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Citations for Merck :
901 - 950 of 2050 citations for Rat Interleukin 10 Receptor Beta IL10Rb ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... concentrated the next day using Amicon Ultra-15 concentrators MWCO 10 kDa (Merck Millipore, UFC901024), flash frozen and kept at -80°C.
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bioRxiv - Synthetic Biology 2022Quote: ... 100 mM NaCl) using an Amicon® Ultra-15 10 K Centrifuge Filter (Merck Millipore). The protein concentration was measured using the Qubit Protein Assay Kit and Qubit 3 Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... centrifuged at 2000xg for 10 min and resuspended in 25 ml RPMI-1640 medium (Merck) supplemented with 10 % FCS (FBS ...
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bioRxiv - Developmental Biology 2020Quote: ... reduced samples were resolved on 10% SDS-PAGE gels and blotted onto PVDF membranes (Merck). Membranes were blocked with 5% non-fat milk in PBS with 0.05% Tween-20 (Merck ...
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bioRxiv - Genetics 2021Quote: ... protease inhibitors (5 μM E64, 10 μM Bestatin, 5 μM Pepstatin, 48 hours (Merck Millipore)) ...
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bioRxiv - Biochemistry 2019Quote: ... 10 % v/v glycerol) and concentrated using an Amicon Ultra 15-mL 10k (Merck Millipore) spin column ...
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bioRxiv - Molecular Biology 2019Quote: ... and 10% glycerol) supplemented with a cocktail of protease inhibitors (1μg/mL leupeptin (Merck Millipore), 2μg/mL aprotinin (Merck Millipore) ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM MgCl2) and concentrated with 30-kDa MWCO centrifugal filters (Amicon Ultra, Merck Millipore). The proteins were flash-frozen in aliquots and stored at −80°C ...
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bioRxiv - Microbiology 2020Quote: ... The purified protein was desalted and concentrated with the Centricon cutoff 10 kDa (Merck Millipore). The purity of the protein was analyzed by SDS-page and Western-blot using Anti-His antibodies.
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bioRxiv - Microbiology 2021Quote: HeLa cells were obtained from Abcam (ab255928) and maintained in DMEM + (Thermo, 41966) supplemented with 10% heat-inactivated FBS (Merck, F7524) incubated at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... larvae were treated with pulses of 10 μM BrdU (Merck, Darmstadt, Germany, dissolved in E3) for various lengths of times depending on the experiment ...
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bioRxiv - Cell Biology 2021Quote: ... Enzymes were concentrated to ∼ 40 μM using Amicon Ultra centrifugal filters (Merck; MWCO 10 kDa) and flash frozen with liquid nitrogen for storage at -80°C.
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bioRxiv - Biochemistry 2021Quote: ... Blots were washed 3 x for 10 min with TBS-T (200 mM Tris (Merck), 1.36 mM NaCl (Merck) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were plated onto #1.5H coverslips coated with 10 μg/ml fibronectin (Merck, FIBRP-RO), 25 μg/ml laminin (Merck ...
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bioRxiv - Cell Biology 2021Quote: ... slides were washed 3×10 min in PBS and counterstained with PI (Merck, 1:500) and p-Phenylenediamine dihydrochloride (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... The eluate was concentrated (Amicon Ultra-15, 10 kDa MWCO, Millipore / Merck KgaA, Darmstadt, Germany), and buffer was exchanged by low-salt buffer (20mM TRIS ...
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bioRxiv - Biochemistry 2022Quote: ... 300 mM NaCl) using a 10 kDa MWCO Amicon Ultra Centrifugal Filter Unit (Merck Millipore). Complexes were aliquoted ...
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bioRxiv - Neuroscience 2022Quote: ... aSyn was concentrated using 10 k MWCO amicon centrifugal filtration devices (Merck KGaA, Darmstadt, Germany) and further purified to obtain monomeric aSyn using gel filtration on a HiLoad 16/60 75 pg Superdex column in 20 mM Tris pH 7.2 and stored at −80 °C until use ...
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bioRxiv - Microbiology 2022Quote: ... The sample was then injected into a Superose® 6 Increase 10/300 GL (Merck) column ...
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bioRxiv - Neuroscience 2022Quote: ... sections were blocked in 10 % normal donkey serum (NDS, v/v, Merck Millipore, Darmstadt, Germany) in 0.3 % PBS-Triton X-100 (PBS-T ...
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bioRxiv - Microbiology 2023Quote: Purified LDTGo was concentrated on a 10 kDa cutoff Amicon Ultra Centrifugal Filter (Merck-Millipore) to ∼10 mg/ml and loaded on a Superdex-200 increase 10/300 GL column equilibrated in 100 mM citrate/citric acid buffer pH 5.0 ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were diluted in 1× BugBuster® (10× Protein Extraction Reagent, Novagen® Merck), 50 mM NaH2PO4 pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... for 10 min at 37°C and inactivated by the addition of glycine (Merck, G7126) to a final concentration of 125 mM for 10 min (at 37°C) ...
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bioRxiv - Molecular Biology 2024Quote: ... 8 µL proteinase K (10 mg/mL) and 8 µL of 1 M DTT (Merck) was added to the sperm fraction which were vortexed and incubated at 56 °C for 60 min (mock casework samples ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were probed with an anti-poly-ADP-ribose (1:1000 in 10% milk, Merck) as well as anti- APLF and anti-GST as loading controls.
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bioRxiv - Biophysics 2023Quote: ... SK-N-BE cells expressing LAMP1-eGFP are treated with 10 µg/mL nocodazole (Merck) for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 75 (16/600 ...
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bioRxiv - Developmental Biology 2023Quote: ... that had been coated in for 45 minutes with 10 μg/mL bovine FN (Merck) followed by blocking with 5mg/mL bovine serum albumin (BSA) ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally (i.p.) with 10 mg/kg of azoxymethane (AOM, Sigma/Merck, A5486) in 0.9% NaCl) ...
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bioRxiv - Developmental Biology 2023Quote: ... the slides were incubated with 5 % BSA in TBST supplemented with 10% goat serum (Merck) for 1 h at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ... cells were cultured in RPMI 1640 (ccpro) supplemented with 10 % heat-inactivated human serum (Merck), 50 U/ml IL-2 (Miltenyi) ...
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bioRxiv - Bioengineering 2023Quote: ... followed by incubation in 10 mL of 2 U/mL dispase solution (D4693; Merck KGaA) at 4 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... and concentrated using a Centricon with a 10 kDa cutoff membrane (Merck Millipore, Burlington, MA). The concentrated sample was then purified by size exclusion chromatography using a HiLoad 26/600 Superdex 200 column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: Protein extracts were transferred into an Amicon Ultra Centrifugal filter device (10 kDa MWCO, Merck) for buffer exchange [50 mM Tris-HCL pH 7.5 ...
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bioRxiv - Bioengineering 2023Quote: ... The differentiation medium (DM) was prepared by supplementing 10 μM retinoic acid (RA; Merck, Germany) in the CM ...
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bioRxiv - Bioengineering 2024Quote: ... PL and PLMA by dissolving them at 10 mg.mL−1 in deuterium oxide (D2O, Merck) or in dimethyl sulfoxide-d6 (DMSO-d6) ...
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bioRxiv - Genetics 2024Quote: ... Proteins were then separated using 10% SDS-PAGE and transferred to PVDF membranes (Merck Millipore). PVDF membranes were then blocked by 5% BSA ...
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bioRxiv - Genetics 2024Quote: ... Proteins separated by 10% SDS-PAGE were transferred onto a PVDF membrane (Immobilon-E, Merck) and blocked in 5% milk in Tris-Buffer Saline (TBS ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg of RNA was reverse transcribed using a First Strand cDNA Synthesis Kit (K1622; Fermentas, Burlington, ON, Canada) and polymerase chain reaction (PCR) using a SYBR Green qPCR kit (Merck, Darmstadt, Germany) with incubation at 37°C for 60 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 50 hpf zebrafish embryos using the lysis buffer from the calpain 1/2 activity kit (InnoZyme™ Calpain 1/2 Activity Assay Kit CBA054 purchased from Merck Millipore). Protein concentration was adjusted to 2 mg/mL and calpain enzymatic activity was measured in microtubes following the manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... sterilized by UV irradiation for 30 min and coated with fibronectin (10 μg/ ml, Merck, #341631). Six to eight EM grids were placed in 35-mm Petri dishes and 300 μl of cell suspension was added (that is ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extensively washed with decreasing urea concentrations in ultracentrifugal filters (10 kDa MWCO, Amicon, Merck). RNA was recovered and subjected to NudC and alkaline phosphatase (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... Protein was transferred overnight at 10 V to an Immobilon-P membrane (Merck Millipore; Burlington, MA) in 25 mM Tris ...
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bioRxiv - Biophysics 2019Quote: ... the streptavidin tetramer mixture was concentrated in a stirred ultrafiltration cell (10 kDa cut-off, Merck). Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with Amicon Ultra-4 centrifugal filters (10 kDa cut-off ...
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bioRxiv - Immunology 2020Quote: ... After each cycle the flow channels were regenerated for 10 seconds with 2 M NaCl (Merck) at 10 µL/min ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... The eluent fraction was concentrated by ultra-filtration using Centriprep centrifugal filter YM-10 (Merck Millipore). Protein concentration was determined by Bradford assay (Bio-Rad).
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... the supernatant was concentrated by ultra-filtration using a Centriprep centrifugal filter YM-10 (Merck Millipore). The resultant solution (protein 15 mg/mL ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... The eluent fraction was concentrated by ultra-filtration using Centriprep centrifugal filter YM-10 (Merck Millipore). Protein concentration was determined by Bradford assay (Bio-Rad) ...
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bioRxiv - Biophysics 2021Quote: ... were first pre-treated with 5 and 10 μM concentrations of montelukast sodium hydrate (PHR1603, Merck) or saquinavir mesylate (1609829 ...