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Citations for Merck :
1051 - 1098 of 1098 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated in a 10% (v/v) HS PBS solution supplemented with primary mouse anti-β-tubulin III antibody (1:100; Merck) overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were permeabilized with 100% ice-cold methanol for 20 min at -20°C and rehydrated by washing with PBS and then stained for viral hexon protein expression with an anti-hexon primary antibody (mouse; Merck; #MAB8052) and an anti-mouse secondary antibody coupled to AF488 or AF647 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed in PBS for 3 times and Alexa Fluor 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) was added for immunostaining in the dark for 2 hours at RT ...
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bioRxiv - Biochemistry 2023Quote: ... and mouse BRCA2 (amino acid residues: 2232-2283) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6-MBP tag and an N-terminal TEV-cleavable MBP tag ...
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bioRxiv - Microbiology 2023Quote: ... TNF-alpha and IL-10 in serum were determined using bead-based Multi-Plex kits (MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel 96-Well Plate Assay, Merck KGaA ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting immunocomplexes were detected by HRP-conjugated anti-mouse IgG or anti-rabbit IgG secondary antibodies and enhanced chemiluminescent (ECL) reagents (WBLUF0100, Merck Millipore).
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bioRxiv - Immunology 2024Quote: ... The cells were washed three times in PBS 1× and incubated for 1 hour at 37°C with 40 μL of mouse anti-DENV complex monoclonal antibody MAB8705 (Merck Millipore) diluted 1:200 in PBS 1× ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were exposed to the appropriate peroxidase-conjugated secondary antibodies (1:5000 goat anti-guineapig IgG A108P or 1:10000 sheep anti-mouse IgG A5906; Sigma-Aldrich, MERCK) for 1.5h and bands were visualized by chemiluminescence using the Clarity ECL Western blotting substrate (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... and 300 µg of protein was then incubated with 1 µg of a mouse anti-53BP1 antibody (1μg/ml, Merck millipore, MAB3802) O/N at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: The contents of CS in mouse serum and cells were measured with ELISA kits (Cat. No. MAK057; Sigma-Aldrich, Merck KGaA).
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bioRxiv - Cell Biology 2020Quote: ... GFP (1:2000) (Faix et al., 2001) or mouse monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000; #CB1001-500UG, Merck (Darmstadt, Germany)) and anti-fascin antibody 5E2 (undiluted hybridoma supernatant) ...
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bioRxiv - Physiology 2021Quote: ... in maternal serum and placenta was performed with MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assays (MCYTOMAG-70K, Merck Millipore, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: Quantification of Th1/Th2 cytokines in supernatant of splenocytes was performed using mouse high sensitivity T cell magnetic bead panel assay (MHSTCMAG-70K, Merck, Darmstadt, Germany). 5×105 isolated splenocytes were co-cultured with different stimuli in 200 μL RPMI + 10% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min each at RT and incubated with secondary antibodies in TPBS-TS-1% for 4h at 4°C (Alexa Fluor 546 anti-Mouse (Merck, A-11018), Alexa Fluor 488 anti-goat (Abcam ...
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bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunofluorescence staining of the DNA double-strand-break marker γH2AX [32] was performed using a JBW301 mouse monoclonal anti-γH2AX antibody (Merck, Darmstadt, Germany) and an Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit anti-Iba1 1:1000 (Wako Chemicals GmbH, Neuss) or double-stained with goat anti-td-Tomato and mouse anti-NeuN 1:500 (Merck Millipore, Darmstadt). Counter-staining for nuclei with 42,6-diamidin-2-phenylindol (DAPI ...
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bioRxiv - Cancer Biology 2021Quote: ... β-actin which was used as loading control was detected using a rabbit polyclonal IgG against mouse β-actin (A2103, Merck, Darmstadt, GER) diluted 1000-fold ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain) was cloned into pET-45b(+) vector (Novagen/Merck, Darmstadt, Germay) via ‘in vivo assembly’ (35 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were also probed with either a mouse monoclonal antibody against GAPDH or a mouse monoclonal antibody against beta-actin (dilutions of 1:10,000 and 1:1000; Sigma-Aldrich/Merck group, USA) and with the IRDye® 680CW secondary antibody from Eurobio Ingen (926-32220 ...
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.
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bioRxiv - Bioengineering 2024Quote: ... The scaffold was then rinsed three times with PBS and incubated with Alexa Fluor™ 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) and CF™ 633 conjugated secondary donkey anti-rat IgG antibody (1:100 ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was rinsed with TBS buffer and incubated with the primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) (1:2,500 ...
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bioRxiv - Microbiology 2024Quote: ... Infection foci were visualised using the appropriate secondary antibody coupled with horseradish peroxidase (HRP; 1:5000, Sheep Anti-Mouse IgG A5906, Sigma-Aldrich, Merck, Germany) and TrueBlueTM chemical (SeraCare ...
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bioRxiv - Microbiology 2024Quote: ... Actin was detected with monoclonal mouse antibody coupled with horseradish peroxidase (HRP; 1:75,000; A3854, Sigma-Aldrich, Merck, Saint-Quentin Fallavier, France). Membranes were exposed to the appropriate peroxidase-conjugated secondary antibodies (1:5000 goat anti-guineapig IgG A108P or 1:10000 sheep anti-mouse IgG A5906 ...
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bioRxiv - Neuroscience 2024Quote: ... ACTH was measured by sandwich immunoassay using the MilliPlex MAP Mouse Pituitary Magnetic Bead Panel Kit (Merck Millipore; product code MPTMAG-49K). Corticosterone was measured using the Corticosterone EIA Kit (ImmunoDiagnosticSystems ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies (as appropriate) were added for 1 hour at room temperature (goat anti-rabbit/ goat anti-mouse, IgG, HRP conjugated; Merck Millipore (1:5000)) before visualisation using enhanced chemiluminescence (1.25 mM luminol ...
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bioRxiv - Plant Biology 2021Quote: ... the sections were incubated in 1× PBS containing 1% (1:100 dilution) mouse monoclonal anti-Flag M2 primary antibody (Merck, cat. no. F1804) and 0.1% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibodies used to image centrioles in expanded RPE-1 cells were rabbit anti-polyglutamate chain (polyE), pAb (IN105, 1:250) and mouse monoclonal anti-acetylated tubulin (Merck, T7451, 1:500). Secondary antibodies were goat anti-Rabbit Alexa488 (Thermo-Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... unbound primary antibodies were removed by washing in TBST and the blots were incubated with HRP-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG and IgM HRP conjugated antibodies (1:4000, Merck EMD Millipore, Ap130P), or goat anti-rabbit IgG antibody ...
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bioRxiv - Neuroscience 2022Quote: ... The animals used in our experiments were genotyped for expression of the transgene by quantitative PCR (qPCR) using the KAPA Mouse genotyping kit (Merck, Cat# MGKITKB-KK7301), with primer sequence (5’-3’ ...
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bioRxiv - Microbiology 2023Quote: ... the cells were stained with goat anti-rabbit Alexa Fluor® 555 (Thermo, 1:1,000) and mouse anti-IE Alexa Fluor® 488 (Merck, 1: 1,000) for 1 hour at RT ...
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bioRxiv - Microbiology 2023Quote: ... SPN infected A549s were fixed with chilled methanol followed by further processing with Duolink® In Situ Orange Starter Kit Mouse/Rabbit (MERCK, DUO92102-IKT) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then treated with antibodies against FLAG (Monoclonal ANTI-FLAG® M2 antibody produced in mouse; Merck CAT# F3165; 1:200 dilution) or CSNK1D (Anti-Casein Kinase 1 delta/CSNK1D antibody [AF12G4] ...
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bioRxiv - Genomics 2024Quote: ... with the following primary antibodies: goat anti-IL1RN (RD Systems Cat # AF280NA) in 1:1000 in blocking solution and mouse anti-β actin (MERCK Cat # A1978) and rabbit anti-vinculin (Cell Signaling Cat #18799 ...
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bioRxiv - Molecular Biology 2024Quote: ... The plate was washed three times with TBS-C blocking solution and incubated with goat-anti mouse IgG-HRP (A4416, Merck; dilution 1/10,000) added in TBS-C blocking solution and incubated during 1 h at RT ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then incubated for 1 h at room temperature with the monoclonal mouse Anti-Tom20 (1:500, MABT166, Merck & Co., Kenilworth, NJ, USA). The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500 ...
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bioRxiv - Systems Biology 2024Quote: Cells were analysed for protein expression by SDS polyacrylamide gel electrophoresis and Western blot analysis using the following antibodies: mouse monoclonal anti α-tubulin (#05-829, Merck Millipore, Burlington, US), rabbit α-p-AKTSer473 (#9271) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated for 1 hour with HRP-conjugated secondary antibodies (1:5000, anti-mouse, Sigma-Aldrich Catalogue No A9044; Flag, or 1:10000, anti-rabbit, Merck, Catalogue No GENA934; LpoA) in TBS-TM ...
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bioRxiv - Microbiology 2024Quote: ... were coated the day before with rabbit anti-mouse IgM antibody (100 μL/well, 1:500 dilution, SAB3701197, Sigma-Aldrich, Merck, Saint-Quentin Fallavier, France). Then ...
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bioRxiv - Microbiology 2023Quote: ... and untagged 1H6 (mouse monoclonal IgG2bκ) in PBS with 0.05 % sodium azide and 50 % glycerol were purchased from Merck (stored at -20°C with light protection). We used sterile-filtered 3 % bovine serum albumin (BSA heat shock fraction ...