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Citations for Merck :
901 - 950 of 4341 citations for Tumor Protein Translationally Controlled 1 TPT1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The membranes were finally washed again 4 times for 10 min in TBS and revealed with Immobilon Western Blotting Chemoluminescence HRP substrate (Merck Millipore) and Super RX-film (Fujifilm ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
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bioRxiv - Biochemistry 2021Quote: ... After washing, the bound nanobody was detected by HRP-conjugated Goat anti-Alpaca IgG (Cat. S001P, NBbiolab) using Tetramethylbenzidine (TMB) (Merck, Cat.T2885) as a substrate for HRP ...
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were washed 3x in TBST for 10 min again and afterwards incubated in Immobilon ECL Ultra Western HRP Substrate (Merck, WBULS0100) for 2-5 min and scanned with GS-800 densitometer (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed four times for 5 minutes in PBST and incubated with Immobilon® Forte Western HRP Substrate (Merck, WBLUF0100) for 3 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were then washed with TBS-T three times for five minutes and exposed to Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) according to product instructions for 30 seconds before being developed ...
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bioRxiv - Molecular Biology 2022Quote: ... Glucocorticoid Receptor antibody (#3660, Cell Signalling Technology) or control IgG antibody (#03-198, Merck Millipore) and incubated for 1 h at room temperature whilst rotating ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies used in immunoblotting were as follows: rabbit polyclonal antibody anti-GAPDH (Merck Millipore #ABS16), mouse monoclonal antibody anti-PRKAR2B (#610625 ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were also stained with antibodies against the syncytiotrophoblast layer II marker monocarboxylate transporter 4 (MCT4; Merck Millipore AB3314P, used at 1:100) and against E-cadherin (CDH1 ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% BSA TBS-T or 5% skim milk TBS-T and incubated overnight at 4°C with the following primary antibodies diluted in 5 % BSA or 5% skim milk dissolved in TBS-T: pSer293-PDH (1:1,000) (Merck Millipore, Cat. no. ABS204), PDH (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Immunology 2023Quote: ... The embryos were subsequently incubated overnight at 4 °C in solution containing Cy3-conjugated α-smooth muscle actin monoclonal antibody (1:100, C6198; Merck, Darmstadt, Germany), Alexa647-Phalloidin (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue sections from the in vivo experiment were incubated with a rabbit primary antibody for GluR2/3 (Merck Millipore; AB1506; 1:200) overnight and then with a secondary goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Immunoreactive bands were detected using iBright CL1000 chemiluminator or LAS 3000 (Fujifilm, Tokyo, Japan) with an enhanced chemiluminescent substrate for the detection of horseradish peroxidase (HRP; Merck, Darmstadt, Germany). The intensity of the RAS band was quantified using ImageJ software and normalized to the intensity of the GAPDH band.
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bioRxiv - Biophysics 2021Quote: ... Pooled protein fractions were concentrated with Amicon-Ultra-15 (Merck KGaA) to 11.2 mg ml−1 as measured by the absorbance at 280 nm ...
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bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred to PVDF-FL transfer membrane (Merck Millipore) at 40V for 90 min at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined by Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Cell Biology 2022Quote: ... was used to transfer proteins to PVDF Immobilon membranes (Merck Millipore). Blocking and antibody incubation steps were performed in EveryBlot Blocking Buffer (BioRad) ...
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bioRxiv - Molecular Biology 2019Quote: Protein concentration was measured using a Direct Detect Infrared Spectrometer (Merck). 20 µg of proteins were separated on a NuPAGE 4-12% Bis-Tris gel (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2019Quote: ... and 100 Units/ml of Recombinant mouse LIF Protein (Merck ESG1107). Culture were seeded at an average density of ∼ 3.8*104 cells/cm2 and passaged every 48 h.
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bioRxiv - Microbiology 2019Quote: ... Proteins were electro-transferred to PVDF membranes (Merck Millipore, MA, USA). Immuno-detection was performed with polyclonal antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... the protein solution was filtered through 100 kDa filter (MERCK, MRCFOR100). The filtrate was transferred to a low-protein binding tube and 30 equivalents of HNE (Cayman Chem ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated in Amicon centrifugal filters (10 KDa, Merck) in presence of 1:10 GCDCA ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore). Densitometry was carried out by FIJI.
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bioRxiv - Cancer Biology 2019Quote: ... then incubated in lysis buffer containing Protein G Sepharose beads (Merck) and an antibody directed against DDR2 overnight at 4°C with rocking ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were transferred to an Immobilon-P Transfer Membrane (Merck), blocked with 10% Blocking One (nacalai tesque) ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were then transferred to a PVDF membrane (Merck Millipore) using a semi-dry western blot transfer system set to a constant current of 200 mA for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... proteins were transferred onto the PVDF membrane (Immobilon-FL, Merck-Millipore). After the electrotransfer ...
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bioRxiv - Cell Biology 2020Quote: Proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) using wet transfer ...
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bioRxiv - Biophysics 2022Quote: ... The proteins were concentrated using the Amicon Ultra centrifugal filters (Merck) by replacing the buffer with 20 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions were pooled and concentrated (10.000 MWCO Amicon; Merck-Millipore) up to 5 mL and analyzed on a HiLoad 26/60 Superdex 200 (GE ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein bands were detected using enhanced chemiluminescence (Luminata Crescendo, Merck Millipore). Membranes were re-probed with β-Actin antibody to ensure equal loading.
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bioRxiv - Biophysics 2021Quote: ... The proteins were concentrated with 50 kDa MWCO Amicon filters (Merck) to around 3-4 mg/ml for subsequent structural studies.
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bioRxiv - Developmental Biology 2022Quote: ... Protein bands were visualized in ECL solution (Merck KGaA, Darmstadt, Germany) using an ImageQuant LAS4000 (GE Healthcare) ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein was transferred to a 0.45 μm PVDF membrane (Merck Millipore) using BioRad transfer modules in transfer buffer (25 mM Tris ...
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bioRxiv - Bioengineering 2019Quote: ... Protein concentration was measured by the Direct Detect Spectrometer (Merck Millipore).
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bioRxiv - Cell Biology 2019Quote: ... Protein were transferred on nitrocellulose membrane (Amersham™ Protran®, Merck) at 80 V for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... The calibration mix (Protein Standard Mix 15 – 600 kDa, Merck Millipore) was composed of the following proteins ...
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bioRxiv - Bioengineering 2020Quote: ... Refolded proteins were concentrated with ultra centrifugal filters (Amicon®, Merck) with the appropriate mass cutoff and further purified by SEC.
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bioRxiv - Bioengineering 2020Quote: ... Eluted proteins were concentrated with ultra centrifugal filters (Amicon®, Merck) with the appropriated mass cutoff and further purified by SEC.
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bioRxiv - Cell Biology 2021Quote: ... The proteins were transferred to a PVDF membrane (Immobilon-P, Merck), blocked with 5% skim milk ...
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bioRxiv - Molecular Biology 2021Quote: ... protein concentration was quantified using a Direct Detect Infrared Spectrometer (Merck). 10 to 20 μg total protein was separated on a NuPAGE 4-12% Bis-Tris denaturing gel (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were then transferred to a PVDF membrane (Merck Millipore) using a semi-dry western blot transfer system set to a constant current of 200 mA for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Selected fractions containing homogeneous protein were concentrated using Amicon filters (Merck) in respective buffers (as described above ...
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bioRxiv - Molecular Biology 2021Quote: Protein concentration was measured using a Direct Detect Infrared Spectrometer (Merck). 20µg of proteins were separated on a NuPAGE 4–12% Bis-Tris gel (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were visualized using Coomassie Brilliant Blue R 250 (Merck, Germany) or using the SilverQuest silver staining kit (Invitrogen Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was determined using Direct Detect Infrared Spectrometer (Merck) and a solution with 20 µg of protein was heated at 95 °C for 5 min ...