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Citations for Merck :
1001 - 1050 of 2128 citations for Tetratricopeptide repeat protein 14 TTC14 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The supernatant (i.e., crude protein) was filtered through a 0.45 μm pore size membrane filter (Merck), applied onto HisTrap FF crude column (Cytiva ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell lysate was prepared and protein concentration was measured by Direct Detect® spectrometer (Merck). An equal amount of proteins (50 µg ...
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bioRxiv - Biochemistry 2024Quote: ... and the proteins were subsequently transferred to a PVDF membrane (#IPVH00010, Merck Millipore, Burlington, MA, USA). The membranes were washed with TBS-T (20 mM Tris (pH 7.5) ...
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bioRxiv - Immunology 2024Quote: ... HLA-G2 proteins in PBS were concentrated by Amicon Ultra-15 (NMWL 10,000 Da) (Merck Millipore), and the flowthrough solution from protein concentration was used as a negative control (PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein mixtures were incubated with 40μl PBST washed Streptavidin agarose resin (50% slurry, 69203-3 Merck) and incubated for 2h at room temperature on a roller ...
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bioRxiv - Biochemistry 2024Quote: Optional concentration of protein samples using Amicon® Ultra centrifugal filters (Merck Millipore Ltd., Carrigtwohill, IRL) was performed prior to buffer exchange to reduce sample volume ...
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bioRxiv - Microbiology 2024Quote: The gene encoding the arm segment protein was cloned into the pET-28b(+) vector (Novagen/Merck), providing a His6 tag at the C-terminus of the protein ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant proteins were concentrated and imidazole was diluted by using Vivaspin 20 (50 kDa, Merck) for FPLC-purified proteins or Amicon Ultra 0.5 mL Centrifugal Filters (50 kDa ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% Triton X100 supplemented with 1 mg/ml lysozyme and 1x cOmpleteTM protein inhibitors cocktail (Merck). Cdk2 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred on a PolyVinyliDene Fluoride (PVDF) membrane (Immobilon-P, Merck Millipore, Burlington, USA) using liquid transfer with 20 % methanol at 100V ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were concentrated at 3,500 rpm and 4 °C using Amicon Ultra centrifugal filter units (Merck) or using a stirred cell ultrafiltration device ...
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bioRxiv - Immunology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Merck-Millipore, Billerics, MA, USA) using a semi-dry transfer system (Trans Blot Transfer Medium ...
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bioRxiv - Plant Biology 2024Quote: ... and then the protein eluted with elution buffer (HEGX buffer with 100 mM DTT [Merck # 1.24511.0005]). The protein prep was then dialyzed with SnakeSkin Dialysis Tubing (3500 MWCO ...
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bioRxiv - Biochemistry 2024Quote: ... The protein mixture was concentrated using Amicon Ultra 3 kDa MW cut-off device (Merck Millipore) to ∼15 mg/ml and used to set up commercial crystallisation screens using the sitting drop vapour diffusion method at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Proteins contained in BALF and TS were concentrated 5x using an Amicon Ultra column 100kDa (Merck Millipore) and 20 min centrifugation at 4000xg and samples were stored at −80°C ...
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bioRxiv - Biophysics 2020Quote: ... the protein was concentrated by centrifugation using Amicon Ultra 15-30k centrifugal filters (Merck, Kenilworth, NJ, USA) at 3452 x g ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were run in SDS-PAGE and transferred to Immobilon Polyscreen PVDF transfer membranes (#IPVH00010, Merck Millipore) or Amersham Protan nitrocellulose membranes (#10600001 GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... Target protein expression was detected using Immobilon™ Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein lysates were resolved by SDS/PAGE under reducing condition and transferred to PVDF membranes (Merck Millipore). After blocking ...
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bioRxiv - Molecular Biology 2021Quote: ... Immuno-reactive proteins were visualized using the Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA). The detected bands on the scanned blots were quantitated with ImageJ freeware.
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bioRxiv - Bioengineering 2021Quote: ... the purified protein containing fraction was concentrated (Centriprep® Centrifugal Filters Ultacel® YM - 50, Merck Millipore).
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bioRxiv - Pathology 2021Quote: ... The proteins were cleaned using Amicon Ultra-4 Centrifugal Filter Devices (15 ml, 10 kD; Merck Millipore) to remove the elution buffer ...
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bioRxiv - Microbiology 2021Quote: ... Two microlitres of the supernatant was used for protein quantification using the Direct Detect system (Merck Millipore). The acquired concentrations of intracellular c-di-GMP were normalized to the total protein content.
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bioRxiv - Microbiology 2021Quote: ... Protein samples were separated by SDS–PAGE (4-20%) and blotted onto polyvinylidene fluoride membranes (Merck Millipore). Membranes were blocked for 1 h with Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2020Quote: ... and proteins were eluted using 100 µl of 150 ng/µl 3× FLAG Peptide (F4799, Merck KGaA) or HA peptide (HY-P0239 ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were transferred on an Immobilon™-P Membran (PVDF, 0.45 µm, Art. No. IPVH00010, Merck Millipore) for 16 h at 15 V using a Mini Trans-Blot® Cell (Art ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved by SDS-PAGE and transferred onto a PVDF membrane (pore size 0,2 μm, Merck, cat ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded protein was concentrated with Amicon Ultra-15 Centrifugal Filter Units (100 kDa NMWL, Merck Millipore) and remaining urea removed with a PD10 desalting column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: Denatured proteins were separated by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore IPFL00010). Membranes were incubated in a casein-based blocking buffer (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl of the supernatant were used for protein quantification using the Direct Detect system (Merck Millipore).
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bioRxiv - Microbiology 2021Quote: ... GGG-azide labelled proteins were concentrated to 25 μM on a 30 kDa Amicon Tube (Merck Millipore) in Tris/NaCl buffer and next labelled with 100 μM DBCO-Cy5 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The protein samples were separated by SDS-PAGE and transferred to PVDF membranes (Merck Millipore, Burlington, MA) by electroblotting ...
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bioRxiv - Microbiology 2020Quote: ... Lentivirus stocks were concentrated 25-fold using centrifugal protein concentrators (Amicon Ultra, 10-kDa cutoff; Merck, Poland) and stored at - 80°C.
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were separated from each sample by centrifugation through Amicon ® Ultra Centrifugal Filters (Merck, Millipore Ltd.) at 21.000 x g for 10 min at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing the proteins were concentrated with Amicon® Ultra-15 Centrifugal Filters Ultracel®-100K (Merck-Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... The separated proteins were then transferred to the PVDF membranes (0.45 µm Immobilon®-E; Merck Millipore). Membranes were blocked either with 2.5% nonfat milk in TBST (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... After eluting and concentrating the cleaved protein in Amicon Ultra-15 Centrifugal Filters 50K MWCO (Merck Millipore), size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2020Quote: ... Proteins in each lysate were resolved by SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Merck). The blots were incubated with the following primary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated by SDS-PAGE system and transferred to Immobilon-P membrane (Merck KGaA, Darmstadt, Germany). Immunoreactions were detected according to standard methods described previously [Yoshitake et al ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... proteins were separated with SDS-PAGE gels and transferred to PVDF membranes (Immobilon-P, Merck Millipore, USA). Blotted membranes were reacted with the specific primary antibodies shown in Supplementary Table 1 followed by peroxidase-linked secondary antibodies ...
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bioRxiv - Biochemistry 2022Quote: Protein extracts were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore-Merck, Germany). Membranes were blocked with 5% non-fat milk and incubated with a specific anti-biotin antibody overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the purified protein were concentrated using a 0.5 ml Amicon Ultra centrifugal filter (Merck Millipore) with a membrane molecular weight cut off 10 kDa.
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bioRxiv - Genetics 2022Quote: Whole-cell protein lysates were extracted in modified radioimmunoprecipitation assay buffer supplemented with protease inhibitors (#5892970001, Merck) and phosphatase inhibitor cocktail (#4906837001 ...
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bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... to 20 μg of mitochondrial protein and run with Tris-MOPS running buffer (#PCG3003; Sigma-Aldrich/Merck) on a 4-20% precast Trupage gel (#PCG2012-10EA ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were concentrated where necessary using Amicon filter devices with appropriate molecular weight cut-off (Millipore; Merck).
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd., Ireland). 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter ...