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Citations for Merck :
51 - 100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The cell pellet was then lysed in 5X volumes of Lysis Buffer (300μL) [50mM Tris pH 8 (Merck, T6066), 150mM NaCl (Merck ...
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bioRxiv - Molecular Biology 2024Quote: PLA was performed by using Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Merck, DUO92101-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The complete medium was degassed using aspiration in a Steriflip-HV cup (Merck, #SE1M003M00). All reagents and PBMC were maintained on ice before seeding ...
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bioRxiv - Molecular Biology 2024Quote: ... 300 kDa MWCO cassette (Merck) before loading onto a pre-equilibrated POROS CaptureSelect AAVX affinity column (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting cell pellet was freeze-thawed thrice and re-suspended to 10% (w/v) in a lysis buffer containing 5% (v/v) Polysorbate 80 and 20 U mL−1 benzonase (Merck). After 1 h incubation at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 kDa MWCO concentrator (Merck) before storage at −80 °C.
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bioRxiv - Microbiology 2024Quote: ... a volume of 20 mL with an OD720 of 0.6 was withdrawn from the culture grown in the desired conditions and vacuum filtered through a Durapore® 0.45 µm PVDF membrane (Merck Millipore-Ireland). The filter with the biomass was immediately inverted into a petri dish with 1.6 ml of pre-cooled methanol for quenching and incubated at −80°C for an hour with periodic rubbing of the filter against the container to aid cell lysis ...
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bioRxiv - Molecular Biology 2024Quote: The strains and samples were extracted using the RED Extract Plant PCR kit from Merck KGaA ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting pellet was retained and subject to extraction following the RED Extract Plant PCR-Kit (Merck KGaA, Darmstadt, Germany) protocol.
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bioRxiv - Molecular Biology 2024Quote: ... and homogenised in the Precellys 24 Tissue Homogeniser in 1X RIPA solution (Merck-Millipore) before being centrifuged at 17 000 g for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from cultures that finished sexual development using GeneElute – Mammalian Genomic DNA Miniprep Kit (Merck-Sigma, Germany). PCRs were done on specific genomic regions flanking an IES (Table S6 ...
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bioRxiv - Molecular Biology 2024Quote: ... and TMPyP4 were purchased from Merck and TCI Chemicals and were used as they were without further purification ...
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bioRxiv - Molecular Biology 2024Quote: ... to shear to an average size of 1,000–1,500 bp and incubated in the presence of BG4 (Merck, Milan, Italy, MABE817, Lot: 3534080). Anti-FLAG antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Fmoc-protected amino acids and resin for SPPS were bought from Novabiochem® (Merck, Milan, Italy), while Fmoc/Bhoc-protected PNA monomers were from Panagene (Daejeon ...
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bioRxiv - Molecular Biology 2024Quote: ... The second plaque slice was preserved in 2% PFA (8.18715.1000, Merck Millipore) and used for optical tissue clearing.
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bioRxiv - Molecular Biology 2024Quote: ... filtered through a 0.45 µm Millex-HV filter (Merck, Cat# SLHV033RS), and infected the 293T cells with 8 µg/ml polybrene (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... and 20% methanol (Merck, 32213-2.5L-M) for 1h before washing by ddH2O ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 × 107 wild-type U2OS or AsiSI-ER U2OS cells were crosslinked with 1% formaldehyde (Merck, 252549-100ML) for 10 min at 37°C and inactivated by the addition of glycine (Merck ...
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bioRxiv - Microbiology 2024Quote: ... ECL kits and absolute ethanol were from Merck-Millipore (Germany) ...
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bioRxiv - Microbiology 2024Quote: ... anti-Ras (Clone RAS10, #05-516, Merck-Millipore) at 1:1000 dilution and anti-G6PDH (A9521 ...
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bioRxiv - Microbiology 2024Quote: ... and the chloroform phase was separated by one-dimensional TLC on high performance TLC aluminum sheets (silica gel 60; Merck Poole, United Kingdom) and developed with chloroform/methanol/ammonium hydroxide (40:10:1 ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then concentrated through an Amicon Ultra-4 Filter Unit (Merck, cat#UFC8100214). The validation of the cytoplasm fraction was performed by western blot using antibodies against markers for nucleus (H2A) ...
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bioRxiv - Microbiology 2024Quote: ... The blots were probed using ECL reagent (Luminata Forte, WBLUF0100, Merck-Millipore).
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bioRxiv - Microbiology 2024Quote: ... at 1:1000 dilution and anti-G6PDH (A9521, Merck-Millipore) at 1:8000 dilution ...
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bioRxiv - Microbiology 2024Quote: ... and subsequently eluted in 150 μL of wash buffer with 500 ng/μL FLAG peptide (Merck, Darmstadt, Germany) by rotation on a rotator for 2 h at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... peptides were cleaned using in-house prepared C18 tips (66883-U-Merck).
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bioRxiv - Cell Biology 2024Quote: ... The activation was achieved using 50 μL of the initial microbead’s solution following the two-step 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide (EDC)/sulfo NHS covalent coupling (Estapor carboxyl-modified dyed microspheres protocol, Merck Millipore) in MES buffer (30 min ...
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bioRxiv - Microbiology 2024Quote: ... Samples were serially diluted and plated on MRS agar (Merck, Darmstadt, Germany) plates ...
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bioRxiv - Cell Biology 2024Quote: ... For super-resolution microscopy, samples were incubated with secondary antibodies (STAR635P (Abberior, 1:200) and Atto594 (Merck KgaA, 1:200)) ...
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bioRxiv - Cell Biology 2024Quote: pHLFs were lysed using ice-cold PhosphoSafe buffer (Merck Millipore) supplemented with protease inhibitors (cOmpleteMini ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were used against CD9 (Merck Millipore, CBL162, 1:1000), CD63 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-γ-H2AX (Merck, 05-636, 1 mg/ml, dilution 1:1000). For the secondary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... Purified proteins were concentrated using 10 kDa cutoff Amicon Ultra Centrifugal Filters (Merck Millipore) and stored in 50% v/v glycerol at -20 °C until further use.
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bioRxiv - Microbiology 2024Quote: ... or pET28b(+) vector (Merck Millipore) (LDH) ...
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bioRxiv - Microbiology 2024Quote: ... Amplified genes were digested and inserted into the pET28b(+) vector (Merck Millipore) (NDK ...
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bioRxiv - Microbiology 2024Quote: ... FC70 or FC40 (Merck Millipore) as the continuous phase ...
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bioRxiv - Microbiology 2024Quote: Western blot analysis was carried out by semidry electroblotting on immobilon-P transfer membrane from Merck KGaA (Darmstadt ...
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bioRxiv - Microbiology 2024Quote: Chemicals were purchased in analytical grade from Merck KGaA (Darmstadt ...
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bioRxiv - Microbiology 2024Quote: ... Transconjugant colonies carrying pCPE16_3blaNDM-1 were selected on LB agar supplemented with 300 µg/mL hygromycin B (PhytoTech Labs, USA) and 2 µg/mL doripenem (Merck, Germany). Conjugation frequencies were calculated using the following formula: Data shown are the mean ± standard deviation of three independent experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... protected by a Supelco ColumnSaver particle filter (Merck) and a gradient of mobile phase A (5 mM NH4OAc in CH3CN/H2O (40/60 ...
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bioRxiv - Cell Biology 2024Quote: Approximately 1×106 trophozoites of the amoeba transformants were cultured in a 10-cm dish using BIS medium under a low oxygen environment provided by Anaerocult (Merck) and incubated at 35.5 °C for 48 hours ...
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bioRxiv - Cell Biology 2024Quote: ● Sodium chloride (NaCl) (Merck).
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bioRxiv - Cell Biology 2024Quote: ● Stericup® 500 mL vacuum filters (Merck).
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bioRxiv - Molecular Biology 2024Quote: ... then layered over 5ml of 70% Percoll in a 15ml tube (Percoll Merck P4937, 10% 10x PBS, 20% RPMI). The tube was then centrifuged at 1450rcf for 11mins with the break set to 1 and accelerator to 3 ...
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bioRxiv - Molecular Biology 2024Quote: ... treated or not with 250 U/ml benzonase (Merck-Millipore) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... H3K27ac antibody (07-360; Merck), H3K27me3 (9733S ...
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bioRxiv - Molecular Biology 2024Quote: ... The starter culture was grown overnight at 37°C 250 rpm shaking and the following day 1 mL was used to inoculate 100 mL of AIM-TB (ForMedium) in a 250 mL baffled shake flask supplemented with 50 μg/mL kanamycin and 0.01% Antifoam-204 (Merck). Cells were grown first for 4 h at 37°C and then for a further 20 h at 18°C using 250 rpm shaking ...
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bioRxiv - Cell Biology 2024Quote: ... Blots were washed three times in TBS-T followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck). Following washing in TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-mediated substrate development was performed using Amersham detection system (ECL Western Blot detection kit, Merck). Blots were imaged using ChemiDoc™ MP Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... Following permeabilization with 2% TritonX (1.12298.0101, Merck Millipore) and blocking using 5% BSA (3737.4 ...