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Citations for Merck :
1001 - 1050 of 4853 citations for 6 Amino 3 morpholin 4 ylmethyl indazole 1 carboxylic acid tert butyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: Zygotes were de-jellified using 3 % cysteine (Merck Millipore, USA), washed 6 times in N ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA oligonucleotide G4A4 (5’-AAAAAAGGGGAAAAGGGGAAAAGGGGAAAAGGGGAAAAAA-3’) was purchased from Merck. CD analysis of 2,5 µM RNA was carried out in the buffer used for G4-pulldown ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue slides were blocked with a 3% BSA (Merck, A7906) and 10% Donkey serum (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... peroxidase quench with 3% hydrogen peroxide (Merck, New Jersey, USA), protein block with Dako protein block solution (Dako ...
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bioRxiv - Cell Biology 2023Quote: ... in 2× saline sodium citrate (SSC; Merck, 6132-04-3)) for 5 min at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... Casein substrate (CAS: 9005-46-3) was bought from Merck and EnzChek casein BODIPY from Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... an inhibitor of the HIS3 reporter gene (3-AT; Merck), were added to avoid leakage of the HIS3 gene (Castrillo et al. ...
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bioRxiv - Bioengineering 2024Quote: ... mouse anti-Glyceraldehyde 3-phosphate dehydrogenase (GAPDH)-peroxidase (69295, Merck); anti-rabbit immunoglobulin G (IgG) ...
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bioRxiv - Genetics 2024Quote: ... 33 mM 1,2-diheptanoylsn-glycero-3-phosphocholine (DHPC by Merck) and 1% w/v sodium deoxycholate (Sigma) ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed for 1 min in 4% formaldehyde and stained for AP activity using the Alkaline Phosphatase Detection Kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Plant Biology 2020Quote: About 8,000 T4 seeds from the W29-1-4 reporter line were mutagenized by treatment with 0.3% (v/v) ethylmethanesulfonate (Merck KGaA, Frankfurter, Darmstadt, Germany) for 15 h at 25°C ...
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bioRxiv - Neuroscience 2019Quote: ... cortical sections were incubated overnight at 4 °C with anti-glial fibrillary acidic protein (GFAP, 1:1000, chicken IgG, Merck Millipore, Burlington, EUA), anti-Iba1 (1:200 ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were also stained with antibodies against the syncytiotrophoblast layer II marker monocarboxylate transporter 4 (MCT4; Merck Millipore AB3314P, used at 1:100) and against E-cadherin (CDH1 ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore, Billerica, Massachussetts) with the addition of 2,5% 1,4-Diazobicyclo[2.2.2]octan (Dabco ...
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bioRxiv - Immunology 2023Quote: ... The embryos were subsequently incubated overnight at 4 °C in solution containing Cy3-conjugated α-smooth muscle actin monoclonal antibody (1:100, C6198; Merck, Darmstadt, Germany), Alexa647-Phalloidin (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... slick SSW and non-slick SSW from sites #1 – #3 (Fig. 1b) were fixed with 25 % glutardialdehyde (0.5 % final concentration, Sigma-Aldrich/Merck Life Science AB, Solna, Sweden) and stored at −80 °C ...
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bioRxiv - Immunology 2024Quote: ... we included αTIM-3+ αPD-1 and used anti-human RSV-IgG4 as an isotype control antibody (5 μg/mL, 60AGK S228P, Merck & Co., Inc., Rahway, NJ, USA). On day six ...
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bioRxiv - Developmental Biology 2022Quote: ... GHCre/iDTR mice and Cre-negative control littermates (further referred to as -/iDTR) (postnatal day (PD) 4) were intraperitoneally (i.p.) injected with 4 ng diphtheria toxin (DT; Merck, Darmstadt, Germany) per g bodyweight ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto self-made 4–13% or 4-10% polyacrylamide gradient gels separated and transferred onto PFDV membranes (Merck Millipore) followed by detection by specific antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Unencapsulated molecules were removed by washing at least 4 times the LPs suspensions with PBS pH 7.4 for 20 min at 4°C and 5000 x g using an Amicon Ultra-4 concentrator with 100 kDa cutoff (Merck Millipore). The degree of encapsulation was calculated by subtracting from the total mass of the molecule to be encapsulated that present in the flow-through and was found to be higher than 75% in all LP preparations.
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bioRxiv - Cancer Biology 2024Quote: ... or expressing the sequence of 4-1BBL (Vector Builder, pLV[Exp]-Hygro-EF1A>4-1BBL) using X-tremeGENE™ HP DNA Transfection Reagent (Merck).
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were permeabilized with 0.01% saponin (10 min, 4°C) and labeled with Phalloidin-TRITC (Merck, 30 min, 4°C). After washing ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells seeded in 6-well plates or 100mm dishes were transfected using GeneJuice (Merck) and 1 to 3µg DNA ...
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bioRxiv - Cell Biology 2021Quote: 6 μL of 70 kDa FITC-dextran in EGM-2 (5 mg/mL, Merck, #46945) was added to each vessel ...
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bioRxiv - Plant Biology 2019Quote: ... leaf samples were harvested with a 6-mm-diameter cork borer (Z165220; Merck-Sigma-Aldrich), resulting in leaf discs with an area of 0.283 cm² ...
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bioRxiv - Physiology 2022Quote: ... Food contained low melting agar (OmniPur® Agarose, Low Melting, CAS 9012-36-6, Merck) during lifespan assessments only to ensure the quality of compounds was preserved ...
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bioRxiv - Microbiology 2022Quote: ... The sample was then injected into a Superose® 6 Increase 10/300 GL (Merck) column ...
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bioRxiv - Neuroscience 2022Quote: ... Agarose 0.6% brain phantoms50 were prepared by dissolving agarose (A9539, CAS 9012-36-6, Merck) in tris-borate-EDTA buffer 1X (T4415 ...
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bioRxiv - Cancer Biology 2020Quote: ... and further filtered to a final volume of 1 ml by repeated centrifugation (4000 × g, 4°C) using an Amicon Ultra-15 spin-filter unit (Merck Millipore, MWCO = 100 kDa). The sample was then loaded onto a 24 ml SEC column packed with sepharose CL-4B ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The staining of the precipitated polypeptide-antibody complexes was performed by addition of 60 mg 4-chloro-1 naphtol (Merck/Sigma-Aldrich; Cat#C8890) in 20 ml ice-cold methanol to 100 ml phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... purified DNA from both input and ChIP fractions was diluted 1:4 in water and 1 μL was used for qPCR using a SYBR® Green JumpStart™ Taq ReadyMix™ (CAT S4438, Merck, UK) and a BioRad CFX96 instrument ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein stain (30% methanol, 6.7% acetic acid, 0.0005% Fast Green FCF (Merck KGaA, Darmstadt, Germany)) stain was used as a loading control and imaged prior to blocking ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA:DNA hybrids were immunoprecipitated from total nucleic acids using 5µg of S9.6 antibody (MABE1095, Merck Millipore) in binding buffer (10mM Na2HPO4 pH 7.0 ...
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bioRxiv - Systems Biology 2021Quote: ... 100 μl of glass beads (0.5 mm, acid-washed, cat no. G8772, Merck KGaA, Darmstadt, Germany) were added to each well using a 96-well bead dispenser (LabTIE International ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide fractions were eluted using MeOH buffer containing trifluoroacetic acid (TFA) (Merck Sigma, Cat. No. 302031), FA or ABC ...
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bioRxiv - Neuroscience 2021Quote: 12 wells plates were coated o/n with poly-L-lysine (0.1 mg/ml [SigmaAldrich]) in 0.1 M borate buffer pH 8.5 (0.04 M Boric acid [Merck Millipore] ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Developmental Biology 2020Quote: ... Signal was developed using an ECL substrate (100 mM Tris-HCl, pH 8.5, 0.2 mM coumaric acid, 1.25 mM luminol, 0.01% H2O2; all Merck) and exposed on X-ray films (Agfa) ...
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bioRxiv - Cell Biology 2019Quote: ... fixed with acetic acid in methanol and stained with Coomassie brilliant blue G250 (EZBlue, G1041, Merck) for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... The samples were deproteinized by addition of 10 μL of cold 5-sulphosalicilic acid (SSA, Merck) (300 mg/ml ...
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bioRxiv - Plant Biology 2020Quote: ... Analytical grade zinc acetate dehydrate (Zn(CH3COO)2.2H2O) and citric acid (C6H8O7) were purchased from Merck and used as received ...
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bioRxiv - Neuroscience 2021Quote: Commercial pectin derived from citrus peels (galacturonic acid, ≥74.0%, dried basis, Sigma-Aldrich, Merck, Darmstadt, Germany), labelled “Citrus” in Figures ...
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bioRxiv - Bioengineering 2022Quote: ... the sections were rinsed in 0.1% acidified water and 0.5% acidified water (Acetic acid, Merck, 1.00056.2500) each for 1 minutes ...
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bioRxiv - Pathology 2023Quote: ... the ECM samples were treated with 0.18 % peracetic acid solution (32% w/w; Merck, Darmstadt, Germany) in 4.8% ethanol (Fresenius Kabi ...
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bioRxiv - Cell Biology 2023Quote: ... and an equal number of cells were digested in 100 μl of Nitric acid (Merck #1.00441.1000) overnight at 95°C ...
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bioRxiv - Plant Biology 2023Quote: ... AIR was extracted sequentially first with 50 mM CDTA (1,2-Cyclohexylenedinitrilotetraacetic Acid) (CDTA; Merck Darmstadt, Germany) followed by 4 M NaOH ...
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Class IIa HDACs inhibit cell death pathways and protect muscle integrity in response to lipotoxicitybioRxiv - Cell Biology 2023Quote: ... washed twice with 95% ethanol and resuspended in acetate buffer (0.84% sodium acetate, 0.46% acetic acid, pH 4.75) containing 0.3mg/mL amyloglucosidase (Merck). Glycogen was digested overnight at 37°C before glucose content was quantified using the glucose oxidase method15 and 14C-glucose incorporation was measured ...
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bioRxiv - Bioengineering 2024Quote: ... the gelatin was first removed from the solution by precipitating it with trichloroacetic acid (Merck, T4885). The content of sodium was calculated using a calibration curve made by measuring dilutions of a standard sodium solution (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... The loading pump supplied a solution of 0.1% trifluoroacetic acid in 98% water/acetonitrile 2% (Merck) at 3 µL/min ...