Labshake search
Citations for Merck :
1001 - 1050 of 2798 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... β-actin which was used as loading control was detected using a rabbit polyclonal IgG against mouse β-actin (A2103, Merck, Darmstadt, GER) diluted 1000-fold ...
-
bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain) was cloned into pET-45b(+) vector (Novagen/Merck, Darmstadt, Germay) via ‘in vivo assembly’ (35 ...
-
bioRxiv - Cell Biology 2022Quote: ... Membranes were also probed with either a mouse monoclonal antibody against GAPDH or a mouse monoclonal antibody against beta-actin (dilutions of 1:10,000 and 1:1000; Sigma-Aldrich/Merck group, USA) and with the IRDye® 680CW secondary antibody from Eurobio Ingen (926-32220 ...
-
bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were deparaffinized and rehydrated by standard methods. Thereafter, sections were incubated with monoclonal mouse anti-p53 (mutant, clone Ab-3 PAb240 catalogue no. OP29-200UG) (Merck, Darmstadt, Germany) diluted 1:100 in PBS at 4°C overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.
-
bioRxiv - Bioengineering 2024Quote: ... The scaffold was then rinsed three times with PBS and incubated with Alexa Fluor™ 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) and CF™ 633 conjugated secondary donkey anti-rat IgG antibody (1:100 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The membrane was rinsed with TBS buffer and incubated with the primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) (1:2,500 ...
-
bioRxiv - Microbiology 2024Quote: ... Infection foci were visualised using the appropriate secondary antibody coupled with horseradish peroxidase (HRP; 1:5000, Sheep Anti-Mouse IgG A5906, Sigma-Aldrich, Merck, Germany) and TrueBlueTM chemical (SeraCare ...
-
bioRxiv - Microbiology 2024Quote: ... Actin was detected with monoclonal mouse antibody coupled with horseradish peroxidase (HRP; 1:75,000; A3854, Sigma-Aldrich, Merck, Saint-Quentin Fallavier, France). Membranes were exposed to the appropriate peroxidase-conjugated secondary antibodies (1:5000 goat anti-guineapig IgG A108P or 1:10000 sheep anti-mouse IgG A5906 ...
-
bioRxiv - Cell Biology 2020Quote: ... Cell diameters were measured using the Scepter 2.0 Cell Counter and Scepter Software Pro (Merck KGaA, Burlington, MA, USA), according to the manufacturer’s recommendations.
-
bioRxiv - Immunology 2020Quote: ... Cells were washed with PBS prior to cell lysis and immunoblotting with the anti-puromycin 12D10 antibody (Merck Millipore). For flow cytometry (flow ...
-
bioRxiv - Cell Biology 2021Quote: Cell stress was induced by culturing the cells in a medium containing 500 μM arsenite sodium (Merck, Darmstadt, German) for 30 min ...
-
bioRxiv - Immunology 2023Quote: ... single-cell suspensions were depleted of red blood cells (RBCs) by using RBC lysis buffer [0.15 M NH4Cl (Merck), 1 mM KHCO3 (Merck) ...
-
bioRxiv - Biophysics 2024Quote: ... Whole cell lysates are prepared using the Cell Lysis Buffer (Cell Signalling Technologies) according to the manufacturer’s protocol supplemented with PMSF and Protease Inhibitory Cocktail (Merck). Protein estimation was done using the Bradford’s method using Bradford reagent (Amresco ...
-
bioRxiv - Immunology 2023Quote: ... RH5ΔNC-specific B cells were identified as live CD19+ IgG+ RH5ΔNC-APC+ RH5ΔNC-PE+ cells and single cell sorted into 96-well plates containing 10µL/well lysis buffer (10mM Tris [T3038, Merck] ...
-
bioRxiv - Cell Biology 2024Quote: ... stimulator cells (0.5 × 106 cells per well) were treated with mitomycin C (50 μg/mL; Sigma-Aldrich; Merck Millipore) for 30 min at 37 °C in a 24-well plate ...
-
bioRxiv - Cell Biology 2024Quote: ... and the cell pellets (1.5 x 106 cells) were lysed with 150 µL of 1× RIPA buffer (Merck Millipore), kept on ice ...
-
bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies (as appropriate) were added for 1 hour at room temperature (goat anti-rabbit/ goat anti-mouse, IgG, HRP conjugated; Merck Millipore (1:5000)) before visualisation using enhanced chemiluminescence (1.25 mM luminol ...
-
bioRxiv - Plant Biology 2021Quote: ... the sections were incubated in 1× PBS containing 1% (1:100 dilution) mouse monoclonal anti-Flag M2 primary antibody (Merck, cat. no. F1804) and 0.1% (w/v ...
-
bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
-
bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
-
bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
-
bioRxiv - Neuroscience 2021Quote: ... unbound primary antibodies were removed by washing in TBST and the blots were incubated with HRP-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG and IgM HRP conjugated antibodies (1:4000, Merck EMD Millipore, Ap130P), or goat anti-rabbit IgG antibody ...
-
bioRxiv - Biochemistry 2022Quote: ... Blocked membranes were incubated (1 hour, 1/5000 dilution, RT) with mouse anti-phosphotyrosine (clone 4G10) directly conjugated to HRP (4G10-HRP; Merck/ Millipore, RRID: AB_310779), or unlabelled 4G10® Platinum (Merck/ Millipore ...
-
bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
-
bioRxiv - Microbiology 2023Quote: ... the cells were stained with goat anti-rabbit Alexa Fluor® 555 (Thermo, 1:1,000) and mouse anti-IE Alexa Fluor® 488 (Merck, 1: 1,000) for 1 hour at RT ...
-
bioRxiv - Genomics 2024Quote: ... with the following primary antibodies: goat anti-IL1RN (RD Systems Cat # AF280NA) in 1:1000 in blocking solution and mouse anti-β actin (MERCK Cat # A1978) and rabbit anti-vinculin (Cell Signaling Cat #18799 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The plate was washed three times with TBS-C blocking solution and incubated with goat-anti mouse IgG-HRP (A4416, Merck; dilution 1/10,000) added in TBS-C blocking solution and incubated during 1 h at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... Coverslips were placed in a humid chamber and cells were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: mouse monoclonal anti-FLAG clone M2 (Merck F1804; 1:1000), rabbit monoclonal anti-KTN1 clone D5F7J (Cell Signaling Technology #13243 ...
-
bioRxiv - Cell Biology 2020Quote: ... ChIP assays were performed using a specific kit (Merck Millipore, Darmstadt, Germany). After crosslinking ...
-
bioRxiv - Cell Biology 2020Quote: PP2A activity were measured by PP2A phosphatase activity assay kit from Merck Millipore (PP2A immunoprecipitation phosphatase assay kit ...
-
bioRxiv - Cancer Biology 2020Quote: ... the ApopTag® Red In Situ Apoptosis Detection Kit (S7165, Merck Millipore) was also used to label apoptotic cells following Wells and Johnston protocol (Wells and Johnston ...
-
bioRxiv - Cell Biology 2021Quote: RIP assays were performed using a Magna Nuclear RIP Kit from Merck Millipore (17-10520 ...
-
bioRxiv - Cell Biology 2021Quote: The Magna RNA-binding protein immunoprecipitation kit (Merck Millipore, Billerica, MA, USA) was applied for the assay ...
-
bioRxiv - Cell Biology 2021Quote: Co-IP was performed using Protein G Immunoprecipitation Kit (Merck, Cat # IP50) as per the manufacturer's protocol ...
-
bioRxiv - Molecular Biology 2022Quote: PLA detection was performed using a Duolink In Situ Orange Kit (Merck) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: We performed the proximity ligation assay129 using the Duolink PLA Kit (Merck) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2022Quote: ... RNA was purified using the GenElute Mammalian total RNA Miniprep kit (MERCK). Samples from cell culture were harvested in the RNA extraction buffer supplemented with 10mM ß-ME ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting RNA was then subjected to amplification (Merck WTA2 Amplification Kit) to produce cDNA that was subsequently purified by column capture (Qiagen QiaQuick PCR purification kit ...
-
bioRxiv - Immunology 2021Quote: QCM Boyden chamber chemotaxis neutrophil migration assay kits were purchased from Merck Millipore (ECM505 ...
-
bioRxiv - Microbiology 2021Quote: ... KOD Xtreme Hotstart DNA polymerase kit was obtained from Merck (Darmstadt, Germany). T4 DNA ligase was obtained from New England Biolabs (NEB) ...
-
bioRxiv - Immunology 2022Quote: ... or with vaccine RNAs using RiboJuice™ mRNA Transfection Kit (Merck Millipore) according to the manufacturer’s instructions and incubated for 18 h ...
-
bioRxiv - Developmental Biology 2024Quote: Proximity ligation was performed using Duolink PLA assay kit (Merck, Cat. #DUO92102) according to the manufacturer’s guidelines ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... the ApopTag Plus Fluorescein In Situ Apoptosis Detection Kit (Merck, Darmstadt, Germany) was used according to the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... QPCRs were performed using a KAPA SYBR FAST kit (Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... stained with haematoxylin and eosin (H&E) using a kit (Merck, Germany) and myotube diameter measured by light microscopy analysis of DIC captured images (Olympus CKX41 ...
-
bioRxiv - Genetics 2024Quote: ... plasmid DNA was extracted (GenElute HP Plasmid Midiprep Kit, Merck #NA0200-1KT) from all of the E ...
-
bioRxiv - Cancer Biology 2024Quote: ... The IHC Select® HRP/DAB 150 kit (Merck KGaA, Darmstadt, Germany) was used as the secondary antibody to detect colour development.