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Citations for Merck :
1051 - 1100 of 1226 citations for Recombinant Human Serpin Peptidase Inhibitor Clade B ovalbumin Member 9 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 150 mM NaCl in the presence of Protease inhibitor Cocktail Set III (Calbiochem®, Merck, Darmstadt, Germany) and the phosphatase inhibitor sodium orthovanadate (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: Treated BMDM cells were lysed with RIPA buffer (supplied with cOmplete™ Protease Inhibitor Cocktail, Merck#11697498001), and protein concentration was determined by Bradford assay ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 7.9) on ice for 1 h in the presence of EDTA-free Protease Inhibitor Cocktail (Merck). Lysates were cleared by centrifugation at 20,000 g for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 7.9) on ice for 1 h in the presence of EDTA-free Protease Inhibitor Cocktail (Merck) and cleared by centrifugation at 20,000 g for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... 2 ml EDTA tubes were prepared with 100μl protease inhibitor (Pefabloc® SC Plus, Merck KGaA, Germany) before blood collection ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) containing protease and phosphatase inhibitors (cOmplete™ and PhosTOP™, Roche/Merck, Dorset, UK), 1 mM sodium orthovanadate and 1 mM phenylmethylsulfonyl fluoride and incubated for 30 min on ice.
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bioRxiv - Biochemistry 2024Quote: ... supplied with one tablet of ethylenediaminetetraacetic acid free SigmaFAST protease inhibitor cocktail tablet (Merck KGaA, Darmstadt, Germany) per 100 ml buffer ...
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bioRxiv - Physiology 2020Quote: ... Insulin concentration was determined using the MILLIPLEX® MAP Human Metabolic Hormone Magnetic Bead Panel (Merck KGaA, Darmstadt, Germany) according to the manufacturer s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Purified human integrin α5β1 and anti-α5 (MAB1956Z) and anti-α5β1 (MAB1999) antibodies were from Merck (Kenilworth, NJ, USA). Volociximab was from Novus Biologicals (Littleton ...
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bioRxiv - Cell Biology 2021Quote: The digoxigenin-labeled antisense probe targeting bases 32-500 of human GNRH1 mRNA (NM_001083111.2) was transcribed in the presence of digoxigenin-11-UTP (Merck Millipore) in a reaction mixture containing linearized cDNA template (1 µg) ...
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bioRxiv - Biochemistry 2020Quote: ... was equilibrated with increasing concentrations of NAb (30 pM–1.0 µM) in the presence of 90% heat-inactivated human serum (H5667, Merck) at a temperature of 4 °C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with unlabeled SARS-CoV-2 spike S1 at increasing concentrations (100 pM–3.2 µM) in the presence of 90% heat-inactivated human serum (H5667, Merck) and incubated at 4 °C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were cultured in TexMACS Medium (Miltenyi Biotech) supplemented with 5% heat-inactivated human AB serum (Merck), 100 units/mL penicillin (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... and primers anchored in the homology arms of the HDRT specific for KI at the human TRAC locus (Merck). PCR amplicons (typically 2.5 mL ...
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bioRxiv - Cell Biology 2023Quote: 293T human embryo kidney (HEK293T) cells were grown in Dulbecco’s modified Eagle’s medium (4.5 g/l glucose) (Merck, Dorset, UK) enriched with 10% fetal bovine serum (FBS) ...
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bioRxiv - Systems Biology 2021Quote: ... protease inhibitor EDTA-free (cOmplete™, 1 tablet per 50 mL, cat no. 11873580001, Merck KGaA, Darmstadt, Germany), 1 mM MgCl2 ...
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bioRxiv - Systems Biology 2020Quote: Wnt signal inhibitor (CK) stock (2.4–3 mM): CKI-7 dihydrochloride (#C0742-5MG, Merck & Co., Inc., NJ, USA) diluted in distilled water (Otsuka Pharmaceutical Factory ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EGTA and protease inhibitors) on an Amicon 30 kDa cut off concentrator (Merck, Cat# UFC 503096). Protein concentration was determined using a BCA protein assay (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... beads were incubated with astrocyte lysate buffer supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) for one hour at 4°C on a rotating wheel ...
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bioRxiv - Molecular Biology 2022Quote: AKT1/2 phosphorylation was inhibited with InSolution™ Akt Inhibitor VIII (1-10 µM, Merck KGaA, Darmstadt, Germany) in FCS-free medium for 3 hours prior to treatment with HGF (10 ng/µl).
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bioRxiv - Cancer Biology 2020Quote: Cells were washed twice with cold PBS and lysed by addition of Tris-buffered saline (TBS)-lysis buffer (TBS [50 mM Tris-HCl pH 7.5, 150 mM NaCl], 0.5% Nonidet P-40, 1× protease inhibitor cocktail [Merck] ...
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bioRxiv - Cell Biology 2019Quote: ... cells released from double thymidine arrest were incubated 4 hrs later with CDK1 inhibitor RO-3306 (Merck millipore) at 9µM concentration for 4 hrs ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HA9 (inhibitor of the substrate binding domain of Hsp70) and brefeldin A (disrupts Golgi apparatus, Sigma-Aldrich, Merck) were added for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in lysis buffer (50 mM Tris-Cl pH 8.0, 300 mM NaCl, cOmplete Protease Inhibitor Cocktail [Merck]) and lysed by sonication (19 mm probe ...
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bioRxiv - Microbiology 2022Quote: ... 150 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 1:100 diluted EDTA-free protease inhibitor cocktail [Merck]) for 45 min before clarification (21,000×g ...
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bioRxiv - Immunology 2022Quote: ... the pellet was resuspended in 150 μl of 8M urea buffer containing 1x protease inhibitor cocktail (Merck, Germany). It was performed 6 cycles of thermal shock ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mM imidazole and 1 mM TCEP) supplemented with protease inhibitor cocktail EDTA-free (Merck Millipore; 1:200). Cells were stored at −80°C until use ...
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bioRxiv - Immunology 2024Quote: ... anti-DNP IgE sensitized BMMCs were pre-incubated with inhibitors or blocking antibody: 50 µM Y-27632 (Merck), 50 µM blebbistatin (Merck) ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The total proteins were extracted by IP lysis buffer (Pierce) with a protease and phosphatase inhibitor cocktail (Merck), and then centrifuged at 12,000 g for 10 minutes at 4 °C to remove cell debris ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with protease inhibitor cocktail set III (Cat No. 539134-1 set, 1:100, Merck Millipore, Rahway, NJ). The lysate was centrifuged at 16,000 g at 4 LJ for 10 minutes to remove insoluble samples ...
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bioRxiv - Genomics 2023Quote: ... 1µM PD032552 inhibitors (both from Cell Guidance Systems) and 1000 U/mL units of Leukemia Inhibitory Factor (Merck). Cells were passaged with 0.05% accutase (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were lysed in lysis buffer (50 mM Tris HCl pH 7.4, 150 mM NaCl, 0.5% Triton X, 1 x cOmplete protease inhibitors [1x = 1 tablet in 40mL; Merck]), sonicated ...
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bioRxiv - Genomics 2023Quote: ... Cell nuclei were isolated using a cell lysis buffer (5mM Pipes pH8.0, 85mM KCl, 0.5% NP40, Complete protease inhibitor cocktail (Merck)) for 20min ...
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bioRxiv - Cell Biology 2024Quote: ... The cell pellet was resuspended in 200µL of lysis buffer (8M urea, 50mM Tris, pH 7.5, and protease inhibitors; Merck) and lysed by vortexing with glass beads (Scientific Industries ...
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bioRxiv - Cell Biology 2024Quote: Treatments for the LC3 assay included 3 hours with mTOR inhibitor Torin1 (Merck-Millipore, final concentration 250 nM), Bafilomycin A1 (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and then were scraped with RIPA lysis buffer containing phosphatase and protease inhibitor cocltails (catalog number 04906845001, Merck) and lysed for 30 min on ice ...
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bioRxiv - Biochemistry 2024Quote: ... 500 μM of the protease inhibitor 4-(2-aminoethyl)-benzolsulfonyfluorid hydrochloride (BioChemica) and 5 U/mL-benzonase (Merck) were added (final concentrations) ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs (2 × 106 cells) were plated onto 48-well plates (NalgeNunc) in RPMI-1640 with 5% inactivated male human AB serum (Merck) for 3 hours ...
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bioRxiv - Immunology 2021Quote: ... that serves as support for the culture system was prepared seeding human dermal fibroblasts (200,000) re-suspended in a fibrin gel made of fibrinogen (Merck-Millipore), thrombin (Merck-Millipore ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: Wild-type Xenopus tropicalis females and males were primed with 20U and 10U PREGNYL© human chorionic gonadotropine (hCG) (Merck), respectively ...
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bioRxiv - Developmental Biology 2022Quote: Ovulation was induced by injecting adult female Xenopus laevis with 600 units of human chorionic gonadotropin (HCG, MERCK Animal Health) and animals were kept at 16 dc overnight ...
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bioRxiv - Bioengineering 2021Quote: Isolation of fresh human PBMCs was initiated within 1 h after blood collection using Histopaque® 1077 (10771; Merck KGaA) and standard density centrifugation (800 rcf ...
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bioRxiv - Bioengineering 2021Quote: The rat INS-1 832/3 cell line (insulinoma cell line stably transfected with human insulin; hereinafter INS-1) was obtained from Merck. A HUVEC (human umbilical vein endothelial cell ...
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bioRxiv - Cancer Biology 2021Quote: ... and SPPL2A and control siRNA (IDT) were transfected into two human cell lines (HMV-2/CVCL_1282 -Merck-, WM3211/CVCL_6797 -Rockland-) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... At day 10 cells were passaged at a 2:3 ratio into 12 well cell culture plates coated with 15 µg/ml human plasma fibronectin (Merck) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Synthetic Biology 2022Quote: ... Aliquots of our working standard were generated by spiking 50 μL of our generated VLPs into 950 μL of human plasma (Merck). The calibration curve samples and the aliquots of working standard were then tested using a cobas® 6800 system (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... Another washing step was conducted as before and the Anti-Human IgE (ε-chain specific) detecting antibody (Merck, Darmstadt, Germany) was diluted 1:1000 (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... T cell medium (human serum was replaced with FCS during thawing) and incubated for 15 minutes with 25U/mL benzonase (Merck). After washing ...
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bioRxiv - Developmental Biology 2023Quote: Ovulation was induced by injecting adult female Xenopus laevis with 600 units of human chorionic gonadotropin (HCG, MERCK Animal Health) and animals were kept at 16 dc overnight ...
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bioRxiv - Biophysics 2023Quote: ... for 15 min at 20°C and the stamps were dried under a nitrogen flow A solution was then prepared by mixing 40 µL human fibronectin (Merck) and 960 µL of demineralized water ...