZIC-pHILIC column (150 mm × 4.6 mm, 5 μm. column, Merck Sequant). The datasets were processed using XCMS (peak picking) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Paraffin sections (5 μm) were stained with hematoxylin/eosin (H&E; Merck, Darmstadt, Germany). For immunohistochemistry (IHC) ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM DTT) using a 10 kDa cut-off Amicon Ultra-15 concentrator (Merck), and frozen at –80 °C until use ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were incubated for 5 minutes with autofluorescence eliminator reagent (Merck Millipore, 2160) to reduce background ...