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Citations for Merck :
51 - 100 of 476 citations for Frizzled 8 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... anti-Mad1 clone BB3-8 (1:1000, Merck), anti-ZW10 (Rabbit ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with 8 mg/mL Polybrene Reagent (Merck) for 1h ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Biophysics 2023Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosetta 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 µg/ml kanamycin and 34 µg/ml chloramphenicol ...
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bioRxiv - Biophysics 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Molecular Biology 2023Quote: ... or HIS-Select Nickel Affinity Gel in a gravity-flow column (Merck, Darmstadt, Germany), followed by washing with binding buffer with and without 1.3% Triton X-100 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Immunology 2021Quote: Viral vectors were produced by triple transient transfection of HEK-293Ts in 100 mm plates using GeneJuice (Merck – 70967) with a total of 12.5 μg of DNA ...
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bioRxiv - Microbiology 2022Quote: ... and human embryonic kidney (HEK)293T cells (ATCC #CRL-3216) were maintained in Dulbecco’s Modified Eagle Medium with high glucose (Merck), supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293T cells were transfected at ∼40 % confluency using a 3:1 polyethylenimine (branched, average Mw ∼25,000 Da; Merck) to DNA ratio (30:10 μg ...
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bioRxiv - Neuroscience 2023Quote: ... HEK-293T and HuTu-80 cells were transfected with the pSpCas9-GFP-LRP10gRNA plasmid using GeneJuice® reagent (Merck) according to manufacturer’s specifications ...
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bioRxiv - Neuroscience 2023Quote: ... HEK-293T and HuTu-80 cells were transfected with the pSpCas9-GFP-LRP10gRNA plasmid using GeneJuice® reagent (Merck). After 48 h ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’ cyclic mono-phosphate (8-Br-cAMP, Merck B5386) and 1 μM medroxyprogesterone acetate (MPA ...
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bioRxiv - Systems Biology 2022Quote: ... or 8 M Urea (Merck Sigma, Cat. No. U5378) and phosphatase/protease inhibitors ...
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bioRxiv - Bioengineering 2022Quote: ... in 150 mM NaHCO3 buffer (Merck, #144-55-8) with a pH of 11 ...
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bioRxiv - Developmental Biology 2023Quote: ... 2.25ml of BSA solution 35% (Merck 9048-46-8).
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were denatured with 8 M urea (Merck, U0631) in 0.1 M Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: Human fibronectin was from Merck Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... 5.06% fibrinogen (human plasma; Merck), 3U/mL thrombin (Stago BNL ...
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bioRxiv - Microbiology 2022Quote: ... Plasma-purified human thrombin (Merck) was then added to reactions as required from a 1 IU / μL stock ...
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bioRxiv - Biochemistry 2020Quote: ... epithelium suspension cell line expressing the EBNA-1 protein (female origin) was cultured in EX-CELL(r) 293 Serum-Free Medium (Merck, 14571C) containing 4 mM GlutaMAX supplement (TermoFisher ...
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bioRxiv - Plant Biology 2022Quote: ... The filtered supernatant was loaded onto a column packed with cOmpleteTM His-tag purification resin (Merck). His6-proteins were eluted with 4x 2 bead volumes of elution buffer (20 mM Na HEPES ...
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bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1 M 8-Bromoadenosine 3’5’-cyclic monophosphate (cAMP; Merck, B5386), 0.1 M 3-Isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Neuroscience 2020Quote: ... cells were grown in 8-well chamber slides (Merck, C7057), fixed in 10% Neutral Buffered Formalin for 30 minutes at RT ...
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bioRxiv - Genetics 2019Quote: ... 8) anti-CREB3L1 mouse monoclonal antibody 44c7 (Merck Millipore, MABE1017) (dilution 1:1000) ...
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bioRxiv - Genetics 2021Quote: ... 1 mM EDTA pH 8 + protease inhibitor cocktail (Merck Millipore), centrifugated 1000g x 10 minutes ...
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bioRxiv - Genetics 2023Quote: ... in the presence of 8 μg/mL Polybrene (Merck Millipore). After 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 0.2% BSA (Merck, Cat. no. 9048-46-8), here referred to as Dulbecco-BSA ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with 0.5 mM 8-bromo-cAMP (Merck), in combination with MPA ...
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bioRxiv - Immunology 2022Quote: ... Human heat-aggregated IgG immune complexes (ICs) were prepared by heating human IgG1 isotype control (Merck) for 20 min at 63°C.
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bioRxiv - Bioengineering 2019Quote: ... human fibronectin (Merck-Millipore, Watford, UK) was premixed with Laponite gels to a final concentration of 50 µg/mL ...
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bioRxiv - Microbiology 2019Quote: ... Binding of human FH (Merck Millipore) was detected with Alexa-Fluor-488 labeled human FH ...
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bioRxiv - Immunology 2022Quote: ... Purified C1q from human serum (Merck) was used as a control.
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bioRxiv - Systems Biology 2020Quote: ... anti-human MLKL (Merck Millipore, MABC604), phospho anti-human RPB1 S2 (Millipore ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... Human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 μg/μl for a 1h incubation at 37°C ...
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bioRxiv - Immunology 2023Quote: Human IFNβ (IF014, Merck, KGaA, Darmstadt), IFNα2a (11100-1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 μg/ml human insulin (Merck), and 50 μM hydrocortisone (Cayman ...
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bioRxiv - Immunology 2024Quote: ... Human MC lines HMC-1.1 (Merck) and HMC-1.2 (Merck ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then incubated with 2 mL pre-washed HIS-Select Cobalt Affinity Gel (Merck, UK) with rotation for an hour ...
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bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 0.005M calcium chloride and 8% sterile egg yolk enrichment (Merck, USA). A 5µl aliquot of C ...
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bioRxiv - Microbiology 2021Quote: ... in the presence of 8 μg/mL of polybrene (Merck Millipore). Virus supernatants were removed 7 h post-spin inoculation and cells were maintained in fresh growth medium for 48 h prior to selection in 3 μg/mL puromycin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... in the presence of 8 μg/mL of polybrene (Merck Millipore). 3 µg/ml Puromycin (Gibco ...