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Citations for Merck :
51 - 100 of 2128 citations for Tetratricopeptide repeat protein 14 TTC14 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 70% humidity) for 14 days and watered with either ultra-pure water (Merck Millipore, Burlington, USA), Peter’s fertilizer (ICL specialty fertilizers ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 10 to 20 minutes column incubation with 14 mL of ultrapure water (Merck Milli-Q) as the eluant for the final step ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Cell Biology 2021Quote: ... antibody complexes were purified by incubation of lysate with Protein A Sepharose® beads (Merck, GE17-5138-01) for 3h rotating at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Rabies injected brains were immunohistochemically stained using antibodies against the 2A linker protein (Merck/ Millipore, ABS31, 1:2000) found in tTA expressing cells ...
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bioRxiv - Cell Biology 2024Quote: ... RSV F-protein was detected using a mouse anti-RSV antibody used 1:5000 in casein (MAB8262, Merck), which was incubated at 37 °C ...
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bioRxiv - Microbiology 2023Quote: Purified proteins and native protein standard (Supelco-Merck) were loaded in 4-15% gradient mini protean precast native gel (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 14 days in RPMI 1640 (ccpro) supplemented with 10 % heat-inactivated human serum (Merck), 10 ng/ml IL-7 ...
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bioRxiv - Neuroscience 2021Quote: ... Detection of newly synthesized proteins was carried out by an anti-puromycin antibody (clone 12D10, mouse-monoclonal, MABE343; Merck), an anti-HuD antibody (ab96474 ...
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bioRxiv - Cell Biology 2021Quote: ... Virotrap particles containing protein complexes were purified in a single step using biotinylated anti-FLAG BioM2 antibody (#F9291, Merck) and Dynabeads MyOne Streptavidin T1 Beads (#65601 ...
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bioRxiv - Microbiology 2022Quote: ... The sections were then incubated overnight in PBS with 0.2% BSA and 0.05% Tween-20 with primary antibodies directed against SARS-CoV-2 Nucleocapsid Protein (1:500; mouse monoclonal, # ZMS1075, Merck); Iba1 (1 ...
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bioRxiv - Molecular Biology 2024Quote: ... immunoprecipitations performed by incubating with 8 μl Protein G Dynabeads (Novex) and 12 μl anti-FLAG antibody (F3165, Merck) per sample for 90 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... for normalization of protein loading following which western blotting was performed using an anti-HA antibody (H9658 Merck, UK). HA tagged protein bands were visualised using the LI-COR Odyssey Clx system with LI-COR secondary antibody (IRDye 800 CW anti-mouse ...
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bioRxiv - Molecular Biology 2022Quote: ... CAS-No: 7647-14-5) and ultrapure water from a Milli-Q system were supplied from Merck (Darmstadt, Germany).
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bioRxiv - Genetics 2021Quote: ... Prior to EMSAs the in vitro translations of Rbpj (WT) and mutant proteins were monitored by western blotting using an anti-Flag antibody (M5, Merck).
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The amount of puromycin incorporated into nascent peptides was then evaluated by Western blot analysis on 20 μg of proteins using anti-puromycin antibody (ref MABE343) purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Cell Biology 2023Quote: ... The amount of puromycin incorporated into nascent peptides was then evaluated by Western blot analysis on 20 μg of proteins using anti-puromycin antibody (ref MABE343) purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitations were performed as previously described7 using Protein G Dynabeads (LifeTechnologies) coupled to Myc 4A6 antibody (Merck Millipore, 05-724) or FLAG M2 monoclonal antibody (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylated SR proteins were detected using a mouse monoclonal α-pan-phosphoepitope SR-specific antibody (1H4, Merck, Catalogue-No.: MABE50) at 0.025 µg/mL ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated on 12% non-reducing SDS-PAGE gel and cysteine-dimedone protein adducts visualised by western blot analysis using 1:10,000 diluted rabbit anti-cysteine sulfenic acid primary antibody (Merck Millipore) and 1:10,000 diluted horseradish peroxidase conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) were added to the mix followed by incubation at room temperature for 2 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) were added and incubated at room temperature for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... VLP samples were separated by 10% or 14% SDS-PAGE later blotted to PVDF membrane (Immobilon®-FL, Merck Millipore) and blocked overnight with ODYSSEY® blocking buffer ...
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bioRxiv - Molecular Biology 2022Quote: Phosphorylation of Sam68 N-term and C-term were obtained by incubation with CDK1/Cyclin B (Merck, catalogue 14-450) at a Cdk1:Sam68 ratio of 5:1000 at 20 °C ...
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bioRxiv - Microbiology 2021Quote: ... Acid digestion was then performed for 3 h at 90°C placing the PP tubes on Teflon heating blocks after adding 2 mL hydrochloric acid and 1 mL nitric acid (10 M HCl and 14 M HNO3, respectively, both Suprapur, Merck) following protocol adapted from (62 ...
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bioRxiv - Cell Biology 2023Quote: ... 2020) containing 300 ng GST-tagged MP-GAP fragments and 200 ng recombinant human Aurora A kinase (Merck, 14-511), 1mCi of [gamma-P32]ATP (Hartmann Analytic ...
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bioRxiv - Bioengineering 2023Quote: Microvessels cultured under different flow conditions were perfused at day 7 or 14 with 70 kDa FITC-labelled dextran (Merck) by the application of a hydrostatic pressure drop across the central gel channel ...
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bioRxiv - Neuroscience 2022Quote: ... Cubes of 1 cm3 were cut and incubated overnight in an aqueous solution of 150 mM NaCl (S9888, CAS 7647-14-5, Merck) and 10 µM fluorescein (46955 ...
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bioRxiv - Microbiology 2023Quote: ... Conidia from 14-day-old fungal cultures grown on culture media agar plates were filtered through two layers of Miracloth (Merck) and incubated with Hoechst 33342 solution (20 mM ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were detected by incubation with primary antibodies (SOX2, Bio-techne, AF2018, 1: 1000 and α-Tubulin, Merck, T6199, 1:2000) and secondary antibodies (Donkey anti-Goat IRDye® 800CW ...
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bioRxiv - Cell Biology 2024Quote: ... of cell lysates by incubating lysates with 0.5-2.5 µg of antibodies and 20 µl of protein G–Sepharose (Merck, GE17-0618-01) at 4°C for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... Cells were permeabilized with 100% ice-cold methanol for 20 min at -20°C and rehydrated by washing with PBS and then stained for viral hexon protein expression with an anti-hexon primary antibody (mouse; Merck; #MAB8052) and an anti-mouse secondary antibody coupled to AF488 or AF647 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were resolved on a 10% SDS-PAGE gel and Western blotting was performed using an anti-myc antibody (Merck, UK). Co-immunoprecipitation of Ssk2-HA was detected using an anti-HA antibody (Merck ...
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bioRxiv - Molecular Biology 2024Quote: ... and 300 µg of protein was then incubated with 1 µg of a mouse anti-53BP1 antibody (1μg/ml, Merck millipore, MAB3802) O/N at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
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bioRxiv - Bioengineering 2021Quote: Extracellular alkaline phosphate (ALP) qualitatively evaluated at D7/14/21 with Fast-Violet B Salt and Naphthol-AS-MX ALP staining (Merck KGaA). Cells fixed with a solution ...
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bioRxiv - Microbiology 2022Quote: m6A-seq was carried out as previously described [14] with minor chamnges .RNA of around 200 μg was fragmented using RNA fragmentation buffer (Merck #CS220011) at 94°C for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... Thirty µl of Protein G (Protein G Sepharose Fast Flow, Merck, UK) slurry per sample was added in addition to 0.01% sodium azide and incubated on a roller overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Total protein concentration was quantified by means of BCA protein assay (Merck). 10-20 μg of protein was separated by size using a 4-12% Bis-Tris Plus gel ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 µg/ml.
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bioRxiv - Cell Biology 2024Quote: ... Protein content was determined by performing a BCA protein assay (Merck Millipore). SDS-PAGE was performed on a Mini-PROTEAN System (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Cancer Biology 2024Quote: ... 09499-14) were subjected to SDS-PAGE on a 10% polyacrylamide gel and subsequently transferred to Immobilon-P PVDF-membrane (Merck Millipore, IPVH85R). The membrane was blocked with 5% (w/v ...