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Citations for Merck :
1101 - 1150 of 1357 citations for Rat TGF beta 2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... TEER was evaluated with the Millicell ERS-2 Voltohmmeter at the beginning and at the end of the assay (Merck-Millipore, Overijse, Belgium).
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bioRxiv - Genetics 2021Quote: ... Cells were differentiated by LIF withdrawal in DMEM supplemented with 10% FBS and 0.1 mM ß-Mercaptoethanol at a density of 2×104 cells/cm2 on Fibronectin-coated dishes (Merck, 10 µg/ml).
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bioRxiv - Developmental Biology 2021Quote: We mated Exosc10+/+ or Exosc10+/− females with stud Exosc10+/− male mice and recovered two-cell stage (gestational day 2; GD2) embryos by flushing approximately 0.5 ml of EmbryoMax Advanced KSOM medium (Merck-Sigma, MR-101-D) into the oviduct ...
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bioRxiv - Cell Biology 2021Quote: ... BHK-2P maintained either in Glasgow’s Minimal Essential Medium (GMEM) with 10% FBS (“line #2”) or adapted to the ACFM Cellvento™ BHK200 (Merck KGaA, Darmstadt, Germany) in two different processes (lines “#4” and “#5”) ...
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bioRxiv - Microbiology 2022Quote: ... medium was added to the apical side 30min prior to measurement with a chopstick electrode connected to a Volt-Ohm-meter (Millicell® ERS-2, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were pooled and concentrated to a final concentration of ∼2 mg/ml using an Amicon Ultra-4 cellulose centrifugal filter unit (Merck Millipore, Cat# UFC810024).
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bioRxiv - Microbiology 2023Quote: ... 2× overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS/DMEM media and 0.6% (v/v) methylcellulose (Merck Life science, Cat: M0512) to achieve a semi-solid overlay ...
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bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... until reaching the desired staining contrast, and cleared in two xylene baths (2 min, 20 min) before being coverslipped with an entellan-xylene solution (Merck KGaA, Darmstadr, Germany).
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were pooled and concentrated to 500 μl using Amicon Ultra-15 10K MWCO centrifugal filters (Merck Millipore UFC901008; final LDAO = 2%) and further purified by size-exclusion chromatography as described for human VDAC constructs but with 20 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged twice a week using 2 mM ethylenediaminetetraacetic acid in phosphate buffer saline (EDTA/PBS; Thermo Fisher Scientific, Waltham, USA; Merck, Darmstadt, Germany) adding 10 µM Y27632 (Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... injections 3x a week (consecutive days) during 2 weeks of 25 mg/kg 3PO or 75 mg/kg 3PO or dimethyl sulfoxide (DMSO) (Sigma-Aldrich, Merck, Overijse, Belgium) as control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
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bioRxiv - Neuroscience 2023Quote: ... We replenished the medium the next day and started selection against replicating non-neuronal cells on day two using neuronal medium containing 2 μM Cytosine β-D- arabinofuranoside (AraC) (Merck Life Sciences). We gradually diluted AraC out by replacing half of the medium with fresh neuronal medium every other day ...
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bioRxiv - Bioengineering 2023Quote: ... Fractions 7 to 10 which were subsequently identified as the particle fractions (PF) were pooled to a volume of 2 ml and concentrated using an Amicon Ultra 100 kDa centrifugal filter (Merck Millipore, MA, USA), made from regenerated cellulose ...
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bioRxiv - Biochemistry 2024Quote: Mixing of lysozyme crystals (7×2 mm; grown by batch crystallisation) with 25 mM sulfanilic acid azochromotrop (SAA, Merck, lmax 505–510 nm), a highly absorbing red dye ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA was extracted using the GenElute™ Mammalian Total RNA Miniprep Kit (Merck) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The DNA was extracted using a DNA purification kit (Merck Millipore, Darmstadt, Germany). Anti-RNA polymerase II antibody and normal mouse IgG were used as specific antibodies and the negative control ...
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bioRxiv - Developmental Biology 2020Quote: ... Genomic DNA was extracted using GenElute™ Mammalian Genomic DNA Miniprep Kit (Merck), following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Microbiology 2019Quote: ... The nitrate content was measured using Spectroquant Nitrate Test Kit HC707906 (Merck, Germany) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Blots were developed with the ECL kit (Thermo Fischer Scientific or Merck Millipore) and x-ray film and quantified using ImageLab.
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bioRxiv - Cell Biology 2020Quote: ... Blots were developed with the ECL kit (Thermo Fischer Scientific or Merck Millipore) and x-ray films.
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bioRxiv - Cancer Biology 2021Quote: Intracellular protein was extracted from cells using the NucBuster Protein Extraction Kit (Merck) and quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: Gram staining of bacterial strains was performed using a Gram staining kit (Merck). Strain Ha5T cultured in LMG medium 404 broth for 24 hours was determined for cellular morphology using scanning electron microscope (FEI Quanta FEG 650) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
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bioRxiv - Immunology 2022Quote: ... MCP-3 and RANTES were quantified using a multiplex kit (MILLIPLEX MAP; Merck, Burlington ...
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bioRxiv - Microbiology 2022Quote: ... Peptide content in each fraction was verified using QuantiPro BCA Assay Kit (Merck). Zn content was determined using PAR against a standard curve.
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bioRxiv - Cancer Biology 2022Quote: ... which was performed using the EZ-Nuclear RIP Kit (17-701, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: SecA ATPase activity assay was done using malachite green kit (MAK307, Merck/Sigma). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... protein concentration was quantified using a Sigma-Aldrich BCA protein assay kit (Merck). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... the cytoskeleton) were extracted using the “ProteoExtract Subcellular Proteome Extraction kit” (Calbiochem/ MERCK) according to the supplier’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The PLA was performed using the Duolink Proximity Ligation Assay Kit Red (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... aeruginosa NCTC 11442 was extracted using the GenElute Bacterial Genomic DNA kit (Merck). Plasmid backbones and inserts for cloning were amplified from the purified chromosomal DNA using Q5 High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were finally embedded in epoxy resin (Epoxy embedding medium kit, Merck KGaA) that was then baked for 24 hours at 60 °C ...
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bioRxiv - Cell Biology 2023Quote: ... stained using the ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore) according to the manufacturer’s instructions and counterstained with haematoxylin (Fluka ...
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bioRxiv - Cell Biology 2023Quote: ... PBMCs were pre-stained with the PKH67 Green Fluorescent Cell Linker Kit (Merck) as a ground truth label ...
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bioRxiv - Neuroscience 2023Quote: ... Active (acylated) ghrelin concentrations were determined using the EZGRA-88K kit (Merck, Germany) in duplicates (total analytical CV at 488 pg/ml 12%).
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bioRxiv - Cell Biology 2023Quote: Saporin was labelled with an ATTO 550 protein labelling kit (Merck, 51146-1KT).
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bioRxiv - Neuroscience 2023Quote: ... Active (acylated) ghrelin concentrations were determined using the EZGRA-88K kit (Merck, Germany) in duplicates (total analytical CV at 488 pg/ml 12%).
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bioRxiv - Neuroscience 2023Quote: ... the DIG probe was detected using an HNPP fluorescent detection kit (11758888001, Merck). The FITC probe was detected as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... enhanced chemiluminescence assays were performed using the Millipore kit (Merck-Millipore, Stockholm, Sweden). The antibodies used are listed in the Supplementary Table S1 ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Microbiology 2020Quote: The crude ethylacetate extract of ALR-2 was purified via column chromatography using a LiChroprep RP-18 (15-25 μm) silica gel column (Merck, EMD Millipore Corporation, USA) and chloroform:methanol (ratio 4:1 ...
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bioRxiv - Biochemistry 2021Quote: ... modifying on previous reported conditions for the assay of MjRibK.[2] The reaction mixture was incubated at 37°C overnight and visualized using fluoresce-based thin layer chromatography (TLC) (Merck Silica gel 60 F254) using the long wavelength (365 nm ...
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bioRxiv - Microbiology 2022Quote: ... we used the same approaches as described above and spotted the 5 μl of bacteria on LB agar plates containing 2% of Skim Milk Powder for microbiology (Sigma-Aldrich/Merck KGaA, Darmstadt, Germany). Plates were incubated at 25°C and monitored for hemolytic and protease activities after 1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions containing the desired protein were pooled and concentrated to 2 ml in Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... 48 and 72 hours) of exposure to simulated solar radiation was performed by using 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) (Sigma Aldrich-Merck KGaA, Darmstadtm Germany) solubilized in ethanol ...
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bioRxiv - Cancer Biology 2021Quote: ... and cell count and viability were determined with the Muse Count & Viability Kit (Merck) on the Muse Cell Analyser (Merck) ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...