Labshake search
Citations for Merck :
951 - 1000 of 1044 citations for Mouse Junction Plakoglobin JUP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... Total protein concentration of lysates was determined using a bicinchoninic acid kit (Merck Life Science UK Ltd, Gillingham, UK) and samples diluted in Laemmli buffer ...
-
bioRxiv - Immunology 2024Quote: ... For FACS sorted cells RNA was amplified cDNA synthesized using a SeqPlex RNA Amplification kit (Merck Life Science, UK) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... 2 and 3 were detected with another multiplex assay (MILLIPLEX MAP TGFß Magnetic Bead 3 Plex Kit; Merck Millipore).
-
bioRxiv - Immunology 2023Quote: ... All cell lines were routinely tested for mycoplasma contamination using LookOut Mycoplasma qPCR Detection Kit (Merck 200-664-3). None of the cell lines were authenticated but low passage numbers were utilized.
-
bioRxiv - Immunology 2023Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore). Immunoprecipitation of the library was performed in accordance with a previously published protocol7,11 ...
-
bioRxiv - Biophysics 2023Quote: The aqueous solutions of GQDs were purified utilizing dialysis kits and Amicon ultra-filtration devices (Amicon Ultra-15, Merck) with 1 and 3 kDa membranes ...
-
bioRxiv - Microbiology 2024Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore) using Liga5™ (A&A Biotechnology ...
-
bioRxiv - Cancer Biology 2021Quote: ... Counterstaining of nucleus was performed using Mayer’s hemalum solution and slides were mounted with Kaiser’s glycerol gelatin (the Kit and all reagents were obtained from Merck Millipore). Images were acquired as described above.
-
bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
-
bioRxiv - Molecular Biology 2019Quote: ... RIP-Seq experiments with UV cross-linking were performed using the Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (17-700 Merck). Briefly ...
-
bioRxiv - Molecular Biology 2020Quote: ... Three individual clones were picked and amplified in LB broth including antibiotics for 12 h at 37°C and isolated using GenElute– HP Plasmid MaxiPrep Kit (Merck). Integrity of purified expression constructs was validated by gel electrophoresis and the concentration was determined by absorbance measurement ...
-
bioRxiv - Cell Biology 2021Quote: In vitro PI3 Kinase activity measurements were performed with the PI3K Kinase Activity/Inhibitor assay kit following manufacturer’s instructions (Merck Millipore). Briefly ...
-
bioRxiv - Microbiology 2021Quote: ... containing a knockout of DNA Ligase 4) 44 that had been made competent for DNA uptake using the LiCl2-based Yeast transformation kit (YEAST1-1KT; Merck). The transformed cells were plated on minimal synthetic defined (SD ...
-
bioRxiv - Plant Biology 2022Quote: ... obtained by grinding in liquid nitrogen and using a SpectrumTM Plant Total RNA kit (Sigma-Aldrich, Merck KGaA, Darmstadt, DE) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Cat# AS1107) for one hour at room temperature and visualized using the Immobilon ECL substrate kit (Millipore, Merck KgaA, Germany). Primary antibodies specific to PLLP (1:700 ...
-
bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
-
bioRxiv - Molecular Biology 2019Quote: The protocol for detecting SINEUP RBPs was based on the protocol for the Magna ChIRP RNA Interactome kit (Merck Millipore) with the modification of cross-linking with 300 mJ/cm of 264 nm UV light (CL-1000 Ultraviolet Cross Link ...
-
bioRxiv - Microbiology 2021Quote: Ago2 RIPs were performed on TREx-BCBL-1 RTA cells using EZ-Magna RIP RNA binding Immunoprecipitation Kit (Merck millipore) as per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... and sulfide were measured from the supernatant after centrifugation (10 min at 5000 rpm) using specific colorimetric chemical kits provided by Merck AG (Zug ...
-
bioRxiv - Immunology 2022Quote: ... an aliquot of the sample was taken for measuring the total protein concentration using a BCA assay kit (Merck Millipore). The remaining sample was heat-treated (70 °C ...
-
bioRxiv - Microbiology 2022Quote: ... containing a knockout of DNA Ligase 4) [64] that had been made competent for DNA uptake using the LiCl2-based Yeast transformation kit (YEAST1-1KT, Merck). The transformed cells were plated on minimal synthetic defined (SD ...
-
bioRxiv - Plant Biology 2023Quote: ... Total RNA was isolated from 100 mg of fresh root tissue sampled from three individual plants (biological replicates) using the Spectrum Plant RNA Purification Kit (Merck). RNA was treated with RNase-free DNase Set (Qiagen ...
-
bioRxiv - Molecular Biology 2022Quote: ... the final peak integration was done with the TraceFinder 4.1 software (Thermo Fischer Scientific) using confirmed retention times of 462 metabolites in-house library developed using library kit MSMLS-1EA (Merck). For further data analysis ...
-
bioRxiv - Molecular Biology 2024Quote: ... The resulting pellet was retained and subject to extraction following the RED Extract Plant PCR-Kit (Merck KGaA, Darmstadt, Germany) protocol.
-
bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from cultures that finished sexual development using GeneElute – Mammalian Genomic DNA Miniprep Kit (Merck-Sigma, Germany). PCRs were done on specific genomic regions flanking an IES (Table S6 ...
-
bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies (table 1) were added in 5% milk TBS-T and membranes were developed using ECL luminol kit (Merck) and chemiluminescence films (Amersham Hyperfilm ECL ...
-
bioRxiv - Cell Biology 2023Quote: ... TUNEL staining was made according to the manufacturer’s instructions of the apoptosis detection kit in situ red (S7165, Merck-Millipore).
-
bioRxiv - Molecular Biology 2022Quote: ... 1 µg of the digested RNA was reverse-transcribed as described above and validated by PCR using the KOD Xtreme HotStart Polymerase Kit (#71975-3; Merck, PCR program ...
-
bioRxiv - Developmental Biology 2023Quote: ... The reverse transcription reaction to obtain the cDNA was performed with the MystiCq® microRNA cDNA Synthesis Mix kit (Merck) and amplified by PCR using the following primers (miR-34-5p Fw ...
-
bioRxiv - Microbiology 2023Quote: The dissolved inorganic carbon concentration needed for the calculation40 was determined using the CaCO3 Merck Spectroquant kit (No. 1.01758.0001) and the Merck Spectroquant Pharo 100 spectrophotometer (Merck, Schaffhausen, Switzerland).
-
bioRxiv - Microbiology 2023Quote: ... Regular testing for mycoplasma contamination was performed to confirm contamination free culture using a PCR-detection kit (Sigma-Aldrich/Merck). The details of cell lines are provided in key resources table ...
-
bioRxiv - Microbiology 2023Quote: ... and peak integration was carried out with TraceFinder 4.1 software (Thermo Fischer Scientific) using confirmed retention times for 463 metabolites standardized with a library kit MSMLS-1EA (Merck). Peak smoothening was adjusted to 7 ...
-
bioRxiv - Plant Biology 2024Quote: ... Calibration of the column was performed using the Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da (#MWGF200; Sigma-Aldrich/Merck, www.sigmaaldrich.com).
-
bioRxiv - Plant Biology 2024Quote: ... a tissue sample (up to 100 mg fresh weight per macroalga) was used for genomic DNA extraction using the GenElute Plant Genomic DNA Miniprep Kit (Merck). Genotyping PCR was performed using Prime-STAR GXL DNA Polymerase (Takara ...
-
bioRxiv - Immunology 2024Quote: ... LDH activity was quantified in the cell lysate of untreated cells and in all cell supernatants using the Cytotoxicity Detection Kit (LDH) from Merck, following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... 6 x 103 hESCs were seeded on mitotically inactivated MEF feeder in a well of 12-well plates and cultured in hESC culture medium for five days before subjected to alkaline phosphatase staining using Leukocyte Alkaline Phosphatase Kit (Merck) following manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2024Quote: ... and a random RT-PCR amplification was performed using the TransPlex® Complete Whole Transcriptom Amplification Kit WTA2 (Merck Millipore) following the manufacturers’ instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... The medium was changed every two days for seven days before detection of alkaline phosphatase-positive colonies (Alkaline Phosphatase detection kit, Merck-Millipore). Alkaline phosphatase-positive colonies were analyzed and quantified using ImageJ analysis software (81).
-
bioRxiv - Molecular Biology 2020Quote: ... From the remaining nuclei RNA-IP was then undertaken with the MagnaRIP Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit protocol (17-700, Merck) from step I.3 ...
-
bioRxiv - Immunology 2021Quote: ... was used for m6A immunoprecipitation (m6A-IP) using an m6A antibody (Synaptic, 202003) following the standard protocol of the Magna MeRIP m6A Kit (Merck Millipore). RNA was enriched through RNA Clean & Concentration-5 (Zymo Research ...
-
bioRxiv - Plant Biology 2022Quote: A hole punch was used to obtain approximately 100 mg of leaf tissue from each sample (15 biological replicates per condition) and the total RNAs were extracted using the Spectrum Plant RNA kit (Merck, Spain). RNAs were quantified using the NanoDrop ND-1000 (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... and carbonic anhydrase (29 kD) from a combination of two kits (GE Healthcare, catalogue No. 28-4038-41, Chicago, Illinois, IL, USA and MERCK, Sigma Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) histochemistry was performed using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore), according to the instructions of the manufacturer ...
-
bioRxiv - Immunology 2019Quote: ... Cells were fixed with 4% (w/v) paraformaldehyde and the proximity ligation assay (PLA) was conducted using the Duolink® In Situ Kit (Merck) as per the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... 5-DIV cultured human spinal motor neurons and C5-C7 ventral horns of adult animals 3 days after surgery were estimated using a Rac1/Cdc42 Activation Assay Kit (#17-441, Merck Millipore). Briefly ...
-
bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... To access the activation of both PI3K and MAPK signaling pathways the Muse™ PI3K/MAPK Dual Pathway Activation Kit (Merck) was used ...
-
bioRxiv - Neuroscience 2022Quote: ... were used to assess the difference in beta-secretase activity in each anatomical region using the Beta-Secretase Activity Fluorometric Assay Kit (Merck, MAK237).
-
bioRxiv - Bioengineering 2022Quote: ... These oligonucleotides were used to create a phage library representing all oligopeptides using the T7Select 415-1 Cloning Kit (Merck Millipore). Immunoprecipitation of the library was performed in accordance with a previously published protocol (Harhala et al. ...
-
bioRxiv - Plant Biology 2022Quote: The m6A enrichment experiment was performed as described previously with minor modifications (Dominissini et al., 2013) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck) was used following the manufacturer’s instruction ...