Labshake search
Citations for Merck :
951 - 1000 of 1763 citations for Progesterone Metabolites ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... Reaction progress was monitored with analytical thin-layer chromatography (TLC) on aluminum backed precoated silica gel 60 F254 plates (E. Merck). Column chromatography was carried out using silica gel 60 (230-400 mesh ...
-
bioRxiv - Immunology 2022Quote: ... The mix was transferred to a plate coated with avidin and the FLAG-peptide was detected with an anti-FLAG-alkaline phosphatase conjugate (Merck), developed with pNPP SigmaFAST (Merck ...
-
bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 2-week-old seedlings grown on plates under 16-hour photoperiod using RNAzol reagent (Sigma-Aldricht, Merck) according to the manufacturer’s instructions and DNA precipitation with a 4-fold volume of bromoanisole ...
-
bioRxiv - Plant Biology 2022Quote: ... Five μl of each reaction mixture were loaded onto a TLC plate (TLC Silica gel 60 F254, Merck, Darmstadt, DE). The mobile phase was 7 vol-parts of 1-propanol mixed with 8 vol-parts of H2O ...
-
bioRxiv - Plant Biology 2022Quote: ... The purity of compounds was checked using 1H NMR or thin layer chromatography (TLC) using pre-coated aluminium-backed plates (silica gel 60 F254, Merck) and compounds were visualised by UV radiation at 254 nm and then using an anisaldehyde spray reagent (1% p-anisaldehyde:2% H2SO4 ...
-
bioRxiv - Microbiology 2022Quote: ... The reactions were centrifuged for 5 min at 17000 × g and 20 µl of the supernatant was analyzed by TLC on a HPTLC alumina silica gel 60 plate (Merck) using 2-butanol-pyridine-ammonia-water (39:34:10:26 ...
-
bioRxiv - Immunology 2023Quote: ... Cells were transferred to plates, and then washed in 100-200 μL FACS buffer (PBS containing 2% FCS, 2 mM EDTA (Merck) and centrifuged at 400 x g for 5 mins ...
-
bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed with centrifugation and 2+2 μl of extracts and a commercial pinosylvin standard (ArboNova) were applied on TLC plates Silica gel 60 F254 (Merck), which were developed with chloroform ...
-
bioRxiv - Microbiology 2023Quote: Time-lapse microscopy analyses were performed in a liquid medium using a CellASIC ONIX platform and compatible B04A plates (Merck), as described previously (Hołówka et al ...
-
bioRxiv - Biochemistry 2023Quote: ... The residue was suspended in 10 µL of n-butanol saturated in water and chromatographed on silica 60 plates (20×20 cm, Merck). Plates were developed for 7-10 cm with isopropyl alcohol/ammonia (32%)/water (6:3:1 by volume) ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were taken up in MeOH and spotted onto an activated thin layer chromatography (TLC) silica gel 60 plate (Merck). The plate was developed in CHCl3:MeOH:Acetic Acid:H20 (25:15:4:2 ...
-
bioRxiv - Bioengineering 2023Quote: ... The pre-adipocytes were plated in custom-made plates (Supp. Fig. 15b) and cultured in growth medium -low glucose (1g l-1) DMEM (Merck) supplemented with 10% FBS (Merck) ...
-
bioRxiv - Biochemistry 2023Quote: Cancer cells were seeded at a density of 1,000 cells/ 100 μL complete medium into 96-well cell culture plates (#655180, Greiner bio-one, Merck KGaA). After 24 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was added to Fe-NTA agarose beads (PureCube) on a multiscreenHTS-HV 0.45 μm 96 well filter plate (Merck Millipore). After loading ...
-
bioRxiv - Microbiology 2023Quote: ... The whole sample was applied to a high-performance thin-layer chromatography (HPTLC) silica gel 60 plate (10 x 10 cm; Merck) with a Hamilton syringe ...
-
bioRxiv - Molecular Biology 2023Quote: ... HeLa cells grown on 15 cm plates (∼90% confluent) were treated either with 100 μg/mL cycloheximide (CHX, Merck, C1988) for 15 min or with 50 μg/mL puromycin (Puro ...
-
bioRxiv - Microbiology 2023Quote: ... these cultures were diluted 100x in the appropriate medium and either spotted on a sealed agarose pad (MOPS medium, 2% agarose, Figure 1D-F & 4A) or loaded in a CellASIC Onix plate (Merck) and perfused with MOPS medium containing 0.4% arabinose and 20 μg/ml chloramphenicol at 34.5 psi (Figure 3A & 5C) ...
-
bioRxiv - Microbiology 2023Quote: R47 and R32 wild type strains were inoculated and grown in 6-well plates as described in the RNA extraction section except that 1 ml 0.38 M NaOH (Merck, Germany) was added to the middle two wells to capture HCN in the surrounding ...
-
bioRxiv - Microbiology 2023Quote: ... Lipid extracts were then applied to silica gel high-performance thin-layer chromatography (HPTLC) plates (silica gel 60 F254, Merck) using a Linomat 5 sample application unit (CAMAG) ...
-
bioRxiv - Bioengineering 2023Quote: ... The hydrolytic products were separated by thin layer chromatography (TLC) on Partisil K6 Silica gel 60 plates (250 µm thickness, 20 × 20 cm; Merck) with developing solvent ...
-
bioRxiv - Cell Biology 2023Quote: ... we seeded cells at 2 * 106 cells/ mL in S2 media in 2 mL media/ well in a 6-well plate with either DMSO (D2650, Merck), 2.5 μM CytD (C2618 ...
-
bioRxiv - Biochemistry 2023Quote: ... The reactions were stopped by separating the enzyme and soluble products from the insoluble chitin substrate by filtration using 96-well filter plates (Merck). The concentration of oxidized LPMO products was also determined in aliquots taken from the pre-incubation reaction with β-chitin to allow accounting for carry-over of products potentially formed in the pre-incubation phase.
-
bioRxiv - Cell Biology 2023Quote: HPAECs were seeded at the density of 10,000 cells/well in a pre-prepared 96-well plate containing 30μl of growth-factor reduced Matrigel (Merck, # CLS354230-1EA) per well (38) ...
-
bioRxiv - Biochemistry 2023Quote: ... equivalent masses of total lipid fraction from each strain were spotted onto a high-performance TLC (HPTLC) silica gel 60 plate (Merck) with a Camag ATS4 apparatus ...
-
bioRxiv - Cell Biology 2024Quote: Samples were resuspended in 6 μL 40% (v/v) 1-propanol with vortexing and bath sonication were applied to Silica Gel 60 aluminium-backed HPTLC plates (MERCK) (30 ...
-
bioRxiv - Immunology 2023Quote: ... difficile cultures were grown in TY broth and sub-cultured to an Optical Density at 600 nm (OD600nm) of 0.05 in 200 μL of BHISG in 96-well flat bottom plates (Merck, #Z707902). Bacterial growth was followed for 24h or 18h with OD600nm measurements every 30 min using GloMax Plate Reader (Promega) ...
-
bioRxiv - Microbiology 2023Quote: ... Conidia from 14-day-old fungal cultures grown on culture media agar plates were filtered through two layers of Miracloth (Merck) and incubated with Hoechst 33342 solution (20 mM ...
-
bioRxiv - Cancer Biology 2023Quote: Tumorspheres formation assay was performed by seeding transfected cells in Corning Costar ultra-low attachment plates (#CLS3473, Merck, Madrid, Spain) in DMEM F-12 medium (#11320033 ...
-
bioRxiv - Biochemistry 2023Quote: ... Reactions were monitored by thin-layer chromatography on commercially available silica pre-coated aluminium-backed plates (Merck Kieselgel 60 F254). Visualisation was under UV light (254 nm and 366 nm) ...
-
bioRxiv - Microbiology 2023Quote: Polar lipids were separated by 2D-TLC by spotting 200 μg of total lipids on 60 F254 silica gel plates (Merck) using chloroform ...
-
bioRxiv - Microbiology 2024Quote: Extracted lipid (dried organic mixture) was solubilized in chloroform and 4µg was resolved by 2D TLC on aluminum-backed silica gel plates (Merck, India) by spotting the extract using microcapillary pipettes as described Pirson et al. ...
-
bioRxiv - Immunology 2024Quote: 1×106 of splenocytes or lymph node cells or primary B cells were added to 24-well plates and were stimulated with the TLR4 agonist LPS (L6529, Merck), the TLR7 agonist Imiquimod (HY-B0180 ...
-
Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... the MSA agar plate was flooded and 500 µg/ml of nalidixic acid (Himedia) and 1 mg/ml of apramycin (Merck). After incubation ...
-
bioRxiv - Neuroscience 2024Quote: ... Neurons were collected in Eppendorf tubes by disrupting adhesion to the plate with a jet of medium and washed twice in ice-cold PBS with Ca2+ and Mg2+ (Merck), supplemented with complete ...
-
bioRxiv - Cell Biology 2023Quote: For the thin layer chromatography (TCL) an aliquot of lipid extract corresponding to 8 mg of DCW was applied to silica gel TLC plates (Merck) by a Linomat 5 semiautomatic sample applicator (Camag) ...
-
bioRxiv - Immunology 2024Quote: ... Neutralized bovine collagen I (2 mg/ml) was plated 40 µl per well in a black 96-well plate with flat clear bottom (Corning/Merck). The plate was kept at 37°C with 5 % CO2 for 1 hour ...
-
bioRxiv - Immunology 2024Quote: ... K562-pCasper target cells were resuspend in neutralized bovine collagen I (2 mg/ml) and plated in a black 96-well plate with flat clear bottom (Corning/Merck) at a density of 25,000 cells/40 µl per well ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2958 bp) was purified using commercial DNA purification kits (GenElute™ HP Plasmid Miniprep kit, Merck, #NA0160), according to standard procedures ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5 mM MgCl2) before addition of Laemmli buffer with 0.25 U/µL benzonase (final concentration, Merck Millipore) for 10 min followed by 5 min denaturation at 95°C.
-
bioRxiv - Developmental Biology 2019Quote: ... Membranes were blocked with 5% non-fat milk in PBS with 0.05% Tween-20 (Merck; blocking buffer) and incubated with primary antibodies (Table S1 ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... we used a slightly modified version of the iron-limited CAA medium: 5 g Casein Hydrolysate (Merck), 0.25 g MgSO4*7H2O (Sigma-Aldrich) ...
-
bioRxiv - Bioengineering 2021Quote: ... media supplemented with 100 µg/mL FITC-dextran with sizes of 3-5 kDa (FD4; Merck KGaA) or 40 kDa (FD40 ...
-
bioRxiv - Microbiology 2020Quote: ... pH 7.2) and purified by filtration through 5 μm pore polycarbonate membranes (Merck Millipore Co. Cork, Ireland).
-
bioRxiv - Cancer Biology 2022Quote: PEGylated-poly T coated coverslips were assembled as described and further passivated with 5% BSA (Merck, A7906) for 30 minutes followed by wash with IMB ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were washed three times with PBS and blocked with 5% normal donkey serum (NDS) (Merck-Millipore), 0.1% Triton X-100 in PBS for 1 h at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... supplemented with 2.5 µM IWP4 (Stemgent, Cambridge, MA, USA) and 5 µM XAV939 (Merck, Kenilworth, NJ, USA). The culture medium was refreshed with RPMI□+□B27+insulin medium every other day ...
-
bioRxiv - Cell Biology 2022Quote: Depletion of Cav1 was achieved by RNAi using siRNAs with the following sequence: 5’ GCAUCAACUUGCAGAAAGA 3’ (Merck), and Control siRNA Luciferase ...
-
bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared via centrifugation and loaded on two 5 mL HiTrap Heparin HP (Cytiva, Merck) columns (connected in series ...
-
bioRxiv - Neuroscience 2022Quote: Conditioned media from astrocytes were collected at 5 DIV and filtrated with Millex-GP filter (SLGPR33RS, Merck). ACM was concentrated with Amicon Ultra 10kDa (UFC501008 ...
-
bioRxiv - Cell Biology 2022Quote: ... at a final concentration of 5 μg/ml in M2 medium or Latrunculin B (428020-1MG, Merck) at a final concentration of 5 μM in M2 medium (to disrupt F-actin) ...