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Citations for Merck :
1051 - 1100 of 2196 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
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bioRxiv - Immunology 2022Quote: ... MCP-3 and RANTES were quantified using a multiplex kit (MILLIPLEX MAP; Merck, Burlington ...
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bioRxiv - Immunology 2021Quote: ... Peritoneal macrophages were isolated from mice 3 days after injection of thioglycollate (MERCK) and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: ... RNAs were concentrated by ultrafiltration using Amicon Ultra 3 MWCO centrifugal filters (Merck) and stored at −80 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... washed again 3 times 15 min and developed using Millipore Crescendo ECL (Merck). For western blot quantification ...
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bioRxiv - Pathology 2020Quote: ... Non-specific binding sites were blocked via incubation in 3% H2O2 (Merck, Germany) and BSA respectively ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were mounted using a 3:1 solution of Canada balsam (Merck, # 1016910100) and Histoclear (HS-202 HISTO-CLEAR II ...
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bioRxiv - Cell Biology 2020Quote: ... or rabbit anti-p34-Arc/ARPC2 (Arp2/3, 1:100, Merck, 07-227). This was followed by incubation with appropriate Alexa Fluor-conjugated secondary antibodies (1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... washed 3 times in PBS with 1x cOmplete Protease Inhibitor Cocktail (Merck, 11697498001).
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore) at 3500 rpm and 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT) and concentrated using a 3 kD MWCO centricon (Merck Millipore). For best performance in the iSAT reaction ...
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bioRxiv - Systems Biology 2022Quote: ... 10+3) were seeded onto fibronectin coated plates (20 µg/mL, FC010 Merck) at a density of 0.25 x 106 cells/cm2 and cultured for three (d3 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... namely using 1 g/L MS-222 (Ethyl 3-aminobenzoate methanesulfonate, Merck #E10521) and subsequent exsanguination by cutting the gill arches ...
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bioRxiv - Developmental Biology 2024Quote: ... The clean coverslips were sequentially incubated in 0.5% (3-aminopropyl)triethoxysilane (APTS) (Merck) for 3min ...
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bioRxiv - Neuroscience 2023Quote: ... The eluate was concentrated with Amicon Ultra-15 (Merck Millipore, MWCO 3 K) to a protein concentration of 60 µM.
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bioRxiv - Neuroscience 2023Quote: ... anti-cathepsin B (Abcam, ab92955, 1:1,000 and Merck, Ab-3 1:100) and anti-β-actin mouse monoclonal antibody (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... and the pH was adjusted to 5.5 with KOH (1310-58-3, Merck). The plates containing the seeds were stratified for 2 days at 4°C in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... containing 0.17mg/ml tricaine (ethyl-3-aminobenzoatemethanesulfonate, ref. n°E10521, Merck/Sigma-Aldrich). WM983B cells of different lysosome clustering status (spread ...
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bioRxiv - Biochemistry 2023Quote: ... followed by an Amicon ®Ultra centrifugation filter (3 kDa cut-off, Merck). The sample was transferred to a 5 mm NMR-tube (Labscape™ Essence ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples was concentrated by 3 kDa Amicon Ultra centrifugal filters (Merck Millipore, R0NB30416) with 100 mM NH4HCO3 to exchange the buffer and retain globular particles above 3 kDa ...
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bioRxiv - Immunology 2023Quote: ... Tissue slides were blocked in 1X PBS with a 3% BSA (Merck, A7906), 10% Donkey serum (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) was acquired from Merck (Darmstadt, Germany). LNP were produced using microfluidic mixing with the Dolomite Microfluidics system (Dolomite Microfluidics ...
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Highly amine-reactive graphene-oxide EM grids for biochemical surface modification in aqueous bufferbioRxiv - Molecular Biology 2023Quote: ... 4-Amino-1-butanol (#178330) and 3- glycidoxypropyltrimethoxysilane (#440167) were obtained from Merck. Gold nanoparticle- PEG-amine conjugate was custom-synthesized by NanoPartz (Loveland CO ...
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bioRxiv - Immunology 2024Quote: ... The slides were blocked overnight in PBS solution containing 3% BSA (Merck, #A7906), 10% donkey serum (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: mouse monoclonal anti-SARM1 (Chen et al., 2011) (1:5000) and mouse anti-β-actin (Merck, A5316, 1:2000) as a loading control ...
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bioRxiv - Developmental Biology 2024Quote: ... For each IVF session one epididymis from 12-week-old WT and one from Trim66-null were transferred into 90 μL of capacitation medium, consisting of TYH (Takeo & Nakagata, 2011) with 0.75 mM methyl-β-cyclodextrin (MBCD, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany). Spermatozoa were allowed to disperse from the tissue and incubated for 30 min in a 5% CO2 incubator at 37°C.
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bioRxiv - Neuroscience 2019Quote: Immunohistochemistry was performed on colon sections as described earlier on brain sections58 using additional antibodies (α-Syn: ab27766, Abcam or L001, Enzo; NeuN: MAB377, Merck; MAP2: ab70218 Abeam; TH: 657012, Merck; GFAP: Z0334, Dako).
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bioRxiv - Immunology 2021Quote: ... When cells were treated with IFN-α prior to infection (pre-treated) this was done with 1000 IU/ml IFN-α2b (referred to as IFN-α from here on; IntronA, Merck Sharp & Dohme Ltd). IFN-α remained on cells for the duration of experiments ...
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bioRxiv - Immunology 2020Quote: ... When cells were treated with IFN-α prior to infection (pre-treated) this was done with 1000 IU/ml IFN-α2b (referred to as IFN-α from here on; IntronA, Merck Sharp & Dohme Ltd.) 18 h prior to infection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... λem□=D525□nm) and l-α-phosphatidylethanolamine-N-(lissamine rhodamine-B sulfonyl) (ammonium salt) (PE-Rh-B) (fluorescent acceptor, λem□=□595░nm) (Merck KGaA, Darmstadt, Germany) in 1:7 DM ratio ...
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bioRxiv - Systems Biology 2024Quote: Cells were analysed for protein expression by SDS polyacrylamide gel electrophoresis and Western blot analysis using the following antibodies: mouse monoclonal anti α-tubulin (#05-829, Merck Millipore, Burlington, US), rabbit α-p-AKTSer473 (#9271) ...
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bioRxiv - Microbiology 2019Quote: C6/36 or Huh7 were seeded in 8-well chambers and confluent monolayers were incubated with 150 or 300 μg/mL of HDL (Merck, USA) in specific growth medium without FBS for 1.5 h ...
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bioRxiv - Developmental Biology 2022Quote: ... Organoid cells were cultured in self-renewing medium with ROCKi for 8 days before Dox (Doxycycline hyclate, 2 µg/ml, Merck, D9891) and TMP (Trimethoprim ...
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bioRxiv - Neuroscience 2020Quote: ... was transfected into COS-7 cells cultured in a well of 8 well chamber slide glass using GeneJuice Transfection Reagent (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were loaded on 8% gels and separated by SDS-PAGE followed by the transfer done on to Immobilon-P® (Merck) PVDF membranes at 106 V for 75 minutes.
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bioRxiv - Microbiology 2021Quote: ... NoDice/ΔPKR cells were seeded at the density of 8.104 cells per well in 8-well Millicell® EZ Slides (Merck Millipore), transfected and infected as described previously ...
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bioRxiv - Physiology 2021Quote: ... Resulting lipid films were dissolved in 1 mL of n-hexane containing a C19:0 as internal standard (1.03 μg mL−1, CAS number 1731-94-8, Merck, Darmstadt, Germany) with addition of 200 μL of a solution of potassium hydroxide (KOH ...
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bioRxiv - Genetics 2020Quote: ... of total protein suspended in loading buffer were separated by SDS-PAGE using 8% or 4-20% gradient gels and transferred onto PVDF membranes (Immobilon-P or Immobilon PSQ, Merck Millipore) for immunostaining ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μg of protein per sample was loaded and separated on 8-12% SDS–PAGE gels and electro blotted onto polyvinylidenedifluoride (PVDF) membranes (Immobilon-P PVDF membrane 0.45μm; Merck-Millipore, Burlington, USA). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: Proteins (30 μg) were separated on an 8% SDS-PAGE gel and blotted onto a PVDF membrane (GE10600029 Merck, Darmstadt, Germany). Membranes were blocked for 5 minutes with EveryBlot Blocking Buffer (12010020 Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were resuspended in 8 M urea solution in 100 mM Tris-HCl and deposited on top of Microcon®-30K devices (Merck). Samples were centrifuged at 9400 × g ...
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bioRxiv - Bioengineering 2023Quote: ... and 70 samples were buffer-exchanged into 150 mM aqueous ammonium acetate solution (pH 8) using Amicon 50 kDa MWCO centrifugal filters (Merck Millipore). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... CTCF antibody (07-729, Merck Millipore; 5 μL per ChIP) was coupled with Protein G beads (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... Chromatin was incubated with 5 μL anti-CTCF (Merck-Millipore), 5 μg anti-H3K27ac (Abcam) ...
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bioRxiv - Genetics 2022Quote: ... or 5 μg rabbit IgG isotype control antibody (Merck-Millipore) for 16 h at 4°C with rotation ...
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bioRxiv - Cell Biology 2019Quote: ... dropped onto glass slides and stained with 5% Giemsa (Merck). Cohesion defects were counted in 25 metaphases per slide on two coded slides per condition.
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bioRxiv - Immunology 2019Quote: ... the tissue was mounted using 5% N-propyl gallate (Merck) in glycerol and imaged using a Zeiss Axio Scope A1 (Zeiss) ...
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bioRxiv - Neuroscience 2021Quote: ... and hydrated for 5 min in 99 % ethanol (100983, Merck), 5 min in 95 % ethanol ...
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bioRxiv - Microbiology 2021Quote: ... Parasite nuclei were stained using 5 µg/ml Hoechst (Merck) and Vectashield (Vector Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... and 10 µM fluorescein (46955, CAS 2321-07-5, Merck). The conductivity of 0% NaCl and 100% NaCl was respectively 5.99 µS/cm and 11.02 mS/cm and increased linearly with the NaCl concentration ...