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Citations for Merck :
51 - 100 of 411 citations for Recombinant Human CD3d Molecule Delta CD3 TCR Complex His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Immunology 2020Quote: ... 350 ng/mL active ERK2-GST tagged (Merck) or 175 ng/mL ERK1 plus 175 ng/mL ERK2-GST for 30 min at 30°C.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat (A0545; Merck-Sigma-Aldrich) was used as secondary antibody following the use of above antibody ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat (A0545; Merck-Sigma-Aldrich) was used as secondary antibody ...
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bioRxiv - Immunology 2019Quote: ... HLA molecules and peptides were eluted with 1% trifluoroacetic acid (TFA, Merck, Darmstadt, Switzerland) directly into Sep-Pak tC18 100 mg Sorbent 96-well plates (Waters ...
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bioRxiv - Plant Biology 2019Quote: ... Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat (A0545; Merck-Sigma-Aldrich) was used as secondary antibody following the use of Phospho-p44/42 MAPK antibody.
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bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
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bioRxiv - Microbiology 2023Quote: ... A commercial mouse anti-DENV complex monoclonal antibody (MAB8705; Merck Millipore) diluted 1:200 in phosphate-buffered saline (PBS ...
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Contractile acto-myosin network on nuclear envelope remnants positions human chromosomes for mitosisbioRxiv - Cell Biology 2019Quote: Small molecule inhibitors were used with the following concentrations: 1NM-PP1 1µM (Merck Millipore 529581), RO-3306 10µM (Merck Millipore 217699) ...
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bioRxiv - Developmental Biology 2023Quote: ... medium was changed to N2B27 supplemented with growth factors and small molecule inhibitors: CHIR99201 (Merck), murine BMP4 (PeproTech 315-27) ...
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bioRxiv - Microbiology 2020Quote: ... coli onto a fresh plate of complex solid media (Merck LB 110283) containing selective antibiotics when appropriate ...
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bioRxiv - Biophysics 2019Quote: ... Protein complex was concentrated with Amicon® Ultra Centrifugal Filters (Merck Millipore) and further purified on a Superdex 200 10/300 GL size-exclusion column (GE Healthcare ...
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bioRxiv - Developmental Biology 2024Quote: ... the ARP2/3 complex inhibitor CK666 (Merck, 100 μM, 60-min incubation); the actin polymerization inducer and actin depolymerization inhibitor jasplakinolide (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... The following small molecule inhibitors were used at the indicated concentration: nocodazole (5 µM; Merck Millipore), CK-666 (100 µM ...
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bioRxiv - Microbiology 2020Quote: ... circular DNA molecules were amplified with random examers and TempliPhi DNA polymerase (Merck KGaA, Darmstadt, Germany) following manufacturer’s instruction ...
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bioRxiv - Biophysics 2021Quote: ... Purified complex was concentrated with an Amicon Ultra 30 K unit (Merck Millipore).
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bioRxiv - Microbiology 2020Quote: ... A single colony was then incubated in liquid complex media (Merck LB 110285) overnight cultures ...
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bioRxiv - Molecular Biology 2022Quote: ... The complex was concentrated using ultrafiltration through a 100 kDa cutoff filter (Merck) and analyzed on a 10% SDS PAGE for the presence of Cas subunits ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Molecules larger than 10 kDa were concentrated using amicon ultra-15 centrifugal filters (Merck Millipore, Merck, USA). Concentrated supernatants were frozen at −80°C until further use and are further referred to as secretomes.
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bioRxiv - Microbiology 2019Quote: ... Molecules larger than 10 kDa were concentrated using amicon ultra-15 centrifugal filters (Merck Millipore, Merck, USA). Concentrated supernatants were frozen at −80°C until further use and are further referred to as secretomes.
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bioRxiv - Cell Biology 2021Quote: ... Myc-tagged proteins were immuno-precipitated with the 9E10 antibody (Merck); Rpb1 was immunoprecipitated using the 8WG16 antibody (Merck).
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with a mouse anti-DENV complex monoclonal antibody (MAB8705, Merck Millipore), washed three times with PBS ...
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bioRxiv - Pathology 2022Quote: ... recombinant anti-Cre (Merck Millipore, 69050, 1:1000) and revealed using Histofine reagent (Nichirei Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant active DAPK3 protein was obtained from Merck.
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bioRxiv - Biochemistry 2021Quote: ... The purified complex I was concentrated using an Amicon Ultra-4 100K centrifugal filter (Merck) to 0.5 mg mL-1 (w/v) ...
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bioRxiv - Biochemistry 2022Quote: ... mPSF:PAP complex was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 100 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Molecular Biology 2020Quote: ... The TDH complex was concentrated in a 100 kDa cut-off Amicon centrifugal concentrator (Merck) at 1,500 rcf to 0.1-0.2 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... the reducing agent 2 µl 2-picoline borane complex (PB, 2 M in DMSO, Merck) and 2 µl water ...
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bioRxiv - Biochemistry 2022Quote: ... The AstaPo1/AXT complex was concentrated by an Amicon concentrator with 3.5 kDa MWCO (Merck) to a concentration of 7 mg/mL (280 μM).
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bioRxiv - Immunology 2023Quote: ... MDMs were also pre-treated for 1h with a TLR3/dsRNA complex inhibitor (Merck Millipore) 5 µM final concentration ...
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bioRxiv - Biochemistry 2023Quote: ... instead the pyrene–actomyosin complex was incubated with 30 µM ADP (Merck KGaA, Darmstadt, Germany) for 20 minutes on ice prior to measurement ...
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bioRxiv - Molecular Biology 2024Quote: ... and protein complex was eluted in lysis buffer 2 containing 2.5 mM D-desthiobiotin (Merck). Protein tags were removed by overnight cleavage with HRV 3C protease ...
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bioRxiv - Cell Biology 2023Quote: ... Knockout of the molecules was confirmed by SDS-PAGE and western blotting using rabbit anti-ADAM10 polyclonal (Merck AB19026) at 1/500 followed by goat anti-rabbit HRP (BioRad ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... previously coupled to 1 ml HI-TRAP NHS columns (GE/Merck) packed with Sepharose pre-activated for covalent coupling of ligands containing primary amino groups ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Microbiology 2022Quote: ... and the FLAG-tagged proteins were immunoprecipitated with anti-FLAG agarose (A2220, MERCK). Input samples were collected as described above.
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bioRxiv - Molecular Biology 2024Quote: ... FLAG-tagged proteins were detected using M2 anti-FLAG antibody (mouse, F1084, Merck) followed and fluorescent Anti-Mouse Alexa Fluor™ Plus 800 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 μg/ml of recombinant TNC (Merck Millipore) added to the medium ...
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bioRxiv - Immunology 2021Quote: ... recombinant hepatitis B surface antigen (HBsAg) manufactured by Merck & Co. ...
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bioRxiv - Biophysics 2020Quote: ... Recombinant DNA reagents and primers were purchased from Merck. As calibration samples in ALEX as well as anisotropy experiments ...
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bioRxiv - Biochemistry 2023Quote: ... Active recombinant LKB1/STRADα/MO25α was purchased from Merck. Gateway pENTR plasmids encoding full length human BRSK1 & BRSK2 were generated as part of the NIH common fund initiative to Illuminate the Druggable Genome (IDG ...
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bioRxiv - Biochemistry 2022Quote: ... the complex was concentrated using a 30 kDa MWCO Amicon Ultra Centrifugal Filter Unit (Merck Millipore) and purified by gel filtration on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with filipin complex (Merck, cat #F-9765; 0.15 mg/ML in D-PBS) for 2 h and after several washes in D-PBS were visualized by fluorescence microscopy ...