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Citations for Merck :
951 - 1000 of 2125 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with anti-NeuN primary antibody (Merck, MAB377) and Alexa Fluor 488 goat anti-mouse IgG ...
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bioRxiv - Cell Biology 2024Quote: ... The mouse anti-alpha tubulin antibody (Merck, T9026; 1:10,000) was used as a loading control.
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bioRxiv - Evolutionary Biology 2021Quote: ... The pure proteins were concentrated using a 3000-high molecular weight cutoff centrifugal filter (Merck-Millipore). The concentrated proteins were then subjected to size-exclusion chromatography using a Superdex-75 GL column (GE healthcare pre-equilibrated with 25 mM Tris-HCl 6.8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein stain (30% methanol, 6.7% acetic acid, 0.0005% Fast Green FCF (Merck KGaA, Darmstadt, Germany)) stain was used as a loading control and imaged prior to blocking ...
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bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... downward capillary action was used to transfer protein to immune-blot PVDF membrane (Merck cat# IPVH00010). Membranes were blocked with 5% milk in wash buffer (0.05% Tween in PBS ...
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... Equal protein amounts were separated by SDS PAGE and blotted onto a polyvinylidene difluoride membrane (Merck) by using a Wet/Tank Blotting System (Biorad) ...
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bioRxiv - Immunology 2022Quote: ... Protein was digested by incubating membrane at 50°C for 1 h with proteinase K (Merck) diluted in PK-SDS buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein-containing fractions were dialyzed in a cellulose membrane tube (14 kDa MWCO, Merck-Millipore), first ...
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bioRxiv - Microbiology 2022Quote: Wild type and mutant CANC proteins were expressed in Escherichia coli Rosetta (DE3) cells (Merck Millipore) as previously described (Betancor et al. ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysis and membrane protein extraction was performed using the ProteoExtract Native Membrane kit (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... 500–1000 µg of total protein (estimated by Direct Detect® Infrared Spectrophotometer assay, Merck Millipore), were incubated overnight with 2 µg of antibody raised against an intracellular epitope in mouse NLGN1 (aminoacids 826 to 843 ...
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bioRxiv - Neuroscience 2021Quote: ... Purified protein was concentrated to 8 mg/mL using 30 kDa cut-off AMICON concentrators (Merck).
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Proteins of interest were detected using the following primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a PVDF membrane (0.45µm Immobilon®-P, Merck Millipore Ltd., VIC, Australia) at 100 V in chilled transfer buffer containing 25 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... Proteins were resolved on SDS–PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). The PVDF membranes were then blocked with 5% skim milk and sequentially incubated with primary and secondary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was concentrated to ~65 mM using a MWCO 30,000 Amicon Ultra Centrifugal Filter (Merck), flash-frozen and stored at −80 °C until use.
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bioRxiv - Microbiology 2020Quote: Viral proteins were separated by SDS-PAGE and transferred to polyvinylidene disulfide (PVDF) membranes (Merck Millipore). After blocking the membranes with 5% skim milk ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated using Amicon® Ultra-4 Ultracel®-10K (MWCO: 10.000 Da; Merck). Protein concentrations were quantified using the calculated molar extinction coefficient ε280 (29).
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bioRxiv - Cell Biology 2021Quote: ... Purified proteins were were concentrated > 60 μM using Amicon Ultra centrifugal filters (Merck; MWCO 3 kDa), rebuffered to 50 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... The collected protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut off and frozen at −80 °C.
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bioRxiv - Microbiology 2021Quote: ... Protein samples for mass spectrometry were purified and concentrated using ZipTipC-18 (Merck, Millipore, Darmstadt, Germany).
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bioRxiv - Microbiology 2021Quote: ... Protein signals on the membranes were detected with Immobilon Forte Western HRP substrate (Merck, Darmstadt, Germany) and the ChemiImager Ready (Alpha Innotec,San Leandro USA).
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bioRxiv - Biochemistry 2021Quote: ... Proteins were eluted with 50 μl of 100 μg/mL of 3X FLAG tag peptide (Merck) in solubilization buffer followed by acetone precipitation (overnight ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μg protein was added to spin columns (Pierce) containing anti-FLAG M2 affinity resin (Merck) and allowed to incubate rotating for two hours at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... the protein was previously filtered with 0.22 µM Millex Syringe filters (Durapore PVDF, Merck KGaA, Germany) in order to exclude the presence of potential protein oligomers or aggregates ...
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bioRxiv - Neuroscience 2023Quote: ... γ8 proteins were further concentrated using Amicon Ultra centrifugal filter with MWCO 3K (Merck Millipore UFC500324).
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bioRxiv - Biophysics 2023Quote: ... Eluted proteins were concentrated in a 10 kDa molecular weight cutoff (MWCO) centrifugal filter (Amicon, Merck).
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bioRxiv - Plant Biology 2023Quote: ... and proteins were blotted onto a 0.45 µm PDVF Immobilon-P membrane (Merck Millipore, ref. IPVH00010) after electrophoresis ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were concentrated by using the Amicon Ultra centrifugal device (NMWL: 3K) (Merck, Millipore Cat# UFC500324), and the concentration was determined by the Pierce BCA protein assay kit (Thermo Fisher Scientific Cat# 23225) ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck, Darmstadt, Germany), upon induction with 0.6□mM IPTG at 0.8 OD600 ...
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bioRxiv - Biophysics 2022Quote: ... The pure protein obtained was concentrated using a 3 kDa Amicon® Ultra Centrifugal Filter (Merck).
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto methanol pre-activated polyvinylidene fluoride (PVDF) membranes (IPFL00010, Immobilon-P; Merck-Millipore). Membranes transferred from TGX Stain-Free™ gels were imaged and quantified for posterior normalization with a ChemiDoc XRS+ (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd.), and the progeny virus stock was aliquoted and stored at −20°C until use.
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bioRxiv - Neuroscience 2024Quote: ... Equal protein amounts were separated by SDS PAGE and blotted onto a polyvinylidene difluoride membrane (Merck) by using a Wet/Tank Blotting System (Biorad) ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were washed first with 1× BugBuster® (10× Protein Extraction Reagent Novagen® Merck), then twice with water and once with 50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 μg of proteins were emulsified with an equivalent volume of Freund’s Incomplete adjuvant (Merck F5506) and injected post 21 days ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore; Cat. No. UFC900324) at 3500 rpm and 4°C ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... proteins were transferred to a 0.45 μm PVDF membrane (#IPVH85R; Sigma-Aldrich/Merck, Zwijndrecht, The Netherlands) using an electroblotting device (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa: Merck, Darmstadt, Germany) and stored at −20 °C in dialysis buffer (150 mM NaCl ...
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bioRxiv - Plant Biology 2022Quote: ... The separated proteins were transferred to polyvinylidene difluoride membranes (Immobilon-P; Merck Millipore, Burlington, MA, USA) and analyzed with the following antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... the chips were coated with fibronectin (Human Plasma Fibronectin Purified Protein, Merck, Schiphol-Rijk, The Netherlands) in sterile PBS (100 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (Amersham Hybond ECL; GE Healthcare, RPN303D, Merck, Darmstadt, Germany). After blocking in 5% (w/v ...
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bioRxiv - Synthetic Biology 2023Quote: ... the proteins were concentrated to 15 – 30 mg/ml concentration using Amicon Ultra Centrifugal filters (Merck) via short (2 – 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Merck Millipore, cat #IPFL00010). After blocking with Intercept TBS Blocking Buffer (LI-COR ...
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bioRxiv - Biophysics 2024Quote: ... The eluted protein was concentrated with a 15 ml 10000 MWCO spin filter (Merck Millipore Ltd.). The sample was centrifuged at 20.000 g for 15 min to remove precipitated protein prior to being loaded onto a HiLoad 16/600 Superdex 200 size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred on a PolyVinyliDene Fluoride (PVDF) membrane (Immobilon-P, Merck Millipore, Burlington, USA) using liquid transfer with 20 % methanol at 100V ...
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bioRxiv - Biochemistry 2024Quote: Optional concentration of protein samples using Amicon® Ultra centrifugal filters (Merck Millipore Ltd., Carrigtwohill, IRL) was performed prior to buffer exchange to reduce sample volume ...
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bioRxiv - Immunology 2024Quote: ... HLA-G2 proteins in PBS were concentrated by Amicon Ultra-15 (NMWL 10,000 Da) (Merck Millipore), and the flowthrough solution from protein concentration was used as a negative control (PBS ...