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Citations for Merck :
901 - 950 of 1267 citations for Recombinant Human MMP1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing the proteins were concentrated with Amicon® Ultra-15 Centrifugal Filters Ultracel®-100K (Merck-Millipore ...
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bioRxiv - Microbiology 2019Quote: Ribonucleoprotein complex immunoprecipitation (RIP) was performed using the EZ-Magna RIP RNA-binding protein Immunoprecipitation Kit (Merck Millipore Billerica ...
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bioRxiv - Developmental Biology 2019Quote: ... were transformed with the pET-UBE2D3 plasmids and protein expression was induced with 0.4 mM IPTG (Merck) for 3h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted proteins were collected after a 72-h incubation by Amicon Ultra-15 Centrifugal Filter Units (Merck) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted protein was concentrated using an Amicon-Ultra-15 centrifugal filter (MWCO 30kDa) (Merck Millipore, USA), spin down 15 min in a bench-top centrifuge (4°C ...
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bioRxiv - Microbiology 2019Quote: ... Protein lysates were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride membranes (PVDF, Merck Millipore) for immunoblot analysis ...
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bioRxiv - Cell Biology 2021Quote: ... The separated proteins were then transferred to the PVDF membranes (0.45 µm Immobilon®-E; Merck Millipore). Membranes were blocked either with 2.5% nonfat milk in TBST (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... After eluting and concentrating the cleaved protein in Amicon Ultra-15 Centrifugal Filters 50K MWCO (Merck Millipore), size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2020Quote: ... Proteins in each lysate were resolved by SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Merck). The blots were incubated with the following primary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated by SDS-PAGE system and transferred to Immobilon-P membrane (Merck KGaA, Darmstadt, Germany). Immunoreactions were detected according to standard methods described previously [Yoshitake et al ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... proteins were separated with SDS-PAGE gels and transferred to PVDF membranes (Immobilon-P, Merck Millipore, USA). Blotted membranes were reacted with the specific primary antibodies shown in Supplementary Table 1 followed by peroxidase-linked secondary antibodies ...
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bioRxiv - Biochemistry 2022Quote: Protein extracts were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore-Merck, Germany). Membranes were blocked with 5% non-fat milk and incubated with a specific anti-biotin antibody overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the purified protein were concentrated using a 0.5 ml Amicon Ultra centrifugal filter (Merck Millipore) with a membrane molecular weight cut off 10 kDa.
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bioRxiv - Genetics 2022Quote: Whole-cell protein lysates were extracted in modified radioimmunoprecipitation assay buffer supplemented with protease inhibitors (#5892970001, Merck) and phosphatase inhibitor cocktail (#4906837001 ...
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bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... to 20 μg of mitochondrial protein and run with Tris-MOPS running buffer (#PCG3003; Sigma-Aldrich/Merck) on a 4-20% precast Trupage gel (#PCG2012-10EA ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were concentrated where necessary using Amicon filter devices with appropriate molecular weight cut-off (Millipore; Merck).
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd., Ireland). 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated by ultrafiltration using an Amicon Ultra-0.5 mL (10 kDa cutoff, Merck Millipore) at 14,000 × g and 4°C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were then sterilised by passing the eluate through a 0.2 μm filter (Merck Millipore), then concentrated using a 3kDa Vivaspin 20 centrifugal concentrator column (Sartorius ...
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bioRxiv - Immunology 2023Quote: ... Medium was collected after this period and filtered through 0.45μm ultra-low protein binding filter (Merck Millipore) to remove cell debris ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing purified protein were united and concentrated on Amicon-Ultra-15 10 K filtration units (Merck-Millipore ...
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bioRxiv - Biophysics 2023Quote: ... The TEV-cleaved protein was then buffer exchanged using a 30 kDa MWCO centrifugal filter (Amicon, Merck) into buffer containing no imidazole and 50 mM KCl (25 mM K·HEPES ...
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bioRxiv - Systems Biology 2023Quote: ... The separated proteins were transferred in a semi-dry system onto PVDF membranes (Immobilon P, Merck Millipore) using transfer buffer (192 mM glycine ...
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bioRxiv - Neuroscience 2023Quote: ... Protein-containing fractions were pooled and concentrated on an Amicon 3 kDa MWCO spin concentrator (Merck Millipore). After another IMAC purification step using Ni-NTA resin ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated using Amicon Ultra-15 centrifugal filters (100 kDa cut-off, Merck-Millipore). After treatment with 1 mM diamide for 15 min to completely oxidize the protein ...
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bioRxiv - Molecular Biology 2023Quote: ... PsC23 protein-containing fraction was collected and imidazole was removed by 10K centrifugal filter (Merck Millipore, Germany) using buffer (20 mM Tris-Cl ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were separated by reducing Tris-glycine SDS-PAGE and transferred to polyvinylidene difluoride (PVDF, Merck Millipore) membranes followed by enhanced chemiluminescence analysis (Advansta ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Biochemistry 2023Quote: ... Buffer exchange to 1xHBS and protein concentration was conducted by ultrafiltration (Amicon Ultra 10 kDa MWCO, Merck).
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated overnight with 30 μL ChIP-grade Protein G agarose beads (Merck Millipore, Germany) and 10 µg DNMT1 ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE gels were used for protein separation followed by transfer onto PVDF membrane (Merck, Cat. # IPFL00010). Blots were blocked with 5% BSA at room temperature for 1 h and incubated with the following primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal quantities of protein were separated on SDS-PAGE gels and transferred to the PVDF membranes (Merck), which were then blocked with 1 % nonfat skim milk in TBS-T for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to a Immobilion®-P Transfer Membrane (0.45 µm pore size, Cat#: IPVH00010, Merck) and blocked for 1h at RT in 1xTris Buffered Saline Tween (TBST ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S200 (16/600 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated by SDS-PAGE (7.5%–10% PAG) and transferred onto Immobilon-P membranes (Merck Millipore). Membranes were immunoblotted with the respective antibodies as follows ...
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-PAGE and then transferred to 0.45 μm PVDF membrane (IPVH00010, Merck Millipore). To keep the amounts of protein equally ...
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bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions containing target protein were desalted and concentrated on Amicon Ultra 10K centrifugal filter units (Merck Millipore), and the final protein preparation was stored in PBS containing 0.05% Triton X-100 at 4°C.
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bioRxiv - Microbiology 2024Quote: ... Target protein-containing fractions were combined and concentrated using an Amicon Ultra-4 50 kDa filter (Merck) operated at 7,500 x g and 4 °C ...
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bioRxiv - Physiology 2023Quote: ... 20 μg of total protein were transferred onto centrifugal filter units (Microcon-30 kDa; Merck, Darmstadt, Germany) and washed with a buffer containing 8 M urea and 100 mM Tris (pH 8.5) ...
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bioRxiv - Plant Biology 2024Quote: ... and proteins were concentrated using a 30 kDa Amicon Ultra-4 Centrifugal Filter Unit (Merck Milipore; www.merckmillipore.com). Protein concentration was determined employing the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... The fractions with target proteins were pooled and concentrated using an Amicon Ultra 3K NMWL (Merck Millipore). The sample was ultracentrifuged (45,000 rpm x 30 min ...
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bioRxiv - Bioengineering 2021Quote: Culture of human BM-MSCs and NE-MSCs with biomaterials performed in Corning® Costar® ultra-low attachment 24 well plates (Merck KGaA, Darmstadt, Germany) with Gibco™ α-MEM (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... Conjugated HEL-OVA solution was further purified from unconjugated proteins using a 50 kDa Amicon filter tube (Merck).
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bioRxiv - Immunology 2021Quote: ... Proteins were electrophoretically separated and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Merck Millipore, Burlington, MA, USA). Blocking treatment was performed using the PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing the target protein were concentrated to 5 ml with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was loaded onto a 320 ml HiLoad Superdex 75 (GE Healthcare Life Science ...
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bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... antibody complexes were purified by incubation of lysate with Protein A Sepharose® beads (Merck, GE17-5138-01) for 3h rotating at 4°C ...