Labshake search
Citations for Merck :
51 - 100 of 394 citations for Recombinant Human ABHD15 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... Myc-tagged proteins were immuno-precipitated with the 9E10 antibody (Merck); Rpb1 was immunoprecipitated using the 8WG16 antibody (Merck).
-
bioRxiv - Pathology 2022Quote: ... recombinant anti-Cre (Merck Millipore, 69050, 1:1000) and revealed using Histofine reagent (Nichirei Biosciences) ...
-
bioRxiv - Cell Biology 2023Quote: ... Recombinant active DAPK3 protein was obtained from Merck.
-
bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
-
bioRxiv - Cell Biology 2021Quote: ... previously coupled to 1 ml HI-TRAP NHS columns (GE/Merck) packed with Sepharose pre-activated for covalent coupling of ligands containing primary amino groups ...
-
bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
-
bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
-
bioRxiv - Microbiology 2022Quote: ... and the FLAG-tagged proteins were immunoprecipitated with anti-FLAG agarose (A2220, MERCK). Input samples were collected as described above.
-
bioRxiv - Molecular Biology 2024Quote: ... FLAG-tagged proteins were detected using M2 anti-FLAG antibody (mouse, F1084, Merck) followed and fluorescent Anti-Mouse Alexa Fluor™ Plus 800 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 2 μg/ml of recombinant TNC (Merck Millipore) added to the medium ...
-
bioRxiv - Immunology 2021Quote: ... recombinant hepatitis B surface antigen (HBsAg) manufactured by Merck & Co. ...
-
bioRxiv - Biophysics 2020Quote: ... Recombinant DNA reagents and primers were purchased from Merck. As calibration samples in ALEX as well as anisotropy experiments ...
-
bioRxiv - Biochemistry 2024Quote: ... Active recombinant LKB1/STRADα/MO25α was purchased from Merck. Gateway pENTR plasmids encoding full length human BRSK1 & BRSK2 were generated as part of the NIH common fund initiative to Illuminate the Druggable Genome (IDG ...
-
bioRxiv - Microbiology 2021Quote: ... and the supernatant was incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
-
bioRxiv - Plant Biology 2023Quote: ... and purified with Ni-NTA His•Bind® Resin agarose (Merck, 70666). The binding reaction was performed in 20 µL of binding buffer [10 mM Tris (pH 8.0) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 6xHis-MPB-tagged 43GTPBP6 was expressed in E.coli BL21 (DE3) RIL cells (Merck Millipore) grown in LB media ...
-
bioRxiv - Biochemistry 2021Quote: ... A complex of recombinant bovine CaM (cat. no. 208690, Merck), with an amino acid sequence identical to the human isoform ...
-
bioRxiv - Developmental Biology 2021Quote: ... Patients were injected with recombinant FSH (Gonal-F, Merck, Italy) on day 2 of their menstrual cycle with a starting dose of 150 IU/d ...
-
bioRxiv - Microbiology 2024Quote: ... His tags were removed using a Thrombin Cleavage Capture Kit (Merck, Darmstadt, Germany).
-
bioRxiv - Developmental Biology 2021Quote: ... and 75 mIU/mL of recombinant follicle stimulating hormone (Merck Serono). The oocytes were randomly assigned to experimental groups ...
-
bioRxiv - Biochemistry 2022Quote: ... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
-
bioRxiv - Biochemistry 2020Quote: ... Cell lysates were supplemented with 500 U Recombinant DNAse (Merck 4716728001) and 100 mM PMSF ...
-
bioRxiv - Biochemistry 2022Quote: ... 1000 U/mL recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore) and 50 U/mL Penicillin–Streptomycin (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2020Quote: ... ESGRO recombinant mouse Leukaemia Inhibitory Factor (LIF) was obtained from Merck Millipore ...
-
bioRxiv - Biochemistry 2020Quote: ... Recombinant rabbit anti-pan-ADP-ribose binding reagent MABE1016 (Merck Millipore) was used 1:1000 ...
-
bioRxiv - Genomics 2020Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2020Quote: ... recombinant FSH (Gonal-f 75 UI, Merck Serono Europe Limited, Spain), urinary human menopausal gonadotropin (hMG ...
-
bioRxiv - Genomics 2022Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Recombinant proteins were obtained after expression with B834 (DE3) cells (Merck), with IPTG at 0.8 mM and 22°C overnight ...
-
bioRxiv - Microbiology 2021Quote: ... Human HDL (Merck Millipore) was incubated with NS1 for 1 hour at 37°C prior to the DSC experiments ...
-
bioRxiv - Immunology 2021Quote: Native human insulin (Merck) was pre-diluted in water to 1 mg/mL ...
-
bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
-
bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
-
bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore ...
-
bioRxiv - Cell Biology 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
-
bioRxiv - Biophysics 2023Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosetta 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 µg/ml kanamycin and 34 µg/ml chloramphenicol ...
-
bioRxiv - Biophysics 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
-
bioRxiv - Molecular Biology 2024Quote: ... or HIS-Select Nickel Affinity Gel in a gravity-flow column (Merck, Darmstadt, Germany), followed by washing with binding buffer with and without 1.3% Triton X-100 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
-
bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... the GFP-Trap bead bound fraction was incubated with recombinant TEV (Merck) overnight at 4°C ...
-
bioRxiv - Biophysics 2020Quote: ... 100 μl of 10 μg/ml recombinant protein G (Merck, Darmstadt, Germany) was added and incubated for 30 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were treated with recombinant mouse TNFα (10 ng/ml, Merck 654245).
-
bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
-
bioRxiv - Developmental Biology 2021Quote: ... 5.06% fibrinogen (human plasma; Merck), 3U/mL thrombin (Stago BNL ...
-
bioRxiv - Microbiology 2022Quote: ... Plasma-purified human thrombin (Merck) was then added to reactions as required from a 1 IU / μL stock ...
-
bioRxiv - Biophysics 2024Quote: ... human epidermal growth factor (Merck), hydrocortisone (Merck) ...