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Citations for Merck :
1051 - 1100 of 1591 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: The concentration of protein extracts was determined by BCA assay kit with BSA standards (Merck).
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bioRxiv - Microbiology 2023Quote: ... The proteins were separated via 15% SDS-PAGE and transferred to nitrocellulose membranes (Merck-Millipore). Western blot analyses were then conducted using PilA- ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein bands were visualised using a chemiluminescence substrate (Immobilon Forte, Merck Life Science UK Ltd.) on the ChemiDoc Imaging system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... eluted proteins were then concentrated using amicon ultra-15 centrifugal filters (Cat# UFC901024, Merck Millipore).
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bioRxiv - Microbiology 2023Quote: ... and pellets were diluted in 1× BugBuster® (10× Protein Extraction Reagent, Novagen® Merck), 50 mM NaH2PO4 pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... and protein complex was eluted in lysis buffer 2 containing 2.5 mM D-desthiobiotin (Merck). Protein tags were removed by overnight cleavage with HRV 3C protease ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was transferred to an Immobilon®-FL PVDF membrane (Merck Millipore, Billerica, MA, USA) and blocked in Licor blocking buffer for 120 minutes (Licor ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein was extracted using RIPA buffer mixed with protease inhibitor cocktail (Millipore Merck, 539134) and quantified using Qubit® Protein Assay Kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... In brief supernatant protein concentration was standardised prior to being concentrated using Microcon® (Merck) 10Kda filter columns and centrifugation (14,000g 20 minutes) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were resolved via SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane (Merck, GE10600023) with a wet electroblotting system (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were concentrated using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore). Protein and ligand samples were degassed prior to each ITC experiment ...
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bioRxiv - Cell Biology 2024Quote: ... The chromatin (100 μg) was precleared with protein A agarose/Salmon sperm DNA beads (Merck) and 2 % taken as input ...
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bioRxiv - Biochemistry 2024Quote: ... Glycerol-free eluted protein was concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (Merck) at 10,000 x g ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was filtered through an Ultrafree-MC centrifugal device with hydrophilic PTFE membrane (Merck) at 10,000 x g ...
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bioRxiv - Plant Biology 2022Quote: ... 100 μg of protein were loaded into FASP filters (Amicon Ultra 30K, 0.5 ml, Merck) and tryptically digested over night following the FASP (Filter-Aided Sample Preparation ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 mins at 60°C with 10 mM dithiothreitol (Sigma, Merck) in 50 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Genetics 2023Quote: ... Anti-S6 Ribosomal Protein Antibody (used with 1:1000 dilution) was purchased from Merck (#ZRB1172). α-tubulin antibody (used with 1:5000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 75 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Immunology 2023Quote: ... EVA71 VP0/VP2 reactive proteins were detected using anti-EVA71 VP2 antibody clone 979 (Merck) at 1:2000 dilution and anti-mouse IgG HRP conjugate ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to PolyVinyliDene Fluoride 0.45 µm membranes (Immobilon-Fl, Merck Millipore, Darmstadt, Germany). Membranes were saturated 1 hour with a Tris-Buffered Saline blocking solution (TBS blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to FL-PVDF membranes (cat. No. IPFL00010; Merck Millipore; Massachusetts, USA). The membranes were incubated with ms anti-Y1-R (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously123 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated using SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-FL, Merck). The membranes were blocked for 30 min with Blocking-One or Blocking-One P (Nacalai Tesque ...
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bioRxiv - Physiology 2023Quote: ... The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously107 ...
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bioRxiv - Immunology 2023Quote: ... Protein bands were visualized by treating the membrane with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: Protein extracts were transferred into an Amicon Ultra Centrifugal filter device (10 kDa MWCO, Merck) for buffer exchange [50 mM Tris-HCL pH 7.5 ...
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bioRxiv - Physiology 2023Quote: The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously23 ...
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bioRxiv - Pathology 2024Quote: ... Proteins were then transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Biophysics 2024Quote: ... The dissolved protein solution was then repeatedly buffer exchanged with a centrifugal filter (Amicon, Merck) against the same buffer to eliminate the residual TFA ...
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bioRxiv - Microbiology 2024Quote: ... the separated proteins were semi-dry blotted onto an Immobilon-FL PVDF-Membrane (Merck, #IPFL00010) at a constant voltage of 30 V for 30 min ...
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bioRxiv - Genetics 2024Quote: ... Proteins were then separated using 10% SDS-PAGE and transferred to PVDF membranes (Merck Millipore). PVDF membranes were then blocked by 5% BSA ...
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bioRxiv - Microbiology 2024Quote: ... The proteins in the gel were transferred onto polyvinylidene difluoride membranes (Merck Millipore, Billerica, MA). After protein transfer ...
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bioRxiv - Biochemistry 2024Quote: ... Each of the purified proteins was concentrated by an Amicon Ultra centrifugal filter units (Merck) with an appropriate molecular weight cutoff followed by filtration through a 0.45 μm pore size membrane filter (Merck) ...
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bioRxiv - Pathology 2024Quote: ... Separated proteins were transferred onto polyvinylidene difluoride membranes (Immobilon-P; Merck Millipore, Burlington, MA, USA). The membranes were reacted with the following primary antibodies ...
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bioRxiv - Genetics 2024Quote: ... Proteins separated by 10% SDS-PAGE were transferred onto a PVDF membrane (Immobilon-E, Merck) and blocked in 5% milk in Tris-Buffer Saline (TBS ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates were separated by SDS-PAGE gels and transferred to PVDF membranes (Merck, IPVH00010). Membranes were stained with Ponceau-S ...
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bioRxiv - Genetics 2024Quote: ... and the protein concentration was determined using a Direct Detect® Infrared Spectrometer (Millipore, Merck).
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bioRxiv - Microbiology 2024Quote: ... and the proteins were then concentrated to 125 µM using Amicon Ultra 3K (Merck Millipore). The oligomers were obtained by sequential dialysis adapting the protocol (https://www.nature.com/articles/nature09640) ...
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bioRxiv - Neuroscience 2024Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were desalted with the use of Amicon 50 kDa cut-off centricons (Merck) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Boiled protein samples were separated by SDS-PAGE and electroblotted onto an Immobilon-P (Merck) membrane ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were separated by 12% SDS-PAGE and detected using anti-GFP (1:2000, Merck) and anti-Myc (1:2000 ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted proteins were buffer-exchanged using PD-10 desalting columns Disposable (Sigma Aldrich; Merck) against storage buffer (50 mM HEPES ...
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bioRxiv - Synthetic Biology 2024Quote: ... The eluted proteins were desalted using a Hitrap Desalting Column (Cat#GE17-1408-01, Merck) and eluted in binding buffer (pH 7.4 ...
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bioRxiv - Bioengineering 2024Quote: ... The proteins were concentrated using Amicon Ultra Centrifugal Filters-0.5 10 kDa MWCO (Merck Millipore) and frozen at a stock concentration of 8 mg/mL.
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bioRxiv - Developmental Biology 2021Quote: ... males were boosted with 100 units (U) of human chorionic gonadotropin (HCG: Chorulon®, Merck, NADA NO.140-927, Code No. 133754) and females were primed with 10U HCG ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rat slices were exposed to 3 μM dihydroethidium (DHE) while in humans’ sections it was added 4 μM DHE (Merck, Darmstadt, Germany) for 30 minutes at 37ºC 51 ...
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bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...