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Citations for Merck :
1101 - 1150 of 5322 citations for 6 9 Methano 4 1 benzoxazepin 2 3H one octahydro 3 methyl 3 α 5a bta 6 bta 9 bta 9a bta 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... anti-α-Tubulin mouse monoclonal (DM1A) antibodies (#T6199) (Merck); anti-DAPK3 polyclonal antibodies (#PA5-90193 ...
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bioRxiv - Microbiology 2023Quote: ... and α-FLAG antibody coupled to agarose beads (Merck) for ChIP-seq of RpoC-FLAG and FLAG-GafZ.
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bioRxiv - Plant Biology 2024Quote: ... and goat α-rabbit IgG (Merck, Catalogue No.: AP307F) antibodies at dilutions of 1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... and one 11μM nylon filter (NY1102500, Merck Millipore). The filtrate was centrifuged at 1500 rcf for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were blocked and incubated with primary antibodies against bornavirus P (1:500) and α-tubulin (Merck, Darmstadt, Germany), followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 2.5 µg mL-1 α-factor grown at 25°C (Cat. No. T6901, Merck). After 90 min ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μL lipid extract was injected on a LiChroCART 250–4 LiChrospher® Si 60 (5 μm) (Merck) maintained at 25 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 500 μM of the protease inhibitor 4-(2-aminoethyl)-benzolsulfonyfluorid hydrochloride (BioChemica) and 5 U/mL-benzonase (Merck) were added (final concentrations) ...
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bioRxiv - Cancer Biology 2021Quote: ... Percentage of GFP- or mCherry-positive cells was measured every three days from day 3 to day 18 post-infection using Guava® easyCyte™ Flow Cytometer (Merck Millipore). The percentage of fluorescent cells in the population on each day was divided by their percentage on day three to calculate fold-change corresponding to the effect of a given sgRNA on cell proliferation.
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bioRxiv - Biochemistry 2019Quote: ... The fractions corresponding to the single peak were pooled and concentrated using an Amicon Ultra −15 centrifugal filter (3 kDa, Merck Millipore Ltd. Ireland). Copper-(I ...
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bioRxiv - Biochemistry 2020Quote: ... and the buffer was exchanged to buffer B without imidazole using 3 kDa Amicon® centrifugal filters (0.5 ml size) (both from Merck KGaA, Darmstadt, Germany).
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bioRxiv - Biophysics 2020Quote: ... at different concentrations (20 mM, 200 mM and 500 mM) and concentrated using centrifugal ultrafiltration devices (3 kDa cut-off) (Merck India Pvt. Ltd.). This was followed by Gel filtration chromatography using Superdex 200 Increase 10/300 GL column (GE Lifesciences ...
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bioRxiv - Cancer Biology 2019Quote: ... the plasmid construct of β-galactosidase was co-expressed in equal amounts in wells and expression was measured in the presence of the ONPG substrate (Merck, 369-07-3). The reaction was stopped with 1M Tris-Cl (pH 11 ...
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bioRxiv - Biochemistry 2021Quote: ... 500 µL filter sterilized supernatant was concentrated to 100 µL using Amicon ultra centrifugal filter units with 3 000 Da cut-off (Merck Millipore, Cork Ireland) and washed three times in 10 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... mice were gavaged with 0.2 ml of Fluorescein Isothiocyanate-Dextran (FITC-Dextran; 3–5 kDa; cat. no. FD4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The flow through and wash fractions were concentrated to 3-5 mg/mL using Amicon ultrafiltrators (10 kDa MWCO, Merck Millipore, Darmstadt, Germany). All fractions were combined and dialyzed 2x over night at 4 °C against 5 L 10 % (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... The fixing solution was aspirated and wells washed 3 times in a washing buffer composed of DPBS containing 0.5% of BSA (Merck Life Science, Gillingham, UK). The washing buffer was left in the wells for 10 minutes following the last wash ...
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bioRxiv - Systems Biology 2023Quote: ... the 2x 50 μl were pooled and then split in 3 x 33 μl which were each dialyzed against 50 mL of MilliQ H2O (Merck Millipore, Darmstadt, Germany) as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer of the eluted fractions containing 6xHis-HULla was replaced by buffer G (50 mM Tris pH 8, 200 mM NaCl) in Amicon® Ultra – 3 kD Centrifugal Filters (Merck KGaA, Germany) and resulting solution was injected in a heparin column ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 0.1% Tween-20 (PBS-T) and blocked using PBS supplemented with 4% w/v skimmed milk powder (PBSM-4%; Merck Millipore) for 1h at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: ... 1% streptomycin and 2 mM of Glutamax and 0.1 μg·ml−1 GDNF (#SRP3200, Merck) (Vyas et al. ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM EGTA) and fixated for 20 min with 4% paraformaldehyde (Merck). Then ...
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bioRxiv - Biochemistry 2023Quote: ... 400 μg of AtLEGβ or papain were inhibited with 0.5 mM or 5 mM S-methyl methanethiosulfonate (MMTS, Merck), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... and then embedded in BMM resin (butyl methacrylate, methyl methacrylate, 0.5% benzoyl ethyl ether with 10 mM DTT (Merck)) at -20°C under UV light for polymerization ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-alpha tubulin (Merck-SIGMA, clone B-5-1-2) was used as a loading control at 1:10000.
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bioRxiv - Cell Biology 2024Quote: ... 1 nM β-estradiol and 2 μg/mL insulin (Merck). Any red blood and unattached cells were removed by media change within 18 hours ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor at 30°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Microbiology 2023Quote: ... α-M2 FLAG antibodies coupled to sepharose beads (Merck, UK) were employed for ChIP-seq of Cada2-FLAG and RpoC-FLAG ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:1 (v:v) with water containing 4 mM MgCl2 and benzonase (Merck #70746, 250 U/µl), and incubated for 15 min at RT to digest nucleic acids ...
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bioRxiv - Microbiology 2023Quote: ... 100 ml of the cell suspension at an OD660 of 0.6 was harvested by centrifugation at 5,000×g for 10 min at 4°C and resuspended in 2 ml of BugBuster (Merck), and 50 μg/ml lysozyme and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
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bioRxiv - Biochemistry 2023Quote: ... was performed on 4 and 2.5 mg protein lysates for timsTOF optimization and dual-probe analyses respectively in 2 M urea (Merck) in 1× 50 mM HEPES (pH 7.5) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of 50 mM EDTA was added to the reaction mixture and unchelated radioactive copper ion was removed by centrifugation using Amicon® Ultra centrifugal filters (regenerated cellulose, 3 kDa) (Merck Millipore, Billericia, MA). The radioactivity of 64Cu complexed NOTA-POx was measured in gamma counter prior to micelle preparation.
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bioRxiv - Microbiology 2020Quote: ... spores were collected from 5-day-old cultures NO3 medium (0.17% yeast nitrogen base, 3% sucrose, 100mM KNO3) by filtering through miracloth (Merck; pore size of 22–25μm). Spores were centrifuged ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HPLC purification of the (S,S)-MLN-4760 was based on the comparison with the commercially available compound (MLN-4760; MW: 428.31; Merck, CAS N° 305335-31-3). The isolation of the desired (S,S)-diastereoisomers of the respective fluorinated MLN-4760 derivatives was based on the assumption that the HPLC elution sequence of the diastereoisomers would be identical to that of the MLN-4760 lead compound.
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bioRxiv - Microbiology 2023Quote: Aliquots of 3-12 μl of cell-free supernatant or enzymatic reactions were spotted onto silica coated plates (Merck, TLC Silica gel 60 F254) and resolved in butanol:acetic acid:water (2:1:1) ...
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bioRxiv - Immunology 2024Quote: ... the wellbeing of the fish was followed at least once a day and fish exhibiting symptoms, signs of pain or discomfort were euthanised with an overdose of a Tricaine anaesthetic (Ethyl 3-aminobenzoate methanesulfonate, (Merck, Kenilworth, New Jersey, USA)) ...
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bioRxiv - Neuroscience 2022Quote: ... and one 11 µM nylon filter (NY1102500, Merck Millipore). The filtrate was then centrifuged at 1500 RCF for 15 minutes at 4 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with one round of Tri-Reagent® (Merck Millipore). cDNA synthesis was conducted according to manufacturer’s protocol with 500 ng of RNA ...
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bioRxiv - Microbiology 2024Quote: ... diluted 1:100 in PBS with 1% BSA and 2% Triton™ X-100 (Merck Life Science UK Limited ...
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bioRxiv - Biochemistry 2023Quote: ... the reduced cysteine residues were blocked employing a 10 mM solution of S-methyl methanethiosulfonate (MMTS) (Merck KGaA, Darmstadt, Germany) at room temperature for 10 minutes ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Adult mosquitoes were provided ad libitum access to 10percent sugar solution (8percent sucrose +2percent glucose +0.05percent methyl paraben +5percent vitamin syrup (Polybion SF Complete, Merck Ltd.)) ...
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bioRxiv - Neuroscience 2020Quote: ... blocked again and incubated overnight at 4°C with DAPI (1:2000) (Merck) and anti-rabbit AlexaFluor-488 (1:500 ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor grown at 25°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Cell Biology 2019Quote: ... 500µl of this pellet suspension was added to a tube in which 2.5 mg of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, Avanti Polar lipids, Merck, Sigma-Aldrich, l’Isle d’Abeau, France) in chloroform had previously been evaporated ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Birds from the anxiogenic group received 2.5 mg/kg of β-CCM (β-carboline-3-carboxylic acid-N-methylamide [FG 7142], Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) dissolved in DSMO (Dimethyl Sulfoxide suitable for HPLC ...
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bioRxiv - Neuroscience 2022Quote: ... Six rats received an infusion of 1.5 μL (n = 4) or 3.0 μL (n = 2) of 10% glycerol (Merck, Darmstadt, Germany) in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then stained with a 1X crystal violet solution for 20 minutes at RT (2% crystal violet, Sigma; 20% ethanol; 4% formaldehyde, Merck). Plates were washed with clear water and plaques were counted manually ...
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bioRxiv - Genomics 2023Quote: ... 4 % DDT and 2 % clothianidin-impregnated papers prepared in acetone and Dow Corning 556 Silicone Fluid (BHD/Merck, Hesse, Germany). Flies overexpressing CYP6Z2 were also exposed to the primary product of hydrolysis of pyrethroids ...
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bioRxiv - Genomics 2024Quote: ... About 2 mL of supernatants were then transferred to ultrafiltration tubes (Amicon® Ultra-4 Centrifugal Filter Unit 10K, Merck). 2 mL of TE buffer was added ...