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Citations for Merck :
1051 - 1100 of 1360 citations for Recombinant Human CRYAB Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the target protein were concentrated to 5 mL with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was loaded onto a 320 mL of HiLoad Superdex 75 (GE Healthcare Life Science ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The total proteins were extracted by IP lysis buffer (Pierce) with a protease and phosphatase inhibitor cocktail (Merck), and then centrifuged at 12,000 g for 10 minutes at 4 °C to remove cell debris ...
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bioRxiv - Microbiology 2023Quote: ... The remaining RNA was combined with 25 μl of Magna ChIP Protein A+G magnetic beads (Merck Millipore) coated in 5μl of anti-m6A antibody (Merck Millipore ...
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bioRxiv - Synthetic Biology 2023Quote: ... Purified protein was concentrated with the use of a 3K Amicon® Ultra 0.5 mL Centrifugal filter (Merck), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Unbound proteins were removed by spin column purification (100kDa cut-off, Amicon® Ultra centrifugal filters (UFC210024, Merck)) ...
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bioRxiv - Immunology 2023Quote: The I-Ek-specific 14.4.4 mAb was purified from hybridoma supernatant using a protein A/G column (Merck) and gel filtration (Superdex-200 10/300 GL ...
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bioRxiv - Cell Biology 2023Quote: ... the purified protein was dialyzed against 20 mM HEPES using an Amicon ultra-15 (Merck, Rahway, NJ, USA).
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bioRxiv - Neuroscience 2023Quote: ... and then protein was loaded into Superdex 200 and concentrated using Amicon Ultra-4 (3K cut off, Merck). The labeling percentage was calculated according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The peak fractions containing the protein were pooled and concentrated in centrifugal concentrators (CLVG01230, Merck Millipore, Cork, Ireland) to a final concentration of 30 mg/ml for crystallization ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were precipitated with 50% (w/v) trichloroacetic acid (Roth) and washed once with ice-cold acetone (Merck). Protein pellets were resuspended in 1 x sodium dodecyl sulphate (SDS ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined and both fractions were incubated with Anti-FLAG® M2 Magnetic Beads (Merck, M8823). Beads were washed once then each sample was split for MS ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoblotting and detection were performed on the PVDF membrane with SNAP i.d.® 2.0 Protein Detection System (Merck) by using anti-myc (A00704 ...
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bioRxiv - Molecular Biology 2023Quote: ... This protein sample was concentrated 10 times using a 3.5 kDa Amicon Ultra 15 mL device (#UFC500324, Merck) at 3,000 x g 80 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... After the protein was collected the following day and concentrated using 10 kDa MWCO Amicon Ultra (Merck Millipore). The protein concentration was monitored periodically during the process using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: 500 μg of crude cell lysate was precleared with 10 μl of PureProteome Protein A Magnetic Beads (Merck Millipore ...
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bioRxiv - Biophysics 2024Quote: ... The fractions containing the target protein were concentrated to 5 ml with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was incubated with uTEV3 protease25(Addgene ...
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bioRxiv - Biochemistry 2024Quote: ... The cleaved protein was collected and concentrated using 100 kDa MW cut-off spin concentrators (Amicon Merck-Millipore) separated by size-exclusion chromatography (SEC) ...
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bioRxiv - Genetics 2024Quote: ... cell surface protein samples were concentrated into 0.1 ml volumes using a 30 kDa centrifugal concentrator (Merck Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... and then incubated with pre-equilibrated protein G agarose plus anti-FLAG M2 (F3165, Merck, 1 μl/IP), or 5 μl/IP anti-GFP (A-11122 ...
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bioRxiv - Microbiology 2020Quote: ... Collected cells were immediately spun down and resuspended in BugBuster® Protein Extraction reagent (Novagen, Merck and Co. Inc) to lyse the bacterial cells and release proteins ...
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bioRxiv - Genetics 2022Quote: ... 25 μg chromatin was diluted with IP dilution buffer and pre-cleared with Protein A agarose beads (Merck-Millipore) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on an SDS-PAGE gel and then transferred to a nitrocellulose (NC) membrane (Merck Millipore, ISEQ00010), which was incubated with individual protein-specific primary antibodies at 4 °C overnight on a shaker ...
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bioRxiv - Physiology 2020Quote: ... Proteins in the conditioned medium were concentrated up to 1.2ug/ul of total protein using cellulose membrane Ultra-4 Centrifugal Filter Unit of 3KDa cut-off (Merck) as per the manufacturer instructions.
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bioRxiv - Microbiology 2019Quote: ... For this purpose, proteins were blotted semidry (12 V, 20 min) on a PVDF membrane (PSQ membrane, 0.2 µm, Merck Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... Portions of 10 g cell pellet were resuspended in 50 mL Bugbuster (BugBuster® Protein Extraction Reagent, Merck Millipore) supplemented with 5 µL Benzonase Nuclease (Novagen ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: Proteins separated on PAGE gel were transferred to a PVDF membrane (Immobilon-P/Immobilon-FL, 0.45 μm, Merck Millipore) using Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized total proteins were separated by SDS-PAGE and electro-blotted onto a PVDF membrane (Merck Millipore; Billerica, MA). The membrane was first blocked with 5% skim milk in PBST (Phosphate Buffered Saline with Tween 20) ...
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bioRxiv - Neuroscience 2021Quote: ... Detection of newly synthesized proteins was carried out by an anti-puromycin antibody (clone 12D10, mouse-monoclonal, MABE343; Merck), an anti-HuD antibody (ab96474 ...
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bioRxiv - Cancer Biology 2020Quote: ... Thirty micrograms of cellular protein were resolved by 10 % SDS PAGE and were transferred to Immobilin P 0.45 μm PVDF membrane (Merck). Bound IgG was visualised with horseradish peroxidase-conjugated secondary antibodies and the Clarity™ Western ECL Substrate (Cat no ...
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bioRxiv - Cell Biology 2021Quote: ... Virotrap particles containing protein complexes were purified in a single step using biotinylated anti-FLAG BioM2 antibody (#F9291, Merck) and Dynabeads MyOne Streptavidin T1 Beads (#65601 ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing the capsid protein were pooled and concentrated using an Amicon Ultra-15 centrifugal filter (Merck Millipore), and the buffer was exchanged to a final storage buffer composed of 50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Microbiology 2022Quote: ... HRP-conjugated anti-goat or anti-mouse IgG (dilution 1:1,000) were added and protein complexes were visualized using o-phenylenediamine (Merck). The absorbance was then measured at 490 nm employing PowerWave HT (Bio-Tek Instruments ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was recovered and the protein concentration was estimated using the Direct Detect® Infrared Spectrophotometer (Merck-Millipore). 400 µg protein were incubated with 40 µL of streptavidin coupled DynabeadsTM M-280 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Molecular Biology 2020Quote: ... Total protein content in each eluate was measured using infra-red spectroscopy (Direct Detect, Merck Millipore, Burlington, MA, USA). The remainder was aliquoted and stored at −80 °C.
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bioRxiv - Biochemistry 2020Quote: ... Samples were subjected to SDS-PAGE and proteins were transferred to Polyvinylidene difluoride (PVDF) membranes (Immobilon®-P, Merck). The ATP2 proteins fused to the BAD tag were detected with the Avidin HRP-conjugate probe (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... the protein extracts (15 μg/sample) were blotted on a primed 0.45 μm polyvinylidene fluoride (PVDF) membrane (Merck-Millipore). Protein spots were stained using Direct Blue (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The sections were then incubated overnight in PBS with 0.2% BSA and 0.05% Tween-20 with primary antibodies directed against SARS-CoV-2 Nucleocapsid Protein (1:500; mouse monoclonal, # ZMS1075, Merck); Iba1 (1 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 4.2 μg PARG (in-house) per 10 mg protein and 10 μl of Benzonase (Merck Millipore, Darmstadt, Germany) per 30 ml were then added to each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing the proteins of interest were pooled and concentrated using Amicon Ultra-15 30K spin concentrators (Merck Millipore) before further purification using a HiLoad 16/600 Superdex 200 prep grade size exclusion column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was used to determine the total protein concentration by the Bradford method (Bradford reagent, Merck, Darmstadt, Germany), in a microplate reader at 595 nm ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Genomics 2022Quote: ... Total chromatin was pre-cleared for 1h at 4°C with rotation using protein A sepharose beads (P9424, Merck). 3 µg of anti-histone H3 (Abcam ...
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bioRxiv - Immunology 2020Quote: ... Collected proteins were buffer exchanged into PBS via multiple rounds of centrifugation in 10 kDA ultrafiltration tubes (Merck, USA). The purified protein content was measured by Bradford assay (B6916 ...
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bioRxiv - Immunology 2020Quote: ... 20 μg of brain lysate was examined by SDS-PAGE and proteins were blotted on PVDF membrane (Immobilon, Merck). Membrane was blocked with 2% skim milk and subsequently incubated with anti-DJ-1 antibody overnight ...
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bioRxiv - Immunology 2020Quote: ... and the fractions with the target protein were mixed and concentrated using Amicon Ultra-15 Centrifugal filters (Merck Millipore) with a 3 kDa membrane cutoff (Figure 10).
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bioRxiv - Developmental Biology 2021Quote: ... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...