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Citations for Merck :
901 - 950 of 1509 citations for Recombinant Rabbit KITLG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 50 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 50 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µM Cdc5Plk1 re-phosphorylated Mif2 protein (M3) was immobilized on anti-FlagM2 beads (Merck) and incubated with 25 µM Ame1/Okp1 complex in binding buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The proteins were concentrated to 50−100 µM using the Amicon Ultra centrifugal filters (Merck). For protein labelling ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein solution was applied to a MonoQ 10/100 column (Sigma-Aldrich, Merck), equilibrated in MonoQ buffer A (40 mM Hepes ...
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bioRxiv - Developmental Biology 2022Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Developmental Biology 2022Quote: ... The eluted proteins were then purified by Fractogel® EMD TMAE (Merck KGaA, Darmstadt, Germany) or SP-Sepharose (GE-Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... and then the proteins were transferred onto polyvinylidene difluoride membranes (Merck Millipore, Billerica, MA, USA).
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bioRxiv - Cell Biology 2022Quote: All proteins used in this study were overexpressed in Rosetta (DE3, pLysS) Escherichia coli (MERCK) cells using 0.4 mM isopropyl 1-thio-D-galactopyranoside (Inalco Pharmaceuticals ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins in the gel were then transferred to a poly-1,1-difluoroethane (PVDF) membrane (Merck) in transfer buffer (25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... proteins separated by SDS-PAGE were transferred to a PVDF membrane (Immobilon-P, Merck Millipore) in a Mini Trans-Blot (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: Protein lysates from cells were obtained by lysis in radioimmunoprecipitation assay buffer (Merck Millipore, #R0278) with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Microbiology 2019Quote: Protein samples were separated by SDS-PAGE and blotted on PVDF (polyvinylidenfluoride) membranes (Merck Millipore). Membranes were blocked for 1h with PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were transferred into methanol pre-activated polyvinylidene fluoride (PVDF) membranes (Immobilon-P, Merck-Millipore). After transference ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 30K (Merck Millipore) and stored at -80°C.
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bioRxiv - Microbiology 2020Quote: ... The purified protein was desalted and concentrated with the Centricon cutoff 10 kDa (Merck Millipore). The purity of the protein was analyzed by SDS-page and Western-blot using Anti-His antibodies.
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bioRxiv - Synthetic Biology 2020Quote: ... eluted protein fractions were concentrated using Amicon® Ultra-15 centrifugal filter units (Merck, USA) with a 100 KDa cut-off ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 100K (Merck Millipore) and stored at -80°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and the purified proteins were concentrated with Amicon Ultra-0.5 Centrifugal Filter devices (Merck Millipore) following manufactures instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was concentrated using a 30 kDa MWCO centrifugal filters (Amicon Ultra; Merck Millipore) and then gel filtered ...
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bioRxiv - Neuroscience 2021Quote: ... as confirmed by the anti-glial fibrillary acid protein (anti-GFAP) antibody (Merck, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2022Quote: RIP was performed according to the Magna-RIPTM RNA-binding protein immunoprecipitation kit (Millipore/Merck) as previously described (Papoutsoglou et al ...
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bioRxiv - Developmental Biology 2022Quote: ... L1s were then broken up to extract proteins using a Beadbug homogenizer (Merck Life Science) at the highest setting for 90 seconds three times at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were concentrated using 3 kDa MWCO Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore). Protein concentrations were determined using the Proteins and Labels function on a Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometer and subsequently confirmed on SDS-PAGE gel upon Coomassie staining and colorimetric imaging on an Amersham ImageQuant 800 CCD Imager.
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bioRxiv - Molecular Biology 2022Quote: The concentration of protein extracts was determined by BCA assay kit with BSA standards (Merck).
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bioRxiv - Microbiology 2023Quote: ... The proteins were separated via 15% SDS-PAGE and transferred to nitrocellulose membranes (Merck-Millipore). Western blot analyses were then conducted using PilA- ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein bands were visualised using a chemiluminescence substrate (Immobilon Forte, Merck Life Science UK Ltd.) on the ChemiDoc Imaging system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... eluted proteins were then concentrated using amicon ultra-15 centrifugal filters (Cat# UFC901024, Merck Millipore).
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bioRxiv - Plant Biology 2022Quote: ... 100 μg of protein were loaded into FASP filters (Amicon Ultra 30K, 0.5 ml, Merck) and tryptically digested over night following the FASP (Filter-Aided Sample Preparation ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 mins at 60°C with 10 mM dithiothreitol (Sigma, Merck) in 50 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously123 ...
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bioRxiv - Cell Biology 2023Quote: Protein extracts were transferred into an Amicon Ultra Centrifugal filter device (10 kDa MWCO, Merck) for buffer exchange [50 mM Tris-HCL pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 75 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Genetics 2023Quote: ... Anti-S6 Ribosomal Protein Antibody (used with 1:1000 dilution) was purchased from Merck (#ZRB1172). α-tubulin antibody (used with 1:5000 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Immunology 2023Quote: ... EVA71 VP0/VP2 reactive proteins were detected using anti-EVA71 VP2 antibody clone 979 (Merck) at 1:2000 dilution and anti-mouse IgG HRP conjugate ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to PolyVinyliDene Fluoride 0.45 µm membranes (Immobilon-Fl, Merck Millipore, Darmstadt, Germany). Membranes were saturated 1 hour with a Tris-Buffered Saline blocking solution (TBS blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to FL-PVDF membranes (cat. No. IPFL00010; Merck Millipore; Massachusetts, USA). The membranes were incubated with ms anti-Y1-R (1:500 ...
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bioRxiv - Physiology 2023Quote: ... The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously107 ...
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bioRxiv - Physiology 2023Quote: The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously23 ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were resolved via SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane (Merck, GE10600023) with a wet electroblotting system (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... Protein bands were visualized by treating the membrane with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated using SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-FL, Merck). The membranes were blocked for 30 min with Blocking-One or Blocking-One P (Nacalai Tesque ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were diluted in 1× BugBuster® (10× Protein Extraction Reagent, Novagen® Merck), 50 mM NaH2PO4 pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... Glycerol-free eluted protein was concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (Merck) at 10,000 x g ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was filtered through an Ultrafree-MC centrifugal device with hydrophilic PTFE membrane (Merck) at 10,000 x g ...
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bioRxiv - Molecular Biology 2024Quote: ... and protein complex was eluted in lysis buffer 2 containing 2.5 mM D-desthiobiotin (Merck). Protein tags were removed by overnight cleavage with HRV 3C protease ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was transferred to an Immobilon®-FL PVDF membrane (Merck Millipore, Billerica, MA, USA) and blocked in Licor blocking buffer for 120 minutes (Licor ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein eluates were then desalted and concentrated by ultrafiltration with Amicon Ultra 0.5 10K (Merck Millipore ...