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Citations for Merck :
1001 - 1050 of 1633 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... + 10% heat-inactivated Fetal Bovine Serum (Eurobio) + 2 mM L-Glutamine (Merck) + 1% Penicillin/Streptomycin ...
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bioRxiv - Molecular Biology 2024Quote: ... and carefully layered over 2 mL of 20% sucrose (Merck Millipore, USA) in polypropylene tubes (Beckman Coulter ...
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... H4S1 phosphorylation was detected using the anti-phospho histone H4/H2A S1p antibody at 1:1000 (Upstate/Merck-Millipore); sf-GFP-Sps1 was detected using JL-8 anti-GFP antibodies (Takara/Clontech ...
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bioRxiv - Physiology 2022Quote: ... monoclonal anti-α-actinin (Sarcomeric) antibody produced in mouse (1:1000, Sigma-Aldrich, Merck, clone EA-53, Cat# A7811), at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... we filtered three aliquots of 1000 ml per water sample through 0.45μm 47mm white gridded mixed ester cellulose membranes (Merck, USA). Membranes were placed on 55 mm Baird-Parker with potassium tellurite enrichment agar plates and incubated at 37°C for 48 hours ...
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bioRxiv - Genetics 2022Quote: ... followed by overnight incubation at 4°C with primary antibodies (Anti-ASL, Abcam ab97370, 1:1000; Anti-GAPDH mouse, Abcam ab8245, 1:10,000; Anti-nitrotyrosine, Merck 05-233 ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining of puromycin was performed by incubation with the anti-puro monoclonal antibody (1:1000, Clone MABE343, Merck) for 60 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining of puromycin was performed by incubation with the anti-puro monoclonal antibody (1:1000, Clone MABE343, Merck) for 60 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were run on SDS/PAGE and analyzed for the expression of Phospho-Tyrosine and IDO1 using an anti-pTyr -1000 and anti-IDO1 (clone 10.1, Merck) antibodies ...
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bioRxiv - Genetics 2023Quote: ... The screens were performed without feeder cells in M15 medium supplemented with 1000 U/ml leukemia inhibitory factor (Merck). Cells were plated on flasks coated with 0.1% gelatin solution (Merck) ...
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bioRxiv - Immunology 2023Quote: ... benthamiana was validated by immunoblot analysis using a polyclonal anti-luciferase primary antibody (Merck; used in 1:1000 dilution).
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bioRxiv - Cancer Biology 2024Quote: ... followed by primary antibodies against γH2AX (the same antibody used for wester blotting) and 53BP1 (1:1000, MAB3802 Merck). Cells were washed and incubated with secondary anti-rabbit-Alexa Fluor 488 (1:500 ...
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bioRxiv - Physiology 2024Quote: ... Membrane was incubated overnight at 4°C with specific mouse monoclonal primary antibody anti-puromycin (Merck, MABE343, 1:1000) and after wash ...
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bioRxiv - Neuroscience 2021Quote: ... was diluted (1:1000) in 500 μl 0.1% PBST with 0.2% normal donkey serum (S30-100ML, Merck Millipore, Hertfordshire, UK) and sections were protected from light and incubated at RT with agitation for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at room temperature in darkness with the primary antibody solution containing mouse anti-vesicular Glutamate Transporter 1 (vGLUT1, 1:1000, MAB5502, Merck) or rabbit anti-Glutamate Decarboxylase 65-67 (GAD 65-67 ...
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bioRxiv - Neuroscience 2021Quote: ... After washing the sections were incubated overnight at 4°C with anti-NeuN antibody (1:1000, Merck Millipore, Cat. # ABN90P) in 1% normal goat serum and 0.1% Triton-X-100 in TBS ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections containing SN and VTA were incubated with antibodies directed against tyrosine hydroxylase (mouse monoclonal, 1:1000, Merck-Millipore MAB318) and mCherry (rabbit polyclonal ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at room temperature in darkness with the primary antibody solution containing mouse anti-vesicular Glutamate Transporter 1 (vGlut1, 1:1000, MAB5502, Merck) and rabbit anti-Glutamate Decarboxylase 65-67 (GAD 65-67 ...
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bioRxiv - Bioengineering 2020Quote: ... or mouse monoclonal β-actin antibody (1:1000 dilution; catalog no. catalog no. MAB 1501; Merck Millipore, Burlington, MA, USA) at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... slides were blocked for 30 min in 0.3 % Triton / 10 % normal goat serum / TBS followed by overnight primary antibody incubation (1:1000; rb TH; Merck Millipore) at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed and incubated overnight at 4°C with anti-Digoxigenin-AP antibody (45-11093274910 Merck-SIGMA, 1:1000). Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a rabbit anti-Kv4.3 primary antibody (1:10000, Alomone Labs) together with chicken (G)/mouse (L) anti-TH primary antibody (1:1000, Abcam / Merck) in a carrier solution (1% NGS ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... the slices were washed three times with a blocking buffer containing 1% bovine serum albumin and 0.25% Triton-X in phosphate buffer saline (PBS) and then incubated with primary antibodies (anti-tyrosine hydroxylase, rabbit polyclonal, 1:1000, Merck Millipore ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated overnight and were treated with 2 IU/ml hCG (Merck Sharp & Dohme B.V. ...
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bioRxiv - Cell Biology 2022Quote: ... Snap-Streptavidin-WA (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-Centrin-2 (1:250, clone 20H5, 04-1624, Merck Millipore), rabbit polyclonal anti-WDR90 (1:250 ...
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bioRxiv - Biochemistry 2020Quote: ... Grids were then stained with 2% (w/v) uranyl acetate pH 4 (Merck) for 1 min ...
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bioRxiv - Neuroscience 2021Quote: ... 2% B27 (v/v)) or BrightCellTM NEUMO photostable medium (1X NEUMO (Merck Millipore), 4% SOS ...
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bioRxiv - Cell Biology 2021Quote: ... A dose of 300mg/1kg body weight of APAP (Merck, 103-90-2) was injected i.p at a concentration of 40mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... 2ml/well of overlay {MEM containing 2% FBS and 0.4% Tragacanth (Merck, Israel)} was added to each well and plates were incubated at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Erythrocytes were removed from peripheral blood by Dextran sedimentation (2 % in PBS; Merck) and ACK lysis buffer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were grown in RPMI1640 supplemented with 2 mM glutamine (Merck, Darmstadt, Germany) and 10 % Fetal Bovine Serum (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 and 2 minutes respectively (Elastin van Gieson staining kit, Merck, Melbourne, Australia). For Ki67 and IBA1 staining ...
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bioRxiv - Microbiology 2021Quote: ... LB agar medium (Bacto) was prepared with 2% sodium carboxymethyl cellulose (CMC) (Merck) or 2% pectin (Agdia AG366 ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclei were stained with 4′,6-diamidin-2-phenylindol (DAPI, Merck, Germany).
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bioRxiv - Plant Biology 2021Quote: ... 0.5 g 2-(N-morpholino)ethanesulfonic acid (ULTRON grade, Merck KGaA, Darmstadt, Germany), and 5 g Gelrite (Duchefa) ...
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bioRxiv - Cancer Biology 2022Quote: ... The MNase reaction is stopped by adding 2 mM EGTA (Sigma-Aldrich/Merck). Afterwards ...
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bioRxiv - Biochemistry 2022Quote: ... transferred and tris-(2-carboxyethyl)-phosphin (TCEP, Merck, Sigma-Aldrich, 646547-10×1ML) added up to 1 mM and cysteine reduction performed for 30 min at 60°C ...
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bioRxiv - Microbiology 2022Quote: ... the samples were reduced by 1 mM tris(2-carboxyethyl)phosphine (TCEP, Merck) and free sulfhydryl groups carbamidomethylated using 5.5 mM chloroacetamide (Sigma-Aldrich) ...
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Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized micebioRxiv - Physiology 2023Quote: ... 400 µL of 1% 2-thiobarbituric acid (TBA; Merck, St. Charles, MO, USA), and 200 µL of 20% phosphoric acid and heated at 100 °C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... and 2 µl of pH 5 citrate-phosphate buffer excipient (Merck, Darmstadt, Germany); and endpoint B ...
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bioRxiv - Bioengineering 2022Quote: ... Compounds were separated on a C18 column (Merck Spherisorb ODS-2 (5 μm), 250×4 mm ...
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bioRxiv - Physiology 2024Quote: ... in trypsin (1mg/ml, Merck, UK, rocked at 4°C for 2 hours) followed by collagenase (type IV ...
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bioRxiv - Neuroscience 2024Quote: ... and 4′,6-diamidino-2-phenylindole (DAPI) (D9542) (all from Merck, Auckland, NZ). See table S2 for antibodies used for immunocytochemistry (ICC).
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were seeded in poly-HEMA (poly-2-hydroxyethyl methacrylate)-coated dishes (Merck) for embryoid bodies (EBs ...
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bioRxiv - Cell Biology 2023Quote: ... Nonspecific antibody blocking was for 1 h with 2% bovine serum albumin (Merck) in Tris-buffered saline ...
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bioRxiv - Cell Biology 2023Quote: ... they were supplemented with 2 µg/ml Hoechst 33342 (Merck, cat. No. B2261). All the antibodies used are listed in Supplemental Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant FGF-2 (10 ng/ml; Merck Millipore, Burlington, MA, USA) as mitogens ...