Labshake search
Citations for Merck :
901 - 950 of 2976 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... for 20 min at room temperature (RT) and permeabilised overnight at 4 °C in 0.5% Triton X-100 (Merck) solution in PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... MLg mouse lung fibroblasts (CCL-206, ATCC) were proliferation-inactivated by incubation in culture medium containing 10-μg·ml-1 mitomycin C (Merck) for 2 h ...
-
bioRxiv - Microbiology 2023Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 24 h at +4°C with primary antibodies (goat anti-Chat (1:200, Merck, AB144P), mouse anti-GFAP (1:300 ...
-
bioRxiv - Biochemistry 2023Quote: ... at 4°C (repeated for 10 cycles) to remove salt using an Amicon Ultra-0.5 Centrifugal 10 kDa filter unit (Merck), before dilution to 5.00 µM in 20 mM HEPES ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated by ultrafiltration (4°C, 3,500 x g) using Amicon Ultra Centrifugal Filter Units 10K molecular weight cut-off (MWCO) (Merck). CT produced in E ...
-
bioRxiv - Microbiology 2024Quote: ... samples maintained at 4°C were eluted through a ZIC-pHILIC column (5 μm, polymeric, 150 by 4.6 mm; SeQuant, Merck) by mobile phase A (20 mM ammonium carbonate ...
-
bioRxiv - Molecular Biology 2024Quote: ... The lysate was then spun at 20,000 g for 20 min at 4°C and loaded to a HisTrap HP column (Merck). The protein was eluted with the buffer with 300 mM NaCl ...
-
bioRxiv - Microbiology 2023Quote: ... 100 ml of the cell suspension at an OD660 of 0.6 was harvested by centrifugation at 5,000×g for 10 min at 4°C and resuspended in 2 ml of BugBuster (Merck), and 50 μg/ml lysozyme and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
-
bioRxiv - Biochemistry 2024Quote: The lysate was cleared by centrifugation at 12,000g for 30min at 10°C and the supernatant was applied onto a Talon Superflow beads batch (Merck). The immobilized proteins were washed with 50 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2024Quote: Unless otherwise noted the standard assay was carried out at 65 °C for 10 min in 500 μL reaction mixture using 0.25 % (w/v) laminarin from Laminaria digitata (Merck) as substrate in 50 mM sodium phosphate buffer pH 6.0 (adjusted at assay temperature) ...
-
bioRxiv - Cell Biology 2021Quote: ... the alkaline phosphatase detection kit (Merck) was used following manufacturer’s instructions.
-
bioRxiv - Neuroscience 2020Quote: ... Then they were incubated in mouse anti-Cx35/36 antibody (MAB3045, EMD Millipore, Merck, USA) at a dilution of 1:250 in blocking solution for 48 hours ...
-
bioRxiv - Neuroscience 2020Quote: ... membranes were incubated with anti-mouse antibody (1:5,000; Merck Millipore, Germany; Cat. No. AP124P). After incubation ...
-
bioRxiv - Neuroscience 2021Quote: The procedures for Cre-recombinase (anti-Cre-recombinase antibody; 1:500, mouse, MAB3120, Merck Millipore), and Fos-B (anti-FosB antibody 1:500 ...
-
bioRxiv - Neuroscience 2022Quote: ... using the following antibodies: mouse anti-NeuN (1:1500, Cat. # MAB377, Merck KGaA, Darmstadt, Germany), chick anti-GFP (1:2000 ...
-
bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used were the following: mouse anti-NeuN (1:500, RRID: AB_2298772, Merck Millipore) or rabbit anti-RFP (1:500 ...
-
bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were mouse monoclonal antibodies against actin (1:10000, Merck Millipore, MAB1501R), synaptophysin (1:5000 ...
-
bioRxiv - Neuroscience 2021Quote: ... and the other for neuronal nuclei (mouse anti-NeuN monoclonal antibody (Merck MAB377, 1:200)) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... a 1/5000 dilution of anti-mouse IgG conjugated with peroxidase (POD; Merck, MO, USA) was used to reveal the presence of bound anti-E-tag ...
-
bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal pS129 (11A5, 1:10,000) and tyrosine hydroxylase (TH, #AB152, Merck Millipore, 1:1000). Appropriate secondary Alexa Flour antibodies (Life Technologies and Abcam ...
-
bioRxiv - Biochemistry 2021Quote: ... then probed with anti-mouse or rabbit secondary antibody conjugated to HRP (Merck AP181P, AP132P) for 60 min at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: Mouse monoclonal antibodies used in this study were anti-β-actin (AC-15, Merck, A1978), anti-His tag (ThermoFisher Scientific ...
-
bioRxiv - Physiology 2023Quote: ... mouse monoclonal anti-phosphoserine conjugated to Duolink® PLA MINUS probe (DUO87012, 1:50, Merck).
-
bioRxiv - Molecular Biology 2023Quote: ... and Cy3-conjugated goat anti-mouse secondary antibody (diluted at 1:200, #AP124C; Merck Millipore) for detection ...
-
bioRxiv - Cell Biology 2023Quote: ... Mouse monoclonal anti vinculin (V9131) and rabbit polyclonal anti beta actin (A2066) were from Merck. Rabbit polyclonal antibodies to ARL8b (A88749) ...
-
bioRxiv - Developmental Biology 2024Quote: ... and LH were measured using the Mouse Pituitary Magnetic Bead Panel (MPTMAG-49K, Merck, USA) according to manufacturer instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... Organoid cells were counted using the Muse Cell Analyzer (Merck) and a total of 100,000 viable cells per well and in triplicates per treatment were seeded in conic v bottom 96-well plates (greiner bio-one) ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were detached with non-enzymatic cell dissociation solution (Merck) or briefly (for Itgb4 staining only ...
-
bioRxiv - Microbiology 2020Quote: ... through six consecutive dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with a 3 kDa or 10 kDa molecular weight cutoff (Merck). Protein complexes were assembled by mixing the subcomponents at the desired molar ratios ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... at 4°C in dialysis buffer (100 mM NaCl, 20mM Tris pH 7.4, containing 15 μl bovine thrombin (605157, Merck Millipore) to cleave the His-tags) ...
-
bioRxiv - Cell Biology 2019Quote: ... cut axially using a size 10 surgical scalpel into 1mm3 pieces and incubated at 37°C for 45 mins in LiberaseTM (Merck) and 10ul/ml DNAse I (Thermo Scientific™) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
-
bioRxiv - Molecular Biology 2021Quote: Heat-mediated antigen retrieval was performed for 3 min at 99°C in a 40mM trisodium citrate (Merck, Darmstadt, Germany) solution ...
-
bioRxiv - Cell Biology 2022Quote: ... Lysate was spun at 19650 x g for 20 min at 4 °C and the cleared supernatant was filtered in a 0.45 μm filter (Merck Millipore). M2 magnetic FLAG beads (Sigma-Aldrich ...
-
bioRxiv - Biophysics 2019Quote: ... Tag cleavage was carried out overnight at 4 °C on a roller with 10 units of HRV 3C protease (Novagen/Merck chemicals Boulevard Industrial Park ...
-
bioRxiv - Microbiology 2019Quote: ... coli strain strains were grown at 37°C in Lysogeny broth (LB) with shaking at 200 rpm or on agar (Merck). All meningococcal strains were grown on GC agar plates or GC broth (Difco ...
-
bioRxiv - Cancer Biology 2020Quote: ... 4°C) and the EV-depleted supernatant was filtered through a 0.22 μm pore PVDF Millex-HV filter (Merck Millipore) and analysed by NTA ...
-
bioRxiv - Neuroscience 2021Quote: ... After washing the sections were incubated overnight at 4°C with anti-NeuN antibody (1:1000, Merck Millipore, Cat. # ABN90P) in 1% normal goat serum and 0.1% Triton-X-100 in TBS ...
-
bioRxiv - Neuroscience 2021Quote: ... samples were heated 5 minutes at 95°C and 30µl were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
-
bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
-
bioRxiv - Cell Biology 2021Quote: ... cut axially using a size 10 surgical scalpel into 1mm3 pieces and incubated at 37°C for 45 mins in Liberase (Merck) and 10ul/ml DNAse I (Thermo Scientific) ...
-
bioRxiv - Systems Biology 2020Quote: ... The reaction was incubated at 25°C for 90 minutes followed by addition of 1 μL proteinase K (Merck, 3115887001) and incubation at 37°C for 10 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... then dialysed against the same stock of ITC buffer overnight at 4°C using 1 kDa Pur-a-lyzer tubes (Merck). Protein and RNA concentrations after dialysis were calculated by A280 and A260 absorbance respectively ...
-
bioRxiv - Physiology 2020Quote: ... blocked in 5% NGS and 0.5% Triton diluted in PBS and incubated over night at 4°C with primary antibody: rabbit anti-NG2 (1/50, AB5320, Merck), goat anti-PDGFRα (1/200 ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were washed in TBS and demi-water before being dried on a heat plate at 37°C before and cleared in xylene and mounted with Entellan (Merck).
-
bioRxiv - Cell Biology 2020Quote: ... pre-cleared extracts (2 mg) were incubated for 4 hr at 4 °C with 2 μg of pan–ADP–ribose binding reagent (MABE1016, Merck) or normal rabbit IgG (2729S ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
-
bioRxiv - Systems Biology 2022Quote: ... Protein precipitation was performed by addition of four volumes of ice-cold acetone (−20°C) (Merck Sigma, Cat. No. 650501) or TCA to a final concentration of 20% and proceeded for 1 hour at -20°C or 30 min at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: Phosphorylation of Sam68 N-term and C-term were obtained by incubation with CDK1/Cyclin B (Merck, catalogue 14-450) at a Cdk1:Sam68 ratio of 5:1000 at 20 °C ...