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Citations for Merck :
1101 - 1150 of 1458 citations for 6 Thien 2 ylpyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The PDMS particles were generated by vortex-mixing a solution of 1 g PDMS (10:1 w/w, base/curing agent) dispersed in 10ml of 2%w/v poly(ethylene glycol) monooleate (Merck Chemicals GmbH, Germany) aqueous solution ...
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bioRxiv - Microbiology 2022Quote: ... were sequentially filtered by vacuum through hydrophilic polycarbonate filters with a pore size of 20 µm (47 mm; Sterlitech, Kent, WA, US) and then 2 µm (Isopore, 47 mm; Merck Millipore, Cork, Ireland). Filters were immediately plunged into liquid nitrogen and stored at −80°C until further processing ...
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bioRxiv - Cell Biology 2019Quote: ... The percentage of COX-2 positive cells was quantified on 5.103 dissociated UC-hMSCs using a Guava® easyCyte Flow Cytometer (Merck Millipore, Guyancourt, France). The results were compared to the fluorescent intensity distribution obtained by image analysis.
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bioRxiv - Microbiology 2019Quote: ... TEER was evaluated with the Millicell ERS-2 Voltohmmeter at the beginning and at the end of the assay (Merck-Millipore, Overijse, Belgium).
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bioRxiv - Genetics 2021Quote: ... Cells were differentiated by LIF withdrawal in DMEM supplemented with 10% FBS and 0.1 mM ß-Mercaptoethanol at a density of 2×104 cells/cm2 on Fibronectin-coated dishes (Merck, 10 µg/ml).
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bioRxiv - Developmental Biology 2021Quote: We mated Exosc10+/+ or Exosc10+/− females with stud Exosc10+/− male mice and recovered two-cell stage (gestational day 2; GD2) embryos by flushing approximately 0.5 ml of EmbryoMax Advanced KSOM medium (Merck-Sigma, MR-101-D) into the oviduct ...
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bioRxiv - Cell Biology 2021Quote: ... BHK-2P maintained either in Glasgow’s Minimal Essential Medium (GMEM) with 10% FBS (“line #2”) or adapted to the ACFM Cellvento™ BHK200 (Merck KGaA, Darmstadt, Germany) in two different processes (lines “#4” and “#5”) ...
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bioRxiv - Microbiology 2022Quote: ... medium was added to the apical side 30min prior to measurement with a chopstick electrode connected to a Volt-Ohm-meter (Millicell® ERS-2, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were pooled and concentrated to a final concentration of ∼2 mg/ml using an Amicon Ultra-4 cellulose centrifugal filter unit (Merck Millipore, Cat# UFC810024).
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bioRxiv - Microbiology 2023Quote: ... 2× overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS/DMEM media and 0.6% (v/v) methylcellulose (Merck Life science, Cat: M0512) to achieve a semi-solid overlay ...
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bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... until reaching the desired staining contrast, and cleared in two xylene baths (2 min, 20 min) before being coverslipped with an entellan-xylene solution (Merck KGaA, Darmstadr, Germany).
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were pooled and concentrated to 500 μl using Amicon Ultra-15 10K MWCO centrifugal filters (Merck Millipore UFC901008; final LDAO = 2%) and further purified by size-exclusion chromatography as described for human VDAC constructs but with 20 mM HEPES pH 7.6 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... injections 3x a week (consecutive days) during 2 weeks of 25 mg/kg 3PO or 75 mg/kg 3PO or dimethyl sulfoxide (DMSO) (Sigma-Aldrich, Merck, Overijse, Belgium) as control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
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bioRxiv - Neuroscience 2023Quote: ... We replenished the medium the next day and started selection against replicating non-neuronal cells on day two using neuronal medium containing 2 μM Cytosine β-D- arabinofuranoside (AraC) (Merck Life Sciences). We gradually diluted AraC out by replacing half of the medium with fresh neuronal medium every other day ...
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bioRxiv - Bioengineering 2023Quote: ... Fractions 7 to 10 which were subsequently identified as the particle fractions (PF) were pooled to a volume of 2 ml and concentrated using an Amicon Ultra 100 kDa centrifugal filter (Merck Millipore, MA, USA), made from regenerated cellulose ...
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bioRxiv - Systems Biology 2020Quote: ... as well as valproic acid sodium salt (VPA; 1mM, Merck Millipore) and db-cAMP (0.5 mM ...
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bioRxiv - Biophysics 2019Quote: ... and 10 µM N-1-naphthylphthalamic acid (NPA, Sigma-Aldrich/Merck) for 3 weeks ...
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bioRxiv - Physiology 2019Quote: ... and then washed in 0.05 M maleic acid (Merck, Darmstadt, Germany) three times for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Solvent A consisted of 0.1 M acetic acid (Merck, Kenilworth, NJ) in Milli-Q (Millipore ...
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bioRxiv - Plant Biology 2020Quote: ... supplemented with 135 μl of 0.83 M perchloric acid (HClO4, Merck), vortexed and centrifuged for 15 min at 4 °C and 13000 rpm using a table centrifuge (19330 G) ...
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bioRxiv - Cell Biology 2021Quote: CI976 was used as a lysophosphatidyl acid acyltransferase inhibitor (Merck # C3743). CI976 intermediate stock solution was made at 10mM in dimethylsulfoxide (DMSO) ...
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bioRxiv - Microbiology 2021Quote: ... was equilibrated with 5 mM sulphuric acid (H2SO4) (Titrisol, Merck, Germany) in water at 55 °C ...
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bioRxiv - Biophysics 2020Quote: The solution of peptides was then acidified with Trifluoroacetic acid (Merck) to a final concentration of 1% and a pH value of < 2 ...
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bioRxiv - Biochemistry 2022Quote: ... 1% (w/v) fatty acid-free bovine serum albumin (BSA, Merck) and 1x EDTA-free cOmplete™ protease inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 μL 0.32 mg/mL pepsin in 0.5M acetic acid (Merck) was added and again left overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 200μM oleic acid complexed to albumin (Merck) and 1μg/ml BODIPY-558/568-C12 (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... in 10 mM ammonium formate (1ml formic acid (26N) (Merck, 1.11670.1000), 3ml ammonia (13N ...
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bioRxiv - Microbiology 2023Quote: ... The elution fraction was precipitated with 10% trichloroacetic acid (TCA) (Merck) and analyzed by Western blotting for detection of 3xFLAG-tagged IsdF using an anti-FLAG M2 antibody (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.2% formic acid (FA) and 50% acetonitrile (ACN) (Merck Darmstadt, Germany). Eluted peptides were dry-evaporated and resuspended in 10% FA solution for subsequent tandem mass spectrometry analysis (LC-MS/MS) ...
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bioRxiv - Immunology 2023Quote: ... in 10 mM ammonium formate (1ml formic acid (26N) (Merck, 1.11670.1000), 3ml ammonia (13N ...
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bioRxiv - Biochemistry 2022Quote: ... supplemented with 1% MEM Non-Essential Amino Acid Solution (Merck: M7145), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... 10% acetic acid solution and staining with 0.25% Coomassie-blue (Merck) in 50% methanol ...
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bioRxiv - Biochemistry 2023Quote: ... Mercury chloride and Benzene-1,3,5-tricarboxylic acid were obtained from Merck Chemical Company ...
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bioRxiv - Cell Biology 2023Quote: ... all sections were incubated in 1 % phosphotungstic acid (#100583, Merck Millipore) for 10 min at RT ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Soluble proteins were then dosed using the Bicinchoninic Acid Kit (Merck) and diluted in Laemmli protein sample buffer ...
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bioRxiv - Biophysics 2024Quote: ... Sodium hydroxide (99%) and hydrochloric acid (99%) were purchased from Merck, GermanyTh.Geyer and CHEM-SOLUTE ...
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bioRxiv - Cancer Biology 2024Quote: ... fatty acids were annotated by comparison to FAME Standard Mixture (Merck Life Science UK Limited ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μg of protein was added to each well and run in Boric acid-Tris buffer (192 mM Boric acid, Merck; 1 mM EDTA, Merck; 0.1% SDS, to pH 7.6 with Tris) at 25 mA for 1.5 h ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Microbiology 2020Quote: The crude ethylacetate extract of ALR-2 was purified via column chromatography using a LiChroprep RP-18 (15-25 μm) silica gel column (Merck, EMD Millipore Corporation, USA) and chloroform:methanol (ratio 4:1 ...
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bioRxiv - Biochemistry 2021Quote: ... modifying on previous reported conditions for the assay of MjRibK.[2] The reaction mixture was incubated at 37°C overnight and visualized using fluoresce-based thin layer chromatography (TLC) (Merck Silica gel 60 F254) using the long wavelength (365 nm ...
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bioRxiv - Microbiology 2022Quote: ... we used the same approaches as described above and spotted the 5 μl of bacteria on LB agar plates containing 2% of Skim Milk Powder for microbiology (Sigma-Aldrich/Merck KGaA, Darmstadt, Germany). Plates were incubated at 25°C and monitored for hemolytic and protease activities after 1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions containing the desired protein were pooled and concentrated to 2 ml in Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... 48 and 72 hours) of exposure to simulated solar radiation was performed by using 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) (Sigma Aldrich-Merck KGaA, Darmstadtm Germany) solubilized in ethanol ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were then postfixed with 0.8% K3Fe(CN)6 (Merck), 1% OsO4 (Serva ...
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bioRxiv - Immunology 2019Quote: ... the inhibitor 6-amino-4–4-phenoxyphenylethylamino-quinazoline (Merck Millipore, USA) was reconstituted in DMSO at a stock concentration of 1mM and administered at a final concentration of 10nM on Days 0 and 4 ...
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bioRxiv - Cell Biology 2021Quote: ... 6 μM of mitochondrial pyruvate transporter inhibitor UK9055 (Merck, cat. 5048170001) were added to test if mitochondria have the capacity to utilize other substrates than pyruvate ...