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Citations for Merck :
51 - 100 of 1194 citations for Recombinant Zika virus NS1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The recombinant protein was dialyzed overnight using an Amicon Ultra 15 mL spin dialysis column 10 000 MWCO (Merck, Germany). Purified protein was quantified using Bradford’s assay ...
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bioRxiv - Neuroscience 2023Quote: ... the affinity resin was prepared by incubating 100 µg of recombinant protein with 20 µl of nickel beads (PureProteome Nickel magnetic beads, Merck) in 50 mM Pull-Down Buffer PDB (Tris–HCl pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity-purified recombinant TrK13 proteins (WT, C580Y, R539T and C580Y) or commercially purchased BSA (A7030) or lysozyme (L6876) (Merck, Germany), each at 10 µM concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... the virus-containing supernatant (8ml) was filtered (Steriflip; Merck) before being concentrated 20x (14000 rpm ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The virus was concentrated in a 100KDa Amicon centrifugation unit (Merck) at 5000g for 15 mins to achieve a 10-fold concentration ...
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bioRxiv - Neuroscience 2021Quote: ... This concentrated virus solution was filtered (0.22 μm Millex, Merck Millipore) and stored at 4 °C until further use ...
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bioRxiv - Evolutionary Biology 2023Quote: ... virus samples were mixed with SYBR Green I stain (Merck, Germany) and Tris-EDTA buffer in a PCR plate ...
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bioRxiv - Cell Biology 2021Quote: ... human recombinant insulin (100 µg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant human TNFα (GF023) was from Merck. Purified anti-His tag mouse antibody was from BioLegend (USA) ...
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bioRxiv - Neuroscience 2020Quote: ... or 1:500 biotinylated Wisteria floribunda agglutinin WFA (#L8258; Merck, Germany), diluted in the same blocking solution ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing supernatants were filtered through a 0.45 μm membrane (Merck Millipore). The amount of virus in the supernatants was quantified by RT assay ...
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bioRxiv - Cell Biology 2022Quote: ... Virus amplification was performed in Sf9 TriEX cells (Novagen / Merck #71023-3) grown in TriEx media (Novagen / Merck #71022) ...
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bioRxiv - Genetics 2022Quote: ... Virus was concentrated using Amcion Ultra-15 30k centrifugal filter (Merck#C7715) and centrifugation for 30 min at 3,900rpm.
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bioRxiv - Genomics 2024Quote: ... virus-containing supernatant was filtered through a 0.45 μm filter (Merck Millipore), aliquoted ...
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bioRxiv - Pathology 2022Quote: ... recombinant anti-Cre (Merck Millipore, 69050, 1:1000) and revealed using Histofine reagent (Nichirei Biosciences) ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... previously coupled to 1 ml HI-TRAP NHS columns (GE/Merck) packed with Sepharose pre-activated for covalent coupling of ligands containing primary amino groups ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Physiology 2022Quote: ... and biotinylated donkey antibody to rabbit IgG (1:100; AP182B, Merck Millipore) for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg/mL (1:500 dilution) of biotinylated anti-phosphotyrosine 4G10 (Merck) antibody and incubated for 1 hour ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Physiology 2021Quote: ... The virus was further purified using Sepharose G100 SF resin (Merck, Darmstadt, Germany) in Econopac colums (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... Virus was concentrated in PBS using Amicon Ultra-15 Centrifugal Filter Units (Merck) and stored at 4°C.31,77,78 Viral titre was quantified by inverted terminal repeat probe quantitative polymerase chain reaction (PCR) ...
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bioRxiv - Biochemistry 2023Quote: ... Virus- containing supernatant P1 was sterile-filtered using a 0.22 μm filter (Merck). To amplify the virus 1.5 mL P1 was added to 250 mL of Sf21 shaking cell culture at a density of 0.4*106 cells/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 μg/ml of recombinant TNC (Merck Millipore) added to the medium ...
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bioRxiv - Immunology 2021Quote: ... recombinant hepatitis B surface antigen (HBsAg) manufactured by Merck & Co. ...
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bioRxiv - Biophysics 2020Quote: ... Recombinant DNA reagents and primers were purchased from Merck. As calibration samples in ALEX as well as anisotropy experiments ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 mg/mL Human recombinant Insulin (Merck, Cat. #91077C) and 1% Pen/Strep ...
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bioRxiv - Neuroscience 2023Quote: ... Tau ladder (six human tau recombinant isoforms, Sigma-Merck) was used to identify tau isoforms in NCI-H716 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1 IU/ml recombinant human FSH (Merck & Co., Inc), 0.125 mg/ml recombinant human hyaluronan (Novozymes) ...
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bioRxiv - Biochemistry 2023Quote: ... Active recombinant LKB1/STRADα/MO25α was purchased from Merck. Gateway pENTR plasmids encoding full length human BRSK1 & BRSK2 were generated as part of the NIH common fund initiative to Illuminate the Druggable Genome (IDG ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... and purified with Ni-NTA His•Bind® Resin agarose (Merck, 70666). The binding reaction was performed in 20 µL of binding buffer [10 mM Tris (pH 8.0) ...
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bioRxiv - Pathology 2021Quote: ... Biotinylated rhIL5 was produced using the Biotin-X-NHS system (Merck, Darmstadt, Germany). A total of 25 μL of rhIL5 (100 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl AAV virus was mixed with 1 μl 20% mannitol (MERCK K93152782 111). The virus and mannitol mixture were injected into a pulled-glass pipette (Warner Instruments ...
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bioRxiv - Biophysics 2023Quote: ... concentrated virus particles were freshly thawed and diluted in phosphate-buffered saline (PBS, Merck, 806552 ...
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bioRxiv - Molecular Biology 2021Quote: ... 6xHis-MPB-tagged 43GTPBP6 was expressed in E.coli BL21 (DE3) RIL cells (Merck Millipore) grown in LB media ...
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bioRxiv - Molecular Biology 2019Quote: ... protocol with the administration of recombinant FSH (Gonal-F, Merck Serono ...
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bioRxiv - Genetics 2020Quote: ... then rhCG (recombinant human chorionic gonadotropin alpha, OVIDREL, Merck Serono) on day 9 Oocytes were collected by laparoscopic follicular aspiration 32–35 h after rhCG administration ...
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bioRxiv - Biochemistry 2021Quote: ... A complex of recombinant bovine CaM (cat. no. 208690, Merck), with an amino acid sequence identical to the human isoform ...
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bioRxiv - Developmental Biology 2021Quote: ... Patients were injected with recombinant FSH (Gonal-F, Merck, Italy) on day 2 of their menstrual cycle with a starting dose of 150 IU/d ...
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bioRxiv - Immunology 2020Quote: ... and recombinant human RAP (Merck Millipore, 200 nM final concentration) were used to stimulate nuclear translocation of MerTK.
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bioRxiv - Biophysics 2022Quote: Lyophilized recombinant human Chorionic Gonadotropin (hCG) (Chorulon Merck #140-927) is reconstituted with the included sterile 1x PBS ...