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Citations for Merck :
1101 - 1150 of 1171 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... deparaffination was followed by 1-hour incubation in room temperature with the primary antibody (monoclonal rat, MAB386; Merck Millipore, Darmstadt, Germany) diluted 1:800 in dilution buffer (Agilent Dako ...
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bioRxiv - Cell Biology 2020Quote: ... GFP (1:2000) (Faix et al., 2001) or mouse monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000; #CB1001-500UG, Merck (Darmstadt, Germany)) and anti-fascin antibody 5E2 (undiluted hybridoma supernatant) ...
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bioRxiv - Neuroscience 2021Quote: ... PBS rehydrated tissue was then blocked using 10% normal horse serum in 0.2% Triton X-100 in PBS for ∼1 h and then primary antibodies specific for S100 (Merck, S2532, 1:200) TUJ1 (Synaptic systems ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2021Quote: ... the slices were processed with 1% H2O2 in phosphate buffer to deactivate the intrinsic peroxidase and then incubated with an anti-c-Fos antibody (1:10,000, rabbit; Merck KGaA, Darmstadt, Germany) in 10% normal goat serum in phosphate-buffered saline with 0.3% Triton X-100 (PBS-X ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were then reprobed with monoclonal anti-β-actin-peroxidase clone AC-15 antibody (a3854, Merck, Saint Louis, MO, dilution 1:5000) in TBS/T for 90 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2020Quote: ... and probed with 1:1000 diluted primary anti-Ago2 rat monoclonal antibody clone 11A9 (Millipore Sigma/ Merck KGaA MABE 253, Darmstadt, Germany), 1:25000 diluted primary mouse monoclonal anti-beta actin antibody (AC-15 ...
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bioRxiv - Neuroscience 2020Quote: ... and the ultraView Universal Alkaline Phosphatase Red Detection Kit (Ventana Medical Systems) or by manual application of biotinylated secondary antibodies (Merck, RPN1001, RPN1004), peroxidase-conjugated avidin ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min each at RT and incubated with secondary antibodies in TPBS-TS-1% for 4h at 4°C (Alexa Fluor 546 anti-Mouse (Merck, A-11018), Alexa Fluor 488 anti-goat (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunofluorescence staining of the DNA double-strand-break marker γH2AX [32] was performed using a JBW301 mouse monoclonal anti-γH2AX antibody (Merck, Darmstadt, Germany) and an Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% (20mM Tris and 500mM NaCl pH 7.5) and incubated overnight at 4°C with the primary antibody: anti-GluN1 (1:1000; Merck KGaA, Darmstadt, Germany) and anti-GluN2A monoclonal antibody (1:1000 ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were probed with anti-GFP at a dilution of 1:3000 of the antibody conjugated to a peroxidase (SAB2702198, Merck KGaA, Germany) in the blocking buffer overnight at 4 °C on a rocker ...
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bioRxiv - Neuroscience 2020Quote: ... 0.2% Tween-20 in PBS) for 1 h and then incubated with a rabbit antibody against the extracellular domain of CSF1R (Merck, 06-174,) or a mouse anti-HA tag antibody (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were also probed with either a mouse monoclonal antibody against GAPDH or a mouse monoclonal antibody against beta-actin (dilutions of 1:10,000 and 1:1000; Sigma-Aldrich/Merck group, USA) and with the IRDye® 680CW secondary antibody from Eurobio Ingen (926-32220 ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 and incubated overnight at 4°C followed by an secondary anti-rabbit HRP conjugated antibody (12-348, Merck Millipore, Germany) diluted 1:5000 in PBST and incubated for 1 h at RT ...
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bioRxiv - Cell Biology 2024Quote: ... IHC staining was performed using Ki67 and cleaved caspase 3 antibodies and the 3,3’-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride (Merck Millipore, Darmstadt, Germany) as described previously30.
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.
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bioRxiv - Cell Biology 2023Quote: Anti-LUZP1 antibodies were generated by injecting 200 μg of GST-LUZP1 (aa 262-427) mixed with Freund adjuvant (Merck, Darmstadt, Germany) into a rabbit every two weeks totally four times ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
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bioRxiv - Bioengineering 2024Quote: ... The scaffold was then rinsed three times with PBS and incubated with Alexa Fluor™ 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) and CF™ 633 conjugated secondary donkey anti-rat IgG antibody (1:100 ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was rinsed with TBS buffer and incubated with the primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) (1:2,500 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then permeabilized by addition of ice-cold 70% ethanol and stained with an anti-H3S10ph antibody (Merck-Millipore, 05-1336) diluted 1:1000 for 1 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Infection foci were visualised using the appropriate secondary antibody coupled with horseradish peroxidase (HRP; 1:5000, Sheep Anti-Mouse IgG A5906, Sigma-Aldrich, Merck, Germany) and TrueBlueTM chemical (SeraCare ...
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bioRxiv - Microbiology 2024Quote: ... Actin was detected with monoclonal mouse antibody coupled with horseradish peroxidase (HRP; 1:75,000; A3854, Sigma-Aldrich, Merck, Saint-Quentin Fallavier, France). Membranes were exposed to the appropriate peroxidase-conjugated secondary antibodies (1:5000 goat anti-guineapig IgG A108P or 1:10000 sheep anti-mouse IgG A5906 ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was incubated overnight with primary antibodies (anti-PSD95, MA1-046, ThermoFischer Scientific, USA, 1:1000) and anti-stargazin Rabbit anti-stargazin (TARP γ2) (AB9876, Merck, 1:1000), washed with TBST ...
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bioRxiv - Immunology 2024Quote: ... were co-incubated with TCR-T cells (2,000-10,000 cTCR-positive cells/well) in IFN-γ antibody-coated Multiscreen HTS plates (Merck-Millipore, Darmstadt, Germany) overnight (16-20h) ...
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bioRxiv - Immunology 2024Quote: ... The membrane was washed with PBST before adding the secondary HRP-coupled antibodies at 1:3000 dilution (α-rabbit, sigma) or at 1:3000 dilution (α-rat, Merck). Blots were visualised with ECL (Amersham ...
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bioRxiv - Bioengineering 2021Quote: Three sender area squares (3 × 3 mm) were put into a 24-well containing 300 μL of anti-His antibody (Merck, Germany, NOVG70796-3) (10 μg mL−1 in DPBS ...
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bioRxiv - Plant Biology 2021Quote: ... the sections were incubated in 1× PBS containing 1% (1:100 dilution) mouse monoclonal anti-Flag M2 primary antibody (Merck, cat. no. F1804) and 0.1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2022Quote: ... Incubation with appropriate secondary HRP-labelled antibody (less than 1h) was followed by detection with Immobilon western chemiluminescent HRP substrate (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were prepared and subjected to western analysis as described previously (49) using antibodies to the following: α-tubulin (T9026, Sigma-Aldrich, Merck, Inc.), ATF4 (11815 ...
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bioRxiv - Physiology 2023Quote: ... they were incubated in their respective secondary antibodies for 1 h at room temperature (goat anti-rabbit, IgG, HRP conjugated; Merck Millipore (1: 5000) in 5% milk-TBST ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% BSA TBS-T or 5% skim milk TBS-T and incubated overnight at 4°C with the following primary antibodies diluted in 5 % BSA or 5% skim milk dissolved in TBS-T: pSer293-PDH (1:1,000) (Merck Millipore, Cat. no. ABS204), PDH (1:1,000 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue sections from the in vivo experiment were incubated with a rabbit primary antibody for GluR2/3 (Merck Millipore; AB1506; 1:200) overnight and then with a secondary goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Immunology 2023Quote: ... The embryos were subsequently incubated overnight at 4 °C in solution containing Cy3-conjugated α-smooth muscle actin monoclonal antibody (1:100, C6198; Merck, Darmstadt, Germany), Alexa647-Phalloidin (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... we incubated the nuclei suspension on ice for 30 min with an anti-NeuN antibody (Alexa647-conjugate, clone A60, Merck, cat. no. MAB377) diluted 1:500 in 1X PBS/10 mM EDTA pH 7.4 followed by filtration of the nuclei suspension through a 30 µm pre-separation filter (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked 1h in 10% donkey serum in PBST and incubated overnight with primary antibodies for anti-GFAP (1:250, Merck Life Science, Hpa056030) and anti-MAP2 (1:200 ...
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bioRxiv - Genomics 2024Quote: ... with the following primary antibodies: goat anti-IL1RN (RD Systems Cat # AF280NA) in 1:1000 in blocking solution and mouse anti-β actin (MERCK Cat # A1978) and rabbit anti-vinculin (Cell Signaling Cat #18799 ...